{"id":"https://openalex.org/W2345895473","doi":null,"title":"Novel Mechanisms Governing Autoregulation of the Src Family Kinase Fyn and its Crosstalk with Protein Kinase A","display_name":"Novel Mechanisms Governing Autoregulation of the Src Family Kinase Fyn and its Crosstalk with Protein Kinase A","publication_year":2016,"publication_date":"2016-01-01","ids":{"openalex":"https://openalex.org/W2345895473","mag":"2345895473"},"language":"en","primary_location":{"id":"pmh:oai:scholarworks.uvm.edu:graddis-1591","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uvm.edu/graddis/592","pdf_url":null,"source":{"id":"https://openalex.org/S4377196529","display_name":"ScholarWorks -A service of University of Vermont Libraries (University of Vermont)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I111236770","host_organization_name":"University of Vermont","host_organization_lineage":["https://openalex.org/I111236770"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate College Dissertations and Theses","raw_type":"text"},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083106015","display_name":"Marion E. Weir","orcid":"https://orcid.org/0000-0002-6144-0531","observed_orcids":["https://orcid.org/0000-0002-6144-0531"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Marion E. Weir","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-6144-0531","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5083106015"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.02235833,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12104","display_name":"Protein Tyrosine Phosphatases","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13779","display_name":"Cancer Mechanisms and Therapy","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fyn","display_name":"Fyn","score":0.8679999709129333},{"id":"https://openalex.org/keywords/src-family-kinases","display_name":"Src family kinases","score":0.9380000233650208},{"id":"https://openalex.org/keywords/protein-kinase-a","display_name":"protein kinase A","score":0.9620000123977661},{"id":"https://openalex.org/keywords/kinase-autophosphorylation","display_name":"kinase autophosphorylation","score":0.878000020980835},{"id":"https://openalex.org/keywords/tyrosine-phosphorylation-sites","display_name":"tyrosine phosphorylation sites","score":0.953000009059906},{"id":"https://openalex.org/keywords/sh2-domain","display_name":"SH2 domain","score":0.8180000185966492},{"id":"https://openalex.org/keywords/c-terminal-src-kinase","display_name":"C-terminal Src kinase","score":0.8059999942779541},{"id":"https://openalex.org/keywords/pka-activation","display_name":"PKA activation","score":0.5410000085830688},{"id":"https://openalex.org/keywords/protein-phosphorylation","display_name":"protein phosphorylation","score":0.3179999887943268},{"id":"https://openalex.org/keywords/signaling-pathways","display_name":"signaling pathways","score":0.453000009059906},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.49300000071525574},{"id":"https://openalex.org/keywords/cell-migration","display_name":"cell migration","score":0.7919999957084656},{"id":"https://openalex.org/keywords/cell-adhesion","display_name":"cell adhesion","score":0.6159999966621399}],"concepts":[{"id":"https://openalex.org/C108636557","wikidata":"https://www.wikidata.org/wiki/Q21109354","display_name":"Proto-oncogene tyrosine-protein kinase Src","level":3,"score":0.6188321709632874},{"id":"https://openalex.org/C169822122","wikidata":"https://www.wikidata.org/wiki/Q230187","display_name":"Crosstalk","level":2,"score":0.601559042930603},{"id":"https://openalex.org/C37686147","wikidata":"https://www.wikidata.org/wiki/Q423628","display_name":"FYN","level":4,"score":0.5537369251251221},{"id":"https://openalex.org/C2779976819","wikidata":"https://www.wikidata.org/wiki/Q7582406","display_name":"Src family kinase","level":4,"score":0.5359753966331482},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5253723859786987},{"id":"https://openalex.org/C111774379","wikidata":"https://www.wikidata.org/wiki/Q1364253","display_name":"Autoregulation","level":3,"score":0.5169198513031006},{"id":"https://openalex.org/C184235292","wikidata":"https://www.wikidata.org/wiki/Q421851","display_name":"Kinase","level":2,"score":0.5123277902603149},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31942856311798096},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.14817330241203308},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.11396574974060059},{"id":"https://openalex.org/C24326235","wikidata":"https://www.wikidata.org/wiki/Q126095","display_name":"Electronic engineering","level":1,"score":0.05867737531661987},{"id":"https://openalex.org/C84393581","wikidata":"https://www.wikidata.org/wiki/Q82642","display_name":"Blood pressure","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholarworks.uvm.edu:graddis-1591","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uvm.edu/graddis/592","pdf_url":null,"source":{"id":"https://openalex.org/S4377196529","display_name":"ScholarWorks -A service of University of Vermont Libraries (University of Vermont)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I111236770","host_organization_name":"University of Vermont","host_organization_lineage":["https://openalex.org/I111236770"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate