{"id":"https://openalex.org/W2376786711","doi":null,"title":"Production and characterisation of putative early effector proteins in Chlamydia trachomatis infection","display_name":"Production and characterisation of putative early effector proteins in Chlamydia trachomatis infection","publication_year":2015,"publication_date":"2015-12-01","ids":{"openalex":"https://openalex.org/W2376786711","mag":"2376786711"},"language":"en","primary_location":{"id":"pmh:oai:etheses.whiterose.ac.uk:12544","doi":null,"is_oa":false,"landing_page_url":"http://etheses.whiterose.ac.uk/12544/1/JCMRes_thesisPDF_final_Dec15.pdf","pdf_url":"http://etheses.whiterose.ac.uk/12544/1/JCMRes_thesisPDF_final_Dec15.pdf","source":{"id":"https://openalex.org/S4306401585","display_name":"White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2800616092","host_organization_name":"White Rose University Consortium","host_organization_lineage":["https://openalex.org/I2800616092"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063014998","display_name":"Jamie Coldwell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jamie Coldwell","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5063014998"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.03420000150799751,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.022299999371170998,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chlamydia-trachomatis","display_name":"Chlamydia trachomatis","score":1.0},{"id":"https://openalex.org/keywords/bacterial-engulfment","display_name":"bacterial engulfment","score":0.7149999737739563},{"id":"https://openalex.org/keywords/trachoma","display_name":"trachoma","score":0.7289999723434448},{"id":"https://openalex.org/keywords/vaccine-targets","display_name":"vaccine targets","score":0.5450000166893005},{"id":"https://openalex.org/keywords/outer-membrane-proteins","display_name":"outer membrane proteins","score":0.7099999785423279},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7839999794960022},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.7429999709129333},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.7620000243186951},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.5149999856948853}],"concepts":[{"id":"https://openalex.org/C2777391075","wikidata":"https://www.wikidata.org/wiki/Q131065","display_name":"Chlamydia trachomatis","level":2,"score":0.8526411056518555},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6297323703765869},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5938096642494202},{"id":"https://openalex.org/C2777034029","wikidata":"https://www.wikidata.org/wiki/Q193216","display_name":"Trachoma","level":2,"score":0.569581925868988},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.49084216356277466},{"id":"https://openalex.org/C2776334974","wikidata":"https://www.wikidata.org/wiki/Q694552","display_name":"Lymphogranuloma venereum","level":3,"score":0.4873872399330139},{"id":"https://openalex.org/C2780122209","wikidata":"https://www.wikidata.org/wiki/Q153356","display_name":"Chlamydia","level":2,"score":0.458177775144577},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.44828522205352783},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.42825886607170105},{"id":"https://openalex.org/C146587185","wikidata":"https://www.wikidata.org/wiki/Q258248","display_name":"Bacterial outer membrane","level":4,"score":0.4269920587539673},{"id":"https://openalex.org/C179470777","wikidata":"https://www.wikidata.org/wiki/Q356661","display_name":"Bacterial adhesin","level":4,"score":0.4193785786628723},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.37034571170806885},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3615703582763672},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3303629755973816},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.26387423276901245},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.186568021774292},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:etheses.whiterose.ac.uk:12544","doi":null,"is_oa":false,"landing_page_url":"http://etheses.whiterose.ac.uk/12544/1/JCMRes_thesisPDF_final_Dec15.pdf","pdf_url":"http://etheses.whiterose.ac.uk/12544/1/JCMRes_thesisPDF_final_Dec15.pdf","source":{"id":"https://openalex.org/S4306401585","display_name":"White Rose eTheses Online (University of Leeds, The University of Sheffield, University of York)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2800616092","host_organization_name":"White Rose University