{"id":"https://openalex.org/W2410449656","doi":null,"title":"In vitro methods in the study of reactive drug metabolites with liquid chromatography / mass spectrometry","display_name":"In vitro methods in the study of reactive drug metabolites with liquid chromatography / mass spectrometry","publication_year":2016,"publication_date":"2016-01-01","ids":{"openalex":"https://openalex.org/W2410449656","mag":"2410449656"},"language":"en","primary_location":{"id":"mag:2410449656","doi":null,"is_oa":false,"landing_page_url":"http://jultika.oulu.fi/files/isbn9789526212197.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5069750147","display_name":"Toni Lassila","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Toni Lassila","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5069750147"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2434,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.51916239,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.7070000171661377,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.7070000171661377,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11465","display_name":"Drug-Induced Hepatotoxicity and Protection","score":0.20739999413490295,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11117","display_name":"Drug-Induced Adverse Reactions","score":0.01759999990463257,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reactive-drug-metabolites","display_name":"reactive drug metabolites","score":0.9639999866485596},{"id":"https://openalex.org/keywords/idiosyncratic-adverse-drug-reactions","display_name":"idiosyncratic drug reactions","score":0.9729999899864197},{"id":"https://openalex.org/keywords/glutathione-conjugates","display_name":"glutathione conjugates","score":0.9810000061988831},{"id":"https://openalex.org/keywords/pulegone","display_name":"pulegone","score":0.6639999747276306},{"id":"https://openalex.org/keywords/menthofuran","display_name":"menthofuran","score":0.996999979019165},{"id":"https://openalex.org/keywords/acyl-glucuronides","display_name":"acyl glucuronides","score":0.8130000233650208},{"id":"https://openalex.org/keywords/acyl-migration","display_name":"acyl migration","score":0.7360000014305115},{"id":"https://openalex.org/keywords/liver-s9-fraction","display_name":"liver S9 fraction","score":0.8450000286102295},{"id":"https://openalex.org/keywords/subcellular-fractions","display_name":"subcellular fractions","score":0.8180000185966492},{"id":"https://openalex.org/keywords/hepatocytes","display_name":"hepatocytes","score":0.8629999756813049},{"id":"https://openalex.org/keywords/in-vitro-screening","display_name":"in vitro screening","score":0.48399999737739563}],"concepts":[{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.896436870098114},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8396024703979492},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.8047389984130859},{"id":"https://openalex.org/C192937433","wikidata":"https://www.wikidata.org/wiki/Q2134627","display_name":"Reactive intermediate","level":3,"score":0.537370502948761},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5346544981002808},{"id":"https://openalex.org/C89311334","wikidata":"https://www.wikidata.org/wiki/Q1124842","display_name":"Drug metabolism","level":3,"score":0.5338239669799805},{"id":"https://openalex.org/C64489805","wikidata":"https://www.wikidata.org/wiki/Q873864","display_name":"Liquid chromatography\u2013mass spectrometry","level":3,"score":0.4336961507797241},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39527982473373413},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.38935425877571106},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.28662025928497314},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1313340663909912},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2410449656","doi":null,"is_oa":false,"landing_page_url":"http://jultika.oulu.fi/files/isbn9789526212197.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":210,"referenced_works":["https://openalex.org/W119374870","https://openalex.org/W1507082667","https://openalex.org/W1541205806","https://openalex.org/W1547280243","https://openalex.org/W1564948674","https://openalex.org/W1576219812","https://openalex.org/W1601460056","https://openalex.org/W1604080687","https://openalex.org/W1785708403","https://openalex.org/W1845962002","https://openalex.org/W1855439196","https://openalex.org/W1856603289","https://openalex.org/W1868880553","https://openalex.org/W1895993106","https://openalex.org/W1914311513","https://openalex.org/W1942189383","https://openalex.org/W1966746955","https://openalex.org/W1968814270","https://openalex.org/W1969449194","https://openalex.org/W1969780974","https://openalex.org/W1973426823","https://openalex.org/W1974534288","https://openalex.org/W1975290431","https://openalex.org/W1978216187","https://openalex.org/W1978796264","https://openalex.org/W1978896527","https://openalex.org/W1979167419","https://openalex.org/W1980455299","https://openalex.org/W1982396311","