{"id":"https://openalex.org/W2392228588","doi":null,"title":"Subtyping of H1N1,H1N2 and H3N2 swine influenza viruses by two multiplex RT-PCR","display_name":"Subtyping of H1N1,H1N2 and H3N2 swine influenza viruses by two multiplex RT-PCR","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2392228588","mag":"2392228588"},"language":"en","primary_location":{"id":"mag:2392228588","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/article_en/cjfdtotal-zgxq200709012.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764717153","display_name":"Zhongguo yufang shouyi xuebao","issn_l":"1008-0589","issn":["1008-0589"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696733","host_organization_name":"Chinese Academy of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322696733"],"host_organization_lineage_names":["Chinese Academy of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo yufang shouyi xuebao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077067683","display_name":"Hualan Chen","orcid":"https://orcid.org/0000-0001-8910-898X","observed_orcids":["https://orcid.org/0000-0001-8910-898X"]},"institutions":[{"id":"https://openalex.org/I169572211","display_name":"Northeast Agricultural University","ror":"https://ror.org/0515nd386","country_code":"CN","type":"education","lineage":["https://openalex.org/I169572211"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Hualan Chen","raw_affiliation_strings":["College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China","institution_ids":["https://openalex.org/I169572211"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5077067683"],"corresponding_institution_ids":["https://openalex.org/I169572211"],"apc_list":null,"apc_paid":null,"fwci":1.4242,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.82680517,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/swine-influenza-virus","display_name":"swine influenza virus","score":0.9959999918937683},{"id":"https://openalex.org/keywords/h1n1-subtype","display_name":"H1N1 subtype","score":0.7390000224113464},{"id":"https://openalex.org/keywords/h1n2-subtype","display_name":"H1N2 subtype","score":1.0},{"id":"https://openalex.org/keywords/h3n2-subtype","display_name":"H3N2 subtype","score":1.0},{"id":"https://openalex.org/keywords/multiplex-rt-pcr","display_name":"multiplex RT-PCR","score":0.8970000147819519},{"id":"https://openalex.org/keywords/subtyping","display_name":"subtyping","score":0.6169999837875366},{"id":"https://openalex.org/keywords/hemagglutinin-gene","display_name":"hemagglutinin gene","score":0.9860000014305115},{"id":"https://openalex.org/keywords/neuraminidase-gene","display_name":"neuraminidase gene","score":0.9950000047683716},{"id":"https://openalex.org/keywords/rapid-detection","display_name":"rapid detection","score":0.6129999756813049},{"id":"https://openalex.org/keywords/pigs","display_name":"pigs","score":0.5830000042915344}],"concepts":[{"id":"https://openalex.org/C83852419","wikidata":"https://www.wikidata.org/wiki/Q2713292","display_name":"Subtyping","level":2,"score":0.8755605220794678},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8185107707977295},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.8101030588150024},{"id":"https://openalex.org/C129470801","wikidata":"https://www.wikidata.org/wiki/Q5370234","display_name":"Embryonated","level":3,"score":0.7963603734970093},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.6443452835083008},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.5991020202636719},{"id":"https://openalex.org/C2777546802","wikidata":"https://www.wikidata.org/wiki/Q834390","display_name":"Influenza A virus","level":3,"score":0.5355446338653564},{"id":"https://openalex.org/C79029880","wikidata":"https://www.wikidata.org/wiki/Q901755","display_name":"GenBank","level":3,"score":0.4843524694442749},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.4473086893558502},{"id":"https://openalex.org/C106358424","wikidata":"https://www.wikidata.org/wiki/Q1662346","display_name":"Infectivity","level":3,"score":0.43951311707496643},{"id":"https://openalex.org/C82988372","wikidata":"https://www.wikidata.org/wiki/Q5628866","display_name":"H5N1 genetic structure","level":5,"score":0.43519020080566406},{"id":"https://openalex.org/C32611913","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Titer","level":3,"score":0.4103408753871918},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.36973991990089417},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.36812371015548706},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13669365644454956},{"id":"https://openalex.org/C3008058167","wikidata":"https://www.wikidata.org/wiki/Q84263196","display_name":"Coronavirus disease 2019 (COVID-19)","level":4,"score":0.06255596876144409},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2392228588","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/article_en/cjfdtotal-zgxq200709012.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764717153","display_name":"Zhongguo yufang shouyi xuebao","issn_l":"1008-0589","issn":["1008-0589"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696733","host_organization_name":"Chinese Academy of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322696733"],"host_organization_lineage_names":["Chinese Academy of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo yufang shouyi xuebao","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1529081963","https://openalex.org/W2062829687","https://openalex.org/W2362739635","https://openalex.org/W2374574468","https://openalex.org/W114084559","https://openalex.org/W2032549585","https://openalex.org/W2141728269","https://openalex.org/W1966048083","https://openalex.org/W2000683540","https://openalex.org/W1979978212"],"abstract_inverted_index":{"Two":[0],"multiplex":[1,185],"RT-PCR":[2,50,186],"assays":[3,95,187],"specific":[4,98],"for":[5,165,193],"influenza":[6,29,48,65,111,146,202],"virus":[7,147],"H1":[8],"and":[9,11,24,31,41,55,60,84,87,99,149,155,168,196],"H3,N1":[10],"N2":[12],"subtypes":[13],"were":[14,16,96,151,162,172],"developed.Primers":[15],"designed":[17],"from":[18,43,105,124],"conserved":[19],"regions":[20],"of":[21,27,46,58,63,68,80,120,129,135,179,199],"the":[22,36,53,75,81,158,184],"HA":[23,54,83],"NA":[25,56,85],"genes":[26,57],"swine":[28,47,64,108,145,201],"H1N1,H1N2":[30,59,148],"H3N2":[32,61,150,169],"viruses":[33,79,112],"according":[34],"to":[35,126],"published":[37],"sequences":[38,42],"in":[39,153,175,181],"GenBank":[40],"China":[44],"isolates":[45],"virus.The":[49],"amplified":[51],"successfully":[52],"subtype":[62,86],"viruses.Sequence":[66],"analysis":[67],"PCR":[69,103],"products":[70,104],"by":[71],"BLAST":[72],"search":[73],"revealed":[74],"highest":[76],"identity":[77],"among":[78],"same":[82],"showed":[88,143],"complete":[89],"correlation":[90],"with":[91,177],"conventional":[92],"typing":[93],"methods.The":[94],"highly":[97],"did":[100],"not":[101],"amplify":[102],"3":[106,160],"common":[107],"pathogens":[109],"or":[110],"other":[113],"than":[114],"reference":[115,130],"strains;and":[116],"have":[117],"a":[118],"sensitivity":[119],"detecting":[121],"RNA":[122],"extracted":[123],"up":[125],"102":[127],"EID50":[128],"virus(with":[131],"original":[132],"infectivity":[133],"titer":[134],"107":[136],"EID50/0.1":[137],"mL).Test":[138],"against":[139],"40":[140],"positive":[141,164],"samples":[142,161],"that":[144],"identified":[152],"16,1":[154],"20":[156],"samples,respectively,while":[157],"remaining":[159],"detected":[163],"both":[166],"H1N1":[167],"viruses.These":[170],"results":[171],"100":[173],"%":[174],"agreement":[176],"those":[178],"virusisolated":[180],"embryonated":[182],"eggs.Therefore":[183],"could":[188],"be":[189],"an":[190],"effective":[191],"tool":[192],"rapid":[194],"detection":[195],"simultaneous":[197],"subtyping":[198],"clinical":[200],"virus.":[203]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}