{"id":"https://openalex.org/W2386602005","doi":null,"title":"Effects of Three Vitrification Methods on the Microfilament Distribution and Lipid Droplets Changes of Porcine MII-stage Oocytes","display_name":"Effects of Three Vitrification Methods on the Microfilament Distribution and Lipid Droplets Changes of Porcine MII-stage Oocytes","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2386602005","mag":"2386602005"},"language":"en","primary_location":{"id":"mag:2386602005","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-XMSY201407011.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764778728","display_name":"Xumu shouyi xuebao","issn_l":"0366-6964","issn":["0366-6964"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4323999827","host_organization_name":"CHINA INT BOOK TRADING CORP","host_organization_lineage":["https://openalex.org/P4323999827"],"host_organization_lineage_names":["CHINA INT BOOK TRADING CORP"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xumu shouyi xuebao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5032503186","display_name":"WU Cai-fen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I148128674","display_name":"University of Shanghai for Science and Technology","ror":"https://ror.org/00ay9v204","country_code":"CN","type":"education","lineage":["https://openalex.org/I148128674"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"WU Cai-fen","raw_affiliation_strings":["Institute of Biothermal Science and Technology,University of Shanghai for Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Biothermal Science and Technology,University of Shanghai for Science and Technology","institution_ids":["https://openalex.org/I148128674"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5032503186"],"corresponding_institution_ids":["https://openalex.org/I148128674"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.34463625,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vitrification","display_name":"vitrification","score":0.9309999942779541},{"id":"https://openalex.org/keywords/porcine-oocytes","display_name":"porcine oocytes","score":0.953000009059906},{"id":"https://openalex.org/keywords/metaphase-ii-oocytes","display_name":"metaphase II oocytes","score":0.8880000114440918},{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.7490000128746033},{"id":"https://openalex.org/keywords/lipid-droplets","display_name":"lipid droplets","score":0.9919999837875366},{"id":"https://openalex.org/keywords/cooling-rate","display_name":"cooling rate","score":0.7960000038146973},{"id":"https://openalex.org/keywords/oocyte-cryopreservation","display_name":"oocyte cryopreservation","score":0.8320000171661377},{"id":"https://openalex.org/keywords/ultrastructure","display_name":"ultrastructure","score":0.5260000228881836},{"id":"https://openalex.org/keywords/oocyte-damage","display_name":"oocyte damage","score":0.5640000104904175}],"concepts":[{"id":"https://openalex.org/C25095133","wikidata":"https://www.wikidata.org/wiki/Q573950","display_name":"Lipid droplet","level":2,"score":0.7980278730392456},{"id":"https://openalex.org/C2778022349","wikidata":"https://www.wikidata.org/wiki/Q1072553","display_name":"Vitrification","level":2,"score":0.7597392797470093},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6103958487510681},{"id":"https://openalex.org/C87555872","wikidata":"https://www.wikidata.org/wiki/Q3141316","display_name":"Ultrastructure","level":2,"score":0.5783965587615967},{"id":"https://openalex.org/C2779587293","wikidata":"https://www.wikidata.org/wiki/Q7622360","display_name":"Straw","level":2,"score":0.5113598704338074},{"id":"https://openalex.org/C189040839","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Microfilament","level":4,"score":0.4479234218597412},{"id":"https://openalex.org/C146088050","wikidata":"https://www.wikidata.org/wiki/Q744818","display_name":"Transmission electron microscopy","level":2,"score":0.4223022162914276},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.38299453258514404},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.37882304191589355},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.3030090630054474},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2792930006980896},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.27355697751045227},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24008789658546448},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.20615404844284058},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.13464954495429993},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.11186426877975464},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.07291096448898315},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2386602005","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-XMSY201407011.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764778728","display_name":"Xumu shouyi xuebao","issn_l":"0366-6964","issn":["0366-6964"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4323999827","host_organization_name":"CHINA INT BOOK TRADING CORP","host_organization_lineage":["https://openalex.org/P4323999827"],"host_organization_lineage_names":["CHINA INT BOOK TRADING CORP"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xumu shouyi xuebao","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2349177796","https://openalex.org/W2754768316","https://openalex.org/W7036741326","https://openalex.org/W2117571835","https://openalex.org/W2069293047","https://openalex.org/W1979515566","https://openalex.org/W2090864110","https://openalex.org/W2341076931","https://openalex.org/W3136944012","https://openalex.org/W2007473878"],"abstract_inverted_index":{"The":[0],"purpose":[1],"of":[2,36,70,86,90,108,119,143,150],"this":[3],"study":[4],"was":[5,39,53,61],"to":[6],"determine":[7],"the":[8,40,47,66,126,141,147],"microfilament":[9],"distribution,lipid":[10],"droplets":[11,72,93,110,128],"and":[12,28,75,116,129],"their":[13,130],"ultrastructure":[14],"changes":[15],"that":[16,85,125],"resulted":[17],"from":[18],"vitrified":[19],"porcine":[20],"MII-stage":[21],"oocytes":[22,96,113],"with":[23,103],"3vitrification":[24],"techniques(Straw":[25],"method,CLV":[26],"method":[27,38,45],"OPS":[29,57,76],"method).The":[30],"results":[31,123],"showed":[32],"that:(1)The":[33],"cooling":[34,137],"speed":[35,138],"CLV":[37,74],"highest,and":[41],"got":[42,46],"42":[43],"639\u2103\u00b7min-1.(2)CLV":[44],"highest":[48],"normal":[49,67],"distribution":[50,68,131,151],"rate(47.06%),and":[51],"it":[52],"much":[54,82],"higher":[55,83],"than":[56,84],"method(38.04%)and":[58],"Straw":[59,87],"method(30.95%).(3)There":[60],"no":[62],"significant":[63],"differences":[64],"in":[65,94,111,152],"rate":[69],"lipid":[71,92,109,127,153],"between":[73],"methods":[77],"after":[78,100],"thaw,but":[79],"they":[80],"were":[81,97,114,121,134],"method(P0.05).(4)Decreased":[88],"content":[89],"large":[91],"thawed":[95,112],"found":[98],"out":[99],"ultrastructural":[101],"observation":[102],"transmission":[104],"electron":[105],"microscopy(TEM),and":[106],"most":[107],"homogeneous":[115],"very":[117],"few":[118],"them":[120],"heterogeneous.These":[122],"concluded":[124],"af-ter":[132],"vitrification":[133],"damaged":[135],"greatly.Improving":[136],"could":[139],"decrease":[140],"injury":[142],"microfilament,and":[144],"then":[145],"reduce":[146],"damage":[148],"degree":[149],"droplets.":[154]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}