{"id":"https://openalex.org/W2395916269","doi":null,"title":"Release of nucleosomes from nuclei by bleomycin-induced DNA strand scission.","display_name":"Release of nucleosomes from nuclei by bleomycin-induced DNA strand scission.","publication_year":1989,"publication_date":"1989-05-01","ids":{"openalex":"https://openalex.org/W2395916269","mag":"2395916269","pmid":"https://pubmed.ncbi.nlm.nih.gov/2476991"},"language":"en","primary_location":{"id":"pmid:2476991","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2476991","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry international","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112793659","display_name":"K Hamana","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165735259","display_name":"Gunma University","ror":"https://ror.org/046fm7598","country_code":"JP","type":"education","lineage":["https://openalex.org/I165735259"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K Hamana","raw_affiliation_strings":["College of Medical Care and Technology, Gunma University, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Medical Care and Technology, Gunma University, Japan","institution_ids":["https://openalex.org/I165735259"]}]},{"author_position":"last","author":{"id":null,"display_name":"K Kawada","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Kawada","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"18","issue":"5","first_page":"971","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.8784000277519226,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.8784000277519226,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.039799999445676804,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.025100000202655792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bleomycin","display_name":"bleomycin","score":0.9789999723434448},{"id":"https://openalex.org/keywords/dna-strand-scission","display_name":"DNA strand scission","score":0.9860000014305115},{"id":"https://openalex.org/keywords/chromatin","display_name":"chromatin","score":0.7310000061988831},{"id":"https://openalex.org/keywords/hmg1","display_name":"HMG1","score":0.7400000095367432},{"id":"https://openalex.org/keywords/hmg2","display_name":"HMG2","score":0.9980000257492065},{"id":"https://openalex.org/keywords/histone-h1","display_name":"histone H1","score":0.8379999995231628},{"id":"https://openalex.org/keywords/linker-dna","display_name":"linker DNA","score":0.7749999761581421},{"id":"https://openalex.org/keywords/tetrahymena","display_name":"Tetrahymena","score":0.7250000238418579},{"id":"https://openalex.org/keywords/micrococcal-nuclease","display_name":"staphylococcal nuclease","score":0.8190000057220459},{"id":"https://openalex.org/keywords/dna-cleavage","display_name":"DNA cleavage","score":0.5529999732971191},{"id":"https://openalex.org/keywords/polyamines","display_name":"polyamines","score":0.6389999985694885},{"id":"https://openalex.org/keywords/chelating-agents","display_name":"chelating agents","score":0.9279999732971191},{"id":"https://openalex.org/keywords/free-radical-scavengers","display_name":"radical scavengers","score":0.8939999938011169}],"concepts":[{"id":"https://openalex.org/C83640560","wikidata":"https://www.wikidata.org/wiki/Q180951","display_name":"Chromatin","level":3,"score":0.7391263246536255},{"id":"https://openalex.org/C139024627","wikidata":"https://www.wikidata.org/wiki/Q4293132","display_name":"Micrococcal nuclease","level":5,"score":0.6844720840454102},{"id":"https://openalex.org/C64927066","wikidata":"https://www.wikidata.org/wiki/Q36293","display_name":"Histone","level":3,"score":0.6558260917663574},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6484339237213135},{"id":"https://openalex.org/C84772758","wikidata":"https://www.wikidata.org/wiki/Q271794","display_name":"Nucleosome","level":4,"score":0.6299039721488953},{"id":"https://openalex.org/C101152365","wikidata":"https://www.wikidata.org/wiki/Q838135","display_name":"Histone