{"id":"https://openalex.org/W2409255285","doi":null,"title":"Antisense IRAK-2 oligodeoxynucleotide inhibits interleukin-1-induced nuclear factor-kappa B activation in vitro.","display_name":"Antisense IRAK-2 oligodeoxynucleotide inhibits interleukin-1-induced nuclear factor-kappa B activation in vitro.","publication_year":2000,"publication_date":"2000-04-01","ids":{"openalex":"https://openalex.org/W2409255285","mag":"2409255285","pmid":"https://pubmed.ncbi.nlm.nih.gov/11324458"},"language":"en","primary_location":{"id":"pmid:11324458","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11324458","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta pharmacologica Sinica","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001430135","display_name":"Fukun Guo","orcid":"https://orcid.org/0000-0002-8820-7633","observed_orcids":["https://orcid.org/0000-0002-8820-7633"]},"institutions":[{"id":"https://openalex.org/I58200834","display_name":"Southern Medical University","ror":"https://ror.org/01vjw4z39","country_code":"CN","type":"education","lineage":["https://openalex.org/I58200834"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"F K Guo","raw_affiliation_strings":["Institute of Pharmaceutic Sciences, First Military Medical University, Guangzhou 510515, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Pharmaceutic Sciences, First Military Medical University, Guangzhou 510515, China","institution_ids":["https://openalex.org/I58200834"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101743351","display_name":"Yilei Li","orcid":"https://orcid.org/0000-0002-0964-5695","observed_orcids":["https://orcid.org/0000-0002-0964-5695","https://orcid.org/0000-0002-0289-9510"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Y L Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5100513876","display_name":"Shuguang Wu","orcid":"https://orcid.org/0009-0005-8180-184X","observed_orcids":["https://orcid.org/0009-0005-8180-184X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S G Wu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1291,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.49556568,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"21","issue":"4","first_page":"318","last_page":"20"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11591","display_name":"NF-\u03baB Signaling Pathways","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11591","display_name":"NF-\u03baB Signaling Pathways","score":0.9937000274658203,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13026","display_name":"Natural Compounds in Disease Treatment","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antisense-oligodeoxynucleotides","display_name":"antisense oligodeoxynucleotides","score":0.9860000014305115},{"id":"https://openalex.org/keywords/interleukin-1","display_name":"interleukin-1","score":0.9760000109672546},{"id":"https://openalex.org/keywords/nf-kappab","display_name":"NF-\u03baB","score":0.7319999933242798},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.6710000038146973},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.8759999871253967}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6990168690681458},{"id":"https://openalex.org/C2778724333","wikidata":"https://www.wikidata.org/wiki/Q14401","display_name":"Kappa","level":2,"score":0.5577815771102905},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5423617362976074},{"id":"https://openalex.org/C123298856","wikidata":"https://www.wikidata.org/wiki/Q15317427","display_name":"NFKB1","level":4,"score":0.5221824645996094},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.46563780307769775},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.40834173560142517},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40528160333633423},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.33730393648147583},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18509280681610107},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.150655597448349},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12198615074157715},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004622","descriptor_name":"Embryo, Mammalian","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007375","descriptor_name":"Interleukin-1","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D007375","descriptor_name":"Interleukin-1","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D007375","descriptor_name":"Interleukin-1","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D011494","descriptor_name":"Protein Kinases","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016328","descriptor_name":"NF-kappa B","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020319","descriptor_name":"Oligodeoxyribonucleotides, Antisense","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020319","descriptor_name":"Oligodeoxyribonucleotides, Antisense","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D020319","descriptor_name":"Oligodeoxyribonucleotides, Antisense","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D053592","descriptor_name":"Interleukin-1 Receptor-Associated Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053592","descriptor_name":"Interleukin-1 Receptor-Associated Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053592","descriptor_name":"Interleukin-1 Receptor-Associated Kinases","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:11324458","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11324458","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta pharmacologica Sinica","raw_type":"Journal Article"},{"id":"mag:2409255285","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11324458","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":null,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.5400000214576721,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2923619641","https://openalex.org/W2051914105","https://openalex.org/W1983243357","https://openalex.org/W111969745","https://openalex.org/W2011957276","https://openalex.org/W4243212462","https://openalex.org/W1920787626","https://openalex.org/W1986437487","https://openalex.org/W2302005959","https://openalex.org/W2356655692"],"abstract_inverted_index":{"AIM:":[0],"To":[1],"study":[2],"the":[3],"inhibitory":[4],"effects":[5],"of":[6,44,55],"antisense":[7,109],"interleukin-1":[8,16],"receptor":[9],"associated":[10],"kinase-2":[11],"(IRAK-2)":[12],"oligodeoxynucleotide":[13],"(ODN)":[14],"on":[15],"(IL-1)-stimulated":[17],"nuclear":[18],"factor-kappa":[19],"B":[20,46,63,78,124],"(NF-kappa":[21],"B)":[22],"activation.":[23,125],"METHODS:":[24],"Antisense":[25,72,118],"IRAK-2":[26,73,110,119],"ODN":[27,74,111,120],"was":[28,93],"delivered":[29],"by":[30,49,67],"lipofectin":[31],"encapsulation":[32],"into":[33],"human":[34],"embryonic":[35],"kidney":[36],"293":[37,40,57],"cells":[38,58],"(HEK":[39],"cells).":[41],"The":[42],"levels":[43],"NF-kappa":[45,62,77,123],"were":[47],"assayed":[48],"sandwich":[50],"ELISA.":[51],"RESULTS:":[52],"(1)":[53],"Treatment":[54],"HEK":[56],"with":[59,108],"IL-1":[60],"enhanced":[61],"level":[64],"in":[65,80],"nuclei":[66],"518.5%":[68],"+/-":[69,95,100,104],"2.1%.":[70],"(2)":[71],"inhibited":[75,121],"IL-1-induced":[76,122],"activation":[79],"a":[81],"concentration":[82],"(1-8":[83],"micrograms)-":[84],"and":[85],"time":[86],"(5-24":[87],"h)-dependent":[88],"manner.":[89],"A":[90],"maximum":[91],"inhibition":[92],"70.7%":[94],"1.0%":[96],"from":[97],"Acontrol":[98],"0.834":[99],"0.014":[101],"to":[102],"0.244":[103],"0.008":[105],"after":[106],"treatment":[107],"4":[112],"micrograms":[113],"for":[114],"8":[115],"h.":[116],"CONCLUSION:":[117]},"counts_by_year":[],"updated_date":"2026-09-24T07:57:37.273135","created_date":"2025-10-10T00:00:00"}