{"id":"https://openalex.org/W2370189545","doi":null,"title":"Impact of xylose and mannose on central metabolism of yeast Saccharomyces cerevisiae","display_name":"Impact of xylose and mannose on central metabolism of yeast Saccharomyces cerevisiae","publication_year":2005,"publication_date":"2005-11-18","ids":{"openalex":"https://openalex.org/W2370189545","mag":"2370189545"},"language":"en","primary_location":{"id":"pmh:oai:aaltodoc.aalto.fi:123456789/2626","doi":null,"is_oa":true,"landing_page_url":"https://aaltodoc.aalto.fi/handle/123456789/2626","pdf_url":"https://aaltodoc.aalto.fi/bitstreams/f7b94f8f-437e-4126-8f14-22ad8aec6922/download","source":{"id":"https://openalex.org/S4306401662","display_name":"Aaltodoc (Aalto University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I9927081","host_organization_name":"Aalto University","host_organization_lineage":["https://openalex.org/I9927081"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"G5 Artikkeliv\u00e4it\u00f6skirja"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://aaltodoc.aalto.fi/bitstreams/f7b94f8f-437e-4126-8f14-22ad8aec6922/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066581248","display_name":"Juha\u2010Pekka Pitk\u00e4nen","orcid":"https://orcid.org/0000-0002-4006-0302","observed_orcids":["https://orcid.org/0000-0002-4006-0302"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Juha\u2010Pekka Pitk\u00e4nen","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-4006-0302","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5066581248"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9106000065803528,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9106000065803528,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.07169999927282333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.013399999588727951,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/xylose-utilization","display_name":"xylose utilization","score":0.8539999723434448},{"id":"https://openalex.org/keywords/d-mannose","display_name":"D-mannose","score":0.8560000061988831},{"id":"https://openalex.org/keywords/phosphomannose-isomerase","display_name":"phosphomannose isomerase","score":0.8790000081062317},{"id":"https://openalex.org/keywords/xylulose","display_name":"xylulose","score":0.6330000162124634},{"id":"https://openalex.org/keywords/oxidative-pentose-phosphate-pathway","display_name":"oxidative pentose phosphate pathway","score":0.8899999856948853},{"id":"https://openalex.org/keywords/tricarboxylic-acid-cycle","display_name":"TCA cycle","score":0.8050000071525574},{"id":"https://openalex.org/keywords/oxidative-phosphorylation","display_name":"oxidative phosphorylation","score":0.8840000033378601},{"id":"https://openalex.org/keywords/microaerobic-conditions","display_name":"microaerobic conditions","score":0.703000009059906},{"id":"https://openalex.org/keywords/xylose-absorption","display_name":"xylose uptake","score":0.7099999785423279},{"id":"https://openalex.org/keywords/central-carbon-metabolism","display_name":"central carbon metabolism","score":0.3630000054836273}],"concepts":[{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.7572604417800903},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.6569014191627502},{"id":"https://openalex.org/C2780294150","wikidata":"https://www.wikidata.org/wiki/Q66589603","display_name":"Xylose","level":3,"score":0.6096832752227783},{"id":"https://openalex.org/C2775887612","wikidata":"https://www.wikidata.org/wiki/Q10809118","display_name":"Mannose","level":2,"score":0.46549758315086365},{"id":"https://openalex.org/C2778447961","wikidata":"https://www.wikidata.org/wiki/Q1146293","display_name":"Saccharomyces","level":4,"score":0.42074090242385864},