{"id":"https://openalex.org/W2399171998","doi":null,"title":"Fiber type, intramyocellular lipid and mitochondrial mass in human primary myotubes retain the metabolic characteristics of the donor","display_name":"Fiber type, intramyocellular lipid and mitochondrial mass in human primary myotubes retain the metabolic characteristics of the donor","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2399171998","mag":"2399171998"},"language":"en","primary_location":{"id":"pmh:oai:www.duo.uio.no:10852/11947","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10852/11947","pdf_url":null,"source":{"id":"https://openalex.org/S7407064446","display_name":"NORA - Norwegian Open Research Archives","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10852/11947","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027777801","display_name":"Cassandra Kim Myrland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Cassandra Kim Myrland","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5027777801"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.7310000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.7310000061988831,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11457","display_name":"Adipose Tissue and Metabolism","score":0.13330000638961792,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11399","display_name":"Muscle metabolism and nutrition","score":0.03610000014305115,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/skeletal-muscle-fiber-type-composition","display_name":"skeletal muscle fiber type composition","score":0.8240000009536743},{"id":"https://openalex.org/keywords/type-i-muscle-fibres","display_name":"type I muscle fibers","score":0.7490000128746033},{"id":"https://openalex.org/keywords/intramyocellular-lipid","display_name":"intramyocellular lipid","score":0.9890000224113464},{"id":"https://openalex.org/keywords/mitochondrial-mass","display_name":"mitochondrial mass","score":0.9919999837875366},{"id":"https://openalex.org/keywords/type-2-diabetes","display_name":"type 2 diabetes","score":0.8999999761581421},{"id":"https://openalex.org/keywords/athletes","display_name":"athletes","score":0.984000027179718},{"id":"https://openalex.org/keywords/vastus-lateralis","display_name":"vastus lateralis","score":0.7590000033378601},{"id":"https://openalex.org/keywords/insulin-sensitivity","display_name":"insulin sensitivity","score":0.722000002861023},{"id":"https://openalex.org/keywords/physical-fitness","display_name":"physical fitness","score":0.7789999842643738},{"id":"https://openalex.org/keywords/vo2max","display_name":"VO2max","score":0.8199999928474426},{"id":"https://openalex.org/keywords/physical-activity-level","display_name":"physical activity level","score":0.8930000066757202},{"id":"https://openalex.org/keywords/muscle-metabolism","display_name":"muscle metabolism","score":0.515999972820282},{"id":"https://openalex.org/keywords/myogenesis","display_name":"myogenesis","score":0.5950000286102295},{"id":"https://openalex.org/keywords/hyperinsulinemic-euglycemic-clamp","display_name":"hyperinsulinemic-euglycemic clamp","score":0.8209999799728394},{"id":"https://openalex.org/keywords/mitochondrial-biogenesis","display_name":"mitochondrial biogenesis","score":0.35499998927116394},{"id":"https://openalex.org/keywords/lipid-metabolism","display_name":"lipid metabolism","score":0.367000013589859},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.3479999899864197},{"id":"https://openalex.org/keywords/mitochondrial-fission-and-fusion","display_name":"mitochondrial fission and