{"id":"https://openalex.org/W2406091709","doi":null,"title":"Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding.","display_name":"Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding.","publication_year":1994,"publication_date":"1994-05-01","ids":{"openalex":"https://openalex.org/W2406091709","mag":"2406091709","pmid":"https://pubmed.ncbi.nlm.nih.gov/8195679"},"language":"en","primary_location":{"id":"pmid:8195679","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8195679","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of laboratory and clinical medicine","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109897083","display_name":"Jonathan F. Tait","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J F Tait","raw_affiliation_strings":["Department of Laboratory Medicine, University of Washington, Seattle 98195"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, University of Washington, Seattle 98195","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":null,"display_name":"D Gibson","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D Gibson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.0933,"has_fulltext":false,"cited_by_count":150,"citation_normalized_percentile":{"value":0.94600409,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"123","issue":"5","first_page":"741","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12286","display_name":"Erythrocyte Function and Pathophysiology","score":0.5088000297546387,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12286","display_name":"Erythrocyte Function and Pathophysiology","score":0.5088000297546387,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12030","display_name":"S100 Proteins and Annexins","score":0.33469998836517334,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.05260000005364418,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/membrane-phospholipid-asymmetry","display_name":"membrane phospholipid asymmetry","score":0.9670000076293945},{"id":"https://openalex.org/keywords/annexin-v","display_name":"annexin V","score":0.7390000224113464},{"id":"https://openalex.org/keywords/sickle-cell-disease","display_name":"sickle cell disease","score":0.8370000123977661},{"id":"https://openalex.org/keywords/erythrocytes","display_name":"erythrocytes","score":0.8849999904632568},{"id":"https://openalex.org/keywords/phosphatidylserine-exposure","display_name":"phosphatidylserine exposure","score":0.9729999899864197},{"id":"https://openalex.org/keywords/calcium-dependent-binding","display_name":"calcium-dependent binding","score":0.7730000019073486},{"id":"https://openalex.org/keywords/a23187","display_name":"A23187","score":0.8669999837875366},{"id":"https://openalex.org/keywords/red-blood-cell-storage","display_name":"red blood cell storage","score":0.8939999938011169},{"id":"https://openalex.org/keywords/vaso-occlusive-crisis","display_name":"vaso-occlusive crisis","score":0.9729999899864197},{"id":"https://openalex.org/keywords/whole-blood-assay","display_name":"whole blood assay","score":0.5419999957084656}],"concepts":[{"id":"https://openalex.org/C88634738","wikidata":"https://www.wikidata.org/wiki/Q24769543","display_name":"Annexin","level":3,"score":0.8127857446670532},{"id":"https://openalex.org/C2779637612","wikidata":"https://www.wikidata.org/wiki/Q2354337","display_name":"Phosphatidylserine","level":4,"score":0.7609527707099915},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.6867326498031616},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.6096574068069458},{"id":"https://openalex.org/C2780670749","wikidata":"https://www.wikidata.org/wiki/Q14908239","display_name":"Annexin A5","level":4,"score":0.5962960720062256},{"id":"https://openalex.org/C519063684","wikidata":"https://www.wikidata.org/wiki/Q706","display_name":"Calcium","level":2,"score":0.5816013216972351},{"id":"https://openalex.org/C30345302","wikidata":"https://www.wikidata.org/wiki/Q14908233","display_name":"Annexin A2","level":4,"score":0.5425111055374146},{"id":"https://openalex.org/C2776557347","wikidata":"https://www.wikidata.org/wiki/Q37187","display_name":"Red blood cell","level":2,"score":0.5133673548698425},{"id":"https://openalex.org/C25642318","wikidata":"https://www.wikidata.org/wiki/Q1992532","display_name":"Incubation","level":2,"score":0.5121047496795654},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4876083433628082},{"id":"https://openalex.org/C206396517","wikidata":"https://www.wikidata.org/wiki/Q422025","display_name":"Haptoglobin","level":2,"score":0.48663529753685},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4830970764160156},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.45841073989868164},{"id":"https://openalex.org/C74998103","wikidata":"https://www.wikidata.org/wiki/Q898254","display_name":"Dissociation constant","level":3,"score":0.4483107030391693},{"id":"https://openalex.org/C2777208419","wikidata":"https://www.wikidata.org/wiki/Q4347672","display_name":"Red Cell","level":2,"score":0.4459300637245178},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.44571825861930847},{"id":"https://openalex.org/C2778106830","wikidata":"https://www.wikidata.org/wiki/Q29548","display_name":"Cell