{"id":"https://openalex.org/W2395500217","doi":"https://doi.org/10.1385/1-59259-079-9:189","title":"Advantages and Disadvantages of Multiple Different Methods of Adenoviral Vector Construction","display_name":"Advantages and Disadvantages of Multiple Different Methods of Adenoviral Vector Construction","publication_year":2003,"publication_date":"2003-11-14","ids":{"openalex":"https://openalex.org/W2395500217","doi":"https://doi.org/10.1385/1-59259-079-9:189","mag":"2395500217","pmid":"https://pubmed.ncbi.nlm.nih.gov/21341057"},"language":"en","primary_location":{"id":"doi:10.1385/1-59259-079-9:189","doi":"https://doi.org/10.1385/1-59259-079-9:189","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-079-9:189","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatocellular Carcinoma","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4239390271","display_name":"Hepatocellular Carcinoma"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108423789","display_name":"Prem K. Seth","orcid":"https://orcid.org/0000-0001-6292-2121","observed_orcids":["https://orcid.org/0000-0001-6292-2121"]},"institutions":[{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Prem Seth","raw_affiliation_strings":["Human Gene Therapy Research Center, Des Moines, IA","Human Gene Therapy Research Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Gene Therapy Research Center, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]},{"raw_affiliation_string":"Human Gene Therapy Research Center","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005791249","display_name":"James N. Higginbotham","orcid":"https://orcid.org/0000-0002-1496-7998","observed_orcids":["https://orcid.org/0000-0002-1496-7998"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210140884","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James Higginbotham","raw_affiliation_strings":["National Cancer Institute, National Institutes of Health, Bethesda, MD","National Cancer Institute, National Institutes of Health"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Cancer Institute, National Institutes of Health, Bethesda, MD","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210140884"]},{"raw_affiliation_string":"National Cancer Institute, National Institutes of Health","institution_ids":["https://openalex.org/I4210140884"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.666,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.69183673,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"45","issue":null,"first_page":"189","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adenovirus-vectors","display_name":"adenoviral vector","score":0.9919999837875366},{"id":"https://openalex.org/keywords/adenovirus-serotypes","display_name":"adenovirus serotypes","score":0.8740000128746033},{"id":"https://openalex.org/keywords/adenovirus-genome","display_name":"adenovirus genome","score":0.5870000123977661},{"id":"https://openalex.org/keywords/viral-transcription","display_name":"viral transcription","score":0.47200000286102295},{"id":"https://openalex.org/keywords/viral-dna-replication","display_name":"viral DNA replication","score":0.8130000233650208},{"id":"https://openalex.org/keywords/adenovirus-e1a","display_name":"adenovirus E1A","score":0.6729999780654907},{"id":"https://openalex.org/keywords/e1b","display_name":"E1B","score":0.9959999918937683},{"id":"https://openalex.org/keywords/e3-region","display_name":"E3 region","score":0.6840000152587891},{"id":"https://openalex.org/keywords/early-and-late-gene-expression","display_name":"early and late gene expression","score":0.6679999828338623},{"id":"https://openalex.org/keywords/viral-packaging","display_name":"viral packaging","score":0.4620000123977661},{"id":"https://openalex.org/keywords/adenovirus-type-5","display_name":"adenovirus type 5","score":0.6129999756813049},{"id":"https://openalex.org/keywords/gene-therapy-vectors","display_name":"gene therapy vectors","score":0.4880000054836273}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6829521059989929},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.6111900210380554},{"id":"https://openalex.org/C73573662","wikidata":"https://www.wikidata.org/wiki/Q130996","display_name":"DNA replication","level":3,"score":0.5529982447624207},{"id":"https://openalex.org/C140704245","wikidata":"https://www.wikidata.org/wiki/Q3933202","display_name":"Viral replication","level":3,"score":0.520456075668335},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5163654088973999},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5103523135185242},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.464485228061676},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.46230024099349976},{"id":"https://openalex.org/C151158763","wikidata":"https://www.wikidata.org/wiki/Q1626856","display_name":"Origin recognition complex","level":5,"score":0.4302116334438324},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.42298567295074463},{"id":"https://openalex.org/C2776954459","wikidata":"https://www.wikidata.org/wiki/Q193447","display_name":"Adenoviridae","level":4,"score":0.41869693994522095},{"id":"https://openalex.org/C104690221","wikidata":"https://www.wikidata.org/wiki/Q5165872","display_name":"Control of chromosome