{"id":"https://openalex.org/W2396076691","doi":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","title":"Bombesin stimulation of gastrin release from canine gastrin cells in primary culture","display_name":"Bombesin stimulation of gastrin release from canine gastrin cells in primary culture","publication_year":1987,"publication_date":"1987-03-01","ids":{"openalex":"https://openalex.org/W2396076691","doi":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","mag":"2396076691","pmid":"https://pubmed.ncbi.nlm.nih.gov/2435171"},"language":"en","primary_location":{"id":"doi:10.1152/ajpgi.1987.252.3.g413","doi":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","pdf_url":null,"source":{"id":"https://openalex.org/S34136372","display_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","issn_l":"0193-1857","issn":["0193-1857","1522-1547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320155","host_organization_name":"American Physiological Society","host_organization_lineage":["https://openalex.org/P4310320155"],"host_organization_lineage_names":["American Physiological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061588591","display_name":"A. S. Giraud","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. S. Giraud","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083325211","display_name":"Andrew H. Soll","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. H. Soll","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012428409","display_name":"Frank F. Cuttitta","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F. Cuttitta","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091680206","display_name":"John Henry Walsh","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J. H. Walsh","raw_affiliation_strings":[""],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.7785,"has_fulltext":false,"cited_by_count":64,"citation_normalized_percentile":{"value":0.91864439,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"252","issue":"3","first_page":"G413","last_page":"G420"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10814","display_name":"Neuropeptides and Animal Physiology","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bombesin","display_name":"bombesin","score":0.9869999885559082},{"id":"https://openalex.org/keywords/gastrin-release","display_name":"gastrin release","score":0.9430000185966492},{"id":"https://openalex.org/keywords/gastrin-cells","display_name":"gastrin cells","score":0.9150000214576721},{"id":"https://openalex.org/keywords/somatostatin","display_name":"somatostatin","score":0.847000002861023},{"id":"https://openalex.org/keywords/substance-p","display_name":"substance P","score":0.8240000009536743},{"id":"https://openalex.org/keywords/spantide","display_name":"spantide","score":0.9150000214576721},{"id":"https://openalex.org/keywords/bombesin-receptor","display_name":"bombesin receptor","score":0.777999997138977},{"id":"https://openalex.org/keywords/somatostatin-receptors","display_name":"somatostatin receptors","score":0.9459999799728394},{"id":"https://openalex.org/keywords/adenylate-cyclase","display_name":"adenylate cyclase","score":0.8650000095367432},{"id":"https://openalex.org/keywords/camp","display_name":"cyclic AMP","score":0.6520000100135803},{"id":"https://openalex.org/keywords/protein-kinase-c","display_name":"protein kinase C","score":0.9679999947547913},{"id":"https://openalex.org/keywords/calcium-ionophore","display_name":"calcium ionophore","score":0.6930000185966492},{"id":"https://openalex.org/keywords/primary-culture","display_name":"primary cell culture","score":0.5910000205039978},{"id":"https://openalex.org/keywords/canine-stomach","display_name":"canine stomach","score":0.5799999833106995}],"concepts":[{"id":"https://openalex.org/C2778448772","wikidata":"https://www.wikidata.org/wiki/Q349427","display_name":"Bombesin","level":4,"score":0.9534010887145996},{"id":"https://openalex.org/C2776986829","wikidata":"https://www.wikidata.org/wiki/Q407181","display_name":"Gastrin","level":3,"score":0.8279176950454712},{"id":"https://openalex.org/C2776297358","wikidata":"https://www.wikidata.org/wiki/Q407166","display_name":"Somatostatin","level":2,"score":0.7814770936965942},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.730631947517395},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.6871184706687927},{"id":"https://openalex.org/C119811328","wikidata":"https://www.wikidata.org/wiki/Q2987004","display_name":"G cell","level":4,"score":0.6379820108413696},{"id":"https://openalex.org/C2779276759","wikidata":"https://www.wikidata.org/wiki/Q412747","display_name":"Forskolin","level":3,"score":0.4820204973220825},{"id":"https://openalex.org/C195794163","wikidata":"https://www.wikidata.org/wiki/Q420877","display_name":"Protein kinase C","level":3,"score":0.4726628065109253},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.45472729206085205},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4279562532901764},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37257498502731323},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.3207702040672302},{"id":"https://openalex.org/C118303440","wikidata":"https://www.wikidata.org/wiki/Q419968","display_name":"Neuropeptide","level":3,"score":0.22330361604690552},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.17608654499053955},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1709454357624054},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.16854330897331238},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10283252596855164}],"mesh":[{"descriptor_ui":"D000001","descriptor_name":"Calcimycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000262","descriptor_name":"Adenylyl