Consortium","host_organization_lineage":["https://openalex.org/I2800616092"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2376786711","doi":null,"is_oa":false,"landing_page_url":"https://etheses.whiterose.ac.uk/12544/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1716361599","https://openalex.org/W1831717060","https://openalex.org/W1971389070","https://openalex.org/W1984028045","https://openalex.org/W1985338680","https://openalex.org/W1985947029","https://openalex.org/W2000570171","https://openalex.org/W2004023754","https://openalex.org/W2004920406","https://openalex.org/W2055109794","https://openalex.org/W2079805712","https://openalex.org/W2085134477","https://openalex.org/W2104099299","https://openalex.org/W2105786643","https://openalex.org/W2108344016","https://openalex.org/W2125065061","https://openalex.org/W2127363917","https://openalex.org/W2137273150","https://openalex.org/W2146888113","https://openalex.org/W2153833219","https://openalex.org/W2166597095","https://openalex.org/W2299489763"],"related_works":["https://openalex.org/W2149598491","https://openalex.org/W92389345","https://openalex.org/W2585870096","https://openalex.org/W2551766135","https://openalex.org/W2799558776","https://openalex.org/W808418293","https://openalex.org/W2364022489","https://openalex.org/W2605377860","https://openalex.org/W2104099299","https://openalex.org/W3031796566"],"abstract_inverted_index":{"Chlamydia":[0],"trachomatis":[1,50,138],"is":[2,39,47,117,130,140],"commonly":[3],"known":[4,42,73],"as":[5,124,128,249],"the":[6,14,21,44,53,57,78,82,111,114,133,152,214,234,252,267,270],"most":[7],"common":[8],"bacterial":[9],"sexually":[10],"transmitted":[11],"infection":[12,83],"in":[13,24,33,77,102],"world,":[15],"having":[16],"affected":[17],"approximately":[18],"3.1%":[19],"of":[20,31,60,81,110,136,177,181,242,255],"global":[22],"population":[23,46],"2010,":[25],"and":[26,35,69,87,90,99,139,164,187,191,205,228,239,245,274],"being":[27],"a":[28,118,125,167,200,206],"major":[29],"cause":[30,59],"infertility":[32],"men":[34],"women.":[36],"However,":[37],"what":[38],"less":[40],"well":[41],"among":[43,144],"general":[45],"that":[48],"C.":[49,137],"also":[51],"causes":[52],"blindness":[54,62],"disease":[55],"trachoma,":[56],"leading":[58],"preventable":[61],"globally.":[63],"This":[64],"project":[65,148,264],"aimed":[66],"to":[67,74,94,107,172],"produce":[68],"investigate":[70],"three":[71],"proteins":[72],"be":[75],"involved":[76],"early":[79],"stages":[80],"process:":[84],"CT694,":[85],"CT166":[86,91,246],"PmpD.":[88],"CT694":[89],"are":[92],"believed":[93],"affect":[95],"actin":[96],"fibre":[97],"formation":[98],"breakdown,":[100],"acting":[101],"concert":[103],"with":[104],"one":[105],"another":[106],"facilitate":[108],"engulfment":[109],"bacterium":[112],"by":[113,224],"host.":[115],"PmpD":[116,216,243],"protein":[119,182],"which":[120,169],"draws":[121],"significant":[122],"interest":[123],"vaccination":[126],"target,":[127],"it":[129],"present":[131],"on":[132,151,237],"outer":[134],"membrane":[135],"conserved":[141],"very":[142],"highly":[143],"serovars.":[145],"\\n":[146],"\\n\\tThis":[147],"focussed":[149],"mainly":[150,189],"breakdown":[153],"product":[154],"\u0394CT694.":[155],"Its":[156],"gene":[157,240],"was":[158,170,218,247,260],"amplified":[159],"from":[160],"Chlamydial":[161],"genomic":[162],"DNA":[163],"inserted":[165],"into":[166],"plasmid,":[168],"used":[171],"transform":[173],"an":[174],"expression":[175],"strain":[176],"Escherichia":[178],"coli.":[179],"Batches":[180],"were":[183,198,231],"produced":[184,219],"for":[185,221,272],"characterisation":[186,229],"investigation,":[188],"commercial":[190],"customised":[192],"crystallisation":[193],"screens.":[194],"Although":[195,251],"no":[196],"crystals":[197,259],"achieved,":[199],"promising":[201],"microcrystalline":[202],"precipitate":[203],"formed,":[204],"\u201cbest":[207],"condition\u201d":[208],"has":[209,265],"been":[210],"recorded.":[211],"In":[212],"addition,":[213],"fragment":[215],"F2":[217],"regularly":[220],"vaccine":[222],"studies":[223],"inclusion":[225],"body":[226],"preparation,":[227],"experiments":[230],"performed":[232,248],"upon":[233],"protein.":[235],"Work":[236],"PCR":[238],"cloning":[241],"fragments":[244],"well.":[250],"primary":[253],"goal":[254],"producing":[256],"X-ray":[257],"diffractable":[258],"not":[261],"met,":[262],"this":[263],"laid":[266],"foundations":[268],"within":[269],"group":[271],"development":[273],"future":[275],"work.":[276]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}