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.42011767625808716},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37351155281066895},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37288692593574524},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.1871209442615509}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:aaltodoc.aalto.fi:123456789/2626","doi":null,"is_oa":true,"landing_page_url":"https://aaltodoc.aalto.fi/handle/123456789/2626","pdf_url":"https://aaltodoc.aalto.fi/bitstreams/f7b94f8f-437e-4126-8f14-22ad8aec6922/download","source":{"id":"https://openalex.org/S4306401662","display_name":"Aaltodoc (Aalto University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I9927081","host_organization_name":"Aalto University","host_organization_lineage":["https://openalex.org/I9927081"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"G5 Artikkeliv\u00e4it\u00f6skirja"},{"id":"mag:2370189545","doi":null,"is_oa":false,"landing_page_url":"http://lib.tkk.fi/Diss/2005/isbn9512278944/isbn9512278944.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:aaltodoc.aalto.fi:123456789/2626","doi":null,"is_oa":true,"landing_page_url":"https://aaltodoc.aalto.fi/handle/123456789/2626","pdf_url":"https://aaltodoc.aalto.fi/bitstreams/f7b94f8f-437e-4126-8f14-22ad8aec6922/download","source":{"id":"https://openalex.org/S4306401662","display_name":"Aaltodoc (Aalto University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I9927081","host_organization_name":"Aalto University","host_organization_lineage":["https://openalex.org/I9927081"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"G5 Artikkeliv\u00e4it\u00f6skirja"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310086","display_name":"Helsingin Yliopisto","ror":"https://ror.org/040af2s02"},{"id":"https://openalex.org/F4320321855","display_name":"Tekes","ror":"https://ror.org/02ag8cq23"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2370189545.pdf","grobid_xml":"https://content.openalex.org/works/W2370189545.grobid-xml"},"referenced_works_count":206,"referenced_works":["https://openalex.org/W55455380","https://openalex.org/W182446545","https://openalex.org/W659488186","https://openalex.org/W1479334442","https://openalex.org/W1480463281","https://openalex.org/W1491459594","https://openalex.org/W1510585142","https://openalex.org/W1532986430","https://openalex.org/W1561767438","https://openalex.org/W1566830427","https://openalex.org/W1573427298","https://openalex.org/W1574470162","https://openalex.org/W1576919957","https://openalex.org/W1584704387","https://openalex.org/W1589316519","https://openalex.org/W1591600424","https://openalex.org/W1604959358","https://openalex.org/W1606153755","https://openalex.org/W1751931305","https://openalex.org/W1753761684","https://openalex.org/W1754822275","https://openalex.org/W1804208390","https://openalex.org/W1804584144","https://openalex.org/W1838774522","https://openalex.org/W1850754372","https://openalex.org/W1861998278","https://openalex.org/W1895267024","https://openalex.org/W1896630930","https://openalex.org/W1905945789","https://openalex.org/W1908662736","https://openalex.org/W1919855132","https://openalex.org/W1943333416","https://openalex.org/W1962003619","https://openalex.org/W1962823952","https://openalex.org/W1965399445","https://openalex.org/W1965997293","https://openalex.org/W1966645006","https://openalex.org/W1970122244","https://openalex.org/W1970783685","https://openalex.org/W1971411221","https://openalex.org/W1971548414","https://openalex.org/W1971761257","https://openalex.org/W1972140184","https://openalex.org/W1972758229","https://openalex.org/W1976245786","https://openalex.org/W1977576010","https://openalex.org/W1979630986","https://openalex.org