{"id":"https://openalex.org/W2398024636","doi":"https://doi.org/10.1385/1-59259-973-7:307","title":"32P-Postlabeling DNA Damage Assays","display_name":"32P-Postlabeling DNA Damage Assays","publication_year":2008,"publication_date":"2008-09-19","ids":{"openalex":"https://openalex.org/W2398024636","doi":"https://doi.org/10.1385/1-59259-973-7:307","mag":"2398024636","pmid":"https://pubmed.ncbi.nlm.nih.gov/16673890"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-973-7:307","doi":"https://doi.org/10.1385/1-59259-973-7:307","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-973-7:307","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4239115684","display_name":"DNA Repair Protocols"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063749811","display_name":"Shinya Shibutani","orcid":"https://orcid.org/0000-0003-4190-9512","observed_orcids":["https://orcid.org/0000-0003-4190-9512"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shinya Shibutani","raw_affiliation_strings":["Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, NY, USA","Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, NY, USA","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]},{"raw_affiliation_string":"Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110119225","display_name":"Sung Yeon Kim","orcid":"https://orcid.org/0000-0003-4912-2554","observed_orcids":["https://orcid.org/0000-0003-4912-2554"]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sung Yeon Kim","raw_affiliation_strings":["Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100875352","display_name":"Naomi Suzuki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1327163397","display_name":"State University of New York","ror":"https://ror.org/01q1z8k08","country_code":"US","type":"education","lineage":["https://openalex.org/I1327163397"]},{"id":"https://openalex.org/I59553526","display_name":"Stony Brook University","ror":"https://ror.org/05qghxh33","country_code":"US","type":"education","lineage":["https://openalex.org/I59553526"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Naomi Suzuki","raw_affiliation_strings":["Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, NY","institution_ids":["https://openalex.org/I1327163397","https://openalex.org/I59553526"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.2353,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.94956522,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"314","issue":null,"first_page":"307","last_page":"321"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.986299991607666,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.012000000104308128,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/32p-postlabeling","display_name":"32P-postlabeling","score":0.9890000224113464},{"id":"https://openalex.org/keywords/dna-adducts","display_name":"DNA adducts","score":0.9959999918937683},{"id":"https://openalex.org/keywords/dna-damage","display_name":"DNA damage","score":0.7630000114440918},{"id":"https://openalex.org/keywords/mutagens","display_name":"mutagens","score":0.5059999823570251},{"id":"https://openalex.org/keywords/carcinogens","display_name":"carcinogens","score":0.9980000257492065},{"id":"https://openalex.org/keywords/detection-sensitivity","display_name":"detection sensitivity","score":0.6700000166893005}],"concepts":[{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.7160710692405701},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7109468579292297},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7026506066322327},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6940692067146301},{"id":"https://openalex.org/C108204754","wikidata":"https://www.wikidata.org/wiki/Q353392","display_name":"Adduct","level":2,"score":0.6013242602348328},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.5075200796127319},{"id":"https://openalex.org/C119128265","wikidata":"https://www.wikidata.org/wiki/Q900165","display_name":"Detection limit","level":2,"score":0.4349611699581146},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.4197738766670227},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2524515390396118},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002855","descriptor_name":"Chromatography, Thin Layer","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004249","descriptor_name":"DNA