{"id":"https://openalex.org/W2409686331","doi":null,"title":"Using sodium chloride step gradients to fractionate DNA fragments.","display_name":"Using sodium chloride step gradients to fractionate DNA fragments.","publication_year":1991,"publication_date":"1991-04-01","ids":{"openalex":"https://openalex.org/W2409686331","mag":"2409686331","pmid":"https://pubmed.ncbi.nlm.nih.gov/1907833"},"language":"en","primary_location":{"id":"pmid:1907833","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1907833","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108806972","display_name":"Pamela S. Fink","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I19648265","display_name":"Wright State University","ror":"https://ror.org/04qk6pt94","country_code":"US","type":"education","lineage":["https://openalex.org/I19648265"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"P S Fink","raw_affiliation_strings":["Department of Biological Sciences, Wright State University, Dayton, OH 45435"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Wright State University, Dayton, OH 45435","institution_ids":["https://openalex.org/I19648265"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5108806972"],"corresponding_institution_ids":["https://openalex.org/I19648265"],"apc_list":null,"apc_paid":null,"fwci":0.1113,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.37812915,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":"10","issue":"4","first_page":"446, 448, 450","last_page":"446, 448, 450"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6455000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6455000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.13230000436306,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.052299998700618744,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-fractionation","display_name":"DNA fractionation","score":0.7940000295639038},{"id":"https://openalex.org/keywords/ultracentrifugation","display_name":"ultracentrifugation","score":0.9710000157356262},{"id":"https://openalex.org/keywords/bacteriophage-lambda","display_name":"bacteriophage lambda","score":0.7720000147819519},{"id":"https://openalex.org/keywords/chromosomal-dna","display_name":"chromosomal DNA","score":0.7509999871253967},{"id":"https://openalex.org/keywords/dna-ligase","display_name":"DNA ligase","score":0.4350000023841858},{"id":"https://openalex.org/keywords/ethanol-precipitation","display_name":"ethanol precipitation","score":0.7940000295639038},{"id":"https://openalex.org/keywords/southern-blot","display_name":"Southern blot","score":0.6389999985694885},{"id":"https://openalex.org/keywords/dna-cloning","display_name":"DNA cloning","score":0.36800000071525574}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.72270667552948},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6067808866500854},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.6033698320388794},{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.5822203159332275},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.5444477796554565},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5394608974456787},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5254315733909607},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.519291341304779},{"id":"https://openalex.org/C53941386","wikidata":"https://www.wikidata.org/wiki/Q2571416","display_name":"Ethanol precipitation","level":3,"score":0.49614962935447693},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.4799945056438446},{"id":"https://openalex.org/C54689828","wikidata":"https://www.wikidata.org/wiki/Q2029259","display_name":"Ultracentrifuge","level":2,"score":0.47895151376724243},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.4698293209075928},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.4476791024208069},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.43429893255233765},{"id":"https://openalex.org/C2889275","wikidata":"https://www.wikidata.org/wiki/Q7316313","display_name":"Restriction digest","level":4,"score":0.4335031807422638},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.35348355770111084},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27327960729599},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.2145146131515503},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.1851460337638855},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07357513904571533},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D002499","descriptor_name":"Centrifugation, Density Gradient","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012965","descriptor_name":"Sodium Chloride","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015139","descriptor_name":"Blotting, Southern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:1907833","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1907833","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"mag:2409686331","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1907833","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2033201878","https://openalex.org/W1989841735","https://openalex.org/W2081251577","https://openalex.org/W1977074883","https://openalex.org/W1976044744","https://openalex.org/W2395799778","https://openalex.org/W2409018335","https://openalex.org/W2057878200","https://openalex.org/W1964624903","https://openalex.org/W2014532476"],"abstract_inverted_index":{"A":[0,52],"method":[1,70],"is":[2],"described":[3],"for":[4],"the":[5],"separation":[6],"of":[7,34],"DNA":[8,35,77],"fragments":[9,62,79],"by":[10,40,47,57],"ultracentrifugation":[11],"through":[12],"a":[13,27],"sodium":[14],"chloride":[15],"step":[16],"gradient.":[17],"The":[18,69],"gradients":[19],"are":[20],"quickly":[21],"and":[22,25,50],"easily":[23],"prepared":[24],"require":[26],"five-":[28],"to":[29,66,74],"six-hour":[30],"centrifugation.":[31],"Fractionated":[32],"samples":[33],"may":[36],"be":[37],"directly":[38],"examined":[39],"agarose":[41],"gel":[42],"electrophoresis,":[43],"then":[44],"further":[45],"analyzed":[46],"Southern":[48],"transfer":[49],"hybridization.":[51],"simple":[53],"ethanol":[54,59],"precipitation":[55],"followed":[56],"several":[58],"washes":[60],"yields":[61],"that":[63],"ligate":[64],"efficiently":[65],"vector":[67],"DNA.":[68,88],"has":[71],"been":[72],"applied":[73],"separate":[75],"chromosomal":[76],"restriction":[78],"as":[80,82],"well":[81],"bacteriophage":[83],"lambda":[84],"arms":[85],"from":[86],"insert":[87]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
