{"id":"https://openalex.org/W2379032003","doi":null,"title":"Establishment of a cDNA-AFLP System in Ammopiptanthus mongolicus and Primer Screening","display_name":"Establishment of a cDNA-AFLP System in Ammopiptanthus mongolicus and Primer Screening","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2379032003","mag":"2379032003"},"language":"en","primary_location":{"id":"mag:2379032003","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-AHNY200924046.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764869649","display_name":"Anhui nongye kexue","issn_l":"0517-6611","issn":["0517-6611"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696733","host_organization_name":"Chinese Academy of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322696733"],"host_organization_lineage_names":["Chinese Academy of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Anhui nongye kexue","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100462166","display_name":"Dandan Wang","orcid":"https://orcid.org/0000-0002-5681-7488","observed_orcids":["https://orcid.org/0000-0002-5681-7488"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Dandan Wang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5681-7488","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5100462166"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6118000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6118000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10014","display_name":"Plant Stress Responses and Tolerance","score":0.07590000331401825,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.0551999993622303,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ammopiptanthus-mongolicus","display_name":"Ammopiptanthus mongolicus","score":0.9959999918937683},{"id":"https://openalex.org/keywords/cdna-aflp","display_name":"cDNA-AFLP","score":0.9929999709129333},{"id":"https://openalex.org/keywords/stress-responsive-genes","display_name":"stress response genes","score":0.718999981880188},{"id":"https://openalex.org/keywords/plant-stress-tolerance","display_name":"plant stress tolerance","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C205713591","wikidata":"https://www.wikidata.org/wiki/Q4463029","display_name":"Trizol","level":5,"score":0.8062602877616882},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7780101299285889},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.6858308911323547},{"id":"https://openalex.org/C153523973","wikidata":"https://www.wikidata.org/wiki/Q292077","display_name":"Amplified fragment length polymorphism","level":4,"score":0.5763158798217773},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.538629412651062},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49728062748908997},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.363571435213089},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3261767029762268},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23563408851623535},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.22925537824630737},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05822679400444031},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.04564806818962097},{"id":"https://openalex.org/C81977670","wikidata":"https://www.wikidata.org/wiki/Q585259","display_name":"Genetic diversity","level":3,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2379032003","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-AHNY200924046.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764869649","display_name":"Anhui nongye kexue","issn_l":"0517-6611","issn":["0517-6611"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696733","host_organization_name":"Chinese Academy of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322696733"],"host_organization_lineage_names":["Chinese Academy of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Anhui nongye kexue","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2056200816","https://openalex.org/W2392513163","https://openalex.org/W2393280447","https://openalex.org/W3152228448","https://openalex.org/W2363813615","https://openalex.org/W2265823993","https://openalex.org/W2353104654","https://openalex.org/W2383834526","https://openalex.org/W2387526992","https://openalex.org/W2233924343"],"abstract_inverted_index":{"[Objective]":[0],"The":[1,68],"research":[2,69],"aimed":[3],"to":[4,25],"establish":[5],"the":[6,16,39,77],"cDNA-AFLP":[7,66],"system":[8],"in":[9,42,80],"Ammopiptanthus":[10,43,81],"mongolicus.[Method]":[11],"RNA":[12,27,41],"was":[13],"extracted":[14],"from":[15,57],"control":[17],"and":[18,22],"three":[19],"stress-treatment":[20],"materials.Trizol":[21],"CTAB":[23,31],"method":[24,32],"extract":[26],"were":[28,55,63],"compared.[Result]":[29],"Improved":[30],"could":[33,70],"obtain":[34],"a":[35],"higher":[36],"quality":[37],"of":[38],"total":[40],"mongolicus.Using":[44],"EcoR":[45],"\u2160":[46,48],"/Mse":[47],"digestion":[49],"system,35":[50],"selective":[51,59],"amplification":[52,60],"primer":[53,61],"pairs":[54],"identified":[56],"64":[58],"pairs,which":[62],"suitable":[64],"for":[65,74],"analysis.[Conclusion]":[67],"provide":[71],"important":[72],"evidence":[73],"further":[75],"studying":[76],"stress-response":[78],"genes":[79],"mongolicus.":[82]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}