{"id":"https://openalex.org/W2361661263","doi":null,"title":"Isolation and identification of CD4~+CD25~+ regulatory T cells","display_name":"Isolation and identification of CD4~+CD25~+ regulatory T cells","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W2361661263","mag":"2361661263"},"language":"en","primary_location":{"id":"mag:2361661263","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-JLYB200903004.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764879260","display_name":"Yixue yanjiusheng xuebao","issn_l":"1008-8199","issn":["1008-8199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4361729665","host_organization_name":"Nanjing General of Nanjing Military Region","host_organization_lineage":["https://openalex.org/P4361729665"],"host_organization_lineage_names":["Nanjing General of Nanjing Military Region"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Yixue yanjiusheng xuebao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021922143","display_name":"Lili Ding","orcid":"https://orcid.org/0000-0003-2345-3726","observed_orcids":["https://orcid.org/0000-0003-2345-3726"]},"institutions":[{"id":"https://openalex.org/I4210158926","display_name":"Xijing Hospital","ror":"https://ror.org/05cqe9350","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210158926"]},{"id":"https://openalex.org/I9916479","display_name":"Air Force Medical University","ror":"https://ror.org/00ms48f15","country_code":"CN","type":"education","lineage":["https://openalex.org/I9916479"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Lili Ding","raw_affiliation_strings":["Department of Ophthalmology,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China)"],"raw_orcid":"https://orcid.org/0000-0003-2345-3726","affiliations":[{"raw_affiliation_string":"Department of Ophthalmology,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,Shaanxi,China)","institution_ids":["https://openalex.org/I4210158926","https://openalex.org/I9916479"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5021922143"],"corresponding_institution_ids":["https://openalex.org/I4210158926","https://openalex.org/I9916479"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.32358715,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10469","display_name":"Psoriasis: Treatment and Pathogenesis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11510","display_name":"Reproductive System and Pregnancy","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/regulatory-t-cells","display_name":"regulatory T cells","score":0.9950000047683716},{"id":"https://openalex.org/keywords/cd4-cd25-regulatory-t-cells","display_name":"CD4+CD25+ T cells","score":0.9950000047683716},{"id":"https://openalex.org/keywords/foxp3","display_name":"Foxp3","score":0.9610000252723694},{"id":"https://openalex.org/keywords/ctla-4","display_name":"CTLA-4","score":0.996999979019165},{"id":"https://openalex.org/keywords/cd127","display_name":"CD127","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cd69","display_name":"CD69","score":0.8629999756813049},{"id":"https://openalex.org/keywords/cd28","display_name":"CD28","score":0.7940000295639038},{"id":"https://openalex.org/keywords/treg-isolation","display_name":"Treg isolation","score":0.5899999737739563},{"id":"https://openalex.org/keywords/magnetic-activated-cell-sorting","display_name":"magnetic cell separation","score":0.8679999709129333},{"id":"https://openalex.org/keywords/splenocytes","display_name":"splenocytes","score":0.8209999799728394},{"id":"https://openalex.org/keywords/treg-phenotype","display_name":"Treg phenotype","score":0.503000020980835},{"id":"https://openalex.org/keywords/treg-function","display_name":"Treg function","score":0.8180000185966492},{"id":"https://openalex.org/keywords/immunosuppression","display_name":"immunosuppression","score":0.5619999766349792},{"id":"https://openalex.org/keywords/treg-depletion","display_name":"Treg depletion","score":0.3409999907016754},{"id":"https://openalex.org/keywords/treg-reconstitution","display_name":"Treg reconstitution","score":0.4339999854564667},{"id":"https://openalex.org/keywords/adoptive-immunotherapy","display_name":"adoptive immunotherapy","score":0.39800000190734863}],"concepts":[{"id":"https://openalex.org/C79484868","wikidata":"https://www.wikidata.org/wiki/Q5971673","display_name":"IL-2 receptor","level":4,"score":0.833541750907898},{"id":"https://openalex.org/C2779727006","wikidata":"https://www.wikidata.org/wiki/Q426188","display_name":"FOXP3","level":3,"score":0.6419613361358643},{"id":"https://openalex.org/C148125776","wikidata":"https://www.wikidata.org/wiki/Q1622348","display_name":"CD28","level":4,"score":0.5449574589729309},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5058919787406921},{"id":"https://openalex.org/C154317977","wikidata":"https://www.wikidata.org/wiki/Q376266","display_name":"Cytotoxic T cell","level":3,"score":0.4982950687408447},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.495583176612854},{"id":"https://openalex.org/C129374314","wikidata":"https://www.wikidata.org/wiki/Q909381","display_name":"Interleukin 