Cyclases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001839","descriptor_name":"Bombesin","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D001839","descriptor_name":"Bombesin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004285","descriptor_name":"Dogs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005576","descriptor_name":"Colforsin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005750","descriptor_name":"Gastric Juice","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005753","descriptor_name":"Gastric Mucosa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005755","descriptor_name":"Gastrins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011493","descriptor_name":"Protein Kinase C","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012636","descriptor_name":"Secretory Rate","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D013004","descriptor_name":"Somatostatin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013373","descriptor_name":"Substance P","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D013373","descriptor_name":"Substance P","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013755","descriptor_name":"Tetradecanoylphorbol Acetate","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017981","descriptor_name":"Receptors, Neurotransmitter","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D018004","descriptor_name":"Receptors, Bombesin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1152/ajpgi.1987.252.3.g413","doi":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","is_oa":false,"landing_page_url":"https://doi.org/10.1152/ajpgi.1987.252.3.g413","pdf_url":null,"source":{"id":"https://openalex.org/S34136372","display_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","issn_l":"0193-1857","issn":["0193-1857","1522-1547"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320155","host_organization_name":"American Physiological Society","host_organization_lineage":["https://openalex.org/P4310320155"],"host_organization_lineage_names":["American Physiological Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Physiology-Gastrointestinal and Liver Physiology","raw_type":"journal-article"},{"id":"pmid:2435171","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2435171","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The American journal of physiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G6488019569","display_name":"HORMONAL STIMULATION AND INHIBITION OF GASTRIC SECRETION","funder_award_id":"5r01am017294-12","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6576530867","display_name":"PHYSIOLOGY OF THE ISOLATED MAMMALIAN PARIETAL CELL","funder_award_id":"5r01am019984-09","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G8244367770","display_name":null,"funder_award_id":"AM 17 328","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2132363825","https://openalex.org/W2001869437","https://openalex.org/W1635391868","https://openalex.org/W2137943961","https://openalex.org/W2064743262","https://openalex.org/W2283603477","https://openalex.org/W2077404915","https://openalex.org/W2238806621","https://openalex.org/W2274733394","https://openalex.org/W2180637202"],"abstract_inverted_index":{"A":[0],"method":[1],"is":[2],"described":[3],"for":[4,29],"the":[5,85,127],"isolation":[6],"and":[7,21,27,38,121,149,161,184],"short-term":[8,150],"culture":[9,151],"of":[10,116,122,152],"canine":[11],"antral":[12,153,174],"gastrin":[13,51,77,97,112,132,140],"(G)":[14],"cells.":[15,45,176],"Tissue":[16],"was":[17],"dispersed":[18],"by":[19,25,87,119,126,182],"enzymes":[20],"G":[22,36,154,175,178],"cells":[23,37,157,179],"enriched":[24],"elutriation":[26],"cultured":[28],"40":[30],"h.":[31,62],"These":[32],"cultures":[33,55],"contained":[34],"12%":[35],"less":[39],"than":[40],"2%":[41],"somatostatin-":[42],"or":[43,95],"serotonin-containing":[44],"Bombesin":[46],"(0.001-100":[47],"pM)":[48],"potently":[49],"stimulated":[50],"release":[52],"from":[53],"cell":[54],"in":[56,141],"a":[57,142,170],"linear":[58],"fashion":[59],"over":[60],"2":[61],"The":[63,99,134],"bombesin-specific":[64],"monoclonal":[65],"antibody":[66],"2A11":[67],"dose-dependently":[68],"blocked":[69],"bombesin":[70,160],"stimulation.":[71],"Somatostatin":[72],"(0.001-1,000":[73],"nM)":[74],"inhibited":[75],"bombesin-stimulated":[76,94,111],"release.":[78,98,113,133],"Antibody":[79],"to":[80],"somatostatin":[81,89,163],"(Mab":[82],"S8)":[83],"prevented":[84],"inhibition":[86],"exogenous":[88],"but":[90],"did":[91,108],"not":[92,109],"alter":[93],"basal":[96],"substance":[100],"P":[101],"(SP)":[102],"analogue":[103],"spantide":[104],"(1":[105],"nM-1":[106],"microM)":[107],"inhibit":[110],"Postreceptor":[114],"activation":[115],"adenylate":[117],"cyclase":[118],"forskolin":[120],"protein":[123],"kinase":[124],"C":[125],"phorbol":[128],"ester,":[129],"beta-TPA,":[130],"caused":[131],"calcium":[135],"ionophore":[136],"A23187":[137],"also":[138],"released":[139],"dose-dependent":[143],"fashion.":[144],"This":[145],"methodology":[146],"allows":[147],"enrichment":[148],"cells;":[155],"these":[156,167],"have":[158,169],"stimulatory":[159],"inhibitory":[162],"receptors,":[164],"suggesting":[165],"that":[166],"peptides":[168],"direct":[171],"action":[172],"on":[173],"Furthermore,":[177],"are":[180],"activated":[181],"cAMP":[183],"calcium/phosphatidylinositol-dependent":[185],"mechanisms.":[186]},"counts_by_year":[{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}