{"id":"https://openalex.org/W2396611085","doi":null,"title":"[Construction and identification of matrix metalloproteinase inhibitor-1 siRNA eukaryotic expression vectors].","display_name":"[Construction and identification of matrix metalloproteinase inhibitor-1 siRNA eukaryotic expression vectors].","publication_year":2007,"publication_date":"2007-03-01","ids":{"openalex":"https://openalex.org/W2396611085","mag":"2396611085","pmid":"https://pubmed.ncbi.nlm.nih.gov/17407710"},"language":"zh","primary_location":{"id":"pmid:17407710","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17407710","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology","raw_type":"English Abstract"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100345592","display_name":"Tian Li","orcid":"https://orcid.org/0000-0003-4362-0130","observed_orcids":["https://orcid.org/0000-0003-4362-0130"]},"institutions":[{"id":"https://openalex.org/I4210156804","display_name":"First Affiliated Hospital of Zhengzhou University","ror":"https://ror.org/056swr059","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210156804"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Tian","raw_affiliation_strings":["Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. tianyu615999@sina.com","Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. tianyu615999@sina.com","institution_ids":["https://openalex.org/I4210156804"]},{"raw_affiliation_string":"Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China","institution_ids":["https://openalex.org/I4210156804"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043528779","display_name":"Jichang Li","orcid":"https://orcid.org/0000-0002-1779-1190","observed_orcids":["https://orcid.org/0000-0002-1779-1190"]},"institutions":[{"id":"https://openalex.org/I38877650","display_name":"Zhengzhou University","ror":"https://ror.org/04ypx8c21","country_code":"CN","type":"education","lineage":["https://openalex.org/I38877650"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ji-chang Li","raw_affiliation_strings":["450052,\u90d1\u5dde\u5927\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\u6d88\u5316\u5185\u79d1"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"450052,\u90d1\u5dde\u5927\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\u6d88\u5316\u5185\u79d1","institution_ids":["https://openalex.org/I38877650"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106121036","display_name":"Guo-qiang Zhao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I38877650","display_name":"Zhengzhou University","ror":"https://ror.org/04ypx8c21","country_code":"CN","type":"education","lineage":["https://openalex.org/I38877650"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guo-qiang Zhao","raw_affiliation_strings":["\u90d1\u5dde\u5927\u5b66\u533b\u5b66\u9662\u5fae\u751f\u7269\u514d\u75ab\u6559\u7814\u5ba4"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u90d1\u5dde\u5927\u5b66\u533b\u5b66\u9662\u5fae\u751f\u7269\u514d\u75ab\u6559\u7814\u5ba4","institution_ids":["https://openalex.org/I38877650"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101517653","display_name":"Kuisheng Chen","orcid":"https://orcid.org/0000-0002-0500-5543","observed_orcids":["https://orcid.org/0000-0002-0500-5543"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kui-sheng Chen","raw_affiliation_strings":["\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100961157","display_name":"Wen-hui Fan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I38877650","display_name":"Zhengzhou University","ror":"https://ror.org/04ypx8c21","country_code":"CN","type":"education","lineage":["https://openalex.org/I38877650"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen-hui Fan","raw_affiliation_strings":["\u90d1\u5dde\u5927\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\u5916\u79d1"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u90d1\u5dde\u5927\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\u5916\u79d1","institution_ids":["https://openalex.org/I38877650"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xin Lou","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xin Lou","raw_affiliation_strings":["\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Miao-miao Sun","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Miao-miao Sun","raw_affiliation_strings":["\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111904697","display_name":"Xuequan Cao","orcid":"https://orcid.org/0000-0002-6554-2041","observed_orcids":["https://orcid.org/0000-0002-6554-2041"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xue-quan Cao","raw_affiliation_strings":["\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u6cb3\u5357\u7701\u80bf\u7624\u75c5\u7406\u91cd\u70b9\u5b9e\u9a8c\u5ba4","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.3235647,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"3","first_page":"196","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.7979000210762024,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.7979000210762024,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.15710000693798065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10966","display_name":"Liver physiology and pathology","score":0.019099999219179153,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/timp-1","display_name":"TIMP-1","score":0.8930000066757202},{"id":"https://openalex.org/keywords/sirna","display_name":"siRNA","score":0.9399999976158142},{"id":"https://openalex.org/keywords/eukaryotic-expression-vector","display_name":"eukaryotic expression vector","score":0.921999990940094},{"id":"https://openalex.org/keywords/hepatic-stellate-cells","display_name":"hepatic stellate cells","score":0.9509999752044678},{"id":"https://openalex.org/keywords/gene-silencing","display_name":"gene silencing","score":0.7450000047683716},{"id":"https://openalex.org/keywords/timp-1-mrna-expression","display_name":"TIMP-1 mRNA expression","score":0.8529999852180481}],"concepts":[{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7849304676055908},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7023704051971436},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6384353637695312},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5718852281570435},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5240036249160767},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4940526783466339},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.47290170192718506},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4311107397079468},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4188881814479828},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3005516827106476},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13883823156356812}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019715","descriptor_name":"Tissue Inhibitor of Metalloproteinase-1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055166","descriptor_name":"Hepatic Stellate Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:17407710","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17407710","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology","raw_type":"English Abstract"},{"id":"mag:2396611085","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17407710","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2417406464","https://openalex.org/W2389696583","https://openalex.org/W2977316817","https://openalex.org/W2382757946","https://openalex.org/W2348280026","https://openalex.org/W2389468391","https://openalex.org/W2353347474","https://openalex.org/W65832471","https://openalex.org/W2359612071","https://openalex.org/W2393287360"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"construct":[2],"TIMP-1":[3,13,42,82,93,100,117,130],"siRNA":[4,94,118],"eukaryotic":[5,95,119],"expression":[6,15,84,96,102,120],"vectors":[7,97,121],"and":[8,31,45,50,55,68,122],"evaluate":[9],"their":[10],"effect":[11],"on":[12],"mRNA":[14,83,101],"in":[16],"hepatic":[17],"stellate":[18],"cells.":[19],"METHODS:":[20],"The":[21],"combinant":[22],"lone":[23],"DNA":[24,69],"with":[25],"cutting":[26],"sites":[27],"of":[28,39,43,92],"BamH":[29],"I":[30,33],"Xho":[32],"enzyme":[34,65],"according":[35],"to":[36,52,57],"the":[37,110,123,129],"sequences":[38,91],"447-465,":[40],"552-540":[41],"rats":[44],"nonspecific":[46],"sequence":[47],"were":[48,60,73,98],"selected":[49],"cloned":[51],"pGEM-T":[53],"vector":[54],"sub-cloned":[56],"pRNAT-U6.2.":[58],"They":[59],"then":[61],"identified":[62],"by":[63,87,106],"double":[64],"digestion":[66],"analysis":[67],"sequencing.":[70],"Three":[71],"plasmids":[72],"transfected":[74,124],"into":[75,109],"T6":[76],"separately":[77],"through":[78],"an":[79],"oligofectamine":[80],"package.":[81],"was":[85,103],"evaluated":[86],"RT-PCR.":[88],"RESULTS:":[89],"Targeting":[90],"correct.":[99],"significantly":[104,127],"reduced":[105],"transfecting":[107],"them":[108],"T6.":[111],"CONCLUSION:":[112],"We":[113],"successfully":[114],"constructed":[115],"two":[116],"cells":[125],"can":[126],"suppress":[128],"expression.":[131]},"counts_by_year":[],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}
