{"id":"https://openalex.org/W2376677173","doi":null,"title":"[Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells].","display_name":"[Expression of a secreted fusion protein consisting of anti-HER2 antibody and reversed caspase-3 and its suppression on growth of SKOV3 cells].","publication_year":2003,"publication_date":"2003-03-01","ids":{"openalex":"https://openalex.org/W2376677173","mag":"2376677173","pmid":"https://pubmed.ncbi.nlm.nih.gov/15151756"},"language":"zh","primary_location":{"id":"pmid:15151756","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15151756","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100705833","display_name":"Lihong Zhang","orcid":"https://orcid.org/0000-0003-0092-7984","observed_orcids":["https://orcid.org/0000-0003-0092-7984"]},"institutions":[{"id":"https://openalex.org/I9916479","display_name":"Air Force Medical University","ror":"https://ror.org/00ms48f15","country_code":"CN","type":"education","lineage":["https://openalex.org/I9916479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li-Hong Zhang","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, China","institution_ids":["https://openalex.org/I9916479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031464945","display_name":"Lintao Jia","orcid":"https://orcid.org/0000-0001-6329-0453","observed_orcids":["https://orcid.org/0000-0001-6329-0453"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lin-Tao Jia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047388991","display_name":"Wei Bao","orcid":"https://orcid.org/0000-0002-7301-5786","observed_orcids":["https://orcid.org/0000-0002-7301-5786"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wei Bao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100946008","display_name":"Cui-Juan Yu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cui-Juan Yu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103833935","display_name":"Jing Zhao","orcid":"https://orcid.org/0009-0005-2126-7614","observed_orcids":["https://orcid.org/0009-0005-2126-7614"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jing Zhao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020723004","display_name":"Ming Jin","orcid":"https://orcid.org/0000-0003-1962-2777","observed_orcids":["https://orcid.org/0000-0003-1962-2777"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ming Jin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004686722","display_name":"Chengji Wang","orcid":"https://orcid.org/0000-0003-3331-9117","observed_orcids":["https://orcid.org/0000-0003-3331-9117"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cheng-Ji Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5100741624","display_name":"Angang Yang","orcid":"https://orcid.org/0000-0002-8854-4672","observed_orcids":["https://orcid.org/0000-0002-8854-4672"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"An-Gang Yang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.23685896,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"19","issue":"2","first_page":"160","last_page":"2"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.7314000129699707,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.7314000129699707,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.18529999256134033,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.0421999990940094,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/her2-antibody","display_name":"HER2 antibody","score":0.7739999890327454},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.8809999823570251},{"id":"https://openalex.org/keywords/fusion-protein","display_name":"fusion protein","score":0.7229999899864197},{"id":"https://openalex.org/keywords/secreted-proteins","display_name":"secreted proteins","score":0.8349999785423279},{"id":"https://openalex.org/keywords/skov-3-cells","display_name":"SKOV3 cells","score":0.9110000133514404},{"id":"https://openalex.org/keywords/ovarian-cancer","display_name":"ovarian cancer","score":0.8320000171661377},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.4000000059604645},{"id":"https://openalex.org/keywords/targeted-cell-killing","display_name":"targeted cell killing","score":0.5690000057220459},{"id":"https://openalex.org/keywords/jurkat-cells","display_name":"Jurkat cells","score":0.7120000123977661},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.6959999799728394}],"concepts":[{"id":"https://openalex.org/C179464577","wikidata":"https://www.wikidata.org/wiki/Q1632589","display_name":"Jurkat cells","level":4,"score":0.9790114164352417},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.7895382642745972},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7886922359466553},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6552321910858154},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6115312576293945},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5424521565437317},{"id":"https://openalex.org/C111829193","wikidata":"https://www.wikidata.org/wiki/Q1108970","display_name":"Fusion gene","level":3,"score":0.47760578989982605},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.4675511419773102},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4651051461696625},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.423397958278656},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.41380468010902405},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4075544476509094},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3836742341518402},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.3198190927505493},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.29466712474823},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.18547606468200684},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11425954103469849},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09444141387939453},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053148","descriptor_name":"Caspase 3","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":1,"locations":[{"id":"pmid:15151756","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15151756","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2370006513","https://openalex.org/W2122377293","https://openalex.org/W2391159067","https://openalex.org/W2358838104","https://openalex.org/W882423228","https://openalex.org/W2265957375","https://openalex.org/W2999004448","https://openalex.org/W7043821824","https://openalex.org/W1993567918","https://openalex.org/W2036437239"],"abstract_inverted_index":{"AIM:":[0],"To":[1],"investigate":[2],"the":[3,16,44,59,80,84,93,107],"killing":[4],"effect":[5],"on":[6,62],"SKOV3":[7,65,97,113],"cells":[8,51,66,98,114],"by":[9,54,69],"fusion":[10,60,85,103],"protein":[11,61,86,104],"HER2-specific":[12],"antibody-reversed":[13],"caspase-3":[14,23],"in":[15,45,87,106],"secreted":[17,41,81,108],"form.":[18],"METHODS:":[19],"The":[20,40,56,102],"reversed":[21],"human":[22],"gene":[24],"was":[25,52,67,79],"subcloned":[26],"into":[27],"pCMV-e23scFv-PEII-PEIII":[28],"to":[29,99],"construct":[30],"recombinant":[31],"eukaryotic":[32],"expression":[33,42,82],"vector":[34],"pCMV-e23scFv-PEII-revcasp-3":[35],"and":[36,115],"transfect":[37],"Jurkat":[38,50,75,88],"cells.":[39,76,89],"of":[43,48,58,64,73,83,95],"culture":[46],"supernatant":[47,72],"transfected":[49,74],"detected":[53],"ELISA.":[55],"suppression":[57],"growth":[63],"examined":[68],"co-culture":[70],"with":[71],"RESULTS:":[77],"There":[78],"Expressed":[90],"product":[91],"had":[92],"activity":[94],"inducing":[96],"death.":[100],"CONCLUSION:":[101],"expressed":[105],"form":[109],"can":[110],"targeted":[111],"towards":[112],"kill":[116],"them.":[117]},"counts_by_year":[],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}