{"id":"https://openalex.org/W2396796069","doi":null,"title":"MAPK signaling mediates low shear stress-induced oxidative damage in human umbilical vein endothelial cells in vitro.","display_name":"MAPK signaling mediates low shear stress-induced oxidative damage in human umbilical vein endothelial cells in vitro.","publication_year":2014,"publication_date":"2014-05-01","ids":{"openalex":"https://openalex.org/W2396796069","mag":"2396796069","pmid":"https://pubmed.ncbi.nlm.nih.gov/24849420"},"language":"en","primary_location":{"id":"pmid:24849420","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24849420","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101673223","display_name":"Zhimei Wang","orcid":"https://orcid.org/0000-0002-0942-8287","observed_orcids":["https://orcid.org/0000-0002-0942-8287"]},"institutions":[{"id":"https://openalex.org/I4405277390","display_name":"Nanjing First Hospital","ror":"https://ror.org/02hw73q19","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4405277390"]},{"id":"https://openalex.org/I83519826","display_name":"Nanjing Medical University","ror":"https://ror.org/059gcgy73","country_code":"CN","type":"education","lineage":["https://openalex.org/I83519826"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhimei Wang","raw_affiliation_strings":["Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. E-mail: wzm434@126.com","Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. E-mail:"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. E-mail: wzm434@126.com","institution_ids":["https://openalex.org/I4405277390","https://openalex.org/I83519826"]},{"raw_affiliation_string":"Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China. E-mail:","institution_ids":["https://openalex.org/I4405277390","https://openalex.org/I83519826"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101895741","display_name":"Junxia Zhang","orcid":"https://orcid.org/0000-0003-1350-9001","observed_orcids":["https://orcid.org/0000-0003-1350-9001"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Junxia Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Bing Li","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bing Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034727289","display_name":"Wenxing Mao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wenxing Mao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5101609214","display_name":"Shao\u2010Liang Chen","orcid":"https://orcid.org/0000-0001-8152-564X","observed_orcids":["https://orcid.org/0000-0001-8152-564X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shaoliang Chen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2135,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.61873333,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"34","issue":"5","first_page":"603","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.9940999746322632,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12955","display_name":"Atherosclerosis and Cardiovascular Diseases","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/low-shear-stress","display_name":"low shear stress","score":0.9950000047683716},{"id":"https://openalex.org/keywords/oxidative-stress","display_name":"oxidative stress","score":0.6539999842643738},{"id":"https://openalex.org/keywords/endothelial-cells","display_name":"endothelial cells","score":0.765999972820282},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.9300000071525574},{"id":"https://openalex.org/keywords/erk","display_name":"ERK","score":0.9110000133514404},{"id":"https://openalex.org/keywords/p38-mapk","display_name":"p38 MAPK","score":0.7799999713897705},{"id":"https://openalex.org/keywords/c-jun","display_name":"c-Jun","score":0.9959999918937683},{"id":"https://openalex.org/keywords/enos-phosphorylation","display_name":"eNOS phosphorylation","score":0.7850000262260437},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.8690000176429749},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8159999847412109},{"id":"https://openalex.org/keywords/nitric-oxide","display_name":"nitric oxide","score":0.8610000014305115},{"id":"https://openalex.org/keywords/atherosclerosis","display_name":"atherosclerosis","score":0.8849999904632568},{"id":"https://openalex.org/keywords/endothelial-dysfunction","display_name":"endothelial dysfunction","score":0.7250000238418579}],"concepts":[{"id":"https://openalex.org/C2777411675","wikidata":"https://www.wikidata.org/wiki/Q1232438","display_name":"Umbilical vein","level":3,"score":0.8262532949447632},{"id":"https://openalex.org/C2778326061","wikidata":"https://www.wikidata.org/wiki/Q1761022","display_name":"Enos","level":4,"score":0.8170592784881592},{"id":"https://openalex.org/C57074206","wikidata":"https://www.wikidata.org/wiki/Q14197552","display_name":"MAPK/ERK pathway","level":3,"score":0.7488243579864502},{"id":"https://openalex.org/C51551487","wikidata":"https://www.wikidata.org/wiki/Q2043632","display_name":"p38 mitogen-activated protein kinases","level":4,"score":0.7064819931983948},{"id":"https://openalex.org/C2776151105","wikidata":"https://www.wikidata.org/wiki/Q898814","display_name":"Oxidative stress","level":2,"score":0.6393864750862122},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5498042106628418},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.48227575421333313},{"id":"https://openalex.org/C2777834122","wikidata":"https://www.wikidata.org/wiki/Q5939177","display_name":"Human umbilical vein endothelial cell","level":4,"score":0.46439889073371887},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.45667052268981934},{"id":"https://openalex.org/C519581460","wikidata":"https://www.wikidata.org/wiki/Q207843","display_name":"Nitric oxide","level":2,"score":0.45588597655296326},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4378962814807892},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.4331441819667816},{"id":"https://openalex.org/C2775838275","wikidata":"https://www.wikidata.org/wiki/Q410776","display_name":"Superoxide