{"id":"https://openalex.org/W2408221665","doi":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","title":"Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule","display_name":"Expression of mRNA (D2) encoding a protein involved in amino acid transport in S3 proximal tubule","publication_year":1992,"publication_date":"1992-12-01","ids":{"openalex":"https://openalex.org/W2408221665","doi":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","mag":"2408221665","pmid":"https://pubmed.ncbi.nlm.nih.gov/1481885"},"language":"en","primary_location":{"id":"doi:10.1152/ajprenal.1992.263.6.f1087","doi":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","pdf_url":null,"source":{"id":"https://openalex.org/S200780590","display_name":"American Journal of Physiology-Renal Physiology","issn_l":"1522-1466","issn":["1522-1466","1931-857X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Renal Physiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Y. Kanai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y. Kanai","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054430002","display_name":"Matthias G. Stelzner","orcid":"https://orcid.org/0000-0002-6988-847X","observed_orcids":["https://orcid.org/0000-0002-6988-847X"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. G. Stelzner","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046071625","display_name":"Wonhye Lee","orcid":"https://orcid.org/0000-0002-4203-2209","observed_orcids":["https://orcid.org/0000-0002-4203-2209"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"W. S. Lee","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045288947","display_name":"Rebecca G. Wells","orcid":"https://orcid.org/0000-0002-6988-4102","observed_orcids":["https://orcid.org/0000-0002-6988-4102"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R. G. Wells","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087672907","display_name":"Dennis Brown","orcid":"https://orcid.org/0000-0002-5011-7798","observed_orcids":["https://orcid.org/0000-0002-5011-7798"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"D. Brown","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078842111","display_name":"Matthias A. Hediger","orcid":"https://orcid.org/0000-0003-1946-027X","observed_orcids":["https://orcid.org/0000-0003-1946-027X"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. A. Hediger","raw_affiliation_strings":["Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Brigham and Women's Hospital, Boston,Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1283280774"],"apc_list":null,"apc_paid":null,"fwci":2.2255,"has_fulltext":false,"cited_by_count":64,"citation_normalized_percentile":{"value":0.88874143,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"263","issue":"6","first_page":"F1087","last_page":"F1092"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/amino-acid-transport","display_name":"amino acid transport","score":0.6700000166893005},{"id":"https://openalex.org/keywords/renal-mrna-expression","display_name":"renal mRNA expression","score":0.6349999904632568},{"id":"https://openalex.org/keywords/cystine-uptake","display_name":"cystine uptake","score":0.8190000057220459},{"id":"https://openalex.org/keywords/na-independent-transport","display_name":"Na+-independent transport","score":0.7200000286102295},{"id":"https://openalex.org/keywords/ecto-atpase","display_name":"ecto-ATPase","score":0.8600000143051147},{"id":"https://openalex.org/keywords/rat-kidney","display_name":"rat kidney","score":0.45399999618530273}],"concepts":[{"id":"https://openalex.org/C177707886","wikidata":"https://www.wikidata.org/wiki/Q48520","display_name":"Northern blot","level":4,"score":0.6807737350463867},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6488208174705505},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6353300213813782},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6118581295013428},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.5620535016059875},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5393728017807007},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.4931146800518036},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.47364819049835205},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.44431304931640625},{"id":"https://openalex.org/C2777187973","wikidata":"https://www.wikidata.org/wiki/Q5421703","display_name":"Expression