{"id":"https://openalex.org/W2409024154","doi":"https://doi.org/10.1055/s-0038-1646564","title":"Methylene Blue Enhances Lateral Association of Fibrin Resulting in Rapid Gelation and Thick Fiber Formation","display_name":"Methylene Blue Enhances Lateral Association of Fibrin Resulting in Rapid Gelation and Thick Fiber Formation","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W2409024154","doi":"https://doi.org/10.1055/s-0038-1646564","mag":"2409024154","pmid":"https://pubmed.ncbi.nlm.nih.gov/2749596"},"language":"en","primary_location":{"id":"doi:10.1055/s-0038-1646564","doi":"https://doi.org/10.1055/s-0038-1646564","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1646564","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111713733","display_name":"Marcus E. Carr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801770128","display_name":"Hunter Holmes McGuire VA Medical Center","ror":"https://ror.org/04fp78s33","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I2801770128","https://openalex.org/I4210159720"]},{"id":"https://openalex.org/I72019824","display_name":"Virginia Commonwealth University Medical Center","ror":"https://ror.org/057xmsr27","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I72019824"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcus E Carr","raw_affiliation_strings":["The Division of Hematology/Oncology and Coagulation Special Studies Laboratory, Departments of Medicine and Pathology, Medical College of Virginia, USA, and McGuire V. A. Medical Center, Richmond, Mrginia, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Division of Hematology/Oncology and Coagulation Special Studies Laboratory, Departments of Medicine and Pathology, Medical College of Virginia, USA, and McGuire V. A. Medical Center, Richmond, Mrginia, USA","institution_ids":["https://openalex.org/I2801770128","https://openalex.org/I72019824"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5049560979","display_name":"Patrick L Powers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801770128","display_name":"Hunter Holmes McGuire VA Medical Center","ror":"https://ror.org/04fp78s33","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I2801770128","https://openalex.org/I4210159720"]},{"id":"https://openalex.org/I72019824","display_name":"Virginia Commonwealth University Medical Center","ror":"https://ror.org/057xmsr27","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I72019824"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Patrick L Powers","raw_affiliation_strings":["The Division of Hematology/Oncology and Coagulation Special Studies Laboratory, Departments of Medicine and Pathology, Medical College of Virginia, USA, and McGuire V. A. Medical Center, Richmond, Mrginia, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Division of Hematology/Oncology and Coagulation Special Studies Laboratory, Departments of Medicine and Pathology, Medical College of Virginia, USA, and McGuire V. A. Medical Center, Richmond, Mrginia, USA","institution_ids":["https://openalex.org/I2801770128","https://openalex.org/I72019824"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0695,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.80746982,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"61","issue":"02","first_page":"230","last_page":"233"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11721","display_name":"Blood properties and coagulation","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12548","display_name":"Hemostasis and retained surgical items","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/methylene-blue","display_name":"methylene blue","score":1.0},{"id":"https://openalex.org/keywords/fibrinogen-precipitation","display_name":"fibrinogen precipitation","score":0.8090000152587891},{"id":"https://openalex.org/keywords/thrombin-time","display_name":"thrombin time","score":0.8069999814033508},{"id":"https://openalex.org/keywords/calcium-chloride","display_name":"calcium chloride","score":0.6539999842643738},{"id":"https://openalex.org/keywords/heparin-reversal","display_name":"heparin reversal","score":0.8090000152587891}],"concepts":[{"id":"https://openalex.org/C2779036427","wikidata":"https://www.wikidata.org/wiki/Q381899","display_name":"Fibrinogen","level":2,"score":0.8796699047088623},{"id":"https://openalex.org/C54173615","wikidata":"https://www.wikidata.org/wiki/Q27205","display_name":"Fibrin","level":2,"score":0.8617634177207947},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7434167861938477},{"id":"https://openalex.org/C2779191827","wikidata":"https://www.wikidata.org/wiki/Q422134","display_name":"Methylene blue","level":4,"score":0.6704761981964111},{"id":"https://openalex.org/C2777292125","wikidata":"https://www.wikidata.org/wiki/Q409166","display_name":"Thrombin","level":3,"score":0.6109448075294495},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5594550371170044},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.4315549433231354},{"id":"https://openalex.org/C77171857","wikidata":"https://www.wikidata.org/wiki/Q5135593","display_name":"Clotting