{"id":"https://openalex.org/W2417371011","doi":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","title":"Flickery block of single CFTR chloride channels by intracellular anions and osmolytes","display_name":"Flickery block of single CFTR chloride channels by intracellular anions and osmolytes","publication_year":1996,"publication_date":"1996-08-01","ids":{"openalex":"https://openalex.org/W2417371011","doi":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","mag":"2417371011","pmid":"https://pubmed.ncbi.nlm.nih.gov/8770004"},"language":"en","primary_location":{"id":"doi:10.1152/ajpcell.1996.271.2.c628","doi":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033237248","display_name":"Paul Linsdell","orcid":"https://orcid.org/0000-0001-6257-6273","observed_orcids":["https://orcid.org/0000-0001-6257-6273"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P. Linsdell","raw_affiliation_strings":["Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056371233","display_name":"John W. Hanrahan","orcid":"https://orcid.org/0000-0001-9080-2039","observed_orcids":["https://orcid.org/0000-0001-9080-2039"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"J. W. Hanrahan","raw_affiliation_strings":["Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Physiology, McGill University, Montreal, Quebec, Canada.linsdell@medcor.mcgill.ca","institution_ids":["https://openalex.org/I5023651"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I5023651"],"apc_list":null,"apc_paid":null,"fwci":10.9034,"has_fulltext":false,"cited_by_count":74,"citation_normalized_percentile":{"value":0.98680004,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"271","issue":"2","first_page":"C628","last_page":"C634"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10665","display_name":"Cystic Fibrosis Research Advances","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10665","display_name":"Cystic Fibrosis Research Advances","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.2020999938249588,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.10300000011920929,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cftr","display_name":"CFTR","score":0.972000002861023},{"id":"https://openalex.org/keywords/cystic-fibrosis-transmembrane-conductance-regulator","display_name":"cystic fibrosis transmembrane conductance regulator","score":0.9850000143051147},{"id":"https://openalex.org/keywords/chloride-channels","display_name":"chloride channels","score":0.824999988079071},{"id":"https://openalex.org/keywords/single-channel-recording","display_name":"single-channel recording","score":0.6359999775886536},{"id":"https://openalex.org/keywords/outward-rectification","display_name":"outward rectification","score":0.7739999890327454},{"id":"https://openalex.org/keywords/glutamate","display_name":"glutamate","score":0.7260000109672546},{"id":"https://openalex.org/keywords/gluconate","display_name":"gluconate","score":0.9980000257492065},{"id":"https://openalex.org/keywords/osmolytes","display_name":"osmolytes","score":0.8209999799728394},{"id":"https://openalex.org/keywords/sucrose","display_name":"sucrose","score":0.8169999718666077},{"id":"https://openalex.org/keywords/sorbitol","display_name":"sorbitol","score":0.9980000257492065},{"id":"https://openalex.org/keywords/urea","display_name":"urea","score":0.9990000128746033},{"id":"https://openalex.org/keywords/voltage-dependent-block","display_name":"voltage-dependent block","score":0.8529999852180481},{"id":"https://openalex.org/keywords/chloride-concentration","display_name":"chloride concentration","score":0.5350000262260437},{"id":"https://openalex.org/keywords/channel-conductance","display_name":"channel conductance","score":0.7250000238418579}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7617474794387817},{"id":"https://openalex.org/C145822097","wikidata":"https://www.wikidata.org/wiki/Q106916978","display_name":"Chloride channel","level":2,"score":0.7595233917236328},{"id":"https://openalex.org/C2778428886","wikidata":"https://www.wikidata.org/wiki/Q420470","display_name":"Cystic fibrosis transmembrane conductance regulator","level":3,"score":0.679490327835083},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.6712243556976318},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.6214709281921387},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5490128397941589},{"id":"https://openalex.org/C2778695967","wikidata":"https://www.wikidata.org/wiki/Q44791900","display_name":"Chloride","level":2,"score":0.5222452282905579},{"id":"https://openalex.org/C50254741","wikidata":"https://www.wikidata.org/wiki/Q62536","display_name":"Ion