{"id":"https://openalex.org/W2390126532","doi":null,"title":"Investigation of Difference of Alternative Splicing In Human Brain Based On PCR","display_name":"Investigation of Difference of Alternative Splicing In Human Brain Based On PCR","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2390126532","mag":"2390126532"},"language":"en","primary_location":{"id":"mag:2390126532","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-SWCX201206005.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764952023","display_name":"Xiandai shengwu yixue jinzhan","issn_l":"1673-6273","issn":["1673-6273"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727479","host_organization_name":"Heilongjiang Red Cross Society","host_organization_lineage":["https://openalex.org/P4361727479"],"host_organization_lineage_names":["Heilongjiang Red Cross Society"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xiandai shengwu yixue jinzhan","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014846775","display_name":"Ning\u2010Yi Shao","orcid":"https://orcid.org/0000-0003-4231-828X","observed_orcids":["https://orcid.org/0000-0003-4231-828X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shao Ning-yi","raw_affiliation_strings":["Philipp Khaitovich2,YANG Hong-yi1"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Philipp Khaitovich2,YANG Hong-yi1","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029377084","display_name":"Mpg Partner","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mpg Partner","raw_affiliation_strings":["320 Yueyang Road,200031,Shanghai,China)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"320 Yueyang Road,200031,Shanghai,China)","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.23140037,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":1.0},{"id":"https://openalex.org/keywords/brain-aging","display_name":"brain aging","score":0.8489999771118164},{"id":"https://openalex.org/keywords/prefrontal-cortex","display_name":"prefrontal cortex","score":0.9919999837875366},{"id":"https://openalex.org/keywords/splicing-sites","display_name":"splicing sites","score":0.7940000295639038},{"id":"https://openalex.org/keywords/transcriptome","display_name":"transcriptome","score":0.5950000286102295},{"id":"https://openalex.org/keywords/mrna","display_name":"mRNA","score":0.3050000071525574}],"concepts":[{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.8915240168571472},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.7940691709518433},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.6249618530273438},{"id":"https://openalex.org/C2781195155","wikidata":"https://www.wikidata.org/wiki/Q18680","display_name":"Prefrontal cortex","level":3,"score":0.603455662727356},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5890998840332031},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.5693647265434265},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.4999518394470215},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4900439977645874},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.4879605174064636},{"id":"https://openalex.org/C47124935","wikidata":"https://www.wikidata.org/wiki/Q468289","display_name":"Trans-splicing","level":5,"score":0.43324440717697144},{"id":"https://openalex.org/C107397762","wikidata":"https://www.wikidata.org/wiki/Q2542347","display_name":"RNA-Seq","level":5,"score":0.4263843595981598},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.41077476739883423},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36754870414733887},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3298869729042053},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.29742032289505005},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.13673937320709229},{"id":"https://openalex.org/C169900460","wikidata":"https://www.wikidata.org/wiki/Q2200417","display_name":"Cognition","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2390126532","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-SWCX201206005.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764952023","display_name":"Xiandai shengwu yixue jinzhan","issn_l":"1673-6273","issn":["1673-6273"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727479","host_organization_name":"Heilongjiang Red Cross Society","host_organization_lineage":["https://openalex.org/P4361727479"],"host_organization_lineage_names":["Heilongjiang Red Cross Society"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xiandai shengwu yixue jinzhan","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.7246000170707703,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4387017549","https://openalex.org/W2146646792","https://openalex.org/W7099816567","https://openalex.org/W6998504798","https://openalex.org/W4403450082","https://openalex.org/W4239549485","https://openalex.org/W4415924794","https://openalex.org/W7117702528","https://openalex.org/W2024190032","https://openalex.org/W7132832933"],"abstract_inverted_index":{"Objective:":[0],"To":[1],"investigate":[2],"the":[3,15,67],"aging":[4],"related":[5],"alternative":[6,70],"splicing":[7,12,65,71],"by":[8,27,51],"PCR.Methods:":[9],"Differences":[10],"in":[11,18,44],"sites":[13],"of":[14,21,33,69],"prefrontal":[16],"cortex(PFC)":[17],"brain":[19],"samples":[20,41],"2":[22],"different":[23,64],"ages":[24],"were":[25,35,42,57,60],"detected":[26],"transcriptome":[28],"RNA-Seq":[29],"data":[30],"analysis.Seven":[31],"pairs":[32],"primers":[34],"designed":[36],"separately.The":[37],"mRNA":[38],"extracted":[39],"from":[40],"mixed":[43],"equal":[45],"amount,and":[46],"then":[47],"cDNA":[48],"was":[49],"synthesized":[50],"reverse":[52],"transcription.Results:":[53],"The":[54],"target":[55],"sequences":[56],"amplified.Conclusions:":[58],"There":[59],"clear":[61],"bands":[62],"for":[63],"pattern,established":[66],"existence":[68],"differences":[72],"between":[73],"ages.":[74]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}