https://openalex.org/W1987118114","https://openalex.org/W1987144475","https://openalex.org/W1988801414","https://openalex.org/W1989455369","https://openalex.org/W1989536406","https://openalex.org/W1990174660","https://openalex.org/W1990355599","https://openalex.org/W1991720659","https://openalex.org/W1992073724","https://openalex.org/W1992473667","https://openalex.org/W1992475241","https://openalex.org/W1992646986","https://openalex.org/W1996799408","https://openalex.org/W1996935441","https://openalex.org/W1997486990","https://openalex.org/W1999365897","https://openalex.org/W1999587831","https://openalex.org/W1999881624","https://openalex.org/W2001015949","https://openalex.org/W2003181581","https://openalex.org/W2003831777","https://openalex.org/W2005605345","https://openalex.org/W2005877813","https://openalex.org/W2008129676","https://openalex.org/W2008262185","https://openalex.org/W2008932274","https://openalex.org/W2009497115","https://openalex.org/W2010326342","https://openalex.org/W2013695388","https://openalex.org/W2013735700","https://openalex.org/W2014239314","https://openalex.org/W2014570527","https://openalex.org/W2014843937","https://openalex.org/W2017060911","https://openalex.org/W2018048758","https://openalex.org/W2020583848","https://openalex.org/W2023922443","https://openalex.org/W2024913341","https://openalex.org/W2025113832","https://openalex.org/W2025645149","https://openalex.org/W2027107189","https://openalex.org/W2028005470","https://openalex.org/W2028470385","https://openalex.org/W2028628438","https://openalex.org/W2028825845","https://openalex.org/W2028908020","https://openalex.org/W2030526585","https://openalex.org/W2031349773","https://openalex.org/W2033545087","https://openalex.org/W2033935070","https://openalex.org/W2034400329","https://openalex.org/W2035091931","https://openalex.org/W2036915173","https://openalex.org/W2041431528","https://openalex.org/W2041480771","https://openalex.org/W2041947771","https://openalex.org/W2042064755","https://openalex.org/W2042904782","https://openalex.org/W2043169601","https://openalex.org/W2043676765","https://openalex.org/W2045585044","https://openalex.org/W2045786384","https://openalex.org/W2046823773","https://openalex.org/W2049248673","https://openalex.org/W2050942102","https://openalex.org/W2051809150","https://openalex.org/W2051939729","https://openalex.org/W2052717359","https://openalex.org/W2053010450","https://openalex.org/W2054073416","https://openalex.org/W2057665005","https://openalex.org/W2058656471","https://openalex.org/W2059183805","https://openalex.org/W2060245731","https://openalex.org/W2060869897","https://openalex.org/W2062793968","https://openalex.org/W2062934575","https://openalex.org/W2064734109","https://openalex.org/W2065108839","https://openalex.org/W2066568325","https://openalex.org/W2066664962","https://openalex.org/W2067543062","https://openalex.org/W2067961563","https://openalex.org/W2068150904","https://openalex.org/W2072010935","https://openalex.org/W2073224154","https://openalex.org/W2076022981","https://openalex.org/W2076349503","https://openalex.org/W2077705361","https://openalex.org/W2078470673","https://openalex.org/W2080059382","https://openalex.org/W2080116174","https://openalex.org/W2080843499","https://openalex.org/W2082901471","https://openalex.org/W2083313602","https://openalex.org/W2083635098","https://openalex.org/W2086054530","https://openalex.org/W2087395652","https://openalex.org/W2088218513","https://openalex.org/W2089338956","https://openalex.org/W2092413318","https://openalex.org/W2093411723","https://openalex.org/W2093778527","https://openalex.org/W2094970394","https://openalex.org/W2099220871","https://openalex.org/W2100742272","https://openalex.org/W2101016189","https://openalex.org/W2102800019","https://openalex.org/W2106195907","https://openalex.org/W2106480379","https://openalex.org/W2107421255","https://openalex.org/W2107947237","https://openalex.org/W2108204857","https://openalex.org/W2109521216","https://openalex.org/W2109634874","https://openalex.org/W2110568053","https://openalex.org/W2113038192","https://openalex.org/W2115763439","https://openalex.org/W2116459155","https://openalex.org/W2117481801","https://openalex.org/W2117502107","https://openalex.org/W2119678057","https://openalex.org/W2120568649","https://openalex.org/W2120917180","https://openalex.org/W2121074897","https://openalex.org/W2124289434","https://openalex.org/W2124428389","https://openalex.org/W2125655950","https://openalex.org/W2125768955","https://openalex.org/W2126543731","https://openalex.org/W2127186002","https://openalex.org/W2131072190","https://openalex.org/W2132311212","https://openalex.org/W2132804094","https://openalex.org/W2139054732","https://openalex.org/W2139798209","https://openalex.org/W2140055542","https://openalex.org/W2140306716","https://openalex.org/W2141969112","https://openalex.org/W2142004616","https://openalex.org/W2142148705","https://openalex.org/W2145651065","https://openalex.org/W2146402650","https://openalex.org/W2148906548","https