{"id":"https://openalex.org/W2418620165","doi":"https://doi.org/10.1007/978-1-61779-539-8_9","title":"Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers","display_name":"Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2418620165","doi":"https://doi.org/10.1007/978-1-61779-539-8_9","mag":"2418620165","pmid":"https://pubmed.ncbi.nlm.nih.gov/22328372","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11530137"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-61779-539-8_9","doi":"https://doi.org/10.1007/978-1-61779-539-8_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-539-8_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2491612826","display_name":"Host-Fungus Interactions"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11530137","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003041501","display_name":"Chiung-Yu Hung","orcid":"https://orcid.org/0000-0003-1091-3420","observed_orcids":["https://orcid.org/0000-0003-1091-3420"]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Chiung-Yu Hung","raw_affiliation_strings":["Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA. Chiungyu.Hung@UTSA.edu","Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA. Chiungyu.Hung@UTSA.edu","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA","institution_ids":["https://openalex.org/I45438204"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065393101","display_name":"Hua Zhang Wise","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hua Zhang Wise","raw_affiliation_strings":["Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA","Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA","institution_ids":["https://openalex.org/I45438204"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109144966","display_name":"Garry T. Cole","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I45438204","display_name":"The University of Texas at San Antonio","ror":"https://ror.org/01kd65564","country_code":"US","type":"education","lineage":["https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Garry T. Cole","raw_affiliation_strings":["Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA","Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, TX, USA","institution_ids":["https://openalex.org/I45438204"]},{"raw_affiliation_string":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, USA","institution_ids":["https://openalex.org/I45438204"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5003041501"],"corresponding_institution_ids":["https://openalex.org/I45438204"],"apc_list":null,"apc_paid":null,"fwci":1.093,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.78868217,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"845","issue":null,"first_page":"131","last_page":"147"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.6776999831199646,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.6776999831199646,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11037","display_name":"Fungal Infections and Studies","score":0.30709999799728394,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/coccidioides","display_name":"Coccidioides","score":1.0},{"id":"https://openalex.org/keywords/gene-disruption","display_name":"gene disruption","score":0.984000027179718},{"id":"https://openalex.org/keywords/gene-replacement","display_name":"gene replacement","score":0.7580000162124634},{"id":"https://openalex.org/keywords/hygromycin-resistance","display_name":"hygromycin resistance","score":0.8209999799728394},{"id":"https://openalex.org/keywords/phleomycin-resistance","display_name":"phleomycin resistance","score":0.996999979019165},{"id":"https://openalex.org/keywords/protoplast-preparation","display_name":"protoplast preparation","score":0.7639999985694885},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.777999997138977},{"id":"https://openalex.org/keywords/transformation","display_name":"transformation","score":0.5180000066757202},{"id":"https://openalex.org/keywords/haploid-nuclei","display_name":"haploid nuclei","score":0.6520000100135803},{"id":"https://openalex.org/keywords/arthroconidia","display_name":"arthroconidia","score":0.7720000147819519},{"id":"https://openalex.org/keywords/fungal-genetics","display_name":"fungal genetics","score":0.3700000047683716}],"concepts":[{"id":"https://openalex.org/C2780058085","wikidata":"https://www.wikidata.org/wiki/Q418735","display_name":"Hygromycin B","level":4,"score":0.6289584040641785},{"id":"https://openalex.org/C2781030883","wikidata":"https://www.wikidata.org/wiki/Q142764","display_name":"Coccidioides","level":2,"score":0.5802949070930481},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5077208280563354},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.46929389238357544},{"id":"https://openalex.org/C2778439347","wikidata":"https://www.wikidata.org/wiki/Q5139154","display_name":"Coccidioides immitis","level":2,"score":0.4361193776130676},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.41446900367736816},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.38898134231567383},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.045548439025878906}],"mesh":[{"descriptor_ui":"D002934","descriptor_name":"Cinnamates","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D003045","descriptor_name":"Coccidioides","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D003045","descriptor_name":"Coccidioides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005819","descriptor_name":"Genetic Markers","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D005819","descriptor_name":"Genetic