{"id":"https://openalex.org/W2406071850","doi":null,"title":"Temporal and spatial expression of lipospermine-compacted genes transferred into chick embryos in vivo.","display_name":"Temporal and spatial expression of lipospermine-compacted genes transferred into chick embryos in vivo.","publication_year":1994,"publication_date":"1994-03-01","ids":{"openalex":"https://openalex.org/W2406071850","mag":"2406071850","pmid":"https://pubmed.ncbi.nlm.nih.gov/8185924"},"language":"en","primary_location":{"id":"pmid:8185924","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8185924","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Comparative Study"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003494511","display_name":"BARBARA A. DEMENEIX","orcid":"https://orcid.org/0000-0003-4544-971X","observed_orcids":["https://orcid.org/0000-0003-4544-971X"]},"institutions":[{"id":"https://openalex.org/I4210123595","display_name":"Mus\u00e9um national d'Histoire naturelle","ror":"https://ror.org/03wkt5x30","country_code":"FR","type":"archive","lineage":["https://openalex.org/I4210123595"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"B A Demeneix","raw_affiliation_strings":["Mus\u00e9um Nationale d'Histoire Naturelle, Paris, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mus\u00e9um Nationale d'Histoire Naturelle, Paris, France","institution_ids":["https://openalex.org/I4210123595"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030007542","display_name":"H Abdel-Taweb","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Abdel-Taweb","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112005715","display_name":"C Benoist","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C Benoist","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066918932","display_name":"Isabelle Seugnet","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I Seugnet","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5107196450","display_name":"J.\u2010P. BEHR","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J P Behr","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.6447,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.84588603,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"16","issue":"3","first_page":"496","last_page":"501"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11455","display_name":"Microbial Inactivation Methods","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cationic-lipid-transfection","display_name":"cationic lipid transfection","score":0.8420000076293945},{"id":"https://openalex.org/keywords/gene-delivery","display_name":"gene delivery","score":0.41200000047683716},{"id":"https://openalex.org/keywords/chick-embryo","display_name":"chick embryo","score":0.8999999761581421},{"id":"https://openalex.org/keywords/in-vivo-gene-transfer","display_name":"in vivo gene delivery","score":0.7680000066757202},{"id":"https://openalex.org/keywords/luciferase-reporter","display_name":"luciferase reporter","score":0.8090000152587891},{"id":"https://openalex.org/keywords/lacz-reporter","display_name":"lacZ reporter","score":0.9049999713897705},{"id":"https://openalex.org/keywords/central-nervous-system-expression","display_name":"central nervous system expression","score":0.7710000276565552},{"id":"https://openalex.org/keywords/spatiotemporal-gene-expression","display_name":"spatiotemporal gene expression","score":0.6359999775886536},{"id":"https://openalex.org/keywords/embryonic-development","display_name":"embryonic development","score":0.6380000114440918}],"concepts":[{"id":"https://openalex.org/C2777441520","wikidata":"https://www.wikidata.org/wiki/Q1444330","display_name":"Rous sarcoma virus","level":3,"score":0.7957606911659241},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7617393136024475},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7344767451286316},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.7149357795715332},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.6608643531799316},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.6588422656059265},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6386407613754272},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.5885074138641357},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5555539131164551},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5447210073471069},{"id":"https://openalex.org/C182420341","wikidata":"https://www.wikidata.org/wiki/Q10860423","display_name":"Exogenous DNA","level":3,"score":0.5245937705039978},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5124809741973877},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.432364821434021},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.40464046597480774},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2855834364891052},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.1508331000804901}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001358","descriptor_name":"Avian Sarcoma Viruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":false},{"descriptor_ui":"D005998","descriptor_name":"Glycine","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":false},{"descriptor_ui":"D005998","descriptor_name":"Glycine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D006321","descriptor_name":"Heart","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008845","descriptor_name":"Microinjections","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009206","descriptor_name":"Myocardium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011401","descriptor_name":"Promoter Regions, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013096","descriptor_name":"Spermine","qualifier_ui":"Q000008","qualifier_name":"administration & dosage","is_major_topic":false},{"descriptor_ui":"D013096","descriptor_name":"Spermine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:8185924","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8185924","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Comparative Study"},{"id":"mag:2406071850","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8185924","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2028888853","https://openalex.org/W2037366276","https://openalex.org/W2438287524","https://openalex.org/W2069180128","https://openalex.org/W2060188519","https://openalex.org/W2122618416","https://openalex.org/W2166876266","https://openalex.org/W2036786328","https://openalex.org/W2317254921","https://openalex.org/W3149771895"],"abstract_inverted_index":{"We":[0],"have":[1],"optimized":[2],"a":[3,28,112],"lipospermine-based":[4],"transfection":[5,122],"method":[6,17],"for":[7],"introducing":[8],"genes":[9,40,163,189,201],"into":[10,41,154,190],"intact":[11],"vertebrate":[12],"embryos":[13,43,191],"in":[14,27,168,202,206,208],"vivo.":[15],"The":[16,158],"employs":[18],"small":[19],"amounts":[20],"of":[21,48,53,69,108,134,195],"the":[22,45,58,66,92,105,109,121,128,131,137,145,150,155,170,179],"cationic":[23],"lipid":[24],"Transfectam":[25],"(DOGS),":[26],"concentrated":[29],"(40":[30],"mM)":[31],"ethanolic":[32],"solution,":[33],"to":[34,37,64,144,186,199],"compact":[35],"and":[36,81,96,175,197,205],"transfer":[38,187],"exogenous":[39,110],"chick":[42],"during":[44,192],"early":[46,76],"stages":[47],"development":[49,196],"(<":[50],"36":[51],"h":[52,79],"incubation).":[54],"Plasmid":[55],"vectors":[56],"containing":[57],"reporter":[59,162],"gene":[60],"luciferase":[61],"were":[62],"used":[63,185],"follow":[65,104],"time":[67],"course":[68],"expression.":[70],"Luciferase":[71],"activity":[72,88],"was":[73,82,97,118,124,136],"detected":[74],"as":[75,77],"12":[78],"post-transfection":[80],"highest":[83],"at":[84],"this":[85],"time.":[86],"Enzyme":[87],"then":[89],"decreased":[90],"over":[91],"next":[93],"two":[94],"days":[95],"usually":[98],"undetectable":[99],"by":[100,148],"72-h":[101],"post-transfection.":[102],"To":[103],"spatial":[106],"expression":[107,135],"genes,":[111],"Rous":[113],"sarcoma":[114],"virus":[115],"(RSV)-beta-galactosidase":[116],"vector":[117],"used.":[119],"When":[120],"complex":[123,153],"applied":[125],"externally":[126],"around":[127],"developing":[129,156,171],"embryo,":[130],"main":[132],"site":[133],"cardiac":[138],"tissue.":[139],"Expression":[140],"could":[141],"be":[142,165,184],"targeted":[143],"nervous":[146,173],"system":[147,174],"micro-injecting":[149],"DNA/DOGS":[151],"(DNA/dioctadecylamidoglycylspermine)":[152],"brain.":[157],"results":[159],"show":[160],"that":[161,181],"can":[164,183],"efficiently":[166],"expressed":[167],"both":[169],"central":[172],"heart.":[176],"This":[177],"raises":[178],"possibility":[180],"lipospermines":[182],"functional":[188],"defined":[193],"periods":[194],"also":[198],"deliver":[200],"other":[203,207],"species":[204],"vivo":[209],"contexts.":[210]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}