cloning","level":4,"score":0.4248759150505066},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19593536853790283}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007687","descriptor_name":"Kidney Tubules, Proximal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017207","descriptor_name":"Rats, Sprague-Dawley","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajprenal.1992.263.6.f1087","doi":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajprenal.1992.263.6.f1087","pdf_url":null,"source":{"id":"https://openalex.org/S200780590","display_name":"American Journal of Physiology-Renal Physiology","issn_l":"1522-1466","issn":["1522-1466","1931-857X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320261","host_organization_name":"American Physical Society","host_organization_lineage":["https://openalex.org/P4310320261"],"host_organization_lineage_names":["American Physical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Renal Physiology","raw_type":"journal-article"},{"id":"pmid:1481885","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1481885","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3301562563","display_name":"MOLECULAR BIOLOGY OF RENAL GLUCOSE TRANSPORT IN DIABETES","funder_award_id":"R01DK043632","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G3741628446","display_name":null,"funder_award_id":"DK-42956","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G8918286541","display_name":"MOLECULAR BIOLOGY OF BRUSH BORDER TRANSPORT PROTEINS","funder_award_id":"R01DK043171","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2009610912","https://openalex.org/W2046507378","https://openalex.org/W2000620101","https://openalex.org/W1969339813","https://openalex.org/W1495130493","https://openalex.org/W2049962978","https://openalex.org/W2053996538","https://openalex.org/W2400877895","https://openalex.org/W3146624464","https://openalex.org/W1753886663"],"abstract_inverted_index":{"A":[0],"rat":[1,155],"kidney-":[2],"and":[3,14,16,36,98,107,139,169,199],"intestine-specific":[4],"cDNA":[5],"(D2)":[6],"that":[7,111,126,147],"induces":[8],"high-affinity,":[9],"Na(+)-independent":[10],"uptake":[11],"of":[12,74,85,103,180,187,194,197,208],"cystine":[13,198],"dibasic":[15],"neutral":[17],"amino":[18,78,201],"acids":[19],"into":[20,71],"cRNA-injected":[21],"Xenopus":[22],"oocytes":[23],"was":[24,92],"recently":[25],"isolated":[26],"by":[27,94,99],"expression":[28,87,186],"cloning":[29],"in":[30,76,88,104,153,176,210],"our":[31],"laboratory":[32],"(R.":[33],"G.":[34],"Wells":[35],"M.":[37],"A.":[38],"Hediger.":[39],"Proc.":[40],"Natl.":[41],"Acad.":[42],"Sci.":[43],"USA":[44],"89:":[45],"5596-5600,":[46],"1992).":[47],"At":[48],"present":[49],"it":[50],"is":[51,150,163,174],"not":[52],"known":[53],"whether":[54],"the":[55,72,83,89,122,140,154,167,181,192,205],"D2-encoded":[56],"protein":[57],"functions":[58],"as":[59,63],"a":[60,64,101],"transporter":[61,65,143],"or":[62],"activator.":[66],"To":[67],"gain":[68],"more":[69],"insight":[70],"role":[73],"D2":[75,117,148,188,209],"renal":[77],"acid":[79],"transport,":[80],"we":[81],"studied":[82],"site":[84,193],"its":[86],"kidney.":[90,182],"This":[91],"determined":[93],"Northern":[95],"blot":[96],"analysis":[97],"using":[100],"combination":[102],"situ":[105],"hybridization":[106,165],"immunocytochemistry":[108],"with":[109,191,204],"antibodies":[110],"recognize":[112],"specific":[113,185],"proximal":[114,157],"tubule":[115,158],"segments.":[116,171],"antisense":[118],"RNA":[119],"hybridized":[120],"to":[121,166],"same":[123],"tubular":[124],"segments":[125],"were":[127],"strongly":[128,151],"positive":[129],"for":[130,134],"anti-ecto-adenosinetriphosphatase":[131],"but":[132],"negative":[133],"carbonic":[135],"anhydrase":[136],"type":[137],"IV":[138],"facilitated":[141],"glucose":[142],"GLUT2.":[144],"We":[145],"conclude":[146],"mRNA":[149,189],"expressed":[152],"kidney":[156],"S3":[159,184],"segment,":[160],"although":[161],"there":[162],"weak":[164],"S1":[168],"S2":[170],"The":[172,183],"signal":[173],"absent":[175],"all":[177],"other":[178,200],"parts":[179],"coincides":[190],"high-affinity":[195],"transport":[196],"acids,":[202],"consistent":[203],"proposed":[206],"involvement":[207],"these":[211],"processes.":[212]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}