{"id":"https://openalex.org/W2394642004","doi":null,"title":"Comparison of two chemical cleavage methods for preparation of a truncated form of recombinant human insulin-like growth factor I from a secreted fusion protein.","display_name":"Comparison of two chemical cleavage methods for preparation of a truncated form of recombinant human insulin-like growth factor I from a secreted fusion protein.","publication_year":1989,"publication_date":"1989-12-01","ids":{"openalex":"https://openalex.org/W2394642004","mag":"2394642004","pmid":"https://pubmed.ncbi.nlm.nih.gov/2696476"},"language":"en","primary_location":{"id":"pmid:2696476","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2696476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioFactors (Oxford, England)","raw_type":"Comparative Study"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113803683","display_name":"G\u00f6ran Forsberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G Forsberg","raw_affiliation_strings":["KubiGen AB, Stockholm, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"KubiGen AB, Stockholm, Sweden","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004556365","display_name":"Barbro Baastrup","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"B Baastrup","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038413035","display_name":"Michael Brobjer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Brobjer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111633823","display_name":"M. Lake","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Lake","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038289136","display_name":"Hans J\u00f6rnvall","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H J\u00f6rnvall","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5108391311","display_name":"Maris G. N. Hartmanis","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Hartmanis","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.1846,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.79147023,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"2","issue":"2","first_page":"105","last_page":"12"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6845999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10748","display_name":"Growth Hormone and Insulin-like Growth Factors","score":0.2870999872684479,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.007000000216066837,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insulin-like-growth-factor-i","display_name":"insulin-like growth factor I","score":0.9959999918937683},{"id":"https://openalex.org/keywords/recombinant-protein","display_name":"recombinant protein","score":0.7229999899864197},{"id":"https://openalex.org/keywords/fusion-protein","display_name":"fusion protein","score":0.9129999876022339},{"id":"https://openalex.org/keywords/chemical-cleavage","display_name":"chemical cleavage","score":0.8949999809265137},{"id":"https://openalex.org/keywords/n-chlorosuccinimide","display_name":"N-chlorosuccinimide","score":0.9259999990463257},{"id":"https://openalex.org/keywords/cyanogen-bromide","display_name":"cyanogen bromide","score":0.9950000047683716},{"id":"https://openalex.org/keywords/methionine-oxidation","display_name":"methionine oxidation","score":0.8389999866485596},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.8240000009536743},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.5360000133514404},{"id":"https://openalex.org/keywords/bioactivity","display_name":"biological activity","score":0.6589999794960022},{"id":"https://openalex.org/keywords/proteolysis","display_name":"proteolysis","score":0.8220000267028809}],"concepts":[{"id":"https://openalex.org/C2777093645","wikidata":"https://www.wikidata.org/wiki/Q420258","display_name":"Cyanogen bromide","level":4,"score":0.878453254699707},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.7625472545623779},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.7275355458259583},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6140719652175903},{"id":"https://openalex.org/C2780912031","wikidata":"https://www.wikidata.org/wiki/Q180341","display_name":"Methionine","level":3,"score":0.6001331806182861},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5772896409034729},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5558317303657532},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5260909795761108},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5072112083435059},{"id":"https://openalex.org/C14053116","wikidata":"https://www.wikidata.org/wiki/Q284398","display_name":"N-terminus","level":4,"score":0.48765480518341064},{"id":"https://openalex.org/C2777568990","wikidata":"https://www.wikidata.org/wiki/Q6823656","display_name":"Methionine sulfoxide","level":4,"score":0.487643837928772},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.46155688166618347},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.41542887687683105},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.41370639204978943},{"id":"https://openalex.org/C2780557392","wikidata":"https://www.wikidata.org/wiki/Q523796","display_name":"Linker","level":2,"score":0.41119423508644104},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.39340460300445557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2831602096557617},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16336190700531006},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.08017387986183167},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005285","descriptor_name":"Fermentation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007334","descriptor_name":"Insulin-Like Growth Factor I","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D007334","descriptor_name":"Insulin-Like Growth Factor I","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007525","descriptor_name":"Isoelectric Focusing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008715","descriptor_name":"Methionine","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011869","descriptor_name":"Radioligand Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013002","descriptor_name":"Somatomedins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:2696476","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2696476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioFactors (Oxford, England)","raw_type":"Comparative Study"},{"id":"mag:2394642004","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2696476","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.428600013256073,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1961182819","https://openalex.org/W1980506664","https://openalex.org/W1823351566","https://openalex.org/W2020027636","https://openalex.org/W97951211","https://openalex.org/W2049471699","https://openalex.org/W2020497113","https://openalex.org/W3030105539","https://openalex.org/W2045024488","https://openalex.org/W2045547097"],"abstract_inverted_index":{"We":[0],"have":[1],"produced":[2],"a":[3,27,37,73,77,109,153],"naturally":[4],"occurring":[5],"variant":[6],"of":[7,159,170,185,194],"human":[8],"insulin-like":[9],"growth":[10,86],"factor":[11],"I,":[12],"truncated":[13,171],"by":[14],"three":[15],"amino":[16,20],"acids":[17],"at":[18,71],"the":[19,43,49,63,81,85,101,105,113,120],"terminus.":[21],"The":[22,33,122,149],"polypeptide":[23],"is":[24,36,46,52],"obtained":[25],"as":[26,181],"fusion":[28,34,44,64,82],"protein":[29,45,65],"in":[30,111],"Escherichia":[31],"coli.":[32],"partner":[35,83],"synthetic":[38],"IgG-binding":[39],"peptide.":[40],"During":[41],"fermentation":[42,200],"secreted":[47],"into":[48],"medium,":[50],"and":[51,84,131,146,177,183,201],"purified":[53,150,176],"on":[54],"IgG--Sepharose":[55],"prior":[56],"to":[57,119,136,157],"cleavage.":[58,203],"Two":[59],"different":[60],"genes":[61],"for":[62,187],"were":[66,132,173],"used,":[67],"allowing":[68],"chemical":[69,202],"cleavage":[70,99,107,126,139],"either":[72],"tryptophan":[74],"linker":[75,79],"or":[76,91],"methionine":[78,115,188,195],"between":[80],"factor,":[87],"using":[88],"N-chlorosuccinimide":[89],"(NCS)":[90],"cyanogen":[92],"bromide":[93],"(CNBr)":[94],"respectively.":[95],"A":[96],"partial":[97],"CNBr":[98],"yielded":[100,108],"native":[102],"peptide,":[103],"whereas":[104],"NCS":[106],"product":[110,151],"which":[112],"single":[114],"had":[116,152],"been":[117],"oxidized":[118],"sulfoxide.":[121],"forms":[123,169],"from":[124,162],"both":[125,199],"methods":[127,140],"exhibited":[128],"biological":[129,154],"activity":[130,155],"characterized":[133],"after":[134],"purification":[135],"homogeneity.":[137],"Both":[138],"gave":[141],"products":[142],"having":[143],"correct":[144],"N-":[145],"C-terminal":[147],"ends.":[148],"equal":[156],"that":[158],"corresponding":[160],"material":[161],"natural":[163],"sources,":[164],"15":[165],"000":[166],"U/mg.":[167],"Modified":[168],"IGF-I":[172],"also":[174],"identified,":[175],"characterized.":[178],"Modifications":[179],"such":[180],"proteolysis":[182],"misincorporation":[184],"norleucine":[186],"occurred":[189],"during":[190,198],"biosynthesis,":[191],"while":[192],"oxidation":[193],"took":[196],"place":[197]},"counts_by_year":[{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}