dismutase","level":3,"score":0.41852471232414246},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.41019344329833984},{"id":"https://openalex.org/C2777622882","wikidata":"https://www.wikidata.org/wiki/Q417619","display_name":"Nitric oxide synthase","level":3,"score":0.3581783175468445},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3350263237953186},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22451874613761902},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.163131445646286},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.13448935747146606}],"mesh":[{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005419","descriptor_name":"Flavonoids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013314","descriptor_name":"Stress, Mechanical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017382","descriptor_name":"Reactive Oxygen Species","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018384","descriptor_name":"Oxidative Stress","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020935","descriptor_name":"MAP Kinase Signaling System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D048051","descriptor_name":"p38 Mitogen-Activated Protein Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D052250","descriptor_name":"Nitric Oxide Synthase Type III","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D061307","descriptor_name":"Human Umbilical Vein Endothelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D061307","descriptor_name":"Human Umbilical Vein Endothelial Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:24849420","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24849420","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","raw_type":"Journal Article"},{"id":"mag:2396796069","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24849420","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8048999905586243}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1964740564","https://openalex.org/W1974869899","https://openalex.org/W1983246589","https://openalex.org/W2007084464","https://openalex.org/W2018344800","https://openalex.org/W2023520478","https://openalex.org/W2037581234","https://openalex.org/W2050391138","https://openalex.org/W2059694609","https://openalex.org/W2067863683","https://openalex.org/W2080336901","https://openalex.org/W2082998660","https://openalex.org/W2088624957","https://openalex.org/W2097035877","https://openalex.org/W2101746663","https://openalex.org/W2104394381","https://openalex.org/W2139139961","https://openalex.org/W2142869219","https://openalex.org/W2150703568","https://openalex.org/W2155717019","https://openalex.org/W2161387133","https://openalex.org/W2165149445","https://openalex.org/W2167060652","https://openalex.org/W2181851505"],"related_works":["https://openalex.org/W2087934989","https://openalex.org/W2038538108","https://openalex.org/W1575276508","https://openalex.org/W2181851505","https://openalex.org/W2561954457","https://openalex.org/W2145413661","https://openalex.org/W2075676654","https://openalex.org/W2616466497","https://openalex.org/W2017972491","https://openalex.org/W2803082166"],"abstract_inverted_index":{"OBJECTIVE:":[0],"Atherosclerotic":[1],"lesions":[2],"occur":[3],"preferentially":[4],"in":[5,25,32,99,114,147,154,228],"the":[6,21,79,110,118,131,160],"arterial":[7],"branches,":[8],"bifurcations":[9],"and":[10,63,69,92,125,128,152,166,184],"curvatures":[11],"where":[12],"shear":[13,27],"stress":[14,28],"is":[15,211],"low.":[16],"We":[17],"aimed":[18],"to":[19,47,77,141,214,224],"study":[20],"possible":[22],"mechanisms":[23],"involved":[24,113],"low":[26],"(LSS)-induced":[29],"oxidative":[30,116,209],"damage":[31,210],"vascular":[33],"endothelial":[34,40,82,229],"cells.":[35,230],"METHODS:":[36],"Human":[37],"umbilical":[38],"vein":[39],"cells":[41,100,119],"(HUVECs)":[42],"exposed":[43],"for":[44,57,104,130,143],"60":[45],"min":[46],"simulated":[48],"LSS":[49,102,142,156],"using":[50],"a":[51],"parallel-plate":[52],"flow":[53],"chamber":[54],"were":[55,120],"examined":[56,129],"intracellular":[58],"reactive":[59],"oxygen":[60],"species":[61],"(ROS)":[62],"cell":[64],"apoptosis":[65,153],"with":[66,101,122,180,196,200],"chemiluminescence":[67],"assay":[68],"TUNEL":[70],"staining,":[71],"respectively.":[72],"Western":[73],"blotting":[74],"was":[75],"used":[76],"determine":[78],"levels":[80],"of":[81,133,162,178,193],"nitric":[83],"oxide":[84,187],"synthase":[85],"(eNOS),":[86],"P38,":[87,123,164],"extracellular":[88],"signal-regulate":[89],"kinase":[90],"(ERK)":[91],"c-Jun":[93,126,167,199],"as":[94,96],"well":[95],"their":[97,173],"phosphorylation":[98,161],"exposure":[103,157],"different":[105],"time":[106],"lengths.":[107],"To":[108],"investigate":[109],"signaling":[111],"pathway":[112],"LSS-induced":[115,208],"damage,":[117],"treated":[121],"ERK":[124,165,179,222],"inhibitors":[127],"expression":[132],"eNOS-Thr495":[134,183],"that":[135],"negatively":[136],"regulated":[137],"eNOS.":[138],"RESULTS:":[139],"Exposure":[140],"1":[144],"h":[145],"resulted":[146],"markedly":[148],"increased":[149],"ROS":[150],"accumulation":[151],"HUVECs.":[155],"time-dependently":[158],"enhanced":[159],"eNOS,":[163],"but":[168],"did":[169,202],"not":[170],"significantly":[171],"affect":[172],"total":[174],"protein":[175,217],"expressions.":[176],"Inhibition":[177],"PD98059":[181],"deactivated":[182],"restored":[185],"super":[186],"dismutase":[188],"(SOD)":[189],"activity,":[190],"while":[191],"inhibition":[192],"either":[194],"p38":[195],"SB202190":[197],"or":[198],"SP600125":[201],"no":[203],"produce":[204],"such":[205],"effects.":[206],"CONCLUSION:":[207],"partly":[212],"due":[213],"activated":[215],"mitogen-activated":[216],"kinases":[218],"(MAPK),":[219],"among":[220],"which":[221],"contributes":[223],"decreased":[225],"NO":[226],"release":[227]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}