channel","level":3,"score":0.43425536155700684},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39714038372039795},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10197228193283081},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.06768953800201416},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000838","descriptor_name":"Anions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004553","descriptor_name":"Electric Conductivity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004594","descriptor_name":"Electrophysiology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007425","descriptor_name":"Intracellular Membranes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019005","descriptor_name":"Cystic Fibrosis Transmembrane Conductance Regulator","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D019005","descriptor_name":"Cystic Fibrosis Transmembrane Conductance Regulator","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajpcell.1996.271.2.c628","doi":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpcell.1996.271.2.c628","pdf_url":null,"source":{"id":"https://openalex.org/S62704344","display_name":"American Journal of Physiology-Cell Physiology","issn_l":"0363-6143","issn":["0363-6143","1522-1563"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Cell Physiology","raw_type":"journal-article"},{"id":"pmid:8770004","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8770004","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1503847195","https://openalex.org/W1969196640","https://openalex.org/W1972554093","https://openalex.org/W1999640868","https://openalex.org/W2014899976","https://openalex.org/W2023130009","https://openalex.org/W2038589763","https://openalex.org/W2039599894","https://openalex.org/W2055252729","https://openalex.org/W2058535047","https://openalex.org/W2083317271","https://openalex.org/W2100432609","https://openalex.org/W2127000342","https://openalex.org/W2131573781","https://openalex.org/W2160546177"],"related_works":["https://openalex.org/W2154373279","https://openalex.org/W2148028731","https://openalex.org/W2092732807","https://openalex.org/W7096301928","https://openalex.org/W2411195256","https://openalex.org/W1990015724","https://openalex.org/W1964853280","https://openalex.org/W7098977623","https://openalex.org/W2092850037","https://openalex.org/W2132957145"],"abstract_inverted_index":{"Cystic":[0],"fibrosis":[1],"transmembrane":[2],"conductance":[3,176],"regulator":[4],"(CFTR)":[5],"is":[6,146,177],"a":[7,39,50,77,114,154,189,193],"phosphorylation-":[8],"and":[9,46,71,100,144,160,186],"nucleotide-dependent":[10],"chloride":[11,34,111,118,158],"channel.":[12,48],"Single":[13],"CFTR":[14,82,140,175],"currents":[15],"recorded":[16],"on":[17],"cell":[18],"show":[19],"slight":[20],"outward":[21,142],"rectification,":[22,143],"which":[23],"has":[24],"previously":[25],"been":[26],"suggested":[27,147],"to":[28,31,38,86,122],"be":[29],"due":[30],"an":[32],"asymmetrical":[33,157],"ion":[35],"gradient":[36],"or":[37],"specific":[40],"interaction":[41],"between":[42],"permeant":[43,68],"intracellular":[44,183],"anions":[45],"the":[47,66,72,87,91,98,101,109,128,164,197],"Using":[49],"single-channel":[51],"recording":[52],"from":[53,127,153],"Chinese":[54],"hamster":[55],"ovary":[56],"cells":[57],"stably":[58],"expressing":[59],"CFTR,":[60],"we":[61,172],"have":[62],"found":[63],"that":[64,148,174],"both":[65],"sparingly":[67],"anion":[69,74],"glutamate":[70],"impermeant":[73],"gluconate":[75],"cause":[76],"rapid,":[78],"voltage-dependent":[79,161],"block":[80,105,162,195],"of":[81,94,104,136,139,156,163,182,196],"channels":[83],"when":[84],"applied":[85],"intracellular,":[88],"but":[89],"not":[90],"extracellular,":[92],"face":[93],"excised":[95],"patches.":[96],"Both":[97],"affinity":[99],"voltage":[102],"dependence":[103],"were":[106],"affected":[107],"by":[108,166,179,199],"extracellular":[110],"concentration":[112],"in":[113,134,188],"manner":[115,190],"consistent":[116,191],"with":[117,192],"ions":[119,126],"being":[120],"able":[121],"repel":[123],"these":[124,200],"blocking":[125],"pore.":[129],"These":[130],"results":[131],"are":[132],"discussed":[133],"terms":[135],"previous":[137],"models":[138],"current":[141],"it":[145],"this":[149],"rectification":[150],"may":[151],"result":[152],"combination":[155],"concentrations":[159,181],"channel":[165,198],"large":[167],"cytoplasmic":[168],"anions.":[169],"In":[170],"addition,":[171],"find":[173],"decreased":[178],"high":[180],"sucrose,":[184],"sorbitol,":[185],"urea":[187],"rapid":[194],"molecules.":[201]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}