{"id":"https://openalex.org/W2398375261","doi":null,"title":"Recapitulation of Werner syndrome sensitivity to camptothecin by limited knockdown of the WRN","display_name":"Recapitulation of Werner syndrome sensitivity to camptothecin by limited knockdown of the WRN","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2398375261","mag":"2398375261"},"language":"en","primary_location":{"id":"mag:2398375261","doi":null,"is_oa":false,"landing_page_url":"https://link.springer.com/article/10.1007/s10522-011-9341-8","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013330651","display_name":"Andreas Bachler","orcid":"https://orcid.org/0009-0000-0765-3109","observed_orcids":["https://orcid.org/0009-0000-0765-3109"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Andreas Bachler","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0000-0765-3109","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5004161736","display_name":"Stephanie W. Cox","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Stephanie Cox","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11845","display_name":"Cancer therapeutics and mechanisms","score":0.8389000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11845","display_name":"Cancer therapeutics and mechanisms","score":0.8389000296592712,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.07670000195503235,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.05510000139474869,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/werner-syndrome","display_name":"Werner syndrome","score":0.9950000047683716},{"id":"https://openalex.org/keywords/wrn-helicase","display_name":"WRN helicase","score":0.9490000009536743},{"id":"https://openalex.org/keywords/camptothecin-sensitivity","display_name":"camptothecin sensitivity","score":0.9120000004768372},{"id":"https://openalex.org/keywords/topoisomerase-inhibitors","display_name":"topoisomerase inhibitors","score":0.8669999837875366},{"id":"https://openalex.org/keywords/recq-helicase","display_name":"RecQ helicase","score":0.796999990940094},{"id":"https://openalex.org/keywords/rnai-knockdown","display_name":"RNAi knockdown","score":0.7210000157356262},{"id":"https://openalex.org/keywords/hammerhead-ribozyme","display_name":"hammerhead ribozyme","score":0.9959999918937683},{"id":"https://openalex.org/keywords/replicative-senescence","display_name":"replicative senescence","score":0.9100000262260437},{"id":"https://openalex.org/keywords/chromosomal-instability","display_name":"chromosomal instability","score":0.9330000281333923},{"id":"https://openalex.org/keywords/cell-cycle-arrest","display_name":"cell cycle arrest","score":0.6710000038146973},{"id":"https://openalex.org/keywords/progeroid-phenotype","display_name":"progeroid phenotype","score":0.45399999618530273}],"concepts":[{"id":"https://openalex.org/C2778090637","wikidata":"https://www.wikidata.org/wiki/Q1154619","display_name":"Werner syndrome","level":5,"score":0.8215969204902649},{"id":"https://openalex.org/C2779682216","wikidata":"https://www.wikidata.org/wiki/Q419964","display_name":"Camptothecin","level":2,"score":0.7340297698974609},{"id":"https://openalex.org/C178169997","wikidata":"https://www.wikidata.org/wiki/Q2892636","display_name":"Genome instability","level":4,"score":0.6761667132377625},{"id":"https://openalex.org/C161223559","wikidata":"https://www.wikidata.org/wiki/Q138864","display_name":"Helicase","level":4,"score":0.6570887565612793},{"id":"https://openalex.org/C2909947164","wikidata":"https://www.wikidata.org/wiki/Q6139748","display_name":"Premature aging","level":2,"score":0.5885968208312988},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5836664438247681},{"id":"https://openalex.org/C2780751114","wikidata":"https://www.wikidata.org/wiki/Q7302254","display_name":"RecQ helicase","level":5,"score":0.536902904510498},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.5352891683578491},{"id":"https://openalex.org/C2778480876","wikidata":"https://www.wikidata.org/wiki/Q21112674","display_name":"PARP1","level":5,"score":0.508614182472229},{"id":"https://openalex.org/C134935766","wikidata":"https://www.wikidata.org/wiki/Q210538","display_name":"DNA repair","level":3,"score":0.5008351802825928},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.479636013507843},{"id":"https://openalex.org/C2777501222","wikidata":"https://www.wikidata.org/wiki/Q411664","display_name":"Exonuclease","level":4,"score":0.46863293647766113},{"id":"https://openalex.org/C140752955","wikidata":"https://www.wikidata.org/wiki/Q1087752","display_name":"Chromosome instability","level":4,"score":0.4533585011959076},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.44471901655197144},{"id":"https://openalex.org/C147897179","wikidata":"https://www.wikidata.org/wiki/Q407828","display_name":"Topoisomerase","level":3,"score":0.4389362037181854},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.40595072507858276},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3823740780353546},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3432806730270386},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2723901569843292},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.1697092354297638},{"id":"https://openalex.org/C182979987","wikidata":"https://www.wikidata.org/wiki/Q3907241","display_name":"Poly