{"id":"https://openalex.org/W2345306993","doi":"https://doi.org/10.1016/s0022-2275(20)38319-x","title":"A novel method for the analysis of platelet-activating factor: direct derivatization of glycerophospholipids.","display_name":"A novel method for the analysis of platelet-activating factor: direct derivatization of glycerophospholipids.","publication_year":1989,"publication_date":"1989-06-01","ids":{"openalex":"https://openalex.org/W2345306993","doi":"https://doi.org/10.1016/s0022-2275(20)38319-x","mag":"2345306993","pmid":"https://pubmed.ncbi.nlm.nih.gov/2794783"},"language":"en","primary_location":{"id":"doi:10.1016/s0022-2275(20)38319-x","doi":"https://doi.org/10.1016/s0022-2275(20)38319-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0022-2275(20)38319-x","pdf_url":null,"source":{"id":"https://openalex.org/S11400418","display_name":"Journal of Lipid Research","issn_l":"0022-2275","issn":["0022-2275","1539-7262"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Lipid Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0022-2275(20)38319-x","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086648107","display_name":"Rajiv K. Satsangi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R K Satsangi","raw_affiliation_strings":["Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109064644","display_name":"Janet C. Ludwig","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J C Ludwig","raw_affiliation_strings":["Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005466573","display_name":"Susan T. Weintraub","orcid":"https://orcid.org/0000-0002-8328-7814","observed_orcids":["https://orcid.org/0000-0002-8328-7814"]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S T Weintraub","raw_affiliation_strings":["Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750","institution_ids":["https://openalex.org/I165951966"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112431360","display_name":"R. Neal Pinckard","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165951966","display_name":"The University of Texas at San Antonio Health Science Center","ror":"https://ror.org/02f6dcw23","country_code":"US","type":"education","lineage":["https://openalex.org/I165951966","https://openalex.org/I45438204"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R N Pinckard","raw_affiliation_strings":["Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Texas Health Science Center, San Antonio 78284-7750","institution_ids":["https://openalex.org/I165951966"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I165951966"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":3.2889,"has_fulltext":false,"cited_by_count":20,"citation_normalized_percentile":{"value":0.92328699,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":null,"biblio":{"volume":"30","issue":"6","first_page":"929","last_page":"937"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.4884999990463257,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.4884999990463257,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.17960000038146973,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.12150000035762787,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/platelet-activating-factor","display_name":"platelet-activating factor","score":1.0},{"id":"https://openalex.org/keywords/glycerophospholipids","display_name":"glycerophospholipids","score":0.9549999833106995},{"id":"https://openalex.org/keywords/heptafluorobutyric-anhydride-derivatization","display_name":"heptafluorobutyric anhydride derivatization","score":0.9520000219345093},{"id":"https://openalex.org/keywords/positional-isomerization","display_name":"positional isomerization","score":0.7239999771118164},{"id":"https://openalex.org/keywords/molecular-species-identification","display_name":"DNA-based species identification","score":0.5879999995231628}],"concepts":[{"id":"https://openalex.org/C2909522894","wikidata":"https://www.wikidata.org/wiki/Q420004","display_name":"Glycerophospholipids","level":4,"score":0.9353245496749878},{"id":"https://openalex.org/C2776923573","wikidata":"https://www.wikidata.org/wiki/Q2294884","display_name":"Derivatization","level":3,"score":0.724116861820221},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5435949563980103},{"id":"https://openalex.org/C2779020240","wikidata":"https://www.wikidata.org/wiki/Q420577","display_name":"Platelet-activating factor","level":2,"score":0.4649205505847931},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3953026235103607},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.37483540177345276},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25352805852890015},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.15003490447998047},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.10383102297782898},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.06533968448638916},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D002621","descriptor_name":"Chemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005466","descriptor_name":"Fluorocarbons","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005994","descriptor_name":"Glycerophosphates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008401","descriptor_name":"Gas Chromatography-Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009682","descriptor_name":"Magnetic Resonance Spectroscopy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010742","descriptor_name":"Phospholipid Ethers","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010972","descriptor_name":"Platelet Activating