fusion","score":0.36899998784065247}],"concepts":[{"id":"https://openalex.org/C3763915","wikidata":"https://www.wikidata.org/wiki/Q3331437","display_name":"Myogenesis","level":3,"score":0.7477710247039795},{"id":"https://openalex.org/C519885992","wikidata":"https://www.wikidata.org/wiki/Q161","display_name":"Fiber","level":2,"score":0.4929361343383789},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4331055283546448},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3443831503391266},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3433363735675812},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.16941487789154053},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:www.duo.uio.no:10852/11947","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10852/11947","pdf_url":null,"source":{"id":"https://openalex.org/S7407064446","display_name":"NORA - Norwegian Open Research Archives","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},{"id":"mag:2399171998","doi":null,"is_oa":false,"landing_page_url":"https://www.duo.uio.no/bitstream/10852/11947/4/Cassandra_Kim_Myrland_Masteroppgave.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:www.duo.uio.no:10852/11947","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10852/11947","pdf_url":null,"source":{"id":"https://openalex.org/S7407064446","display_name":"NORA - Norwegian Open Research Archives","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/masterThesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":74,"referenced_works":["https://openalex.org/W246850424","https://openalex.org/W1857724021","https://openalex.org/W1894420075","https://openalex.org/W1966509583","https://openalex.org/W1969927905","https://openalex.org/W1971141707","https://openalex.org/W1979255869","https://openalex.org/W1981124323","https://openalex.org/W1988447258","https://openalex.org/W1993877486","https://openalex.org/W1995836919","https://openalex.org/W2005070668","https://openalex.org/W2008430800","https://openalex.org/W2018118901","https://openalex.org/W2020090231","https://openalex.org/W2020267609","https://openalex.org/W2020575559","https://openalex.org/W2024907664","https://openalex.org/W2027670788","https://openalex.org/W2028211887","https://openalex.org/W2029197669","https://openalex.org/W2032861840","https://openalex.org/W2036227669","https://openalex.org/W2038143626","https://openalex.org/W2038961236","https://openalex.org/W2056114428","https://openalex.org/W2058523643","https://openalex.org/W2060431825","https://openalex.org/W2064428048","https://openalex.org/W2068205637","https://openalex.org/W2070004295","https://openalex.org/W2073935988","https://openalex.org/W2075703747","https://openalex.org/W2086849804","https://openalex.org/W2088490069","https://openalex.org/W2091224218","https://openalex.org/W2091798744","https://openalex.org/W2093366966","https://openalex.org/W2097408175","https://openalex.org/W2098384661","https://openalex.org/W2099385136","https://openalex.org/W2100580265","https://openalex.org/W2100912424","https://openalex.org/W2110542127","https://openalex.org/W2112522013","https://openalex.org/W2112991269","https://openalex.org/W2113087113","https://openalex.org/W2114041992","https://openalex.org/W2114725982","https://openalex.org/W2122186843","https://openalex.org/W2125552035","https://openalex.org/W2127262263","https://openalex.org/W2129876483","https://openalex.org/W2131029957","https://openalex.org/W2131316894","https://openalex.org/W2133810978","https://openalex.org/W2134293572","https://openalex.org/W2135514341","https://openalex.org/W2135667395","https://openalex.org/W2142483199","https://openalex.org/W2145891389","https://openalex.org/W2148077640","https://openalex.org/W2148713009","https://openalex.org/W2148824946","https://openalex.org/W2152958952","https://openalex.org/W2154768987","https://openalex.org/W2155605721","https://openalex.org/W2157037423","https://openalex.org/W2158254429","https://openalex.org/W2162473621","https://openalex.org/W2168727368","https://openalex.org/W2196373679","https://openalex.org/W2329