{"id":"https://openalex.org/W2417516865","doi":null,"title":"Isolation of L-forms from the spleens of Brucella suis-infected, penicillin-treated mice.","display_name":"Isolation of L-forms from the spleens of Brucella suis-infected, penicillin-treated mice.","publication_year":1981,"publication_date":"1981-05-01","ids":{"openalex":"https://openalex.org/W2417516865","mag":"2417516865","pmid":"https://pubmed.ncbi.nlm.nih.gov/7294609"},"language":"en","primary_location":{"id":"pmid:7294609","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7294609","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annales de microbiologie","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086876253","display_name":"J Schmitt-Slomska","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Schmitt-Slomska","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113537386","display_name":"R Caravano","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Caravano","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096658474","display_name":"M Anoal","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Anoal","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085359131","display_name":"Bernard Gay","orcid":"https://orcid.org/0000-0002-2793-9574","observed_orcids":["https://orcid.org/0000-0002-2793-9574"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B Gay","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"J Roux","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Roux","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.477,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.72964652,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"132","issue":"3","first_page":"253","last_page":"65"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brucella-suis","display_name":"Brucella suis","score":0.9300000071525574},{"id":"https://openalex.org/keywords/l-forms","display_name":"L-forms","score":0.972000002861023},{"id":"https://openalex.org/keywords/penicillin","display_name":"penicillin","score":0.8389999866485596},{"id":"https://openalex.org/keywords/spleen","display_name":"spleen","score":0.7070000171661377},{"id":"https://openalex.org/keywords/ultrastructure","display_name":"ultrastructure","score":0.7059999704360962},{"id":"https://openalex.org/keywords/biotype-1","display_name":"biotype 1","score":0.7929999828338623},{"id":"https://openalex.org/keywords/atypical-bacteria","display_name":"atypical bacteria","score":0.5950000286102295},{"id":"https://openalex.org/keywords/murine-infection","display_name":"mouse infection","score":0.5529999732971191}],"concepts":[{"id":"https://openalex.org/C2909519439","wikidata":"https://www.wikidata.org/wiki/Q836301","display_name":"Brucella suis","level":5,"score":0.8292742967605591},{"id":"https://openalex.org/C174802600","wikidata":"https://www.wikidata.org/wiki/Q12190","display_name":"Penicillin","level":3,"score":0.7905176877975464},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7833189368247986},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.6167841553688049},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.614490270614624},{"id":"https://openalex.org/C2780931953","wikidata":"https://www.wikidata.org/wiki/Q9371","display_name":"Spleen","level":2,"score":0.5446930527687073},{"id":"https://openalex.org/C2780767784","wikidata":"https://www.wikidata.org/wiki/Q313358","display_name":"Brucella","level":3,"score":0.5086715817451477},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.49415257573127747},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.4836200773715973},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.46479174494743347},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.2463790774345398},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.11429494619369507},{"id":"https://openalex.org/C2781429187","wikidata":"https://www.wikidata.org/wiki/Q156050","display_name":"Brucellosis","level":2,"score":0.07034561038017273},{"id":"https://openalex.org/C2777692091","wikidata":"https://www.wikidata.org/wiki/Q3512031","display_name":"Brucella melitensis","level":4,"score":0.04777461290359497},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002002","descriptor_name":"Brucella","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D002002","descriptor_name":"Brucella","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002002","descriptor_name":"Brucella","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D002006","descriptor_name":"Brucellosis","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007740","descriptor_name":"L Forms","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D007740","descriptor_name":"L Forms","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D007740","descriptor_name":"L Forms","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010400","descriptor_name":"Penicillin G","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:7294609","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7294609","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Annales de microbiologie","raw_type":"Journal Article"},{"id":"mag:2417516865","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7294609","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.7246000170707703,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1869183055","https://openalex.org/W1562386359","https://openalex.org/W1599159653","https://openalex.org/W2411082026","https://openalex.org/W1848480723","https://openalex.org/W2407060894","https://openalex.org/W2437757999","https://openalex.org/W1728836637","https://openalex.org/W2401558746","https://openalex.org/W1498214492"],"abstract_inverted_index":{"Previous":[0],"attempts":[1],"to":[2,22,89,184],"obtain":[3],"in":[4,58,129,147,165,191],"vitro":[5,148],"wall-deficient":[6,136],"stable":[7],"L-forms":[8,31,144],"of":[9,12,30,134,188],"various":[10],"strains":[11],"Brucella":[13,195],"have":[14],"failed":[15],"because":[16],"the":[17,28,90,135,186],"obtained":[18],"spheroplasts":[19],"revert":[20],"quickly":[21],"bacterial":[23,91],"form.":[24],"Here,":[25],"we":[26],"report":[27],"isolation":[29],"from":[32,156],"mice":[33],"infected":[34],"with":[35,44,53],"a":[36],"B.":[37,171],"suis":[38],"strain":[39],"type":[40],"1":[41],"and":[42,132,138,149,153],"treated":[43],"penicillin.":[45],"In":[46],"defined":[47],"experimental":[48,194],"conditions,":[49],"L-type":[50],"microcolonies":[51],"associated":[52,161],"tissue":[54,160],"debris":[55],"were":[56,77,145],"observed":[57],"primary":[59],"spleen":[60],"cultures,":[61],"even":[62,111],"on":[63,70,83,104,115],"antibiotic":[64],"free":[65],"media.":[66],"After":[67],"several":[68,113],"transfers":[69,103],"penicillin-containing":[71],"medium.":[72,117],"typical,":[73],"tissue-free":[74],"L":[75],"colonies":[76,87],"obtained.":[78],"At":[79],"first,":[80],"when":[81],"cultivated":[82],"antibiotic-free":[84,116],"medium,":[85,106],"these":[86],"reverted":[88,110],"form":[92],"(identified":[93],"as":[94,159,170],"B":[95],"suis,":[96,172],"biotype":[97,173],"1).":[98],"Later,":[99],"after":[100,112],"approximately":[101],"fifteen":[102],"penicillin-supplemented":[105],"they":[107],"no":[108],"longer":[109],"subcultures":[114],"The":[118,142],"L-forms'":[119],"ultrastructural":[120],"features":[121],"included":[122],"many":[123,139],"giant":[124],"empty":[125],"bodies,":[126],"considerable":[127],"variation":[128],"size,":[130],"shape":[131],"density":[133],"cells,":[137],"multilayered":[140],"membranes.":[141],"stabilized":[143],"propagated":[146],"inoculated":[150],"into":[151],"mice,":[152],"then":[154],"recovered":[155],"their":[157],"spleens":[158],"L-microcolonies.":[162],"An":[163],"occasional":[164],"vivo":[166],"revertant":[167],"was":[168],"identified":[169],"1.":[174],"These":[175],"data":[176],"provide":[177],"one":[178],"possible":[179],"explanation":[180],"for":[181],"earlier":[182],"failures":[183],"detect":[185],"presence":[187],"atypical":[189],"bacteria":[190],"clinical":[192],"or":[193],"infections.":[196]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}