ADP ribose polymerase","level":4,"score":0.12435173988342285},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11706051230430603},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.08232492208480835},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08191913366317749},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.0681656002998352},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.0},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2398375261","doi":null,"is_oa":false,"landing_page_url":"https://link.springer.com/article/10.1007/s10522-011-9341-8","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W1538603370","https://openalex.org/W1965282338","https://openalex.org/W1966347623","https://openalex.org/W1968460725","https://openalex.org/W1974481403","https://openalex.org/W1990557525","https://openalex.org/W2006322108","https://openalex.org/W2008496459","https://openalex.org/W2008537032","https://openalex.org/W2010714957","https://openalex.org/W2023243457","https://openalex.org/W2026766143","https://openalex.org/W2029204193","https://openalex.org/W2037563286","https://openalex.org/W2060717672","https://openalex.org/W2061553377","https://openalex.org/W2064310741","https://openalex.org/W2076559543","https://openalex.org/W2082605431","https://openalex.org/W2082947047","https://openalex.org/W2084712319","https://openalex.org/W2085639710","https://openalex.org/W2086589342","https://openalex.org/W2097882687","https://openalex.org/W2108715799","https://openalex.org/W2110145918","https://openalex.org/W2140716778","https://openalex.org/W2142629409","https://openalex.org/W2153043528","https://openalex.org/W2153747430","https://openalex.org/W2155835896","https://openalex.org/W2163819508","https://openalex.org/W2166344257","https://openalex.org/W2166359461","https://openalex.org/W2171109371","https://openalex.org/W2470721145"],"related_works":["https://openalex.org/W2099708345","https://openalex.org/W2293960364","https://openalex.org/W2026766143","https://openalex.org/W2085639710","https://openalex.org/W2029204193","https://openalex.org/W2028408120","https://openalex.org/W3042145981","https://openalex.org/W1970285696","https://openalex.org/W2165666807","https://openalex.org/W2025255779"],"abstract_inverted_index":{"WRN":[0,26,52,86,100,153,159],"is":[1],"a":[2,89],"RecQ":[3],"helicase":[4],"with":[5],"an":[6],"associ-":[7],"ated":[8],"exonuclease":[9],"activity":[10],"important":[11],"in":[12,25,35,55,135,151,184],"DNA":[13,17,65],"metab-":[14],"olism,":[15],"including":[16],"replication,":[18],"repair":[19],"and":[20,61,124,132],"recombination.":[21],"In":[22],"humans,":[23],"deficiencies":[24],"function":[27],"cause":[28],"the":[29,49,70,78,164],"segmental":[30],"progeroid":[31],"Werner":[32],"syndrome":[33],"(WS),":[34],"which":[36],"patients":[37],"show":[38,97],"premature":[39],"onset":[40],"of":[41,44,80,91,158,177],"many":[42],"hallmarks":[43],"normal":[45],"human":[46,112],"ageing.":[47],"At":[48],"cellular":[50,165],"level,":[51],"loss":[53],"results":[54],"rapid":[56],"replicative":[57],"senescence,":[58],"chromosomal":[59],"instability":[60],"sensitivity":[62],"to":[63,94,116,167,188],"various":[64],"damaging":[66],"agents":[67,179],"includ-":[68],"ing":[69],"topoisomerase":[71,191],"inhibitor,":[72],"camptothecin":[73],"(CPT).":[74],"Here,":[75],"we":[76,126],"investigate":[77],"potential":[79],"using":[81],"either":[82,105],"transient":[83],"or":[84,107],"stable":[85],"knockdown":[87],"as":[88,114,118],"means":[90],"sensitising":[92,185],"cells":[93,137,187],"CPT.":[95],"We":[96],"that":[98,156,180],"targeting":[99],"mRNA":[101],"for":[102,163,175],"degradation":[103],"by":[104],"RNAi":[106],"hammerhead":[108],"ribozyme":[109],"catalysis":[110],"renders":[111],"fibroblasts":[113,119],"sensitive":[115],"CPT":[117,139],"derived":[120],"from":[121],"WS":[122],"patients,":[123],"furthermore,":[125],"find":[127],"altered":[128],"cell":[129],"cycle":[130],"transit":[131],"nucleolar":[133],"destabilisation":[134],"these":[136],"following":[138],"treatment.":[140],"Such":[141],"WS-like":[142],"pheno-":[143],"types":[144],"are":[145,161],"observed":[146],"despite":[147],"very":[148],"limited":[149],"decreases":[150],"total":[152],"protein,":[154],"suggesting":[155],"levels":[157],"protein":[160],"rate-limiting":[162],"response":[166],"camptothecin.":[168],"These":[169],"findings":[170],"have":[171],"major":[172],"implica-":[173],"tions":[174],"development":[176],"anti-WRN":[178],"may":[181],"be":[182],"useful":[183],"tumour":[186],"clinically":[189],"relevant":[190],"inhibitors.":[192]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}