{"id":"https://openalex.org/W2396982015","doi":"https://doi.org/10.1038/srep26685","title":"Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants","display_name":"Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants","publication_year":2016,"publication_date":"2016-05-26","ids":{"openalex":"https://openalex.org/W2396982015","doi":"https://doi.org/10.1038/srep26685","mag":"2396982015","pmid":"https://pubmed.ncbi.nlm.nih.gov/27226176","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4880914"},"language":"en","primary_location":{"id":"doi:10.1038/srep26685","doi":"https://doi.org/10.1038/srep26685","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep26685","pdf_url":"https://www.nature.com/articles/srep26685.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/srep26685.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005357481","display_name":"Yuriko Osakabe","orcid":"https://orcid.org/0000-0001-5503-1427","observed_orcids":["https://orcid.org/0000-0001-5503-1427"]},"institutions":[{"id":"https://openalex.org/I4210100240","display_name":"RIKEN Center for Sustainable Resource Science","ror":"https://ror.org/010rf2m76","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210100240","https://openalex.org/I4210110652"]},{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Yuriko Osakabe","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan","RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, 305-0074, Ibaraki, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan","institution_ids":["https://openalex.org/I4210100240"]},{"raw_affiliation_string":"RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, 305-0074, Ibaraki, Japan","institution_ids":["https://openalex.org/I4210100240"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101653342","display_name":"Takahito Watanabe","orcid":"https://orcid.org/0000-0003-2978-9722","observed_orcids":["https://orcid.org/0000-0003-2978-9722"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takahito Watanabe","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038187064","display_name":"Shigeo S. Sugano","orcid":"https://orcid.org/0000-0001-9986-2839","observed_orcids":["https://orcid.org/0000-0001-9986-2839"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigeo S Sugano","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056066629","display_name":"Risa Ueta","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Risa Ueta","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075181929","display_name":"Ryosuke Ishihara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryosuke Ishihara","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105655512","display_name":"Kazuo Shinozaki","orcid":"https://orcid.org/0000-0002-6317-9867","observed_orcids":["https://orcid.org/0000-0002-6317-9867"]},"institutions":[{"id":"https://openalex.org/I4210100240","display_name":"RIKEN Center for Sustainable Resource Science","ror":"https://ror.org/010rf2m76","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210100240","https://openalex.org/I4210110652"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazuo Shinozaki","raw_affiliation_strings":["RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan","RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, 305-0074, Ibaraki, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan","institution_ids":["https://openalex.org/I4210100240"]},{"raw_affiliation_string":"RIKEN Center for Sustainable Resource Science, 3-1-1 Koyadai, Tsukuba, 305-0074, Ibaraki, Japan","institution_ids":["https://openalex.org/I4210100240"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5054315487","display_name":"Keishi Osakabe","orcid":"https://orcid.org/0000-0002-6227-6913","observed_orcids":["https://orcid.org/0000-0002-6227-6913"]},"institutions":[{"id":"https://openalex.org/I922474255","display_name":"Tokushima University","ror":"https://ror.org/044vy1d05","country_code":"JP","type":"education","lineage":["https://openalex.org/I922474255"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Keishi Osakabe","raw_affiliation_strings":["Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, Tokushima 770-8513, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Center for Collaboration among Agriculture, Industry and Commerce, Tokushima University, 3-18-15 Kuramoto-cho, 770-8503, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]},{"raw_affiliation_string":"Faculty of Bioscience and Bioindustry, Tokushima University, 2-1 Josanjima, 770-8513, Tokushima, Japan","institution_ids":["https://openalex.org/I922474255"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5005357481","https://openalex.org/A5054315487"],"corresponding_institution_ids":["https://openalex.org/I4210100240","https://openalex.org/I922474255"],"apc_list":{"value":2690,"currency":"USD","value_usd":2690},"apc_paid":{"value":2690,"currency":"USD","value_usd":2690},"fwci":13.6993,"has_fulltext":true,"cited_by_count":375,"citation_normalized_percentile":{"value":0.99343913,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"6","issue":"1","first_page":"26685","last_page":"26685"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.9769999980926514},{"id":"https://openalex.org/keywords/proton-pump","display_name":"proton