{"id":"https://openalex.org/W2396632119","doi":"https://doi.org/10.1016/s0091-679x(08)61618-0","title":"Chapter 18 Fractionation of Yeast Organelles","display_name":"Chapter 18 Fractionation of Yeast Organelles","publication_year":1989,"publication_date":"1989-01-01","ids":{"openalex":"https://openalex.org/W2396632119","doi":"https://doi.org/10.1016/s0091-679x(08)61618-0","mag":"2396632119","pmid":"https://pubmed.ncbi.nlm.nih.gov/2674627"},"language":"en","primary_location":{"id":"doi:10.1016/s0091-679x(08)61618-0","doi":"https://doi.org/10.1016/s0091-679x(08)61618-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0091-679x(08)61618-0","pdf_url":null,"source":{"id":"https://openalex.org/S177811526","display_name":"Methods in cell biology","issn_l":"0091-679X","issn":["0091-679X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"book series"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Cell Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4244273924","display_name":null},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0091-679x(08)61618-0","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059633854","display_name":"Nancy C. Walworth","orcid":"https://orcid.org/0000-0001-5940-8236","observed_orcids":["https://orcid.org/0000-0001-5940-8236"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nancy C. Walworth","raw_affiliation_strings":["Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024297903","display_name":"Bruno Goud","orcid":"https://orcid.org/0000-0003-1227-4159","observed_orcids":["https://orcid.org/0000-0003-1227-4159"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bruno Goud","raw_affiliation_strings":["Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011432367","display_name":"Hannele Ruohola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hannele Ruohola","raw_affiliation_strings":["Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028536518","display_name":"Peter Novick","orcid":"https://orcid.org/0000-0003-0401-7857","observed_orcids":["https://orcid.org/0000-0003-0401-7857"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter J. Novick","raw_affiliation_strings":["Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology Yale University School of Medicine New Haven, Connecticut 06510","institution_ids":["https://openalex.org/I32971472"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I32971472"],"apc_list":null,"apc_paid":null,"fwci":4.7781,"has_fulltext":false,"cited_by_count":57,"citation_normalized_percentile":{"value":0.97472924,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":96},"biblio":{"volume":"31","issue":null,"first_page":"335","last_page":"354"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.8955000042915344,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.8955000042915344,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.09749999642372131,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13127","display_name":"Biotin and Related Studies","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/secretory-pathway","display_name":"secretory pathway","score":0.9750000238418579},{"id":"https://openalex.org/keywords/differential-centrifugation","display_name":"differential centrifugation","score":0.8289999961853027},{"id":"https://openalex.org/keywords/sucrose-gradient-fractionation","display_name":"sucrose gradient fractionation","score":0.9929999709129333},{"id":"https://openalex.org/keywords/secretory-vesicles","display_name":"secretory vesicles","score":0.8579999804496765},{"id":"https://openalex.org/keywords/protein-localization","display_name":"protein localization","score":0.7289999723434448},{"id":"https://openalex.org/keywords/membrane-protein-isolation","display_name":"membrane protein isolation","score":0.3619999885559082}],"concepts":[{"id":"https://openalex.org/C168240541","wikidata":"https://www.wikidata.org/wiki/Q45948","display_name":"Organelle","level":2,"score":0.8823344111442566},{"id":"https://openalex.org/C119062480","wikidata":"https://www.wikidata.org/wiki/Q83181","display_name":"Golgi apparatus","level":3,"score":0.7718071937561035},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.7712147831916809},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7501815557479858},{"id":"https://openalex.org/C27239569","wikidata":"https://www.wikidata.org/wiki/Q189029","display_name":"Cell fractionation","level":3,"score":0.7183729410171509},{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.5312297940254211},{"id":"https://openalex.org/C6492254","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretory pathway","level":4,"score":0.5190268754959106},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5029692053794861},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5021111965179443},{"id":"https://openalex.org/C2909863526","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretory Vesicle","level":4,"score":0.4623042643070221},{"id":"https://openalex.org/C97428945","wikidata":"https://www.wikidata.org/wiki/Q15305109","display_name":"Fractionation","level":2,"score":0.4484829008579254},{"id":"https://openalex.org/C156399914","wikidata":"https://www.wikidata.org/wiki/Q7444454","display_name":"Secretory protein","level":3,"score":0.44555899500846863},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4242631196975708},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3753186762332916},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.35132062435150146},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.349544882774353},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.20382484793663025},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.14252007007598877}],"mesh":[{"descriptor_ui":"D002458","descriptor_name":"Cell Fractionation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002499","descriptor_name":"Centrifugation, Density Gradient","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005656","descriptor_name":"Fungal Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007202","descriptor_name":"Indicators and