Markers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006921","descriptor_name":"Hygromycin B","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D006921","descriptor_name":"Hygromycin B","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010692","descriptor_name":"Phleomycins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D025141","descriptor_name":"Drug Resistance, Fungal","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D025141","descriptor_name":"Drug Resistance, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1007/978-1-61779-539-8_9","doi":"https://doi.org/10.1007/978-1-61779-539-8_9","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-539-8_9","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:22328372","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22328372","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11530137","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11530137","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:11530137","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11530137","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Methods Mol Biol","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2","score":0.5365999937057495}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4344748344","display_name":"A Recombinant Protein Vaccine Against Coccidioidomycosis","funder_award_id":"R01AI071118","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G4698293448","display_name":"Coccidioidomycosis:Genome Comparison, Selection and Transcription.","funder_award_id":"R01AI070891","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G546720519","display_name":null,"funder_award_id":"R01 AI-0711178","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G6928970212","display_name":"Enhancement of protective efficacy of Coccidioides vaccines by adjuvants","funder_award_id":"R21AI114762","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W2012461553","https://openalex.org/W2013008148","https://openalex.org/W2055892974","https://openalex.org/W2057771065","https://openalex.org/W2074731671","https://openalex.org/W2098785804","https://openalex.org/W2103445583","https://openalex.org/W2114749809","https://openalex.org/W2115309710","https://openalex.org/W2127142464","https://openalex.org/W2127573804","https://openalex.org/W2137416653","https://openalex.org/W2144452188","https://openalex.org/W2151028042","https://openalex.org/W2162844540","https://openalex.org/W2166626547","https://openalex.org/W2169900015","https://openalex.org/W2170082195","https://openalex.org/W2266766322","https://openalex.org/W4214908531","https://openalex.org/W4236165009","https://openalex.org/W4242945259"],"related_works":["https://openalex.org/W2171849113","https://openalex.org/W2012461553","https://openalex.org/W2000583508","https://openalex.org/W2098091048","https://openalex.org/W4322499785","https://openalex.org/W1847378301","https://openalex.org/W7213442207","https://openalex.org/W2317243661","https://openalex.org/W2004867547","https://openalex.org/W2775910123"],"abstract_inverted_index":{"The":[0,25,83],"following":[1],"transformation":[2,81,111,117,142,223],"protocol":[3],"is":[4,143],"based":[5],"on":[6,184],"homologous":[7,120],"recombination":[8],"that":[9,33,64,158],"occurs":[10],"between":[11],"a":[12,20,147,169],"gene":[13,16,22,32],"disruption":[14],"or":[15,38,47,54,190],"replacement":[17],"construct":[18],"and":[19,98,164,224],"target":[21],"of":[23,67,96,115,141,176,208,220,226],"Coccidioides.":[24],"DNA":[26,85,105],"constructs":[27,86],"employed":[28],"contain":[29,160,181],"either":[30],"the":[31,45,68,80,91,94,116,151,162,177,195,216],"encodes":[34],"for":[35,60,79,194,218],"hygromycin":[36,188],"B":[37,53,189],"phleomycin":[39,55,191],"resistance,":[40],"which":[41,180],"are":[42,56,77,87,119,130,136],"present":[43],"in":[44,93,109,124],"pAN7.1":[46],"pAN8.1":[48],"plasmid":[49,84],"vectors,":[50],"respectively.":[51],"Hygromycin":[52],"used":[57,78],"to":[58,102,145,192,205],"select":[59],"transformants":[61],"at":[62],"concentrations":[63],"inhibit":[65],"growth":[66],"parental":[69,163],"strain.":[70,149],"Coccidioides":[71,122,134,221],"protoplasts":[72,92,153],"generated":[73],"from":[74],"germinated":[75],"arthroconidia":[76],"experiments.":[82],"taken":[88],"up":[89],"by":[90],"presence":[95],"calcium":[97],"polyethylene":[99],"glycol.":[100],"Twenty":[101],"100":[103],"transformants/\u03bcg":[104],"can":[106,200],"be":[107,201],"obtained":[108,202],"each":[110],"experiment.":[112],"Approximately":[113],"5-10%":[114],"events":[118],"recombinations.":[121],"cells":[123,157,183],"all":[125,133],"developmental":[126],"stages,":[127],"including":[128],"arthroconidia,":[129],"multinucleate.":[131],"Since":[132],"nuclei":[135],"haploid,":[137],"only":[138],"one":[139],"run":[140],"sufficient":[144],"create":[146],"mutant":[148],"However,":[150],"transformed":[152],"develop":[154],"into":[155],"heterokaryotic":[156,182],"typically":[159],"both":[161],"mutated":[165,196],"nuclei.":[166,197],"To":[167],"isolate":[168],"homokaryotic":[170,227],"strain,":[171],"we":[172,214],"perform":[173],"multiple":[174],"subcultures":[175,207],"single":[178],"colonies":[179],"selection":[185],"plates":[186],"with":[187],"enrich":[193],"Homokaryotic":[198],"mutants":[199],"after":[203],"three":[204],"four":[206],"isolated":[209],"colonies.":[210],"In":[211],"this":[212],"protocol,":[213],"describe":[215],"methodology":[217],"preparation":[219],"protoplasts,":[222],"isolation":[225],"mutants.":[228]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}