{"id":"https://openalex.org/W2410157558","doi":null,"title":"Simple and rapid purification of F1-ATPase from bovine heart mitochondria by affinity chromatography.","display_name":"Simple and rapid purification of F1-ATPase from bovine heart mitochondria by affinity chromatography.","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W2410157558","mag":"2410157558","pmid":"https://pubmed.ncbi.nlm.nih.gov/1830476"},"language":"en","primary_location":{"id":"pmid:1830476","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1830476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedica biochimica acta","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081979047","display_name":"Berhanu Temesgen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"B Temesgen","raw_affiliation_strings":["Institute of Biochemistry, Karl Marx University, Leipzig, FRG"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biochemistry, Karl Marx University, Leipzig, FRG","institution_ids":["https://openalex.org/I926574661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086851253","display_name":"Klaus Eschrich","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Eschrich","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110385740","display_name":"Eberhard Hofmann","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Hofmann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"50","issue":"1","first_page":"17","last_page":"23"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/f1-atpase","display_name":"F1-ATPase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/bovine-heart-mitochondria","display_name":"bovine heart mitochondria","score":0.8450000286102295},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.746999979019165},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.9390000104904175},{"id":"https://openalex.org/keywords/polyethylene-glycol-precipitation","display_name":"polyethylene glycol precipitation","score":0.9449999928474426},{"id":"https://openalex.org/keywords/atp-hydrolysis-kinetics","display_name":"ATP hydrolysis kinetics","score":0.6690000295639038},{"id":"https://openalex.org/keywords/adp-inhibition","display_name":"ADP inhibition","score":0.8980000019073486},{"id":"https://openalex.org/keywords/mitochondrial-atpase","display_name":"mitochondrial ATPase","score":0.5860000252723694}],"concepts":[{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.749362587928772},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.729077160358429},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6711576581001282},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.6005176305770874},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5169100761413574},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.49654847383499146},{"id":"https://openalex.org/C132946820","wikidata":"https://www.wikidata.org/wiki/Q2509459","display_name":"Differential centrifugation","level":2,"score":0.45285019278526306},{"id":"https://openalex.org/C2776964284","wikidata":"https://www.wikidata.org/wiki/Q410083","display_name":"Polyethylene glycol","level":2,"score":0.44851285219192505},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.4455889165401459},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.42077505588531494},{"id":"https://openalex.org/C2781144210","wikidata":"https://www.wikidata.org/wiki/Q172275","display_name":"Chloroform","level":2,"score":0.41219601035118103},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3874175548553467}],"mesh":[{"descriptor_ui":"D000244","descriptor_name":"Adenosine Diphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004396","descriptor_name":"Coloring Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006180","descriptor_name":"Proton-Translocating ATPases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006180","descriptor_name":"Proton-Translocating ATPases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008929","descriptor_name":"Mitochondria, Heart","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D014227","descriptor_name":"Triazines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015636","descriptor_name":"Magnesium Chloride","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:1830476","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1830476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedica biochimica acta","raw_type":"Journal Article"},{"id":"mag:2410157558","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1830476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W997441242","https://openalex.org/W1979493659","https://openalex.org/W2006650010","https://openalex.org/W2061036502","https://openalex.org/W1880913055","https://openalex.org/W1477210374","https://openalex.org/W1512390315","https://openalex.org/W2005662376","https://openalex.org/W2009725487","https://openalex.org/W1621644718"],"abstract_inverted_index":{"A":[0],"simple":[1],"and":[2,23,88],"rapid":[3],"method":[4],"for":[5],"the":[6,37,55,65,72,93],"isolation":[7],"of":[8,36,57,67,71,95,98],"bovine":[9],"heart":[10],"mitochondrial":[11],"adenosine":[12],"5'-triphosphatase":[13],"(F1-ATPase)":[14],"was":[15,29,39,60,74],"developed.":[16],"Mitochondria":[17],"were":[18],"purified":[19,80],"by":[20,31,41,46,62,76],"differential":[21],"centrifugation":[22],"stored":[24],"frozen.":[25],"After":[26],"thawing.":[27],"F1-ATPase":[28,59,81],"released":[30],"treatment":[32],"with":[33],"chloroform.":[34],"Purification":[35],"enzyme":[38,73],"achieved":[40],"polyethylene":[42],"glycol":[43],"precipitation":[44],"followed":[45],"chromatography":[47],"on":[48],"Procion":[49],"Navy":[50],"H-ER":[51],"beaded":[52],"cellulose":[53],"in":[54,64,92],"presence":[56,94],"MgCl2.":[58,68],"eluted":[61],"ATP":[63,87],"absence":[66],"The":[69,79],"purity":[70],"proved":[75],"SDS-polyacrylamide-gel":[77],"electrophoresis.":[78],"showed":[82],"slightly":[83],"non-hyperbolic":[84],"kinetics":[85],"towards":[86],"nearly":[89],"complete":[90],"inhibition":[91],"millimolar":[96],"concentrations":[97],"ADP.":[99]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}