/W1980393312","https://openalex.org/W1985341576","https://openalex.org/W1987551969","https://openalex.org/W1987939469","https://openalex.org/W1988120944","https://openalex.org/W1988608160","https://openalex.org/W1990520393","https://openalex.org/W1994855175","https://openalex.org/W1995494753","https://openalex.org/W1996119276","https://openalex.org/W1996845151","https://openalex.org/W1996974151","https://openalex.org/W1997493743","https://openalex.org/W2000047924","https://openalex.org/W2000870754","https://openalex.org/W2003155571","https://openalex.org/W2003294189","https://openalex.org/W2006477248","https://openalex.org/W2006704946","https://openalex.org/W2007548126","https://openalex.org/W2008141632","https://openalex.org/W2009200103","https://openalex.org/W2011559317","https://openalex.org/W2014258732","https://openalex.org/W2017361626","https://openalex.org/W2021234742","https://openalex.org/W2025925813","https://openalex.org/W2026054660","https://openalex.org/W2026481834","https://openalex.org/W2026909022","https://openalex.org/W2026923867","https://openalex.org/W2031847485","https://openalex.org/W2033570693","https://openalex.org/W2033591225","https://openalex.org/W2034645234","https://openalex.org/W2034862625","https://openalex.org/W2034875354","https://openalex.org/W2037036397","https://openalex.org/W2040675389","https://openalex.org/W2041946854","https://openalex.org/W2042082999","https://openalex.org/W2042473322","https://openalex.org/W2044929893","https://openalex.org/W2047979988","https://openalex.org/W2049032385","https://openalex.org/W2049624014","https://openalex.org/W2050858598","https://openalex.org/W2051451012","https://openalex.org/W2052372509","https://openalex.org/W2053126761","https://openalex.org/W2058845513","https://openalex.org/W2059258473","https://openalex.org/W2061459002","https://openalex.org/W2062617694","https://openalex.org/W2063136211","https://openalex.org/W2064249717","https://openalex.org/W2065163714","https://openalex.org/W2065258625","https://openalex.org/W2065949669","https://openalex.org/W2067443521","https://openalex.org/W2067734078","https://openalex.org/W2067958895","https://openalex.org/W2069905430","https://openalex.org/W2069953403","https://openalex.org/W2070955414","https://openalex.org/W2071070866","https://openalex.org/W2072106875","https://openalex.org/W2073253360","https://openalex.org/W2073506826","https://openalex.org/W2081827055","https://openalex.org/W2083669318","https://openalex.org/W2084619201","https://openalex.org/W2085771741","https://openalex.org/W2087096430","https://openalex.org/W2088127501","https://openalex.org/W2088933296","https://openalex.org/W2090533882","https://openalex.org/W2091137324","https://openalex.org/W2091778526","https://openalex.org/W2092522779","https://openalex.org/W2094613397","https://openalex.org/W2095975432","https://openalex.org/W2096202610","https://openalex.org/W2098601724","https://openalex.org/W2100371004","https://openalex.org/W2101397045","https://openalex.org/W2101442503","https://openalex.org/W2103453943","https://openalex.org/W2106362845","https://openalex.org/W2107849669","https://openalex.org/W2109370036","https://openalex.org/W2109757903","https://openalex.org/W2110443726","https://openalex.org/W2115253315","https://openalex.org/W2115470699","https://openalex.org/W2118439704","https://openalex.org/W2118956583","https://openalex.org/W2119645484","https://openalex.org/W2120978915","https://openalex.org/W2121911008","https://openalex.org/W2123280311","https://openalex.org/W2125620567","https://openalex.org/W2125709690","https://openalex.org/W2126548942","https://openalex.org/W2126602684","https://openalex.org/W2127754721","https://openalex.org/W2127862649","https://openalex.org/W2130316063","https://openalex.org/W2130325881","https://openalex.org/W2132454572","https://openalex.org/W2133403068","https://openalex.org/W2134224359","https://openalex.org/W2135998024","https://openalex.org/W2136947295","https://openalex.org/W2137683543","https://openalex.org/W2139893351","https://openalex.org/W2140670503","https://openalex.org/W2141034501","https://openalex.org/W2141322438","https://openalex.org/W2141413113","https://openalex.org/W2141652419","https://openalex.org/W2144631797","https://openalex.org/W2146546288","https://openalex.org/W2147454875","https://openalex.org/W2149470189","https://openalex.org/W2149842777","https://openalex.org/W2150750144","https://openalex.org/W2150775797","https://openalex.org/W2150904401","https://openalex.org/W2150910409","https://openalex.org/W2151785115","https://openalex.org/W2153065593","https://openalex.org/W2153223092","https://openalex.org/W2153558341","https://openalex.org/W2153560677","https://openalex.org/W2154292068","https://openalex.org/W2154666531","https://openalex.org/W2156027080","https://openalex.org/W2156692212","https://openalex.org/W2158749368","https://openalex.org/W2159100436","https://openalex.org/W2159482845","https://openalex.org/W2160443407","https://openalex.org/W2160998060","https://openalex.org/W2162891889","https://openalex.org/W2163531785","https://openalex.org/W2164963113","https://openalex.org/W2165130891","https://openalex.org/W2165762336","https://openalex.org/W2166690888","https://openalex.org/W2167609254","https://openalex.org/W2168636232","https://openalex.org/W2169104686","https://openalex.org/W2170778160","https://openalex.org/W2183948172","https://openalex.org/W2197217968","https://openalex.org/W4211039941","https://openalex.org/W4230501579","https://openalex.org/W7073557482"],"related_works":["https://openalex.org/W7096081125","https://openalex.org/W2097790673","https://openalex.org/W659177481","https://openalex.org/W1480585901","https://openalex.org/W2100262190","https://openalex.org/W2146662507","https://openalex.org/W818208480","https://openalex.org/W2153504900","https://openalex.org/W816474101","https://openalex.org/W2061459002"],"abstract_inverted_index":{"In":[0,131,324,374],"this":[1,132],"study,":[2],"understanding":[3],"of":[4,12,31,40,55,72,110,169,175,178,186,196,206,221,275,302,330,340,421,444,460,465,471],"the":[5,38,70,80,90,167,173,179,183,202,214,219,227,230,273,278,284,315,325,347,360,365,402,419,425,479],"central":[6],"metabolism":[7],"was":[8,135,161,310,493],"improved":[9,53,296,311],"by":[10,27,142,149,171,218,225,236,312,394,448,495],"quantification":[11],"metabolite":[13],"concentrations,":[14],"enzyme":[15],"activities,":[16],"protein":[17],"abundances,":[18],"and":[19,105,145,151,235,259,282,332,337,353,387,499],"gene":[20,81],"transcript":[21],"concentrations.":[22,439],"Intracellular":[23],"fluxes":[24],"were":[25,35,288,334,343],"estimated":[26],"applying":[28],"stoichiometric":[29],"models":[30],"metabolism.":[32],"The":[33],"methods":[34],"applied":[36],"in":[37,44,76,101,137,163,246,255,272,277,283,290,305,314,346,359,364,424,450,453,478,488],"study":[39,133,192],"yeast":[41],"Saccharomyces":[42],"cerevisiae":[43,115,377],"two":[45,138],"separate":[46],"projects.":[47],"A":[48,66],"xylose":[49,291,297],"project":[50,68],"aimed":[51],"at":[52],"utilization":[54,184,204,298],"D-xylose":[56,98,120,126,134,143,170,187,207,222,370],"as":[57],"a":[58,77,122,194,375,409,476,489],"substrate":[59],"for,":[60],"e.g.,":[61],"producing":[62],"biomaterial-based":[63],"fuel":[64],"ethanol.":[65],"mannose":[67,437],"studied":[69],"production":[71],"GDP-mannose":[73],"from":[74,153],"D-mannose":[75,386,397,417,431,485],"strain":[78,367,378,412,428],"lacking