{"id":"https://openalex.org/W2408829224","doi":"https://doi.org/10.1007/978-1-59745-277-9_21","title":"Characterization of the Adenovirus Fiber Protein","display_name":"Characterization of the Adenovirus Fiber Protein","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2408829224","doi":"https://doi.org/10.1007/978-1-59745-277-9_21","mag":"2408829224","pmid":"https://pubmed.ncbi.nlm.nih.gov/17656791"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-277-9_21","doi":"https://doi.org/10.1007/978-1-59745-277-9_21","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-277-9_21","pdf_url":null,"source":{"id":"https://openalex.org/S2756129150","display_name":"Methods in molecular medicine","issn_l":"1543-1894","issn":["1543-1894","1940-6037"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Medicine\u2122","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W643405167","display_name":"Adenovirus Methods and Protocols"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017592501","display_name":"Jeffrey A. Engler","orcid":"https://orcid.org/0000-0002-7263-3574","observed_orcids":["https://orcid.org/0000-0002-7263-3574","https://orcid.org/0000-0001-9114-6221"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffrey A. Engler","raw_affiliation_strings":["Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091626287","display_name":"Jeong Shin Hong","orcid":"https://orcid.org/0000-0003-4719-1855","observed_orcids":["https://orcid.org/0000-0003-4719-1855"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeong Shin Hong","raw_affiliation_strings":["Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32389192"],"apc_list":null,"apc_paid":null,"fwci":1.0157,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.73635925,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"131","issue":null,"first_page":"281","last_page":"298"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus-fiber-protein","display_name":"adenovirus fiber protein","score":0.9229999780654907},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.7089999914169312},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.7269999980926514},{"id":"https://openalex.org/keywords/polyclonal-antibodies","display_name":"polyclonal antibodies","score":0.9739999771118164},{"id":"https://openalex.org/keywords/o-glcnac","display_name":"O-GlcNAc","score":0.8830000162124634},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.9269999861717224},{"id":"https://openalex.org/keywords/tissue-tropism","display_name":"tissue tropism","score":0.8190000057220459}],"concepts":[{"id":"https://openalex.org/C202878990","wikidata":"https://www.wikidata.org/wiki/Q830465","display_name":"Capsid","level":3,"score":0.8308969140052795},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.7249491810798645},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.5895130634307861},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.5862066745758057},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.5325851440429688},{"id":"https://openalex.org/C139770010","wikidata":"https://www.wikidata.org/wiki/Q1339807","display_name":"Internalization","level":3,"score":0.5127914547920227},{"id":"https://openalex.org/C35693153","wikidata":"https://www.wikidata.org/wiki/Q239762","display_name":"Tropism","level":3,"score":0.44517266750335693},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.42839688062667847},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.4245827794075012},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4211779236793518},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.4130447208881378},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.39381545782089233},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39042115211486816},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3570246696472168},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3328118920326233},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.24427515268325806},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19951307773590088},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08086755871772766},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07587742805480957}],"mesh":[{"descriptor_ui":"D000256","descriptor_name":"Adenoviridae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006367","descriptor_name":"HeLa Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019084","descriptor_name":"Fluorescent Antibody Technique, Indirect","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-59745-277-9_21","doi":"https://doi.org/10.1007/978-1-59745-277-9_21","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-277-9_21","pdf_url":null,"source":{"id":"https://openalex.org/S2756129150","display_name":"Methods