{"id":"https://openalex.org/W2394621867","doi":"https://doi.org/10.1089/hgtb.2015.013","title":"Systematic Comparison and Validation of Quantitative Real-Time PCR Methods for the Quantitation of Adeno-Associated Viral Products","display_name":"Systematic Comparison and Validation of Quantitative Real-Time PCR Methods for the Quantitation of Adeno-Associated Viral Products","publication_year":2015,"publication_date":"2015-05-08","ids":{"openalex":"https://openalex.org/W2394621867","doi":"https://doi.org/10.1089/hgtb.2015.013","mag":"2394621867","pmid":"https://pubmed.ncbi.nlm.nih.gov/25953194","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4492554"},"language":"en","primary_location":{"id":"doi:10.1089/hgtb.2015.013","doi":"https://doi.org/10.1089/hgtb.2015.013","is_oa":false,"landing_page_url":"https://doi.org/10.1089/hgtb.2015.013","pdf_url":null,"source":{"id":"https://openalex.org/S2495496942","display_name":"Human Gene Therapy Methods","issn_l":"1946-6536","issn":["1946-6536","1946-6544"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","poland-70"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Gene Therapy Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4492554","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044432975","display_name":"Natalie Jayne Werling","orcid":"https://orcid.org/0000-0001-5813-6128","observed_orcids":["https://orcid.org/0000-0001-5813-6128"]},"institutions":[{"id":"https://openalex.org/I4405282191","display_name":"Medicines and Healthcare products Regulatory Agency","ror":"https://ror.org/05fan1e59","country_code":"GB","type":"government","lineage":["https://openalex.org/I4405282191"]},{"id":"https://openalex.org/I65163767","display_name":"National Institute for Biological Standards and Control","ror":"https://ror.org/03dnc6n82","country_code":"GB","type":"facility","lineage":["https://openalex.org/I65163767"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Natalie Jayne Werling","raw_affiliation_strings":["Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom","institution_ids":["https://openalex.org/I4405282191","https://openalex.org/I65163767"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018374489","display_name":"Stifani Satkunanathan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405282191","display_name":"Medicines and Healthcare products Regulatory Agency","ror":"https://ror.org/05fan1e59","country_code":"GB","type":"government","lineage":["https://openalex.org/I4405282191"]},{"id":"https://openalex.org/I65163767","display_name":"National Institute for Biological Standards and Control","ror":"https://ror.org/03dnc6n82","country_code":"GB","type":"facility","lineage":["https://openalex.org/I65163767"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Stifani Satkunanathan","raw_affiliation_strings":["Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom","institution_ids":["https://openalex.org/I4405282191","https://openalex.org/I65163767"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013830579","display_name":"Robin Thorpe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405282191","display_name":"Medicines and Healthcare products Regulatory Agency","ror":"https://ror.org/05fan1e59","country_code":"GB","type":"government","lineage":["https://openalex.org/I4405282191"]},{"id":"https://openalex.org/I65163767","display_name":"National Institute for Biological Standards and Control","ror":"https://ror.org/03dnc6n82","country_code":"GB","type":"facility","lineage":["https://openalex.org/I65163767"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Robin Thorpe","raw_affiliation_strings":["Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom","institution_ids":["https://openalex.org/I4405282191","https://openalex.org/I65163767"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5071454359","display_name":"Yuan Zhao","orcid":"https://orcid.org/0000-0003-3523-9751","observed_orcids":["https://orcid.org/0000-0003-3523-9751","https://orcid.org/0000-0002-9945-0575"]},"institutions":[{"id":"https://openalex.org/I4405282191","display_name":"Medicines and Healthcare products Regulatory Agency","ror":"https://ror.org/05fan1e59","country_code":"GB","type":"government","lineage":["https://openalex.org/I4405282191"]},{"id":"https://openalex.org/I65163767","display_name":"National Institute for Biological Standards and Control","ror":"https://ror.org/03dnc6n82","country_code":"GB","type":"facility","lineage":["https://openalex.org/I65163767"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Yuan Zhao","raw_affiliation_strings":["Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Advanced Therapies, NIBSC/Medicines and Healthcare Products Regulatory Agency, Potters Bar, Hertfordshire, EN6 3QG United Kingdom","institution_ids":["https://openalex.org/I4405282191","https://openalex.org/I65163767"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5071454359"],"corresponding_institution_ids":["https://openalex.org/I4405282191","https://openalex.org/I65163767"],"apc_list":null,"apc_paid":null,"fwci":1.2421,"has_fulltext":false,"cited_by_count":54,"citation_normalized_percentile":{"value":0.80295076,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"26","issue":"3","first_page":"82","last_page":"92"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9199000000953674,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9199000000953674,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.07240000367164612,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adeno-associated-virus","display_name":"adeno-associated virus","score":0.8320000171661377},{"id":"https://openalex.org/keywords/qpcr-assay-validation","display_name":"qPCR assay validation","score":0.6230000257492065},{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.8169999718666077},{"id":"https://openalex.org/keywords/sample-preparation","display_name":"sample