H1","level":4,"score":0.512227475643158},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5010263919830322},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4714142978191376},{"id":"https://openalex.org/C2776232574","wikidata":"https://www.wikidata.org/wiki/Q415571","display_name":"Bleomycin","level":3,"score":0.4547225832939148},{"id":"https://openalex.org/C2780835560","wikidata":"https://www.wikidata.org/wiki/Q5449573","display_name":"Protamine","level":3,"score":0.4247826933860779},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4139402508735657},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3820043206214905},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34185367822647095},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11453619599342346},{"id":"https://openalex.org/C2777557582","wikidata":"https://www.wikidata.org/wiki/Q190016","display_name":"Heparin","level":2,"score":0.0},{"id":"https://openalex.org/C2776694085","wikidata":"https://www.wikidata.org/wiki/Q974135","display_name":"Chemotherapy","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001761","descriptor_name":"Bleomycin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002499","descriptor_name":"Centrifugation, Density Gradient","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003851","descriptor_name":"Deoxyribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D004249","descriptor_name":"DNA Damage","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004587","descriptor_name":"Electrophoresis, Agar Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007502","descriptor_name":"Iron Chelating Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D009707","descriptor_name":"Nucleosomes","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D010618","descriptor_name":"Phenanthrolines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013768","descriptor_name":"Tetrahymena","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014337","descriptor_name":"Trout","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:2476991","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2476991","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry international","raw_type":"Comparative Study"},{"id":"mag:2395916269","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2476991","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1971927260","https://openalex.org/W2061946335","https://openalex.org/W2075965014","https://openalex.org/W1796206844","https://openalex.org/W2973498831","https://openalex.org/W2151207998","https://openalex.org/W1592023228","https://openalex.org/W2014532072","https://openalex.org/W1566053834","https://openalex.org/W2015643019"],"abstract_inverted_index":{"Mononucleosomes":[0],"were":[1,26,60,77,94,109],"released":[2,35],"from":[3,28,36,112],"both":[4,90],"isolated":[5],"mammalian":[6],"(hog":[7],"thyroid)":[8],"and":[9,47,51,92,107,152,157,188],"protozoan":[10],"(Tetrahymena)":[11],"nuclei":[12,39,102,172],"by":[13,66,82,99,147],"the":[14,23,29,37,41,57,63,80,119,123,128,135,159,165],"bleomycin-induced":[15,168],"DNA-strand":[16],"breaking":[17],"reaction.":[18],"Trout":[19],"sperm":[20],"nuclei,":[21],"on":[22,167],"other":[24],"hand,":[25],"protected":[27],"bleomycin-mediated":[30],"DNA":[31,86,124,143,160,169],"degradation.":[32],"The":[33,162],"mononucleosomes":[34,64],"bleomycin-treated":[38],"contained":[40],"core":[42,58],"histones":[43],"H2A,":[44],"H2B,":[45],"H3,":[46],"H4;":[48],"while":[49],"HMG1":[50,106],"HMG2":[52,108],"proteins,":[53],"in":[54,62,96,150],"addition":[55],"to":[56,141],"histones,":[59],"detected":[61],"obtained":[65],"micrococcal":[67,104],"nuclease":[68],"digestion":[69,100],"of":[70,84,101,127,164,171,173],"nuclei.":[71],"HMGs,":[72],"but":[73],"not":[74,145],"H1":[75,93,148],"histone,":[76],"dissociated":[78,111],"into":[79],"supernatant":[81],"cleavage":[83,170],"chromatin":[85,113,151],"with":[87,103,114],"bleomycin,":[88],"whereas":[89],"HMGs":[91],"found":[95],"that":[97,137],"fraction":[98],"nuclease.":[105],"exclusively":[110,153],"1":[115],"mM":[116],"bleomycin":[117,138],"under":[118],"solvent":[120],"condition":[121],"where":[122],"strand-breaking":[125],"activity":[126],"drug":[129],"is":[130],"repressed.":[131],"These":[132],"observations":[133],"suggest":[134],"possibility":[136],"preferentially":[139],"binds":[140],"linker":[142],"regions":[144],"occupied":[146],"histone":[149],"dissociates":[154],"HMG":[155],"proteins":[156],"breaks":[158],"strand.":[161],"results":[163],"effects":[166],"various":[174],"drugs":[175],"including":[176],"polyamines,":[177],"chelating":[178],"agents,":[179],"intercalating":[180],"antibiotics":[181],"such":[182],"as":[183],"mitomycin":[184],"C":[185],"or":[186],"adriamycin,":[187],"radical":[189],"scavengers":[190],"are":[191],"also":[192],"presented.":[193]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}