time","level":3,"score":0.4135858714580536},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3412182033061981},{"id":"https://openalex.org/C2778382381","wikidata":"https://www.wikidata.org/wiki/Q179217","display_name":"Coagulation","level":2,"score":0.3201000690460205},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2199764847755432},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.219955712556839},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.131477952003479},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.12836411595344543},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09803664684295654},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.09538871049880981},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0},{"id":"https://openalex.org/C65165184","wikidata":"https://www.wikidata.org/wiki/Q218831","display_name":"Photocatalysis","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D005337","descriptor_name":"Fibrin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005340","descriptor_name":"Fibrinogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005782","descriptor_name":"Gels","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008751","descriptor_name":"Methylene Blue","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D013918","descriptor_name":"Thrombin Time","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1055/s-0038-1646564","doi":"https://doi.org/10.1055/s-0038-1646564","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1646564","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"journal-article"},{"id":"pmid:2749596","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2749596","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and haemostasis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2090302168","https://openalex.org/W1998099489","https://openalex.org/W2141647945","https://openalex.org/W1966496341","https://openalex.org/W4231275415","https://openalex.org/W3161827037","https://openalex.org/W2018603472","https://openalex.org/W2010311038","https://openalex.org/W2055658056","https://openalex.org/W1975959426"],"abstract_inverted_index":{"Methylene":[0],"blue":[1],"(MB)":[2],"has":[3],"been":[4],"suggested":[5],"as":[6,139],"a":[7],"therapeutic":[8],"alternative":[9],"for":[10,88],"heparin":[11],"reversal":[12],"in":[13,78,124,136,208,220],"patients":[14],"sensitive":[15],"to":[16,62,93,102,119,131,146,150,171,201,234],"protamine.":[17],"We":[18],"investigated":[19],"the":[20,25,40,74,86,98,140,151,186,204,209,215],"impact":[21,216],"of":[22,29,45,50,69,76,154,161,217],"MB":[23,36,63,77,96,141,155,168,176,179,196,218],"on":[24,156],"assembly":[26],"and":[27,33,49,81,84,106,128,203],"structure":[28],"thrombin":[30,41,202],"induced":[31],"fibrin":[32,112,126],"plasma":[34,46,82,137,223],"gels.":[35],"(1,600":[37],"micrograms/ml)":[38],"reduced":[39],"clotting":[42],"time":[43],"(TCT)":[44],"by":[47,53],"30%":[48],"purified":[51,80,90,125],"fibrinogen":[52,65,91,162,184],"46%.":[54],"Above":[55],"1,600":[56],"micrograms/ml,":[57],"TCTs":[58],"were":[59,206],"prolonged":[60],"due":[61],"mediated":[64],"precipitation.":[66],"The":[67,111],"presence":[68],"5":[70],"mM":[71],"CaCl2":[72],"masked":[73],"effect":[75,153],"both":[79],"systems":[83],"lowered":[85],"threshold":[87],"MB-induced":[89],"precipitation":[92],"800":[94],"micrograms/ml.":[95,148],"shortened":[97],"lag":[99],"phase":[100],"prior":[101,200],"thrombin-induced":[103],"turbidity":[104],"increase,":[105],"enhanced":[107],"final":[108],"gel":[109],"turbidity.":[110],"fiber":[113],"mass/length":[114],"ratio":[115],"increased":[116,143],"from":[117,129,144,228],"5.2":[118],"13.1":[120],"X":[121,133],"10(13)":[122,134],"dalton/cm":[123,135],"gels":[127,138],"3.2":[130],"10.4":[132],"concentration":[142],"0":[145],"200":[147],"Due":[149],"photooxidant":[152],"fibrinogen,":[157],"rapid":[158],"time-dependent":[159],"loss":[160],"clottability":[163],"was":[164,197],"obvious":[165],"at":[166],"low":[167],"concentrations":[169],"(50":[170],"400":[172],"micrograms/ml).":[173],"At":[174],"high":[175],"concentrations,":[177],"intense":[178],"light":[180,229],"absorption":[181],"partially":[182],"protected":[183],"within":[185],"sample.":[187],"Accurate":[188],"measurements":[189],"could":[190],"only":[191],"be":[192,226,235],"made,":[193],"however,":[194],"when":[195],"added":[198],"just":[199],"assays":[205],"performed":[207],"dark.":[210],"While":[211],"erythrocytes":[212],"may":[213],"reduce":[214],"photooxidation":[219],"whole":[221],"blood,":[222],"samples":[224],"must":[225],"shielded":[227],"if":[230],"reproducible":[231],"results":[232],"are":[233],"obtained.":[236]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}