{"id":"https://openalex.org/W2366705362","doi":null,"title":"Screen of Dominating Microbial Species Isolated From Tibetan Kefir Using an Integrated Approach of PCR-DGGE and Culture-Dependent Methods","display_name":"Screen of Dominating Microbial Species Isolated From Tibetan Kefir Using an Integrated Approach of PCR-DGGE and Culture-Dependent Methods","publication_year":2006,"publication_date":"2006-01-01","ids":{"openalex":"https://openalex.org/W2366705362","mag":"2366705362"},"language":"en","primary_location":{"id":"mag:2366705362","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-ZNYK200608016.htm","pdf_url":null,"source":{"id":"https://openalex.org/S4306529288","display_name":"Zhongguo nongye Kexue","issn_l":"0578-1752","issn":["0578-1752"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-20","russia-white-list-2"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo nongye Kexue","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100327158","display_name":"Jianzhong Zhou","orcid":"https://orcid.org/0000-0002-0009-4284","observed_orcids":["https://orcid.org/0000-0002-0009-4284"]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianzhong Zhou","raw_affiliation_strings":["College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095)"],"raw_orcid":"https://orcid.org/0000-0002-0009-4284","affiliations":[{"raw_affiliation_string":"College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095)","institution_ids":["https://openalex.org/I119454577"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066110650","display_name":"Mingsheng Dong","orcid":"https://orcid.org/0000-0001-6796-2018","observed_orcids":["https://orcid.org/0000-0001-6796-2018","https://orcid.org/0000-0002-7454-4516","https://orcid.org/0000-0003-2696-4281"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mingsheng Dong","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-6796-2018","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5026380979","display_name":"Jiang Han-hu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I119454577","display_name":"Nanjing Agricultural University","ror":"https://ror.org/05td3s095","country_code":"CN","type":"education","lineage":["https://openalex.org/I119454577"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiang Han-hu","raw_affiliation_strings":["College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095)","institution_ids":["https://openalex.org/I119454577"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4068,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.72170097,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10750","display_name":"Fermentation and Sensory Analysis","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactic-acid-bacteria","display_name":"lactic acid bacteria","score":0.8889999985694885},{"id":"https://openalex.org/keywords/yeast","display_name":"yeast","score":0.9190000295639038},{"id":"https://openalex.org/keywords/starter-culture-development","display_name":"starter culture development","score":0.7260000109672546},{"id":"https://openalex.org/keywords/leuconostoc-mesenteroides","display_name":"Leuconostoc mesenteroides","score":0.9559999704360962},{"id":"https://openalex.org/keywords/lactococcus-lactis","display_name":"Lactococcus lactis","score":0.9990000128746033},{"id":"https://openalex.org/keywords/lactobacillus-kefiri","display_name":"Lactobacillus kefiri","score":0.9739999771118164},{"id":"https://openalex.org/keywords/lacticaseibacillus-casei","display_name":"Lactobacillus casei","score":0.972000002861023},{"id":"https://openalex.org/keywords/kluyveromyces-marxianus","display_name":"Kluyveromyces marxianus","score":0.9959999918937683},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9869999885559082},{"id":"https://openalex.org/keywords/pcr-dgge","display_name":"PCR-DGGE","score":0.7200000286102295},{"id":"https://openalex.org/keywords/16s-rrna-gene","display_name":"16S rRNA gene","score":0.859000027179718},{"id":"https://openalex.org/keywords/26s-rdna","display_name":"26S rDNA","score":0.9359999895095825},{"id":"https://openalex.org/keywords/uncultured-bacteria","display_name":"uncultured bacteria","score":0.531000018119812}],"concepts":[{"id":"https://openalex.org/C2778072784","wikidata":"https://www.wikidata.org/wiki/Q185749","display_name":"Kefir","level":4,"score":0.8073952198028564},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7177875638008118},{"id":"https://openalex.org/C2776917750","wikidata":"https://www.wikidata.org/wiki/Q5222586","display_name":"Kluyveromyces