pump","score":0.8149999976158142},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8970000147819519},{"id":"https://openalex.org/keywords/mutation-rate","display_name":"mutation rate","score":0.7039999961853027},{"id":"https://openalex.org/keywords/off-target-effects","display_name":"off-target effects","score":0.9729999899864197},{"id":"https://openalex.org/keywords/tissue-specific-promoters","display_name":"tissue-specific promoters","score":0.8550000190734863},{"id":"https://openalex.org/keywords/biallelic-mutations","display_name":"biallelic variants","score":0.8379999995231628},{"id":"https://openalex.org/keywords/stomatal-closure","display_name":"stomatal closure","score":0.8629999756813049},{"id":"https://openalex.org/keywords/abiotic-stress-response","display_name":"abiotic stress response","score":0.8519999980926514},{"id":"https://openalex.org/keywords/plant-molecular-breeding","display_name":"plant molecular breeding","score":0.5370000004768372}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9059630632400513},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8620713949203491},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.8473284244537354},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.700657844543457},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6727836728096008},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5978294014930725},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5322233438491821},{"id":"https://openalex.org/C98638677","wikidata":"https://www.wikidata.org/wiki/Q2067051","display_name":"Mutation rate","level":3,"score":0.527401864528656},{"id":"https://openalex.org/C89519541","wikidata":"https://www.wikidata.org/wiki/Q4667893","display_name":"Abiotic stress","level":3,"score":0.5215035080909729},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.47250616550445557},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4594363570213318},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.45730429887771606},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.4557303190231323},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27588486671447754}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D013312","descriptor_name":"Stress, Physiological","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D018745","descriptor_name":"Genome, Plant","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D030821","descriptor_name":"Plants, Genetically Modified","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1038/srep26685","doi":"https://doi.org/10.1038/srep26685","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep26685","pdf_url":"https://www.nature.com/articles/srep26685.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3862980","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4880914","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4880914","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4880914","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:27226176","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27226176","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:irdb.nii.ac.jp:00897:0005121913","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100857445","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports","raw_type":"journal article"},{"id":"pmh:oai:irdb.nii.ac.jp:00906:0006775332","doi":null,"is_oa":true,"landing_page_url":"https://tokushima-u.repo.nii.ac.jp/records/2003352","pdf_url":null,"source":{"id":"https://openalex.org/S7407056385","display_name":"Institutional Repositories DataBase (IRDB)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I184597095","host_organization_name":"National Institute of Informatics","host_organization_lineage":["https://openalex.org/I184597095"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports","raw_type":"journal article"},{"id":"pmh:oai:t2r2.star.titech.ac.jp:50587587","doi":null,"is_oa":false,"landing_page_url":"http://t2r2.star.titech.ac.jp/cgi-bin/publicationinfo.cgi?q_publication_content_number=CTT100857445","pdf_url":null,"source":{"id":"https://openalex.org/S4377196385","display_name":"Tokyo Tech Research Repository (Tokyo Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114531698","host_organization_name":"Tokyo Institute of Technology","host_organization_lineage":["https://openalex.org/I114531698"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1038/srep26685","doi":"https://doi.org/10.1038/srep26685","is_oa":true,"landing_page_url":"https://doi.org/10.1038/srep26685","pdf_url":"https://www.nature.com/articles/srep26685.