{"id":"https://openalex.org/W2346339239","doi":null,"title":"Cloning and expression of synthesis fragment of Mycoplasma suis MSG1 gene.","display_name":"Cloning and expression of synthesis fragment of Mycoplasma suis MSG1 gene.","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W2346339239","mag":"2346339239"},"language":"en","primary_location":{"id":"mag:2346339239","doi":null,"is_oa":false,"landing_page_url":"https://www.cabdirect.org/abstracts/20103355716.html","pdf_url":null,"source":{"id":"https://openalex.org/S2764620810","display_name":"Zhongguo shouyi xuebao","issn_l":"1005-4545","issn":["1005-4545"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696411","host_organization_name":"Jilin University","host_organization_lineage":["https://openalex.org/P4322696411"],"host_organization_lineage_names":["Jilin University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo shouyi xuebao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100736293","display_name":"Tiantian Chen","orcid":"https://orcid.org/0000-0002-6021-724X","observed_orcids":["https://orcid.org/0000-0002-6021-724X"]},"institutions":[{"id":"https://openalex.org/I74485261","display_name":"Shandong Agricultural University","ror":"https://ror.org/02ke8fw32","country_code":"CN","type":"education","lineage":["https://openalex.org/I74485261"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tiantian Chen","raw_affiliation_strings":["College of Veterinary Medicine and Animal Science,Shandong Agricultural University,Tai\u2032an,Shandong 271018,China)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Veterinary Medicine and Animal Science,Shandong Agricultural University,Tai\u2032an,Shandong 271018,China)","institution_ids":["https://openalex.org/I74485261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066122148","display_name":"Jianzhu Liu","orcid":"https://orcid.org/0000-0003-3634-0712","observed_orcids":["https://orcid.org/0000-0003-3634-0712"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jianzhu Liu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3634-0712","affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Dong Zhou","orcid":"https://orcid.org/0000-0001-7101-4125","observed_orcids":["https://orcid.org/0000-0001-7101-4125"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dong Zhou","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7101-4125","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032317764","display_name":"Haitao Liu","orcid":"https://orcid.org/0000-0002-3757-779X","observed_orcids":["https://orcid.org/0000-0002-3757-779X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Haitao Liu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089865552","display_name":"Ziqiang Cheng","orcid":"https://orcid.org/0000-0003-4323-2541","observed_orcids":["https://orcid.org/0000-0003-4323-2541"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ziqiang Cheng","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4323-2541","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031096137","display_name":"Huijun Guo","orcid":"https://orcid.org/0000-0001-9407-7479","observed_orcids":["https://orcid.org/0000-0001-9407-7479"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Huijun Guo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9407-7479","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110105415","display_name":"Wang ZhenYong","orcid":"https://orcid.org/0000-0003-1904-4372","observed_orcids":["https://orcid.org/0000-0003-1904-4372"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wang ZhenYong","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"Zhang Li","orcid":"https://orcid.org/0000-0001-9242-0763","observed_orcids":["https://orcid.org/0000-0001-9242-0763"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhang Li","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9242-0763","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5027876470","display_name":"Tongjie Chai","orcid":"https://orcid.org/0009-0008-1075-5251","observed_orcids":["https://orcid.org/0009-0008-1075-5251"]},"institutions":[{"id":"https://openalex.org/I74485261","display_name":"Shandong Agricultural University","ror":"https://ror.org/02ke8fw32","country_code":"CN","type":"education","lineage":["https://openalex.org/I74485261"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tongjie Chai","raw_affiliation_strings":["College of Veterinary Medicine and Animal Science,Shandong Agricultural University,Tai\u2032an,Shandong 271018,China)"],"raw_orcid":"https://orcid.org/0009-0008-1075-5251","affiliations":[{"raw_affiliation_string":"College of Veterinary Medicine and Animal Science,Shandong Agricultural University,Tai\u2032an,Shandong 271018,China)","institution_ids":["https://openalex.org/I74485261"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"30","issue":"7","first_page":"945","last_page":"948"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mycoplasma-suis","display_name":"Mycoplasma suis","score":0.9940000176429749},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8740000128746033},{"id":"https://openalex.org/keywords/e-coli-bl21","display_name":"E. coli BL21","score":0.6800000071525574},{"id":"https://openalex.org/keywords/iptg-induction","display_name":"IPTG induction","score":0.8820000290870667},{"id":"https://openalex.org/keywords/fusion-protein","display_name":"fusion protein","score":0.6600000262260437},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.503000020980835}],"concepts":[{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.8026766777038574},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7786740064620972},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7212252020835876},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.674188494682312},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.5916391611099243},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5669522881507874},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.5487988591194153},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.49118730425834656},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.48993605375289917},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.47570735216140747},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.4717696011066437},{"id":"https://openalex.org/C142372107","wikidata":"https://www.wikidata.org/wiki/Q309871","display_name":"pUC19","level":4,"score":0.436831533908844},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.29566705226898193},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22835421562194824},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2346339239","doi":null,"is_oa":false,"landing_page_url":"https://www.cabdirect.org/abstracts/20103355716.html","pdf_url":null,"source":{"id":"https://openalex.org/S2764620810","display_name":"Zhongguo shouyi xuebao","issn_l":"1005-4545","issn":["1005-4545"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322696411","host_organization_name":"Jilin University","host_organization_lineage":["https://openalex.org/P4322696411"],"host_organization_lineage_names":["Jilin University"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Zhongguo shouyi xuebao","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2351553673","https://openalex.org/W892647129","https://openalex.org/W2608407531","https://openalex.org/W2350580134","https://openalex.org/W2067067615","https://openalex.org/W2352154893","https://openalex.org/W2977927745","https://openalex.org/W2386887076","https://openalex.org/W2376382951","https://openalex.org/W302460324"],"abstract_inverted_index":{"To":[0],"synthesize":[1,46],"the":[2,10,13,20,24,30,47,51,55,71,81,85,94,110],"fragment":[3,75],"of":[4,12,23,33,43,100],"Mycoplasma":[5],"suis":[6],"MSG1":[7,52,73],"gene,and":[8],"improve":[9],"expression":[11,77],"aim":[14],"protein":[15,102,117],"in":[16,29,126],"Escherichia":[17],"coli.Base":[18],"on":[19],"gene":[21,48],"sequence":[22],"AM407404":[25],"(544-942)":[26],"which":[27,49],"registered":[28],"GenBank,six":[31],"pairs":[32],"oligonucleotides":[34],"were":[35],"synthesized":[36],"by":[37,61,69,83,105,124],"using":[38],"E.coli":[39,90],"biased":[40],"codons.By":[41],"means":[42],"PAS":[44],"to":[45],"encoding":[50],"protein,cloned":[53],"into":[54,76,89],"clone":[56],"vector":[57,78],"pjet1.2/blunt":[58],"and":[59,80,92],"confirmed":[60],"DNA":[62,74],"sequencing.The":[63],"recombinant":[64,86,95,111],"plasmid":[65,87,112],"pET-28c/MSG1":[66,88],"was":[67,103,113,122],"constructed":[68,114],"inserting":[70],"purified":[72],"pET-28c":[79],"expressed":[82],"transforming":[84],"BL21":[91],"induced":[93,123],"with":[96,118],"IPTG.The":[97],"expressing":[98],"situation":[99],"fusion":[101],"analyzed":[104],"SDS-PAGE.The":[106],"result":[107],"indicated":[108],"that":[109],"successfully,and":[115],"a":[116],"about":[119],"16":[120],"000":[121],"IPTG":[125],"SDS-PAGE.":[127]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2016-06-24T00:00:00"}