{"id":"https://openalex.org/W2364154173","doi":null,"title":"Quantitative RT-PCR for the expression of plasminogen activator inhibitor 1(PAI-1) in kidneys of aged rats","display_name":"Quantitative RT-PCR for the expression of plasminogen activator inhibitor 1(PAI-1) in kidneys of aged rats","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W2364154173","mag":"2364154173"},"language":"en","primary_location":{"id":"mag:2364154173","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZHLN200303016.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764874668","display_name":"Zhonghua laonian yixue zazhi","issn_l":"0254-9026","issn":["0254-9026"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua laonian yixue zazhi","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100441917","display_name":"Xiang 'Anthony' Chen","orcid":"https://orcid.org/0000-0002-2499-8261","observed_orcids":["https://orcid.org/0000-0002-2499-8261"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Xiang Chen","raw_affiliation_strings":["mei, ZHANG Li, LIU Hang,HOU Kai"],"raw_orcid":"https://orcid.org/0000-0002-2499-8261","affiliations":[{"raw_affiliation_string":"mei, ZHANG Li, LIU Hang,HOU Kai","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5100441917"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.16514381,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasminogen-activator-inhibitor-1","display_name":"plasminogen activator inhibitor-1","score":0.996999979019165},{"id":"https://openalex.org/keywords/pai-1-gene-expression","display_name":"PAI-1 gene expression","score":0.9890000224113464},{"id":"https://openalex.org/keywords/kidney","display_name":"kidney","score":0.8809999823570251},{"id":"https://openalex.org/keywords/aging","display_name":"aging","score":0.9580000042915344},{"id":"https://openalex.org/keywords/aged-rats","display_name":"aged rats","score":0.9100000262260437}],"concepts":[{"id":"https://openalex.org/C2780968160","wikidata":"https://www.wikidata.org/wiki/Q423692","display_name":"SYBR Green I","level":4,"score":0.7862042188644409},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.7546286582946777},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7013937830924988},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.6678526997566223},{"id":"https://openalex.org/C4692481","wikidata":"https://www.wikidata.org/wiki/Q169526","display_name":"Reverse transcription polymerase chain reaction","level":4,"score":0.5649197697639465},{"id":"https://openalex.org/C2779679481","wikidata":"https://www.wikidata.org/wiki/Q13581432","display_name":"Plasminogen activator","level":2,"score":0.5577265024185181},{"id":"https://openalex.org/C134792803","wikidata":"https://www.wikidata.org/wiki/Q424886","display_name":"Glyceraldehyde 3-phosphate dehydrogenase","level":4,"score":0.5207248330116272},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5196265578269958},{"id":"https://openalex.org/C33922130","wikidata":"https://www.wikidata.org/wiki/Q419232","display_name":"Housekeeping gene","level":4,"score":0.5014448165893555},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5004689693450928},{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.48242029547691345},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.46963950991630554},{"id":"https://openalex.org/C2780675426","wikidata":"https://www.wikidata.org/wiki/Q410485","display_name":"Plasminogen activator inhibitor-1","level":3,"score":0.46499764919281006},{"id":"https://openalex.org/C88045685","wikidata":"https://www.wikidata.org/wiki/Q174576","display_name":"Activator (genetics)","level":3,"score":0.44695624709129333},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3551032543182373},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.16093674302101135},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.1558454930782318}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2364154173","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZHLN200303016.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764874668","display_name":"Zhonghua laonian yixue zazhi","issn_l":"0254-9026","issn":["0254-9026"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4320800525","host_organization_name":"Chinese Medical Association","host_organization_lineage":["https://openalex.org/P4320800525"],"host_organization_lineage_names":["Chinese Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhonghua laonian yixue zazhi","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8560000061988831,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W882314127","https://openalex.org/W2389124646","https://openalex.org/W298709328","https://openalex.org/W996762396","https://openalex.org/W1991070683","https://openalex.org/W3032692310","https://openalex.org/W3192658431","https://openalex.org/W2085668533","https://openalex.org/W1982870383","https://openalex.org/W2467031052"],"abstract_inverted_index":{"Objective":[0],"To":[1],"determine":[2],"whether":[3],"quantitative":[4,149],"reverse":[5],"transcriptase":[6],"polymerase":[7],"chain":[8],"reaction":[9],"(RT":[10],"PCR)":[11],"with":[12,66,151],"SYBR":[13,67,152],"GREEN\u2160":[14,153],"fluorescence":[15,154],"is":[16,176],"a":[17,157],"reliable":[18,158],"and":[19,43,83],"flexible":[20],"method":[21,159],"for":[22,27,160],"detecting":[23],"the":[24,51,125,167,181],"gene":[25,57,95,99,111,168],"expression":[26,58,112,175],"those":[28,161],"rarely":[29,162],"available":[30,163],"tissues.":[31],"Methods":[32],"RNA":[33],"was":[34,59,73,101,118],"isolated":[35],"from":[36],"kidneys":[37,114,179],"of":[38,50,92,115,124,172,180,186],"24":[39],"month":[40],"old":[41,46],"rats(6)":[42],"3":[44],"mouth":[45],"rats(6).":[47],"The":[48,108,170],"level":[49,171],"PAI":[52,93,109,173],"1(plasminogen":[53],"activator":[54],"inhibitor":[55],"I)":[56],"measured":[60],"using":[61],"real":[62],"time":[63],"RT":[64],"PCR":[65,106,144,150],"GREEN\u2160.":[68],"Glyceraldehyde":[69],"phosphate":[70],"dehydrogenase":[71],"(G3PDH)":[72],"used":[74],"as":[75],"external":[76],"control.":[77],"Results":[78],"Standard":[79],"curves":[80],"were":[81],"linear":[82],"showed":[84],"high":[85],"correlations":[86],"(R":[87],"2":[88],"0":[89,140],"99).":[90],"Ratio":[91],"1":[94,110,174],"copies":[96,100],"to":[97,104,122,165],"G3PDH":[98],"calculated":[102],"according":[103],"specific":[105],"products.":[107],"in":[113,178],"aged":[116,182],"rats":[117,127,183],"significantly":[119],"increased":[120],"compared":[121],"that":[123,185],"young":[126,187],"\u3014(1":[128],"27\u00b10":[129],"90)\u00d710":[130],"-3":[131,137],"vs":[132],"(3":[133],"84\u00b12":[134],"00)\u00d7":[135],"10":[136],",":[138],"P":[139],"05).":[141],"Conclusions":[142],"If":[143],"conditions":[145],"are":[146],"controlled":[147],"strictly,":[148],"can":[155],"be":[156],"tissues":[164],"detect":[166],"expression.":[169],"higher":[177],"than":[184],"rats.":[188]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}