425391","https://openalex.org/W2893569439"],"related_works":["https://openalex.org/W1595520227","https://openalex.org/W2139664139","https://openalex.org/W1944608843","https://openalex.org/W2212452974","https://openalex.org/W2168498537","https://openalex.org/W2955721958","https://openalex.org/W7150982218","https://openalex.org/W2948576253","https://openalex.org/W2068342735","https://openalex.org/W1997246808"],"abstract_inverted_index":{"Fiber":[0],"type":[1,43,306],"composition":[2],"is":[3],"a":[4,150,283],"significant":[5,151,260],"and":[6,28,35,51,62,90,98,119,125,136,173,189,205,252,269,292,334,337],"critical":[7],"characteristic":[8],"of":[9,14,79,82,154,308,339],"skeletal":[10,309],"muscle.":[11],"The":[12],"purpose":[13],"this":[15],"study":[16],"was":[17,141,218,272,286,328],"to":[18,36,184,279],"investigate":[19],"the":[20,71,77,340],"differences":[21],"in":[22,25,31,54,57,66,195,203,216,226,231,274,288,311,315,325],"metabolic":[23,38],"characteristics":[24,39,307],"athletes,":[26,94],"lean":[27,97,188],"T2DM":[29,102,190],"determined":[30,76],"human":[32],"primary":[33,67,302],"myotubes,":[34,317],"compare":[37],"such":[40],"as":[41,211],"fiber":[42,60,85,124,155,192,199,264,289,305],"composition,":[44],"lipid":[45,63,88,126,164,223,229,267],"content,":[46,87,266],"mitochondrial":[47,91,174,208,270,293],"capacity,":[48],"physical":[49,245,253,335],"fitness":[50,336],"activity":[52,254,338],"measured":[53,65,142,194,201,210,215,225,230,238,247,314,324],"previous":[55],"studies":[56],"vivo":[58],"versus":[59],"type-I":[61,86,156,193,200,265,290],"content":[64,89,127,165,214,268,323],"myotubes":[68,83,185,196,217,232,275,303],"cultured":[69,118,186,316],"from":[70,109,113,147,187],"same":[72],"donors.":[73],"Moreover,":[74],"we":[75],"effect":[78,262],"progressive":[80],"passaging":[81],"on":[84,263],"mass.":[92],"\\n\\nSix":[93],"6":[95,99],"sedentary":[96],"subjects":[100],"with":[101,198,207,221,235,296,321,331],"were":[103,107,116,128],"studied.":[104],"Muscle":[105],"samples":[106],"obtained":[108],"Vastus":[110],"Lateralis":[111],"(VL)":[112],"which":[114],"myoblasts":[115],"sorted,":[117],"differentiated":[120],"into":[121],"myotubes.":[122],"Type-I":[123],"quantified":[129],"by":[130,143,239,248],"immunohistochemistry":[131],"technique":[132],"using":[133],"MHC-I":[134],"antibody":[135],"bodipy":[137],"respectively.":[138],"Mitochondrial":[139],"mass":[140,175,271,294],"Mitotracker":[144],"Green.":[145],"\\n\\nMyotubes":[146],"athletes":[148],"had":[149],"higher":[152],"amount":[153],"(9.9\u00b11.8":[157],"vs.":[158,160,167,169,177,179],"1.6\u00b11.0":[159],"2.0\u00b10.8":[161],"AU;":[162,171,181],"p<0.001),":[163],"(0.047\u00b10.003":[166],"0.032\u00b10.001":[168],"0.033\u00b10.001":[170],"p<0.001)":[172],"(105.2\u00b13.8":[176],"80.0\u00b13.0":[178],"90.0\u00b13.9":[180],"p<0.01)":[182],"compared":[183],"subjects;":[191],"correlated":[197,220,234],"directly":[202],"tissue":[204],"also":[206],"capacity":[209],"ATPmax.":[212],"Lipid":[213,313],"not":[219,319],"intramyocellular":[222],"(IMCL)":[224],"tissue.":[227],"However,":[228,282],"strongly":[233,329],"insulin":[236,332],"sensitivity":[237,333],"hyperinsulinemic":[240],"euglycemic":[241],"clamp":[242],"(r2=0.29;":[243],"p<0.05),":[244],"fitness,":[246],"VO2max":[249],"(r2=0.54;":[250],"p<0.05)":[251],"level":[255],"(PAL)":[256],"(r2=0.85;":[257],"p<0.01).":[258],"No":[259],"passage":[261,277,280],"observed":[273,287],"through":[276],"4":[278],"7.":[281],"decreasing":[284],"trend":[285],"(P=0.14)":[291],"(P=0.05)":[295],"multiple":[297],"passaging.":[298],"\\n":[299],"\\nIn":[300],"conclusion,":[301],"retain":[304],"muscle":[310],"vivo.":[312],"although":[318],"associated":[320,330],"IMCL":[322],"tissue,":[326],"it":[327],"donor.":[341]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}