{"id":"https://openalex.org/W2356059635","doi":null,"title":"Protein spot train in 2-DE gels for bacterial proteome:a primary research","display_name":"Protein spot train in 2-DE gels for bacterial proteome:a primary research","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2356059635","mag":"2356059635"},"language":"en","primary_location":{"id":"mag:2356059635","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-JSYX201101015.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764605507","display_name":"Junshi yixue","issn_l":"1674-9960","issn":["1674-9960"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Junshi yixue","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108575320","display_name":"Hu Wei","orcid":"https://orcid.org/0009-0008-3369-229X","observed_orcids":["https://orcid.org/0009-0008-3369-229X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Hu Wei","raw_affiliation_strings":["1,2,ZHU Li2,SHANG Na2,WANG Xue-fang2,XU Hui-jie2,FENG Er-ling2,WEI Hua1*,WANG Heng-liang1,2*"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1,2,ZHU Li2,SHANG Na2,WANG Xue-fang2,XU Hui-jie2,FENG Er-ling2,WEI Hua1*,WANG Heng-liang1,2*","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5108575320"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/two-dimensional-electrophoresis","display_name":"two-dimensional gel electrophoresis","score":0.7239999771118164},{"id":"https://openalex.org/keywords/bacterial-proteome","display_name":"bacterial proteome","score":0.9879999756813049},{"id":"https://openalex.org/keywords/protein-sample-preparation","display_name":"protein sample preparation","score":0.6129999756813049},{"id":"https://openalex.org/keywords/protein-modification","display_name":"protein modification","score":0.5649999976158142},{"id":"https://openalex.org/keywords/acidic-proteins","display_name":"acidic proteins","score":0.7820000052452087},{"id":"https://openalex.org/keywords/maldi-tof-tof-mass-spectrometry","display_name":"MALDI-TOF/TOF mass spectrometry","score":0.8330000042915344},{"id":"https://openalex.org/keywords/protein-spot-identification","display_name":"protein spot identification","score":0.5139999985694885}],"concepts":[{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.8808609247207642},{"id":"https://openalex.org/C2781255879","wikidata":"https://www.wikidata.org/wiki/Q7580087","display_name":"Spots","level":2,"score":0.8407844305038452},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6767145991325378},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.5713971257209778},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.5644773840904236},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5066218972206116},{"id":"https://openalex.org/C2993175405","wikidata":"https://www.wikidata.org/wiki/Q8054","display_name":"Bacterial protein","level":3,"score":0.4434340298175812},{"id":"https://openalex.org/C190431759","wikidata":"https://www.wikidata.org/wiki/Q6710242","display_name":"Lysis buffer","level":3,"score":0.4351423680782318},{"id":"https://openalex.org/C47793336","wikidata":"https://www.wikidata.org/wiki/Q4688061","display_name":"Two-dimensional gel electrophoresis","level":4,"score":0.41943103075027466},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.3523528277873993},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2660832405090332},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.11157652735710144},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2356059635","doi":null,"is_oa":false,"landing_page_url":"http://en.cnki.com.cn/Article_en/CJFDTOTAL-JSYX201101015.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764605507","display_name":"Junshi yixue","issn_l":"1674-9960","issn":["1674-9960"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Junshi yixue","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2395691567","https://openalex.org/W3030409512","https://openalex.org/W4309515236","https://openalex.org/W2379821484","https://openalex.org/W4711536","https://openalex.org/W2023203611","https://openalex.org/W2036792864","https://openalex.org/W2420133998","https://openalex.org/W2076905068","https://openalex.org/W2077565457"],"abstract_inverted_index":{"Objective":[0],"To":[1],"find":[2],"the":[3,28,43,57,76,84,90],"possible":[4],"reason":[5],"for":[6,31],"making":[7,32],"spot":[8,33],"train":[9,38,53,71],"in":[10,62,89],"2-DE":[11,17],"gels":[12,18,44],"of":[13,83,93],"bacterial":[14],"proteome.Methods":[15],"The":[16],"with":[19,114],"different":[20],"sample":[21],"preparation":[22],"methods":[23],"were":[24,39,60,72],"compared":[25],"to":[26,74,100,107],"discover":[27],"key":[29],"factor":[30],"train.The":[34],"spots":[35,68],"forming":[36],"a":[37,101],"cut":[40],"off":[41],"form":[42],"and":[45],"digested":[46],"by":[47,50,79],"trypsin,then":[48],"analyzed":[49],"MALDI-TOF/TOF.Results":[51],"Spot":[52],"would":[54,98],"appear":[55],"when":[56,109],"protein":[58,78,95],"samples":[59],"boiled":[61],"lysis":[63],"buffer":[64],"containing":[65],"0.5%":[66],"SDS.The":[67],"on":[69],"one":[70],"identified":[73],"be":[75],"same":[77],"MALDI-TOF/TOF.No":[80],"significant":[81],"shift":[82],"mass":[85],"peaks":[86],"was":[87],"observed":[88],"PMF":[91],"spectra":[92],"these":[94],"spots.Conclusion":[96],"SDS":[97],"lead":[99],"certain":[102],"modification":[103],"or":[104],"tight":[105],"binding":[106],"proteins":[108,113],"boiled,especially":[110],"those":[111],"acidic":[112],"large":[115],"relative":[116],"molecular":[117],"mass.":[118]},"counts_by_year":[],"updated_date":"2026-09-21T07:43:02.447757","created_date":"2016-06-24T00:00:00"}