Damage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009705","descriptor_name":"Nucleosides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010761","descriptor_name":"Phosphorus Radioisotopes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D018736","descriptor_name":"DNA Adducts","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1385/1-59259-973-7:307","doi":"https://doi.org/10.1385/1-59259-973-7:307","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-973-7:307","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:16673890","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16673890","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3120830709","display_name":"Molecular Toxicology of DNA Adducts","funder_award_id":"P01ES004068","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"},{"id":"https://openalex.org/G5295614679","display_name":"Genotoxicity of Estrogen-and Anti-estrogen-DNA Adducts","funder_award_id":"R01ES009418","funder_id":"https://openalex.org/F4320337361","funder_display_name":"National Institute of Environmental Health Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337361","display_name":"National Institute of Environmental Health Sciences","ror":"https://ror.org/00j4k1h63"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1557623912","https://openalex.org/W1652070020","https://openalex.org/W1753558925","https://openalex.org/W1964684463","https://openalex.org/W1975166953","https://openalex.org/W1979923292","https://openalex.org/W1984771830","https://openalex.org/W2021664146","https://openalex.org/W2028753366","https://openalex.org/W2031136406","https://openalex.org/W2088055126","https://openalex.org/W2095282498","https://openalex.org/W2096227023","https://openalex.org/W2116234651","https://openalex.org/W2120782836","https://openalex.org/W2128442121","https://openalex.org/W2136007125","https://openalex.org/W2158781669"],"related_works":["https://openalex.org/W2484504859","https://openalex.org/W1975166953","https://openalex.org/W4250801280","https://openalex.org/W2063222926","https://openalex.org/W2409225759","https://openalex.org/W7220625464","https://openalex.org/W320661949","https://openalex.org/W2405424798","https://openalex.org/W2009951996","https://openalex.org/W3004354817"],"abstract_inverted_index":{"32P-Postlabeling":[0],"analysis":[1,44],"is":[2,69,116],"a":[3,81,100,138],"powerful":[4],"technique":[5],"for":[6,111,124],"detecting,":[7],"identifying,":[8],"and":[9,30,72,91,130],"quantifying":[10],"DNA":[11,25,46,60,94,115],"adducts":[12,61],"induced":[13],"by":[14,38],"mutagens":[15],"or":[16],"carcinogens.":[17],"The":[18],"method":[19],"involves":[20],"enzymatic":[21],"digestion":[22],"of":[23,33,45,114],"the":[24,34,66,108,149],"sample":[26],"to":[27,57,96,122,147,152],"nucleoside":[28],"3'-monophosphates,":[29],"partial":[31],"purification":[32],"adducted":[35],"nucleotides":[36],"followed":[37],"their":[39],"5'-labeling":[40],"with":[41,137],"32P.":[42],"For":[43,126],"adducts,":[47],"polyethyleneimine-cellulose":[48],"thin-layer":[49],"chromatography":[50,134],"(TLC)":[51],"plates":[52],"have":[53],"traditionally":[54],"been":[55],"used":[56,146],"resolve":[58],"32P-labeled":[59],"(32P-postlabeling/":[62],"TLC":[63,67],"analysis).":[64,104],"However,":[65],"procedure":[68],"time":[70],"consuming":[71],"labor":[73],"intensive.":[74],"To":[75],"expedite":[76],"analyses,":[77],"we":[78,158],"recently":[79],"devised":[80],"32P-postlabeling":[82,162],"protocol":[83],"that":[84,123],"utilizes":[85],"nondenaturing":[86],"polyacrylamide":[87],"gel":[88,102],"electrophoresis":[89],"(PAGE)":[90],"permits":[92],"multiple":[93],"samples":[95],"be":[97,145],"run":[98],"on":[99],"single":[101],"(32P-postlabeling/PAGE":[103],"Using":[105],"this":[106],"method,":[107],"detection":[109,150],"limit":[110,151],"5":[112],"microg":[113],"approx":[117,153],"7":[118],"adducts/10(9)":[119],"nucleotides,":[120],"similar":[121],"32P-postlabeling/TLC.":[125],"still":[127],"higher":[128],"sensitivity":[129],"resolution,":[131],"high-performance":[132],"liquid":[133],"(HPLC)":[135],"combined":[136],"radioisotope":[139],"detector":[140],"system":[141],"(32P-postlabeling/HPLC":[142],"analysis)":[143],"can":[144],"increase":[148],"3":[154],"adducts/10(10)":[155],"nucleotides.":[156],"Here":[157],"describe":[159],"all":[160],"three":[161],"techniques.":[163]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}