Reagents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D013104","descriptor_name":"Spheroplasts","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015388","descriptor_name":"Organelles","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0091-679x(08)61618-0","doi":"https://doi.org/10.1016/s0091-679x(08)61618-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0091-679x(08)61618-0","pdf_url":null,"source":{"id":"https://openalex.org/S177811526","display_name":"Methods in cell biology","issn_l":"0091-679X","issn":["0091-679X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"book series"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Cell Biology","raw_type":"book-chapter"},{"id":"pmid:2674627","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2674627","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in cell biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0091-679x(08)61618-0","doi":"https://doi.org/10.1016/s0091-679x(08)61618-0","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0091-679x(08)61618-0","pdf_url":null,"source":{"id":"https://openalex.org/S177811526","display_name":"Methods in cell biology","issn_l":"0091-679X","issn":["0091-679X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"book series"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Cell Biology","raw_type":"book-chapter"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G33749474","display_name":"Predoctoral Program in Cellular and Molecular Biology","funder_award_id":"T32GM007223","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3993375212","display_name":null,"funder_award_id":"GM 35370","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W146070251","https://openalex.org/W193459211","https://openalex.org/W627044590","https://openalex.org/W1481175115","https://openalex.org/W1503233261","https://openalex.org/W1553938468","https://openalex.org/W1748010957","https://openalex.org/W1966446749","https://openalex.org/W1991938470","https://openalex.org/W1994815981","https://openalex.org/W2006943139","https://openalex.org/W2013026243","https://openalex.org/W2015571616","https://openalex.org/W2016417523","https://openalex.org/W2037398430","https://openalex.org/W2059775037","https://openalex.org/W2063959590","https://openalex.org/W2065852288","https://openalex.org/W2073073066","https://openalex.org/W2075142049","https://openalex.org/W2087298783","https://openalex.org/W2090486711","https://openalex.org/W2092514617","https://openalex.org/W2093303213","https://openalex.org/W2104229396","https://openalex.org/W2116133156","https://openalex.org/W2133449590","https://openalex.org/W2139467741","https://openalex.org/W2144990328","https://openalex.org/W2323037227"],"related_works":["https://openalex.org/W1991938470","https://openalex.org/W74260879","https://openalex.org/W1602150101","https://openalex.org/W1552339094","https://openalex.org/W1538592956","https://openalex.org/W2065852288","https://openalex.org/W1747506719","https://openalex.org/W2056486352","https://openalex.org/W2124428264","https://openalex.org/W2175663749"],"abstract_inverted_index":{"In":[0],"summary,":[1],"organelles":[2,49,127,170,203],"of":[3,23,34,43,47,59,67,80,91,100,107,125,154,166,202,223,239,249],"the":[4,35,44,60,75,97,101,104,111,123,129,152,164,171,182,200,216,221,235,247],"secretory":[5,69,155],"pathway":[6],"can":[7,71,229],"be":[8,72,207,230],"effectively":[9],"separated":[10],"from":[11,74,157,199],"one":[12,51],"another":[13],"using":[14,241],"differential":[15],"centrifugation":[16],"followed":[17],"by":[18],"sucrose":[19],"density":[20],"gradient":[21],"fractionation":[22],"wild-type":[24],"or":[25],"vesicle-accumulating":[26],"mutant":[27],"yeast":[28,158],"cells.":[29,114],"Up":[30],"to":[31,52,56,96,168,173,211],"10-fold":[32],"enrichment":[33],"plasma":[36,133],"membrane":[37,78,134,228],"fraction":[38,79],"is":[39,94,177,244],"obtained,":[40],"and":[41,103,136,139,142,256],"resolution":[42],"peak":[45],"fractions":[46],"several":[48],"allows":[50],"localize":[53],"specific":[54],"proteins":[55,167,198],"particular":[57,169],"components":[58],"pathway.":[61],"Additionally,":[62],"a":[63,85,224,227],"highly":[64],"purified":[65],"population":[66],"constitutive":[68],"vesicles":[70,102,108,156],"isolated":[73],"100,000":[76],"g":[77],"sec":[81,112],"6-4":[82,113],"cells":[83,175],"on":[84,234],"Sephacryl":[86],"S-1000":[87],"column.":[88],"The":[89,187],"success":[90],"this":[92],"procedure":[93,150],"due":[95],"homogeneous":[98],"size":[99],"high":[105],"concentration":[106],"accumulated":[109],"in":[110],"From":[115],"other":[116,126],"laboratories,":[117],"methods":[118,260],"have":[119],"been":[120],"described":[121],"for":[122,151,219,261],"isolation":[124],"including":[128],"vacuole":[130],"(Wiemken,":[131],"1975),":[132],"(Tschopp":[135],"Schekman,":[137],"1983),":[138],"nuclei":[140],"(Mann":[141],"Mecke,":[143],"1980),":[144],"as":[145,147,252],"well":[146],"an":[148,178],"alternative":[149,242],"purification":[153],"(Holcomb":[159],"et":[160],"al.,":[161],"1987).":[162],"For":[163],"localization":[165,240],"ability":[172],"lyse":[174],"osmotically":[176],"important":[179],"improvement":[180],"over":[181],"glass":[183,192],"bead":[184,193],"lysis":[185,194,236],"procedure.":[186],"shear":[188],"forces":[189],"generated":[190],"during":[191],"could":[195],"potentially":[196],"remove":[197],"surface":[201],"that":[204],"otherwise":[205],"would":[206],"membrane-attached,":[208],"causing":[209],"them":[210],"appear":[212],"soluble.":[213],"Similarly,":[214],"because":[215],"conditions":[217],"required":[218],"stabilizing":[220],"association":[222],"protein":[225],"with":[226],"quite":[231],"variable":[232],"depending":[233],"buffer,":[237],"confirmation":[238],"schemes":[243],"prudent.":[245],"With":[246],"advent":[248],"such":[250],"techniques":[251],"confocal":[253],"immunofluorescent":[254],"microscopy":[255],"immunoelectron":[257],"microscopy,":[258],"effective":[259],"confirming":[262],"localizations":[263],"are":[264],"becoming":[265],"available.":[266]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}