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.6735000014305115,"display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1204923994","display_name":"Development of screening method using large deletion in Marchantia polymorpha","funder_award_id":"16K18472","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G4865007960","display_name":"Establishment of highly efficient chloroplast genome editing by using synthetic custom design nucleases","funder_award_id":"25450002","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G7401636891","display_name":"Genome Editing to Improve Abiotic Stress Responses in Plants","funder_award_id":"26430190","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320325854","display_name":"Council for Science, Technology and Innovation","ror":"https://ror.org/007tjaq50"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320335209","display_name":"Bio-oriented Technology Research Advancement Institution","ror":"https://ror.org/02f62sy66"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2396982015.pdf","grobid_xml":"https://content.openalex.org/works/W2396982015.grobid-xml"},"referenced_works_count":58,"referenced_works":["https://openalex.org/W994421439","https://openalex.org/W1887675732","https://openalex.org/W1937054661","https://openalex.org/W1968342623","https://openalex.org/W1980288203","https://openalex.org/W1990372437","https://openalex.org/W1990379499","https://openalex.org/W1998679905","https://openalex.org/W2000768580","https://openalex.org/W2001845502","https://openalex.org/W2002712030","https://openalex.org/W2003171404","https://openalex.org/W2011164920","https://openalex.org/W2013148539","https://openalex.org/W2017746652","https://openalex.org/W2019670881","https://openalex.org/W2021341670","https://openalex.org/W2021767455","https://openalex.org/W2026520402","https://openalex.org/W2036131768","https://openalex.org/W2040665850","https://openalex.org/W2041246911","https://openalex.org/W2045435533","https://openalex.org/W2046639072","https://openalex.org/W2047312008","https://openalex.org/W2064815984","https://openalex.org/W2073412341","https://openalex.org/W2075049721","https://openalex.org/W2076555418","https://openalex.org/W2081261052","https://openalex.org/W2084697631","https://openalex.org/W2085633386","https://openalex.org/W2090303381","https://openalex.org/W2108038398","https://openalex.org/W2110651606","https://openalex.org/W2113385887","https://openalex.org/W2115189270","https://openalex.org/W2115420193","https://openalex.org/W2117131162","https://openalex.org/W2123205135","https://openalex.org/W2123872688","https://openalex.org/W2126325519","https://openalex.org/W2127313385","https://openalex.org/W2128123767","https://openalex.org/W2131085040","https://openalex.org/W2136656609","https://openalex.org/W2146387619","https://openalex.org/W2149402050","https://openalex.org/W2152339377","https://openalex.org/W2152700648","https://openalex.org/W2153344788","https://openalex.org/W2155194596","https://openalex.org/W2157451936","https://openalex.org/W2166363131","https://openalex.org/W2167380395","https://openalex.org/W2196121581","https://openalex.org/W2510901304","https://openalex.org/W4236711181"],"related_works":["https://openalex.org/W2610236827","https://openalex.org/W2801191323","https://openalex.org/W1982802227","https://openalex.org/W2337751047","https://openalex.org/W2735521415","https://openalex.org/W2765242043","https://openalex.org/W1885181197","https://openalex.org/W2772360543","https://openalex.org/W1963554509","https://openalex.org/W2401799974"],"abstract_inverted_index":{"Genome":[0],"editing":[1,26,38],"using":[2,36,91,163],"the":[3,25,67,101,116,127,135],"CRISPR/Cas9":[4,166],"system":[5],"can":[6,130],"be":[7,29],"used":[8],"to":[9,28,85,148,159],"modify":[10],"plant":[11,33,161,165],"genomes,":[12],"however,":[13],"improvements":[14],"in":[15,22,31,55,72,98,120,126,146,156],"specificity":[16],"and":[17,66,87,112],"applicability":[18],"are":[19],"still":[20],"needed":[21],"order":[23],"for":[24,50,140],"technique":[27],"useful":[30],"various":[32],"species.":[34],"Here,":[35],"genome":[37],"mediated":[39],"by":[40,110],"a":[41,52],"truncated":[42],"gRNA":[43],"(tru-gRNA)/Cas9":[44],"combination,":[45],"we":[46],"generated":[47,70],"new":[48,136],"alleles":[49,138],"OST2,":[51],"proton":[53],"pump":[54],"Arabidopsis,":[56,121],"with":[57,78],"no":[58,88],"off-target":[59,89],"effects.":[60],"By":[61],"following":[62],"expression":[63,125],"of":[64,83],"Cas9":[65,99,107],"tru-gRNAs,":[68],"newly":[69],"mutations":[71],"CRIPSR/Cas9":[73],"transgenic":[74],"plants":[75],"were":[76],"detected":[77],"high":[79,124],"average":[80],"mutation":[81,102,118],"rates":[82],"up":[84],"32.8%":[86],"effects":[90],"constitutive":[92],"promoter.":[93],"Reducing":[94],"nuclear":[95],"localization":[96],"signals":[97],"decreased":[100],"rate.":[103],"In":[104],"contrast,":[105],"tru-gRNA":[106],"cassettes":[108],"driven":[109],"meristematic-":[111],"reproductive-tissue-specific":[113],"promoters":[114],"increased":[115],"heritable":[117],"rate":[119],"showing":[122],"that":[123],"germ":[128],"line":[129],"produce":[131],"bi-allelic":[132],"mutations.":[133],"Finally,":[134],"mutant":[137],"obtained":[139],"OST2":[141],"exhibited":[142],"altered":[143],"stomatal":[144],"closing":[145],"response":[147],"environmental":[149],"conditions.":[150],"These":[151],"results":[152],"suggest":[153],"further":[154],"applications":[155],"molecular":[157],"breeding":[158],"improve":[160],"function":[162],"optimized":[164],"systems.":[167]},"counts_by_year":[{"year":2026,"cited_by_count":24},{"year":2025,"cited_by_count":40},{"year":2024,"cited_by_count":43},{"year":2023,"cited_by_count":39},{"year":2022,"cited_by_count":39},{"year":2021,"cited_by_count":45},{"year":2020,"cited_by_count":38},{"year":2019,"cited_by_count":36},{"year":2018,"cited_by_count":39},{"year":2017,"cited_by_count":21},{"year":2016,"cited_by_count":11}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}