{"id":"https://openalex.org/W2386333346","doi":null,"title":"The construction of rat IL-6 gene siRNA expression vector","display_name":"The construction of rat IL-6 gene siRNA expression vector","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W2386333346","mag":"2386333346"},"language":"en","primary_location":{"id":"mag:2386333346","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-XJYY200709004.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764643662","display_name":"Xinjiang Yike Daxue xuebao","issn_l":"1009-5551","issn":["1009-5551"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727539","host_organization_name":"Xinjiang Medical University","host_organization_lineage":["https://openalex.org/P4361727539"],"host_organization_lineage_names":["Xinjiang Medical University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xinjiang Yike Daxue xuebao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103140652","display_name":"Mei Long","orcid":"https://orcid.org/0009-0005-5955-1733","observed_orcids":["https://orcid.org/0009-0005-5955-1733"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Mei Long","raw_affiliation_strings":["Halmurat\u00b7wufuer, et al"],"raw_orcid":"https://orcid.org/0009-0005-5955-1733","affiliations":[{"raw_affiliation_string":"Halmurat\u00b7wufuer, et al","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5103140652"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.24860025,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11668","display_name":"interferon and immune responses","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/interleukin-6","display_name":"interleukin-6","score":0.8730000257492065},{"id":"https://openalex.org/keywords/sirna-expression-vector","display_name":"siRNA expression vector","score":0.7570000290870667},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.9390000104904175},{"id":"https://openalex.org/keywords/gene-knockdown","display_name":"gene knockdown","score":0.765999972820282}],"concepts":[{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.7982706427574158},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.6656646728515625},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6022477746009827},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5952935814857483},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.578408420085907},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.524231493473053},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5084606409072876},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.48060959577560425},{"id":"https://openalex.org/C92087593","wikidata":"https://www.wikidata.org/wiki/Q427389","display_name":"Vector (molecular biology)","level":4,"score":0.47803792357444763},{"id":"https://openalex.org/C81089528","wikidata":"https://www.wikidata.org/wiki/Q5134986","display_name":"clone (Java method)","level":3,"score":0.47580820322036743},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.4648522138595581},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.43963271379470825},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4160114526748657},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2608836889266968},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.15491583943367004}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2386333346","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-XJYY200709004.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764643662","display_name":"Xinjiang Yike Daxue xuebao","issn_l":"1009-5551","issn":["1009-5551"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727539","host_organization_name":"Xinjiang Medical University","host_organization_lineage":["https://openalex.org/P4361727539"],"host_organization_lineage_names":["Xinjiang Medical University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Xinjiang Yike Daxue xuebao","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2366334775","https://openalex.org/W2350999683","https://openalex.org/W2355087730","https://openalex.org/W2373775211","https://openalex.org/W2381325389","https://openalex.org/W2386116808","https://openalex.org/W1968070727","https://openalex.org/W2355688609","https://openalex.org/W2355126468","https://openalex.org/W2374330205"],"abstract_inverted_index":{"Objective:":[0],"To":[1],"construct":[2],"the":[3,14,28,55,78,98,121],"specific":[4,42],"small":[5],"interfereing":[6],"RNA":[7,21,130],"(siRNA)":[8],"expression":[9,66,101,113],"vector":[10,67,102,114],"that":[11,97],"can":[12],"block":[13],"rat":[15,34,110],"interleukin":[16],"6":[17],"(IL-6)":[18],"gene":[19,69,123],"by":[20,37,53,68,82,90,129],"interference":[22],"(RNAi)":[23],"technique.":[24,132],"Methods:":[25],"According":[26],"to":[27],"encoding":[29],"sequence":[30],"of":[31,33,41,109,119,124],"mRNA":[32],"IL-6":[35,99,111],"provided":[36],"Genebank,":[38],"two":[39],"pieces":[40],"hairpin":[43],"structure":[44],"short":[45],"chain":[46],"oligonucleotide":[47,60],"sequences":[48],"were":[49,62],"designed":[50],"and":[51,72,86,94],"synthesized":[52],"using":[54,83,91],"siRNA":[56,100,112],"software.":[57],"The":[58],"annealed":[59],"fragment":[61],"subcloned":[63],"into":[64,74],"pSilencerTM1.0-U6":[65],"clone":[70],"technique":[71],"transformed":[73],"DH5\u03b1":[75],"E.coli.":[76],"Finally":[77],"plasmid":[79],"was":[80,103],"evaluated":[81],"enzyme":[84,92],"cutting":[85,93],"sequencing.":[87],"Results:":[88],"Identification":[89],"sequencing":[95],"showed":[96],"constructed":[104],"successfully.":[105],"Conclusion:":[106],"Successful":[107],"construction":[108],"will":[115],"facilitate":[116],"further":[117],"studies":[118],"determine":[120],"onset":[122],"diseases":[125],"on":[126],"animal":[127],"model":[128],"interference(RNAi)":[131]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}