21","level":4,"score":0.45396339893341064},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42880886793136597},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.42483580112457275},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.41354838013648987},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3773849308490753},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.35118961334228516},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.25407442450523376},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.21780866384506226},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.21085211634635925},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.18333446979522705},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0759952962398529}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2361661263","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-JLYB200903004.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764879260","display_name":"Yixue yanjiusheng xuebao","issn_l":"1008-8199","issn":["1008-8199"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4361729665","host_organization_name":"Nanjing General of Nanjing Military Region","host_organization_lineage":["https://openalex.org/P4361729665"],"host_organization_lineage_names":["Nanjing General of Nanjing Military Region"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Yixue yanjiusheng xuebao","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2379648434","https://openalex.org/W2347333974","https://openalex.org/W182023681","https://openalex.org/W2351491176","https://openalex.org/W10522450","https://openalex.org/W3148556801","https://openalex.org/W336108072","https://openalex.org/W2378481175","https://openalex.org/W3030270614","https://openalex.org/W885146802"],"abstract_inverted_index":{"Objective:Regulatory":[0],"T":[1,20,114,150,155,222],"cells":[2,21,43,103,156,170,186,206,223,281],"are":[3],"the":[4,14,26,63,136,148,169,176,226],"hotpoint":[5],"of":[6,16,31,38,40,86,95,107,132,168,194,205,229,240,251,287],"immunology":[7],"for":[8],"recent":[9],"years.":[10],"In":[11],"this":[12],"study":[13],"preparation":[15],"high":[17],"purity":[18],"regulatory":[19],"(Treg)":[22],"was":[23,34,178,181,198],"investisated":[24],"and":[25,28,62,69,129,174,273,284],"phenotypic":[27,283],"functional":[29,285],"characteristics":[30,286],"innate":[32,141,233,288],"Treg":[33],"identified.":[35],"Methods:The":[36],"phenotype":[37],"3-subpopulation":[39],"CD4+/CD4+CD25+/":[41],"CD4+CD25-T":[42],"derived":[44],"from":[45,159,261],"normal":[46],"murine":[47,160,262],"SPL":[48,263],"were":[49,54,73,144,157,276],"analyzed":[50],"by":[51,56,75,83,162,184,200,237,265],"FACS.":[52,76],"CD+CD25+Tregs":[53],"isolated":[55,158,220,260],"two-step":[57],"magnetic":[58,164,267],"cell":[59,80,151,165,268],"sorting":[60],"(MACS)":[61],"Treg-specific":[64],"transcription":[65],"factor":[66,123],"Foxp3,intracellular":[67],"CTLA-4":[68,197],"surface":[70,99,255],"antigen":[71,117],"CD127":[72],"identified":[74],"Results:\u2460These":[77],"three":[78,232],"CD4+T":[79,280],"populations":[81,235],"defined":[82,236],"varying":[84,238],"levels":[85,106,131,239],"CD25":[87,241],"expression":[88,242],"exhibited":[89,245],"marked":[90,246],"differences":[91,247],"in":[92,207,243,248,279],"a":[93,201,249],"number":[94,250],"some":[96],"intracellular":[97,253],"or":[98,138,189,216,254],"antigens.":[100],"The":[101,219],"CD4+CD25+T":[102,185],"expressed":[104,171,183,199,225],"higher":[105,203],"forkhead":[108],"box":[109],"P3":[110],"(":[111,119],"Foxp3":[112,180,195],"),cytotoxic":[113],"lymphocyte":[115],"associated":[116],"4":[118],"CTLA-4),glucocorticoid-induced":[120],"tumor":[121],"necrosis":[122],"receptor":[124],"(GITR":[125],")and":[126],"CD69":[127],"(P0.01)":[128],"lower":[130],"CD28":[133],"(P0.05)":[134],"than":[135],"CD4+":[137,188,214],"CD4+CD25-subsets.":[139],"These":[140],"CD4+CD25+":[142,154,208,221],"Tregs":[143],"typically":[145],"confined":[146],"within":[147],"CD45RBlow":[149],"subset.":[152],"\u2461After":[153],"splenocytes":[161],"2-step":[163,266],"sorting,90.19%":[166],"(range,87.29%\uff5e92.04%)":[167],"both":[172],"CD4":[173],"CD25,and":[175],"yield":[177],"1%\uff5e1.2%.":[179],"preferentially":[182],"(66.6\u00b11.033%),whereas":[187],"CD4+CD25-subpopulation":[190],"contained":[191],"low":[192,227],"level":[193],"(23.97\u00b11.997%,16.78\u00b13.24%).":[196],"significantly":[202],"proportion":[204],"population":[209],"(77.37\u00b11.67%)":[210],"as":[211,257],"compared":[212],"to":[213],"(21.97\u00b12.39%)":[215],"CD4+CD25-(18.04\u00b13.16)":[217],"subsets.":[218],"also":[224],"intensity":[228],"CD127.":[230],"Conclusion:These":[231],"CD4+T-cell":[234],"mice":[244],"other":[252],"antigens,such":[256],"Foxp3,CTLA-4,GITR,CD45RB,CD69,CD28.":[258],"Cells":[259],"products":[264],"separation":[269],"protocol":[270],"(negative":[271],"selection":[272],"positive":[274],"selection)":[275],"highly":[277],"enriched":[278],"with":[282],"CD4+CD25+Treg.":[289]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}