marxianus","level":4,"score":0.6852349042892456},{"id":"https://openalex.org/C2777957631","wikidata":"https://www.wikidata.org/wiki/Q146954","display_name":"Leuconostoc mesenteroides","level":4,"score":0.6006184816360474},{"id":"https://openalex.org/C2777858148","wikidata":"https://www.wikidata.org/wiki/Q23048822","display_name":"Acetic acid bacteria","level":3,"score":0.5084463357925415},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.48793527483940125},{"id":"https://openalex.org/C2780206646","wikidata":"https://www.wikidata.org/wiki/Q1061596","display_name":"Lactobacillus","level":3,"score":0.48487529158592224},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.4605604112148285},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.4404652714729309},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.43901723623275757},{"id":"https://openalex.org/C2777176278","wikidata":"https://www.wikidata.org/wiki/Q557828","display_name":"Starter","level":2,"score":0.4128614068031311},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4026412069797516},{"id":"https://openalex.org/C2775920511","wikidata":"https://www.wikidata.org/wiki/Q161249","display_name":"Lactic acid","level":3,"score":0.25798559188842773},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.2171614170074463},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0700625479221344},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.06048884987831116}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2366705362","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-ZNYK200608016.htm","pdf_url":null,"source":{"id":"https://openalex.org/S4306529288","display_name":"Zhongguo nongye Kexue","issn_l":"0578-1752","issn":["0578-1752"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-20","russia-white-list-2"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo nongye Kexue","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger","score":0.40450000762939453}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1984131732","https://openalex.org/W2003332600","https://openalex.org/W2012298406","https://openalex.org/W2030537885","https://openalex.org/W2111858111","https://openalex.org/W2112763919","https://openalex.org/W2114167906","https://openalex.org/W2131988453","https://openalex.org/W2142735254","https://openalex.org/W2148014016","https://openalex.org/W2161393176","https://openalex.org/W2167669991"],"related_works":["https://openalex.org/W1966549219","https://openalex.org/W2371035600","https://openalex.org/W2376564620","https://openalex.org/W2127239903","https://openalex.org/W2066202193","https://openalex.org/W2389328071","https://openalex.org/W7217206976","https://openalex.org/W1978263195","https://openalex.org/W2050026551","https://openalex.org/W2092751558"],"abstract_inverted_index":{"\u3010Objective\u3011To":[0],"screen":[1],"dominating":[2,89,110],"microbial":[3,22,90,116,132],"species":[4,46,91],"isolated":[5,62],"from":[6],"Tibetan":[7,25,38,59,93,138],"Kefir":[8,139],"and":[9,13,65,72,80,101,106,130],"develop":[10],"pure-culture":[11,140],"starter.\u3010Method\u3011PCR-DGGE":[12],"traditional":[14],"culture-depending":[15],"methods":[16],"were":[17,40,63,75,95,109],"combined":[18],"to":[19],"examine":[20],"the":[21,30,88,135],"community":[23],"of":[24,33,37,54,58,77,92,137],"kefir.\u3010Result\u3011":[26],"Four":[27],"bands":[28,50],"in":[29,51,125],"DGGE":[31,52],"profiles":[32],"total":[34,55],"bacterial":[35,45,56],"DNA":[36,57],"kefir":[39,94],"not":[41,48],"recovered":[42],"by":[43,67],"cultivation.Conversely,two":[44],"did":[47],"exhibit":[49],"fingerprints":[53],"kefir.150":[60],"strains":[61],"screened":[64],"grouped":[66],"PCR-DGGE,eight":[68],"lactic":[69],"acid":[70],"bacteria":[71],"five":[73],"yeasts":[74],"obtained.Comparison":[76],"16S":[78],"rDNA(bacteria)":[79],"26S":[81],"rDNA(yeast)":[82],"partial":[83],"sequence":[84],"similarity":[85],"revealed":[86],"that":[87],"Leuconostoc":[96],"mesenteroides,Lactococcus":[97],"lactis,Lactobacillus":[98],"kefiri,Lactobacillus":[99],"casei":[100],"Kluyveromyces":[102],"marxianus,Saccharomyces":[103],"unisporus,Saccharomyces":[104],"cerevisiae":[105],"Candida":[107],"humilis":[108],"during":[111],"last":[112],"fermentation.\u3010Conclusion\u3011":[113],"An":[114],"effective":[115],"screening":[117],"technology":[118],"based":[119],"on":[120],"molecular":[121],"microbiology":[122],"was":[123],"established":[124],"this":[126],"study,providing":[127],"theoretical":[128],"basis":[129],"rich":[131],"resources":[133],"for":[134],"development":[136],"starter.":[141]},"counts_by_year":[],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2016-06-24T00:00:00"}