{"id":"https://openalex.org/W2394792511","doi":null,"title":"Alterations in cyclic AMP phosphodiesterase activities during differentiation of 3T3-L1 cells.","display_name":"Alterations in cyclic AMP phosphodiesterase activities during differentiation of 3T3-L1 cells.","publication_year":1983,"publication_date":"1983-01-01","ids":{"openalex":"https://openalex.org/W2394792511","mag":"2394792511","pmid":"https://pubmed.ncbi.nlm.nih.gov/6196386"},"language":"en","primary_location":{"id":"pmid:6196386","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6196386","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cyclic nucleotide and protein phosphorylation research","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005295670","display_name":"Manganiello Vc","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V C Manganiello","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027584417","display_name":"Reed Bc","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B C Reed","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014264452","display_name":"Frank Scott Lieberman","orcid":"https://orcid.org/0000-0003-2577-3336","observed_orcids":["https://orcid.org/0000-0003-2577-3336"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F S Lieberman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"J Moss","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J Moss","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110122895","display_name":"Lan\u0115","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M D Lane","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5102796390","display_name":"Malcolm J. Vaughan","orcid":"https://orcid.org/0009-0006-0645-6510","observed_orcids":["https://orcid.org/0009-0006-0645-6510"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Vaughan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4006,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.54857229,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"9","issue":"2","first_page":"143","last_page":"54"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12434","display_name":"Phosphodiesterase function and regulation","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11072","display_name":"Peroxisome Proliferator-Activated Receptors","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyclic-amp-phosphodiesterase","display_name":"cyclic AMP phosphodiesterase","score":0.9800000190734863},{"id":"https://openalex.org/keywords/cyclic-gmp-phosphodiesterase","display_name":"cyclic GMP phosphodiesterase","score":0.9679999947547913},{"id":"https://openalex.org/keywords/3t3-l1-cells","display_name":"3T3-L1 cells","score":0.9430000185966492},{"id":"https://openalex.org/keywords/adipogenic-differentiation","display_name":"adipogenesis","score":0.9829999804496765},{"id":"https://openalex.org/keywords/particulate-fraction","display_name":"particulate fraction","score":0.7049999833106995},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5389999747276306},{"id":"https://openalex.org/keywords/substrate-inhibition","display_name":"substrate inhibition","score":0.6940000057220459},{"id":"https://openalex.org/keywords/enzyme-solubilization","display_name":"enzyme solubilization","score":0.6209999918937683}],"concepts":[{"id":"https://openalex.org/C62826618","wikidata":"https://www.wikidata.org/wiki/Q424304","display_name":"Phosphodiesterase","level":3,"score":0.9031473398208618},{"id":"https://openalex.org/C24245907","wikidata":"https://www.wikidata.org/wiki/Q498957","display_name":"Particulates","level":2,"score":0.5431524515151978},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5288516283035278},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4970081150531769},{"id":"https://openalex.org/C2780215583","wikidata":"https://www.wikidata.org/wiki/Q946700","display_name":"Cyclic nucleotide phosphodiesterase","level":4,"score":0.41895946860313416},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.412385493516922},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32259124517440796},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000273","descriptor_name":"Adipose Tissue","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D000273","descriptor_name":"Adipose Tissue","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002147","descriptor_name":"Calmodulin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003907","descriptor_name":"Dexamethasone","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007328","descriptor_name":"Insulin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015056","descriptor_name":"1-Methyl-3-isobutylxanthine","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015105","descriptor_name":"3',5'-Cyclic-AMP Phosphodiesterases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015106","descriptor_name":"3',5'-Cyclic-GMP Phosphodiesterases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:6196386","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6196386","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of cyclic nucleotide and protein phosphorylation research","raw_type":"Journal Article"},{"id":"mag:2394792511","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/6196386","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1548037071","https://openalex.org/W1978092737","https://openalex.org/W1804469564","https://openalex.org/W2966197633","https://openalex.org/W1535151692","https://openalex.org/W2035499918","https://openalex.org/W1883085811","https://openalex.org/W2409701072","https://openalex.org/W1601120844","https://openalex.org/W2007897250"],"abstract_inverted_index":{"3T3-L1":[0,33,136],"cells":[1,27,113,203],"contain":[2],"multiple":[3],"forms":[4],"of":[5,39,55,68,105,153,177,201,228,236],"cyclic":[6],"nucleotide":[7],"phosphodiesterase":[8,43,90,109,131,190,195,229,239,247],"in":[9,47,78,81,85,111,114,119,129,187,225,249],"both":[10,23],"supernatant":[11,87],"(100,000":[12],"X":[13],"g,":[14],"40":[15],"min)":[16],"and":[17,25,51,72,94,158,173,193,207],"particulate":[18,49,57,70,99,130,164,188,199,215,238,246],"fractions.":[19,216],"Supernatant":[20],"fractions":[21,134,165],"from":[22,135,166,198,205],"undifferentiated":[24,32,120,167],"differentiated":[26,112,202],"contained":[28],"calmodulin-sensitive":[29],"activity.":[30,132,191],"In":[31,122,133,163],"cells,":[34,168],"only":[35],"a":[36,75,126,184,237,245],"small":[37],"fraction":[38,50,100,200],"the":[40,48,52,56,61,65,69,86,106,213,226,234],"total":[41,79,88,107],"cAMP":[42,89,108,140,161,192],"activity":[44,54,67,80,91,96,110,230],"was":[45,58,74,125,182],"found":[46,248],"specific":[53,66,95],"lower":[59],"than":[60,155],"supernatant.":[62],"With":[63,179],"differentiation":[64],"doubled,":[71],"there":[73,124,181,219],"dramatic":[76],"increase":[77,186],"this":[82],"fraction,":[83],"while":[84],"increased":[92],"less":[93,154],"decreased.":[97],"The":[98],"accounted":[101],"for":[102],"approximately":[103],"70%":[104],"contrast":[115],"to":[116,212,221,244],"about":[117],"one-third":[118],"cells.":[121],"addition,":[123],"qualitative":[127],"change":[128],"adipocytes,":[137],"with":[138,149,240],"either":[139],"or":[141],"cGMP":[142,159,169,189,194],"as":[143,231,233],"substrate,":[144],"Lineweaver-Burk":[145],"plots":[146],"were":[147],"nonlinear,":[148],"low":[150],"Km":[151],"components":[152],"1":[156],"microM,":[157],"inhibited":[160],"hydrolysis.":[162],"did":[170],"not":[171],"inhibit":[172],"often":[174],"enhanced":[175],"hydrolysis":[176],"cAMP.":[178],"differentiation,":[180,218],"also":[183],"marked":[185],"activities":[196],"solubilized":[197],"coeluted":[204],"DEAE-Biogel":[206],"exhibited":[208],"kinetic":[209,241],"properties":[210,242],"similar":[211,243],"crude":[214],"During":[217],"seems":[220],"be":[222],"an":[223],"alteration":[224],"distribution":[227],"well":[232],"appearance":[235],"mature":[250],"rat":[251],"adipocytes.":[252]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}