in molecular medicine","issn_l":"1543-1894","issn":["1543-1894","1940-6037"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Medicine\u2122","raw_type":"book-chapter"},{"id":"pmid:17656791","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17656791","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular medicine","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W12481932","https://openalex.org/W31284329","https://openalex.org/W267630806","https://openalex.org/W278358209","https://openalex.org/W1450435416","https://openalex.org/W1482658621","https://openalex.org/W1488139785","https://openalex.org/W1494038226","https://openalex.org/W1500939515","https://openalex.org/W1502058993","https://openalex.org/W1507619429","https://openalex.org/W1561390296","https://openalex.org/W1563488686","https://openalex.org/W1571334377","https://openalex.org/W1589849472","https://openalex.org/W1594450556","https://openalex.org/W1652316604","https://openalex.org/W1757736235","https://openalex.org/W1857650329","https://openalex.org/W1870555175","https://openalex.org/W1907874446","https://openalex.org/W1930355415","https://openalex.org/W1940378630","https://openalex.org/W1940483302","https://openalex.org/W1970826779","https://openalex.org/W1971447141","https://openalex.org/W1991905824","https://openalex.org/W1997559299","https://openalex.org/W2019521487","https://openalex.org/W2019834870","https://openalex.org/W2021850477","https://openalex.org/W2022939759","https://openalex.org/W2027980197","https://openalex.org/W2030867630","https://openalex.org/W2041620045","https://openalex.org/W2045576046","https://openalex.org/W2046579159","https://openalex.org/W2050560857","https://openalex.org/W2051758487","https://openalex.org/W2053140016","https://openalex.org/W2053797451","https://openalex.org/W2063729953","https://openalex.org/W2077317257","https://openalex.org/W2080961187","https://openalex.org/W2084550104","https://openalex.org/W2087586754","https://openalex.org/W2100837269","https://openalex.org/W2116071714","https://openalex.org/W2128375279","https://openalex.org/W2140362030","https://openalex.org/W2161876759","https://openalex.org/W2167454422","https://openalex.org/W2288053228","https://openalex.org/W2748101318"],"related_works":["https://openalex.org/W4211077361","https://openalex.org/W1500939515","https://openalex.org/W2133822846","https://openalex.org/W2040630015","https://openalex.org/W1975370346","https://openalex.org/W6964427679","https://openalex.org/W2149872176","https://openalex.org/W2115862235","https://openalex.org/W2019834870","https://openalex.org/W1928181584"],"abstract_inverted_index":{"Entry":[0],"of":[1,10,20,31,43,62,74,84,92,102,110,128,142,167],"the":[2,7,21,25,32,49,58,63,72,75,93,164],"adenovirus":[3],"(Ad)":[4],"capsids":[5],"during":[6],"early":[8,35],"stages":[9],"infection":[11],"is":[12,120],"a":[13,41,126,132],"multistep":[14],"process":[15],"that":[16,116],"includes":[17],"initial":[18,50],"attachment":[19,51],"virus":[22,33],"capsid":[23],"to":[24],"cell":[26],"surface":[27],"followed":[28],"by":[29,95,99],"internalization":[30],"into":[34],"endosomes.":[36],"The":[37,108],"Ad":[38,64,118,123],"fiber":[39,65,119,124],"protein,":[40,160],"complex":[42],"three":[44],"apparently":[45],"identical":[46],"subunits,":[47],"mediates":[48],"step.":[52],"In":[53],"this":[54,143,159],"chapter,":[55],"methods":[56,70,137],"for":[57,71,86,90,138],"purification":[59],"and":[60,98,113,140,151],"characterization":[61,91,141],"protein":[66,76,85,94],"are":[67,105,145],"presented.":[68,107],"Chromatographic":[69],"isolation":[73],"from":[77,125],"infected":[78,103],"cells":[79,104],"can":[80,154],"yield":[81],"substantial":[82],"quantities":[83],"biochemical":[87],"analysis.":[88],"Protocols":[89],"Western":[96],"blot":[97],"indirect":[100],"immunofluorescence":[101],"also":[106,121,130,146],"specificity":[109],"different":[111],"monoclonal":[112],"polyclonal":[114],"antibodies":[115],"recognize":[117],"discussed.":[122],"number":[127],"serotypes":[129],"contains":[131],"posttranslational":[133],"modification,":[134],"O-linked":[135],"N-acetyl-glucosamine;":[136],"detection":[139],"modification":[144],"provided.":[147],"With":[148],"these":[149],"tools":[150],"protocols,":[152],"one":[153],"address":[155],"important":[156],"questions":[157],"about":[158],"which":[161],"helps":[162],"direct":[163],"tissue":[165],"tropism":[166],"Ad.":[168]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}