College Dissertations and Theses","raw_type":"text"},{"id":"mag:2345895473","doi":null,"is_oa":false,"landing_page_url":"https://scholarworks.uvm.edu/graddis/592/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2308778564","https://openalex.org/W1147378958","https://openalex.org/W2309817607","https://openalex.org/W2136929338","https://openalex.org/W2105356859","https://openalex.org/W4249092084","https://openalex.org/W1785533687","https://openalex.org/W2775135720","https://openalex.org/W2057415995","https://openalex.org/W2073598808"],"abstract_inverted_index":{"ABSTRACT":[0],"Phosphorylation":[1,173],"is":[2,16,135,146,160,166,239,287,321],"a":[3,63,147,281],"post-translational":[4],"modification":[5],"important":[6,69,289],"for":[7,56,83,203,431],"regulating":[8,204,339],"protein":[9,12],"activity":[10,155,206,269],"and":[11,29,39,66,74,96,132,144,165,237,270,286,294,297,332,372,394,417,457],"binding":[13,272,377,405],"capacity.":[14],"It":[15,359],"used":[17],"in":[18,47,58,70,137,182,186,219,233,241,247,258,280,290,305,375,390,408],"many":[19,84,398],"different":[20],"signaling":[21,49,57,284,307],"pathways":[22,285],"within":[23,442],"the":[24,89,97,138,154,157,163,183,205,208,225,229,234,242,268,274,312,326,349,363,380,409,425,443],"cell.":[25],"Src":[26,113,170],"Family":[27,114],"Kinases":[28],"Protein":[30],"Kinase":[31,115],"A":[32],"(PKA)":[33],"are":[34,45,54,67,105,201],"two":[35,52,125,327],"prototyptical":[36],"non-receptor":[37],"tyrosine":[38,126,217],"serine/":[40],"threonine":[41],"kinases,":[42],"respectively,":[43],"which":[44,92,461],"found":[46,401],"canonical":[48],"pathways.":[50,308,328],"These":[51,421],"kinases":[53],"critical":[55,456],"essentially":[59],"every":[60],"cell":[61,72,291,444],"of":[62,88,156,174,207,216,228,273,283,351,354,365,382,387,411,427,440],"multicellular":[64],"organism,":[65],"particularly":[68,288],"development,":[71],"migration":[73],"proliferation.":[75],"Although":[76],"both":[77],"proteins":[78,104,399,414,441],"have":[79,299],"been":[80,119,278,301],"intensely":[81],"studied":[82],"decades,":[85],"an":[86,187],"understanding":[87,454],"molecular":[90],"mechanisms":[91],"govern":[93],"their":[94,404],"regulation":[95,98],"that":[99,152,212,260,319,323,362,424],"they":[100],"effect":[101],"on":[102,344,455],"other":[103],"still":[106],"being":[107],"elucidated.":[108],"Fyn,":[109],"like":[110],"its":[111,447],"related":[112],"members,":[116],"has":[117,194,277,316],"previously":[118],"shown":[120],"to":[121,178,180,303,369,373,402,406,433,445],"be":[122,304],"regulated":[123],"by":[124,168,460],"phosphorylation":[127,218,264],"events":[128],"at":[129],"residues":[130],"Y420":[131,134],"Y531.":[133],"located":[136,161,240],"kinase":[139,171,243,249],"(Src":[140],"Homology":[141],"1(SH1))":[142],"domain":[143,185],"it":[145,177],"highly-characterized":[148],"intermolecular":[149],"autophosphorylation":[150],"site":[151],"increases":[153],"kinase.":[158],"Y531":[159,175],"near":[162],"C-terminus":[164],"phosphorylated":[167],"C-terminal":[169],"(Csk).":[172],"allows":[176],"bind":[179],"R176":[181],"SH2":[184,235],"intramolecular":[188],"fashion.":[189],"In":[190],"this":[191,355],"conformation":[192],"Fyn":[193,220,263,296,331,366,428],"only":[195],"basal":[196],"activity.":[197,342],"Since":[198],"these":[199,261,345,451],"sites":[200,215,231,265],"essential":[202],"kinase,":[209],"we":[210,347],"hypothesized":[211,348,361],"four":[213],"novel":[214,230,262],"could":[221,367,429],"also":[222],"importantly":[223,435],"regulate":[224],"protein.":[226,275],"Three":[227],"lie":[232],"domain,":[236],"one":[238],"domain.":[244],"Mass":[245],"spectrometry,":[246,397],"vitro":[248],"assays,":[250],"as":[251,253],"well":[252],"western":[254],"blot":[255],"analysis":[256],"aided":[257],"uncovering":[259],"fine":[266],"tune":[267],"substrate":[271],"PKA":[276,298,333,370,376,407,432],"implicated":[279],"multitude":[282],"growth,":[292],"proliferation,":[293],"migration.":[295],"classically":[300],"considered":[302],"separate":[306],"However,":[309],"research":[310],"over":[311],"past":[313],"several":[314],"decades":[315],"provided":[317],"evidence":[318],"there":[320],"crosstalk":[322],"exists":[324],"between":[325],"The":[329],"SFK":[330],"can":[334],"phosphorylate":[335],"each":[336,340],"other,":[337],"thereby":[338],"other's":[341],"Based":[343],"data,":[346],"existence":[350],"downstream":[352],"effectors":[353],"relatively":[356],"uncharacterized":[357],"pathway.":[358],"was":[360],"presence":[364,410,426],"lead":[368],"activation":[371],"differences":[374],"partners.":[378],"Through":[379],"use":[381],"co-immunoprecipitations,":[383],"Stable":[384],"Isotope":[385],"Labeling":[386],"Amino":[388],"Acids":[389],"Cell":[391],"Culture":[392],"(SILAC)":[393],"quantitative":[395],"mass":[396],"were":[400,415],"increase":[403],"Fyn.":[412],"Several":[413],"selected":[416],"further":[418],"biochemically":[419],"validated.":[420],"data":[422],"suggest":[423],"allow":[430],"more":[434],"interact":[436],"with":[437],"discrete":[438],"pools":[439],"effectuate":[446],"signal":[448],"transduction.":[449],"Together":[450],"studies":[452],"provide":[453],"fundamental":[458],"processes":[459],"all":[462],"cells":[463],"function.":[464]},"counts_by_year":[],"updated_date":"2026-08-08T01:25:22.217667","created_date":"2016-06-24T00:00:00"}