{"id":"https://openalex.org/W2417039155","doi":"https://doi.org/10.1385/1-59259-395-x:39","title":"Production of Randomly Mutated Plasmid Libraries Using Mutator Strains","display_name":"Production of Randomly Mutated Plasmid Libraries Using Mutator Strains","publication_year":2003,"publication_date":"2003-11-15","ids":{"openalex":"https://openalex.org/W2417039155","doi":"https://doi.org/10.1385/1-59259-395-x:39","mag":"2417039155","pmid":"https://pubmed.ncbi.nlm.nih.gov/12824600"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-395-x:39","doi":"https://doi.org/10.1385/1-59259-395-x:39","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-395-x:39","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Directed Evolution Library Creation","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2977462757","display_name":"Directed Evolution Library Creation"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027065009","display_name":"Annalee W. Nguyen","orcid":"https://orcid.org/0000-0003-1268-7164","observed_orcids":["https://orcid.org/0000-0003-1268-7164"]},"institutions":[{"id":"https://openalex.org/I154570441","display_name":"University of California, Santa Barbara","ror":"https://ror.org/02t274463","country_code":"US","type":"education","lineage":["https://openalex.org/I154570441"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Annalee W. Nguyen","raw_affiliation_strings":["Department of Chemical Engineering, University of California, Santa Barbara, USA","Department of Chemical Engineering, University of California, Santa Barbara"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Santa Barbara, USA","institution_ids":["https://openalex.org/I154570441"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Santa Barbara","institution_ids":["https://openalex.org/I154570441"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021477625","display_name":"Patrick S. Daugherty","orcid":"https://orcid.org/0000-0003-4583-751X","observed_orcids":["https://orcid.org/0000-0003-4583-751X"]},"institutions":[{"id":"https://openalex.org/I154570441","display_name":"University of California, Santa Barbara","ror":"https://ror.org/02t274463","country_code":"US","type":"education","lineage":["https://openalex.org/I154570441"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrick S. Daugherty","raw_affiliation_strings":["Department of Chemical Engineering, University of California, Santa Barbara","Department of Chemical Engineering, University of California, Santa Barbara, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Santa Barbara","institution_ids":["https://openalex.org/I154570441"]},{"raw_affiliation_string":"Department of Chemical Engineering, University of California, Santa Barbara, CA","institution_ids":["https://openalex.org/I154570441"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I154570441"],"apc_list":null,"apc_paid":null,"fwci":2.1904,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.90114943,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"231","issue":null,"first_page":"39","last_page":"44"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.43630000948905945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.43630000948905945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11764","display_name":"Evolution and Genetic Dynamics","score":0.3208000063896179,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.05770000070333481,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mutator-strains","display_name":"mutator strains","score":0.9620000123977661},{"id":"https://openalex.org/keywords/random-mutagenesis","display_name":"random mutagenesis","score":0.8560000061988831},{"id":"https://openalex.org/keywords/plasmid-library","display_name":"plasmid library","score":0.9950000047683716},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"directed evolution","score":0.796999990940094},{"id":"https://openalex.org/keywords/single-base-substitutions","display_name":"single base substitution","score":0.6919999718666077},{"id":"https://openalex.org/keywords/indels","display_name":"indels","score":0.9940000176429749},{"id":"https://openalex.org/keywords/diversity-in-libraries","display_name":"library diversity","score":0.4359999895095825}],"concepts":[{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.872016429901123},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.7070788741111755},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.688927412033081},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6707685589790344},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.48586317896842957},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47512322664260864},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4431209862232208},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4314136505126953},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.41415590047836304},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3408172130584717},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.12146452069282532},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015698","descriptor_name":"Genomic Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1385/1-59259-395-x:39","doi":"https://doi.org/10.1385/1-59259-395-x:39","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-395-x:39","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Directed Evolution Library Creation","raw_type":"book-chapter"},{"id":"pmid:12824600","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12824600","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W1598771428","https://openalex.org/W2013631501","https://openalex.org/W2027905552","https://openalex.org/W2033187951","https://openalex.org/W2043949252","https://openalex.org/W2053066561","https://openalex.org/W2094531548","https://openalex.org/W2101994201","https://openalex.org/W2112912294","https://openalex.org/W2136524498","https://openalex.org/W3025291808"],"related_works":["https://openalex.org/W178110801","https://openalex.org/W2435332183","https://openalex.org/W43016190","https://openalex.org/W279381267","https://openalex.org/W1591137876","https://openalex.org/W2417488974","https://openalex.org/W2395118359","https://openalex.org/W2097866867","https://openalex.org/W7100530840","https://openalex.org/W140325698"],"abstract_inverted_index":{"A":[0,80],"variety":[1],"of":[2,10,34,51,66,83,94],"methods":[3],"have":[4],"been":[5,59],"developed":[6],"for":[7,19],"random":[8,89,111],"mutagenesis":[9],"genes":[11],"and":[12,71],"whole":[13],"plasmids":[14,52],"to":[15,44,61,87],"generate":[16,45],"genetic":[17],"diversity":[18],"directed":[20],"evolution":[21],"experiments":[22],"(":[23,77],"1":[24],").":[25,79],"In":[26],"particular,":[27],"bacterial":[28],"strains":[29,57,86],"exhibiting":[30],"unusually":[31],"high":[32],"rates":[33],"spontaneous":[35],"DNA":[36,126],"mutagenesis,":[37],"or":[38,99,115,121],"mutator":[39,56,85],"strains,":[40],"can":[41,106],"be":[42],"used":[43],"large,":[46],"diverse":[47],"plasmid":[48,76],"libraries.":[49],"Propagation":[50],"within":[53],"relatively":[54],"stable":[55],"has":[58],"shown":[60],"provide":[62],"a":[63,109],"rich":[64],"spectrum":[65],"single":[67],"base":[68],"substitutions,":[69],"insertions,":[70],"deletions":[72],"throughout":[73],"the":[74,92,95],"entire":[75],"2":[78],"significant":[81],"advantage":[82],"using":[84],"create":[88,108],"libraries":[90],"is":[91],"accessibility":[93],"procedure.":[96],"Specialized":[97],"equipment":[98],"cloning":[100],"techniques":[101],"are":[102],"not":[103],"required.":[104],"Researchers":[105],"potentially":[107],"large":[110],"library":[112],"in":[113,124],"two":[114],"three":[116],"days,":[117],"with":[118],"minimal":[119],"effort":[120],"prior":[122],"experience":[123],"recombinant":[125],"techniques.":[127]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