://openalex.org/W2149746810","https://openalex.org/W2150096848","https://openalex.org/W2151975707","https://openalex.org/W2152657460","https://openalex.org/W2156934457","https://openalex.org/W2158094331","https://openalex.org/W2158847414","https://openalex.org/W2161643992","https://openalex.org/W2167202217","https://openalex.org/W2167832449","https://openalex.org/W2168336351","https://openalex.org/W2169652569","https://openalex.org/W2171263500","https://openalex.org/W2173347944","https://openalex.org/W2232936992","https://openalex.org/W2234573628","https://openalex.org/W2234847249","https://openalex.org/W2236237967","https://openalex.org/W2259841021","https://openalex.org/W2268182634","https://openalex.org/W2273388269","https://openalex.org/W2279010485","https://openalex.org/W2314477737","https://openalex.org/W2319811175","https://openalex.org/W2322012164","https://openalex.org/W2324507000","https://openalex.org/W2328974510","https://openalex.org/W2331036577","https://openalex.org/W2344225884","https://openalex.org/W2346211772","https://openalex.org/W2400989676","https://openalex.org/W2404736574","https://openalex.org/W2405140139","https://openalex.org/W2411544551","https://openalex.org/W2465648427","https://openalex.org/W2491892676","https://openalex.org/W2951360964"],"related_works":["https://openalex.org/W2580450538","https://openalex.org/W2160584998","https://openalex.org/W2033545087","https://openalex.org/W2316007211","https://openalex.org/W3022635171","https://openalex.org/W2028908020","https://openalex.org/W1991720659","https://openalex.org/W2281151828","https://openalex.org/W2106927739","https://openalex.org/W2359271864"],"abstract_inverted_index":{"Reactive":[0,50],"metabolites":[1,51,97,129,141,161,184,231,238],"are":[2,237],"believed":[3],"to":[4,19,34,132,155,163,264,274,304],"be":[5,53,73,246,272,283,305],"responsible":[6],"for":[7,38,90,102,152,218],"rare":[8],"but":[9,179],"serious":[10,45],"idiosyncratic":[11],"adverse":[12],"drug":[13,48,263],"reactions":[14,71],"(IADRs)":[15],"that":[16,119,281,310],"have":[17],"led":[18],"the":[20,26,39,91,133,183,206,219,254,259],"withdrawal":[21],"of":[22,95,106,126,159,182,194,197,209,229,232,261,293],"numerous":[23],"drugs":[24,309],"from":[25,136,240,300],"market.":[27],"This":[28],"has":[29],"resulted":[30],"in":[31,47,69,87,112,144,170,216],"major":[32],"harm":[33],"patients,":[35],"economic":[36],"losses":[37],"pharmaceutical":[40],"companies":[41],"and":[42,93,196,205,244,258,296],"represents":[43],"a":[44,262],"problem":[46],"development.":[49],"can":[52,72,245,270],"studied":[54],"by":[55,186],"trapping":[56],"them":[57,301],"with":[58,63,75,176,203,308],"suitable":[59],"nucleophiles,":[60],"most":[61],"commonly":[62,110],"glutathione.":[64],"The":[65,100,191,267,291],"glutathione":[66,192,212],"conjugates":[67,193,280,295,298],"formed":[68,299],"these":[70],"analyzed":[74,202],"liquid":[76],"chromatography":[77],"mass":[78],"spectrometry":[79],"(LC/":[80],"MS)":[81],"techniques.":[82],"In":[83],"this":[84],"study,":[85],"new":[86],"vitro":[88,113],"methods":[89],"detection":[92],"analysis":[94],"reactive":[96,103,128,171,230,285],"were":[98,114,142,150,189,201,214],"developed.":[99],"suitability":[101],"metabolite":[104,172,199],"screening":[105,228],"different":[107,140],"enzyme":[108],"sources":[109],"used":[111],"compared.":[115],"It":[116],"was":[117,174,251,302],"found":[118,252,303],"sub-cellular":[120],"fractions":[121],"yielded":[122],"significantly":[123],"larger":[124,157],"amounts":[125,134,158],"glutathione-trapped":[127,160],"as":[130],"compared":[131],"obtained":[135],"intact":[137],"hepatocytes.":[138],"Additionally,":[139],"detected":[143],"some":[145],"cases.":[146],"Biomimetic":[147],"metalloporphyrin":[148],"catalysts":[149],"tested":[151],"their":[153,287],"ability":[154],"produce":[156],"relative":[162],"liver":[164,187],"S9":[165,188],"fraction":[166],"incubations.":[167],"An":[168],"increase":[169],"production":[173],"observed":[175],"biomimetic":[177],"models,":[178],"not":[180],"all":[181],"produced":[185,239],"observed.":[190],"pulegone":[195],"its":[198],"menthofuran":[200],"LC/MS/MS,":[204],"fragmentation":[207],"spectra":[208],"Nand":[210],"S-/Ndi-linked":[211],"conjugate":[213],"interpreted":[215],"detail":[217],"first":[220],"time.":[221],"These":[222],"results":[223],"will":[224],"enable":[225],"more":[226,284,306],"efficient":[227],"furan-containing":[233],"compounds.":[234],"Acyl":[235],"glucuronides":[236],"carboxylic":[241,268],"acid-containing":[242],"compounds":[243],"reactive.":[247],"A":[248,278],"good":[249],"correlation":[250],"between":[253],"acyl":[255,276,289],"migration":[256],"half-life":[257],"tendency":[260],"cause":[265,311],"IADRs.":[266,312],"moiety":[269],"also":[271],"metabolized":[273],"yield":[275],"coenzyme":[277],"(CoA)":[279],"may":[282],"than":[286],"corresponding":[288],"glucuronides.":[290],"formation":[292],"CoA":[294],"additional":[297],"likely":[307]},"counts_by_year":[{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