duplication","level":4,"score":0.4178480803966522},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.3765736520290375},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3674529194831848},{"id":"https://openalex.org/C98086919","wikidata":"https://www.wikidata.org/wiki/Q118019","display_name":"Eukaryotic DNA replication","level":4,"score":0.3079802393913269},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2904239892959595},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.17372554540634155},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1385/1-59259-079-9:189","doi":"https://doi.org/10.1385/1-59259-079-9:189","is_oa":false,"landing_page_url":"https://doi.org/10.1385/1-59259-079-9:189","pdf_url":null,"source":{"id":"https://openalex.org/S4306463399","display_name":"Humana Press eBooks","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310315801","host_organization_name":"Humana Press","host_organization_lineage":["https://openalex.org/P4310315801","https://openalex.org/P4310319900"],"host_organization_lineage_names":["Humana Press","Springer Science+Business Media"],"type":"ebook platform"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hepatocellular Carcinoma","raw_type":"book-chapter"},{"id":"pmid:21341057","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21341057","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular medicine","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W302957210","https://openalex.org/W1965295123","https://openalex.org/W1965518509","https://openalex.org/W1980401440","https://openalex.org/W2003066004","https://openalex.org/W2015111081","https://openalex.org/W2016200465","https://openalex.org/W2026544727","https://openalex.org/W2033798265","https://openalex.org/W2037230449","https://openalex.org/W2039040205","https://openalex.org/W2044296969","https://openalex.org/W2044629552","https://openalex.org/W2065354528","https://openalex.org/W2075921029","https://openalex.org/W2081825650","https://openalex.org/W2133916339","https://openalex.org/W2139041386","https://openalex.org/W2142807370","https://openalex.org/W2158744232","https://openalex.org/W2419332336"],"related_works":["https://openalex.org/W2080991301","https://openalex.org/W197737109","https://openalex.org/W2153452606","https://openalex.org/W2040318657","https://openalex.org/W2024948923","https://openalex.org/W2734836672","https://openalex.org/W97471962","https://openalex.org/W197877330","https://openalex.org/W1608020969","https://openalex.org/W2213939923"],"abstract_inverted_index":{"Adenoviruses":[0,24],"are":[1,60],"medium-sized":[2],"nonenveloped":[3],"DNA":[4,14,115,125,216,221],"tumor":[5],"viruses":[6],"whose":[7],"genome":[8,105],"consists":[9],"of":[10,16,31,50,88,102,124,132,140,171],"a":[11,29],"linear":[12],"double-stranded":[13],"molecule":[15],"about":[17],"36":[18],"Kb":[19],"(reviewed":[20],"in":[21,85],"refs.":[22],"1-3).":[23],"have":[25,45,78],"icosahedral":[26],"geometry":[27],"with":[28,48,65,91],"diameter":[30],"approx":[32],"70-100":[33],"nm.":[34],"Currently,":[35],"over":[36],"100":[37],"different":[38],"mammalian":[39],"and":[40,63,76,82,93,98,117,148,165,191,223],"foul":[41],"adenovirus":[42],"(Ad)":[43],"serotypes":[44,59],"been":[46,79],"characterized,":[47],"49":[49],"them":[51],"being":[52,68],"human":[53],"serotypes.":[54],"The":[55,206],"most":[56,70],"extensively":[57,80],"characterized":[58],"2,":[61],"5,":[62],"12,":[64],"type-5-based":[66],"vectors":[67],"the":[69,86,103,122,129,136,172,179,196,200],"popular.":[71],"Ad":[72,104],"virus":[73],"transcription,":[74],"replication,":[75,116],"packaging":[77],"studied":[81],"take":[83],"place":[84],"nucleus":[87],"infected":[89],"cells,":[90],"transcription":[92],"replication":[94],"requiring":[95],"both":[96],"cellular":[97,188],"viral":[99,186,215],"proteins.":[100],"Expression":[101],"is":[106,157],"divided":[107,159],"into":[108,160],"two":[109,161],"phases:":[110],"early,":[111],"which":[112,119,150],"occurs":[113,120],"before":[114],"late,":[118],"after":[121],"initiation":[123],"replication.":[126],"Transcription":[127],"during":[128],"early":[130,180],"phase":[131],"adenoviral":[133],"infection":[134],"involves":[135],"highly":[137],"orchestrated":[138],"expression":[139],"four":[141],"pol":[142],"IIdependent":[143],"regions,":[144],"E1,":[145],"E2,":[146],"E3,":[147],"E4,":[149],"each":[151],"code":[152],"for":[153,210,214],"multiple":[154],"mRNAs.":[155],"E1":[156],"further":[158],"transcriptional":[162,169],"units":[163],"E1A":[164,167],"E1B.":[166],"coordinates":[168],"control":[170],"virus,":[173],"whereas":[174],"E1B":[175,197],"gene":[176],"products":[177],"influence":[178],"to":[181,187],"late":[182],"transition":[183],"by":[184,204],"coordinating":[185],"mRNA":[189],"metabolism":[190],"host":[192],"protein":[193,219],"shutoff.":[194],"Additionally,":[195],"region":[198,208],"obstructs":[199],"apoptotic":[201],"signals":[202],"induced":[203],"E1A.":[205],"E2":[207],"codes":[209],"three":[211],"proteins":[212],"essential":[213],"replication;":[217],"terminal":[218],"precursor,":[220],"polymerase,":[222],"DNA.":[224]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2}],"updated_date":"2026-10-10T09:11:00.788812","created_date":"2016-06-24T00:00:00"}