membrane","level":3,"score":0.421374648809433},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4171314239501953},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4064098298549652},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3485184609889984},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.30421018600463867},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.2732187807559967},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.14309540390968323},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.1108635663986206},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000001","descriptor_name":"Calcimycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004912","descriptor_name":"Erythrocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008563","descriptor_name":"Membrane Lipids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010718","descriptor_name":"Phosphatidylserines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010743","descriptor_name":"Phospholipids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012016","descriptor_name":"Reference Values","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012805","descriptor_name":"Sickle Cell Trait","qualifier_ui":"Q000097","qualifier_name":"blood","is_major_topic":true},{"descriptor_ui":"D017304","descriptor_name":"Annexin A5","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:8195679","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8195679","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of laboratory and clinical medicine","raw_type":"Journal Article"},{"id":"mag:2406091709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8195679","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8444437558","display_name":"SCOR IN ARTERIOSCLEROSIS","funder_award_id":"P50HL047151","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G944517122","display_name":"CHARACTERIZATION OF PLACENTAL ANTICOAGULANT PROTEIN","funder_award_id":"R29HL040801","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W105021832","https://openalex.org/W7097850042","https://openalex.org/W13114315","https://openalex.org/W58418599","https://openalex.org/W2016582110","https://openalex.org/W2006203283","https://openalex.org/W2168637017","https://openalex.org/W2053239368","https://openalex.org/W2014341094","https://openalex.org/W1997934608"],"abstract_inverted_index":{"We":[0,113],"investigated":[1],"the":[2,78],"use":[3],"of":[4,71,80,102,111,121,128,134,165,181,191,209,237],"annexin":[5,33,68,95,122,192,231],"V":[6,34,96,123,193,232],"(placental":[7],"anticoagulant":[8],"protein":[9,13],"I),":[10],"a":[11,21,39,89,100,116,132,162,203,220,227,235],"calcium-dependent":[12,42],"that":[14],"binds":[15],"tightly":[16],"to":[17,23,36,174,195],"phosphatidylserine-containing":[18],"membranes,":[19],"as":[20],"means":[22],"measure":[24],"membrane":[25],"phospholipid":[26],"asymmetry":[27],"in":[28,38,77,94,206,219,230],"human":[29],"erythrocytes.":[30],"Iodine":[31],"125-labeled":[32],"bound":[35],"erythrocytes":[37,60,72],"specific,":[40],"reversible,":[41],"reaction":[43],"(dissociation":[44],"constant":[45],"=":[46],"25":[47],"+/-":[48],"4":[49,184],"nmol/L":[50],"at":[51,84,183],"37":[52,85],"degrees":[53,86,185],"C":[54,87,186],"and":[55],"2.5":[56,81],"mmol/L":[57,82],"calcium).":[58],"Lysed":[59],"contained":[61],"1.2":[62],"x":[63],"10(6)":[64],"binding":[65,97,124,194,207,233],"sites":[66,105,125,137,167,176,211],"for":[67,187,214],"V.":[69],"Treatment":[70],"with":[73,199,223],"1":[74],"mumol/L":[75],"A23187":[76],"presence":[79],"calcium":[83],"caused":[88],"gradual":[90,118],"but":[91],"marked":[92,204],"increase":[93,205],"sites,":[98],"reaching":[99,131],"level":[101,133],"approximately":[103,135],"300,000":[104],"per":[106,138,168,177,212],"cell":[107,139,169,178,213],"after":[108,140,179],"8":[109],"hours":[110],"incubation.":[112],"also":[114,145],"noted":[115],"very":[117],"spontaneous":[119],"exposure":[120],"during":[126],"storage":[127,180],"purified":[129],"erythrocytes,":[130],"20,000":[136],"30":[141],"days.":[142],"Measurements":[143],"could":[144],"be":[146],"made":[147],"directly":[148],"on":[149],"diluted":[150],"whole":[151],"blood":[152],"specimens.":[153],"In":[154],"samples":[155,196],"freshly":[156],"drawn":[157],"from":[158,197],"35":[159],"normal":[160],"donors,":[161],"mean":[163],"number":[164],"276":[166],"were":[170],"present;":[171],"this":[172],"increased":[173],"858":[175],"specimens":[182],"24":[188],"hours.":[189],"Measurement":[190],"patients":[198],"sickle-cell":[200,224],"anemia":[201],"revealed":[202],"(mean":[208],"12,430":[210],"all":[215],"samples);":[216],"serial":[217],"measurements":[218],"patient":[221],"hospitalized":[222],"crisis":[225],"showed":[226],"progressive":[228],"decline":[229],"over":[234],"period":[236],"6":[238],"days.(ABSTRACT":[239],"TRUNCATED":[240],"AT":[241],"250":[242],"WORDS)":[243]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":4},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}