{"id":"https://openalex.org/W2376615012","doi":null,"title":"Cloning, Expression and Antibody Production of the Disintegrin Domain of Human Fertilin beta.","display_name":"Cloning, Expression and Antibody Production of the Disintegrin Domain of Human Fertilin beta.","publication_year":2001,"publication_date":"2001-01-01","ids":{"openalex":"https://openalex.org/W2376615012","mag":"2376615012","pmid":"https://pubmed.ncbi.nlm.nih.gov/12050790"},"language":"en","primary_location":{"id":"pmid:12050790","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12050790","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Bei-Bei Ding","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210127634","display_name":"Shanghai Institute of Planned Parenthood Research","ror":"https://ror.org/03awxj939","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210127634"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bei-Bei Ding","raw_affiliation_strings":["National Laboratory of Coutraceptives and Device Research, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China. shenwang@sippr.stc.sh.cn","National Laboratory of Coutraceptives and Device Research, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Laboratory of Coutraceptives and Device Research, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China. shenwang@sippr.stc.sh.cn","institution_ids":["https://openalex.org/I4210127634"]},{"raw_affiliation_string":"National Laboratory of Coutraceptives and Device Research, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China","institution_ids":["https://openalex.org/I4210127634"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Wei-Ying Shen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wei-Ying Shen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110609321","display_name":"Jiang Chu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Chu Jiang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Jian Wang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jian Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111830571","display_name":"Ersheng Gao","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Er-Sheng Gao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5103697042","display_name":"Qing-Xiang Shen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Qing-Xiang Shen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.27286245,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"33","issue":"3","first_page":"303","last_page":"308"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8187999725341797,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.8187999725341797,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.0333000011742115,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.02239999920129776,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fertilin-beta","display_name":"fertilin beta","score":0.9599999785423279},{"id":"https://openalex.org/keywords/disintegrin-domain","display_name":"disintegrin domain","score":0.9850000143051147},{"id":"https://openalex.org/keywords/cdna-cloning","display_name":"cDNA cloning","score":0.8730000257492065},{"id":"https://openalex.org/keywords/gst-fusion-protein","display_name":"GST fusion protein","score":0.656000018119812},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9139999747276306},{"id":"https://openalex.org/keywords/polyclonal-antibody-production","display_name":"polyclonal antibody production","score":0.9459999799728394},{"id":"https://openalex.org/keywords/testis","display_name":"testis","score":0.5419999957084656}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.8245896100997925},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.798949658870697},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.7576357126235962},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.6971321105957031},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5635651350021362},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5597356557846069},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5457605123519897},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5304117798805237},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.49071204662323},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.4734591841697693},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.4516167640686035},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4494195580482483},{"id":"https://openalex.org/C2778259572","wikidata":"https://www.wikidata.org/wiki/Q407908","display_name":"Disintegrin","level":4,"score":0.4319014549255371},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3522246479988098},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.33266812562942505},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31159359216690063},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.13380464911460876},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.05848705768585205},{"id":"https://openalex.org/C55728118","wikidata":"https://www.wikidata.org/wiki/Q425402","display_name":"Metalloproteinase","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmid:12050790","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12050790","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica","raw_type":"Journal Article"},{"id":"mag:2376615012","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12050790","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2360426166","https://openalex.org/W2350139232","https://openalex.org/W1965994848","https://openalex.org/W2214551808","https://openalex.org/W2374386099","https://openalex.org/W20227018","https://openalex.org/W234782229","https://openalex.org/W2354307296","https://openalex.org/W2392533538","https://openalex.org/W2348363764"],"abstract_inverted_index":{"A":[0],"cDNA":[1,20,47],"for":[2],"the":[3,31,75,86,127,138,175],"disintegrin":[4],"domain":[5],"(hf279)":[6],"was":[7,28,42,57,72,110,146],"isolated":[8,147],"by":[9,44,74,112,123,148],"PCR":[10],"from":[11,137],"human":[12],"testis":[13],"cDNAs.":[14],"DNA":[15],"sequencing":[16],"indicated":[17],"that":[18,85],"hf279":[19,46],"encoded":[21],"93":[22],"amino":[23],"acid":[24],"residues,":[25],"and":[26,63,98,121,153,156],"it":[27],"identical":[29],"with":[30,80,142],"reported":[32],"sequence":[33],"of":[34,67,95,103,140,177],"fertilin":[35],"beta.":[36],"An":[37],"expression":[38,55,76],"plasmid,":[39],"pGEX":[40],"hf279,":[41],"constructed":[43],"inserting":[45],"into":[48,59],"plasmid":[49,56],"pGEX-4T-2":[50],"containing":[51],"gst":[52],"gene.":[53],"The":[54,107],"introduced":[58],"E.coli":[60],"BL21(DE3)":[61],"cells":[62],"a":[64],"substantial":[65],"amount":[66],"soluble":[68,105],"fused":[69],"protein":[70,89,109],"GST-HF93":[71,87],"obtained":[73,136],"strain":[77],"HF93/BL21":[78],"induced":[79],"IPTG.":[81],"SDS-PAGE":[82],"analysis":[83,159],"revealed":[84],"fusion":[88,108],"had":[90],"an":[91],"apparent":[92],"molecular":[93],"weight":[94],"38":[96],"kD":[97],"accumulated":[99],"up":[100],"to":[101,125,163],"50%":[102],"bacterial":[104],"proteins.":[106],"purified":[111,128,143],"glutathione":[113],"S-transferase":[114],"(GST)":[115],"Sepharose":[116,150],"4B":[117,151],"column":[118,152],"(purity":[119,131],"90%)":[120],"digested":[122],"thrombin":[124],"obtain":[126],"HF93":[129,144],"peptide":[130],"80%).":[132],"Polyclonal":[133],"antibodies":[134],"were":[135],"serum":[139],"miceimmunized":[141],"which":[145],"GST":[149],"SDS-PAGE.":[154],"ELISA":[155],"Western":[157],"blot":[158],"showed":[160],"its":[161],"specificity":[162],"HF93.":[164,178],"Therefore":[165],"this":[166],"antibody":[167],"can":[168],"be":[169],"used":[170],"in":[171],"further":[172],"studies":[173],"on":[174],"function":[176]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}