Factor","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0022-2275(20)38319-x","doi":"https://doi.org/10.1016/s0022-2275(20)38319-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0022-2275(20)38319-x","pdf_url":null,"source":{"id":"https://openalex.org/S11400418","display_name":"Journal of Lipid Research","issn_l":"0022-2275","issn":["0022-2275","1539-7262"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Lipid Research","raw_type":"journal-article"},{"id":"pmid:2794783","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2794783","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of lipid research","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:98ce17b0e55640f2980e971a3352c872","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/98ce17b0e55640f2980e971a3352c872","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Lipid Research, Vol 30, Iss 6, Pp 929-937 (1989)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/s0022-2275(20)38319-x","doi":"https://doi.org/10.1016/s0022-2275(20)38319-x","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0022-2275(20)38319-x","pdf_url":null,"source":{"id":"https://openalex.org/S11400418","display_name":"Journal of Lipid Research","issn_l":"0022-2275","issn":["0022-2275","1539-7262"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Lipid Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4078640606","display_name":null,"funder_award_id":"AI-21818","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G4613112117","display_name":"MOLECULAR PATHOBIOLOGY OF PLATELET ACTIVATING FACTOR","funder_award_id":"R01HL022555","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W47799863","https://openalex.org/W276534841","https://openalex.org/W1503264380","https://openalex.org/W1539674135","https://openalex.org/W1567247348","https://openalex.org/W1595478289","https://openalex.org/W1826976144","https://openalex.org/W1987409651","https://openalex.org/W1993896238","https://openalex.org/W1996072942","https://openalex.org/W2000755900","https://openalex.org/W2001682079","https://openalex.org/W2005933871","https://openalex.org/W2017598713","https://openalex.org/W2023742203","https://openalex.org/W2041120355","https://openalex.org/W2042673584","https://openalex.org/W2047094765","https://openalex.org/W2058316046","https://openalex.org/W2069495150","https://openalex.org/W2076505666","https://openalex.org/W2152311269","https://openalex.org/W2159286650","https://openalex.org/W2171514292","https://openalex.org/W2233541602","https://openalex.org/W2261984195","https://openalex.org/W2335836844","https://openalex.org/W4233811497","https://openalex.org/W4298028619"],"related_works":["https://openalex.org/W2394577104","https://openalex.org/W2006104282","https://openalex.org/W1994303152","https://openalex.org/W937430935","https://openalex.org/W1955272010","https://openalex.org/W1973255382","https://openalex.org/W2010868010","https://openalex.org/W2047094765","https://openalex.org/W12010091","https://openalex.org/W2003713845"],"abstract_inverted_index":{"A":[0],"novel,":[1],"facile,":[2],"and":[3,9,17,74,85,173,190],"sensitive":[4],"method":[5,66,156],"for":[6,70,102,160],"the":[7,41,44,52,56,68,79,97,114,123,137,151,161,196],"quantitative":[8,191],"complete":[10,189],"structure-proof":[11],"analysis":[12],"of":[13,43,55,82,96,139,163,166,176,179,193,195,199],"platelet-activating":[14],"factor":[15],"(PAF)":[16],"other":[18],"glycerophospholipids":[19],"is":[20],"described.":[21],"1-O-Alkyl/acyl-2-acyl-3-glycerophospholipids":[22],"were":[23,48],"treated":[24],"with":[25,87,146],"heptafluorobutyric":[26],"anhydride":[27,89],"in":[28,93,127],"a":[29,128],"one-step":[30,129],"reaction":[31,58,130],"to":[32,122,136,149],"yield":[33],"1-O-alkyl/acyl-2-acyl-3-heptafluorobutyroyl-sn-glycerols":[34],"as":[35,60],"gas-liquid":[36,103],"chromatography":[37],"(GLC)-compatible":[38],"derivatives.":[39,62],"Furthermore,":[40],"components":[42],"polar":[45],"head":[46],"group":[47],"also":[49,110,143],"analyzed":[50],"from":[51],"aqueous":[53],"extract":[54],"same":[57],"mixture":[59],"t-butyldimethylsilyl":[61,125,132],"Thus,":[63],"this":[64,155,183],"new":[65],"eliminates":[67],"need":[69],"phospholipase":[71],"C":[72],"treatment":[73],"subsequent":[75],"purification":[76],"procedures.":[77],"Moreover,":[78],"direct":[80],"derivatization":[81,172],"PAF":[83,142,180],"homologs":[84],"analogs":[86],"hepatofluorobutyric":[88],"does":[90],"not":[91],"result":[92],"positional":[94],"isomerization":[95],"product,":[98],"providing":[99],"increased":[100],"specificity":[101],"chromatography-mass":[104],"spectrometric":[105],"(MS)":[106],"analysis.":[107],"It":[108],"has":[109,157],"been":[111],"shown":[112],"that":[113,182],"heptafluorobutyroyl":[115,140],"(HFB)":[116],"derivative":[117],"can":[118],"easily":[119],"be":[120],"converted":[121],"respective":[124],"analog":[126],"using":[131],"chloride/imidazole":[133],"reagent.":[134],"Analogous":[135],"formation":[138,162],"derivatives,":[141],"was":[144],"reacted":[145],"pentafluorobenzoyl":[147,152],"chloride":[148],"generate":[150],"derivative.":[153],"Therefore,":[154],"wide":[158],"applicability":[159],"GLC-compatible":[164],"derivatives":[165],"various":[167],"glycerophospholipids.":[168],"Our":[169],"successful":[170],"HFB":[171],"GLC-MS":[174],"detection":[175],"subnanogram":[177],"quantities":[178],"indicate":[181],"analytical":[184],"procedure":[185],"will":[186],"greatly":[187],"facilitate":[188],"identification":[192],"each":[194],"molecular":[197],"species":[198],"biologically":[200],"derived":[201],"PAF.":[202]},"counts_by_year":[],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}