":[79],"for":[82,201,213,405],"phosphomannose":[83],"isomerase":[84,392,446],"(PMI40":[85],"deletion).":[86],"Hexose,":[87],"D-glucose":[88,388,406],"is":[89,99,116,127,389,401,455],"only":[91],"sugar":[92],"more":[93,356],"abundant":[94],"than":[95,363],"pentose":[96,232,279,326],"D-xylose.":[97,323],"common":[100],"hardwoods":[102],"(e.g.":[103],"birch)":[104],"crop":[106],"residues":[107],"(ca.":[108],"25%":[109],"dry":[111],"weight).":[112],"However,":[113,249,423],"S.":[114,376],"unable":[117],"to":[118,129,165,223,241,243,398,434,457,469,475],"utilize":[119],"without":[121,385],"recombinant":[123,181],"pathway":[124,182,234,328,404],"where":[125],"converted":[128,136],"D-xylulose.":[130,176],"steps":[139],"via":[140],"xylitol:":[141],"reductase":[144],"xylitol":[146],"dehydrogenase":[147],"encoded":[148,393],"XYL1":[150],"XYL2":[152],"Pichia":[154],"stipitis,":[155],"respectively.":[156],"Additionally,":[157],"endogenous":[158],"xylulokinase":[159],"(XKS1)":[160],"overexpressed":[162],"order":[164],"increase":[166],"consumption":[168],"enhancing":[172],"phosphorylation":[174],"Despite":[177],"functional":[180],"rates":[185,205],"still":[188],"remained":[189],"low.":[190],"This":[191],"proposes":[193],"set":[195],"limitations":[197,271],"that":[198],"are":[199],"responsible":[200],"low":[203],"under":[208,319,371],"microaerobic":[209],"conditions.":[210,373],"Cells":[211],"compensated":[212],"cofactor":[215],"imbalance,":[216],"caused":[217],"conversion":[220],"D-xylulose,":[224],"increasing":[226],"flux":[228,262],"through":[229,263],"oxidative":[231,247,268],"phosphate":[233,280,327],"shuttling":[237],"NADH":[238,251],"redox":[239],"potential":[240],"mitochondrion":[242],"be":[244],"oxidized":[245],"phosphorylation.":[248,269],"mitochondrial":[250],"inhibits":[252],"citrate":[253],"synthase":[254],"citric":[256,264,285],"acid":[257,265,286],"cycle,":[258],"consequently":[260,338],"lower":[261],"cycle":[266,287,498],"limits":[267],"Further,":[270],"uptake":[274],"D-xylose,":[276,303,309],"pathway,":[281],"alleviated":[289],"chemostat":[292,316,348,361],"isolates":[293,317,362],"with":[294,307,379],"three-fold":[295],"rates.":[299],"Uptake":[300],"rate":[301],"assayed":[304],"vitro":[306],"radioactive":[308],"60%":[313],"grown":[318,368],"aerobic":[320,372],"conditions":[321],"on":[322,369,383],"activities":[329],"transketolase":[331],"transaldolase":[333],"increased":[335,429,435,483],"two-fold,":[336],"concentrations":[339,432,486],"their":[341],"substrates":[342],"decreased":[344],"two-fold":[345],"isolates.":[349],"Finally,":[350],"less":[351],"pyruvate":[352],"citrate,":[354],"but":[355],"malate":[357],"accumulated":[358],"original":[366],"PMI40":[380,395,410,426],"deletion,":[381],"growth":[382,501],"media":[384],"disabled.":[390],"Phosphomannose":[391],"connects":[396],"glycolysis,":[399,451],"which":[400,452,492],"main":[403],"utilization.":[407],"Hypothetically,":[408],"deletion":[411,427],"would":[413],"direct":[414],"all":[415],"its":[416],"into":[418],"biosynthesis":[420],"GDP-mannose.":[422],"initial":[430,484],"led":[433,474],"intracellular":[436],"6-phosphate":[438,441],"Mannose":[440],"inhibited":[442],"activity":[443],"phosphoglucose":[445,472],"(encoded":[447],"PGI1)":[449],"essence":[454],"equivalent":[456],"suppressed":[458],"expression":[459],"PGI1.":[461],"Subsequently,":[462],"reduced":[463],"availability":[464],"glycolysis":[466],"intermediates,":[467],"due":[468],"inhibition":[470],"isomerase,":[473],"decrease":[477],"glycolytic":[480],"flux.":[481],"Eventually,":[482],"resulted":[487],"starvation":[490],"response,":[491],"accompanied":[494],"slower":[496,500],"cell":[497],"rate.":[502]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}