preparation","score":0.9359999895095825},{"id":"https://openalex.org/keywords/reference-materials","display_name":"reference materials","score":0.6269999742507935},{"id":"https://openalex.org/keywords/gene-therapy-vectors","display_name":"gene therapy vectors","score":0.828000009059906},{"id":"https://openalex.org/keywords/assay-standardization","display_name":"assay standardization","score":0.6610000133514404}],"concepts":[{"id":"https://openalex.org/C2778107364","wikidata":"https://www.wikidata.org/wiki/Q355989","display_name":"Adeno-associated virus","level":5,"score":0.6371837854385376},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.5967214107513428},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5758887529373169},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3935261368751526},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3824763298034668},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3795310854911804},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.27835696935653687},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1448032557964325},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12278622388839722},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.07061675190925598},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000229","descriptor_name":"Dependovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000229","descriptor_name":"Dependovirus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1089/hgtb.2015.013","doi":"https://doi.org/10.1089/hgtb.2015.013","is_oa":false,"landing_page_url":"https://doi.org/10.1089/hgtb.2015.013","pdf_url":null,"source":{"id":"https://openalex.org/S2495496942","display_name":"Human Gene Therapy Methods","issn_l":"1946-6536","issn":["1946-6536","1946-6544"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","poland-70"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human Gene Therapy Methods","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4492554","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4492554","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hum Gene Ther Methods","raw_type":"Text"},{"id":"pmid:25953194","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25953194","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Human gene therapy methods","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.814.4220","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.814.4220","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/60/48/hgtb.2015.013.PMC4492554.pdf","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:4492554","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4492554","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Hum Gene Ther Methods","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320319990","display_name":"National Institute for Health and Care Research","ror":"https://ror.org/0187kwz08"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1943985333","https://openalex.org/W1967200408","https://openalex.org/W1968143805","https://openalex.org/W1971008570","https://openalex.org/W1990559406","https://openalex.org/W1994030719","https://openalex.org/W2001599454","https://openalex.org/W2010015867","https://openalex.org/W2012098470","https://openalex.org/W2020108708","https://openalex.org/W2022161423","https://openalex.org/W2026505509","https://openalex.org/W2026878553","https://openalex.org/W2035671670","https://openalex.org/W2042765141","https://openalex.org/W2051159468","https://openalex.org/W2052621353","https://openalex.org/W2055777720","https://openalex.org/W2058942457","https://openalex.org/W2065241845","https://openalex.org/W2069895701","https://openalex.org/W2076356087","https://openalex.org/W2079090019","https://openalex.org/W2121944814","https://openalex.org/W2165891422","https://openalex.org/W2168420558","https://openalex.org/W2180590412","https://openalex.org/W2231194414","https://openalex.org/W4245087155"],"related_works":["https://openalex.org/W7096973881","https://openalex.org/W2962334628","https://openalex.org/W3153049993","https://openalex.org/W2076735302","https://openalex.org/W3089684647","https://openalex.org/W3126886085","https://openalex.org/W2947717893","https://openalex.org/W2567596901","https://openalex.org/W2916895573","https://openalex.org/W4385752239"],"abstract_inverted_index":{"Adeno-associated":[0],"viral":[1],"(AAV)":[2],"vectors":[3],"show":[4],"great":[5],"promise":[6],"for":[7,22,94],"gene":[8],"therapy":[9],"because":[10],"of":[11,18,33,38,48,57,74,82,101],"their":[12],"excellent":[13],"safety":[14],"profile;":[15],"however,":[16],"development":[17],"robust":[19],"dose-determining":[20],"assays":[21],"AAV":[23,39,75],"has":[24],"presented":[25],"a":[26],"significant":[27],"challenge.":[28],"With":[29],"the":[30,46,55,71,80,92],"ultimate":[31],"goal":[32],"future":[34],"harmonization":[35],"and":[36,59,66,86,90,99],"standardization":[37],"dose":[40,106],"determination":[41],"assays,":[42],"we":[43],"systematically":[44],"analyzed":[45],"influence":[47],"key":[49],"variables,":[50],"including":[51],"sample":[52,88],"preparation":[53,89],"procedure,":[54],"choice":[56],"primers,":[58],"real-time":[60],"quantitative":[61],"PCR":[62],"(qPCR)":[63],"target":[64],"sequences":[65],"calibration":[67],"DNA":[68],"conformation":[69],"on":[70],"qPCR":[72,84],"quantitation":[73],"products.":[76],"Our":[77],"results":[78],"emphasize":[79],"importance":[81],"designing":[83],"primers":[85],"conducting":[87],"demonstrate":[91],"need":[93],"extensive":[95],"characterization,":[96],"vigorous":[97],"control,":[98],"use":[100],"reference":[102],"materials":[103],"in":[104],"clinical":[105],"determination.":[107]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":12},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}