{"id":"https://openalex.org/W2342294598","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","title":"Ellagic Acid and Quercetin are High\u2010Affinity Ligands of Human Peroxisome Proliferator\u2010Activated Receptor Alpha in an In\u2010Vitro Competitive Binding Assay","display_name":"Ellagic Acid and Quercetin are High\u2010Affinity Ligands of Human Peroxisome Proliferator\u2010Activated Receptor Alpha in an In\u2010Vitro Competitive Binding Assay","publication_year":2016,"publication_date":"2016-04-01","ids":{"openalex":"https://openalex.org/W2342294598","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","mag":"2342294598"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.30.1_supplement.691.7","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004385901","display_name":"Geoff Sasaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Geoff Sasaki","raw_affiliation_strings":["Food Science and Technology, Oregon State University, Corvallis, OR"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food Science and Technology, Oregon State University, Corvallis, OR","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001478855","display_name":"Ting F Luo","orcid":"https://orcid.org/0000-0002-3394-7861","observed_orcids":["https://orcid.org/0000-0002-3394-7861"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ting Luo","raw_affiliation_strings":["Food Science and Technology, Oregon State University, Corvallis, OR"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food Science and Technology, Oregon State University, Corvallis, OR","institution_ids":["https://openalex.org/I131249849"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034551982","display_name":"Neil F. Shay","orcid":"https://orcid.org/0000-0002-3021-1170","observed_orcids":["https://orcid.org/0000-0002-3021-1170"]},"institutions":[{"id":"https://openalex.org/I131249849","display_name":"Oregon State University","ror":"https://ror.org/00ysfqy60","country_code":"US","type":"education","lineage":["https://openalex.org/I131249849"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Neil F Shay","raw_affiliation_strings":["Food Science and Technology, Oregon State University, Corvallis, OR"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Food Science and Technology, Oregon State University, Corvallis, OR","institution_ids":["https://openalex.org/I131249849"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I131249849"],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.1731,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.608354,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"30","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11072","display_name":"Peroxisome Proliferator-Activated Receptors","score":0.9282000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11072","display_name":"Peroxisome Proliferator-Activated Receptors","score":0.9282000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12638","display_name":"Pomegranate: compositions and health benefits","score":0.029100000858306885,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10035","display_name":"Phytochemicals and Antioxidant Activities","score":0.006500000134110451,"subfield":{"id":"https://openalex.org/subfields/2704","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ellagic-acid","display_name":"ellagic acid","score":0.9610000252723694},{"id":"https://openalex.org/keywords/quercetin","display_name":"quercetin","score":1.0},{"id":"https://openalex.org/keywords/pparalpha","display_name":"PPAR\u03b1","score":0.9330000281333923},{"id":"https://openalex.org/keywords/phytochemicals","display_name":"phytochemicals","score":0.8849999904632568},{"id":"https://openalex.org/keywords/ligand-binding-affinity","display_name":"ligand binding affinity","score":0.8289999961853027},{"id":"https://openalex.org/keywords/non-alcoholic-fatty-liver-disease","display_name":"non-alcoholic fatty liver disease","score":0.8709999918937683},{"id":"https://openalex.org/keywords/daidzein","display_name":"daidzein","score":0.8069999814033508},{"id":"https://openalex.org/keywords/genistein","display_name":"genistein","score":1.0},{"id":"https://openalex.org/keywords/raspberry-ketone","display_name":"raspberry ketone","score":0.9980000257492065},{"id":"https://openalex.org/keywords/fenofibric-acid","display_name":"fenofibric acid","score":0.8389999866485596},{"id":"https://openalex.org/keywords/gw9662","display_name":"GW9662","score":0.9150000214576721},{"id":"https://openalex.org/keywords/nuclear-receptors","display_name":"nuclear receptors","score":0.7110000252723694},{"id":"https://openalex.org/keywords/competitive-binding-assay","display_name":"competitive binding assay","score":0.6340000033378601},{"id":"https://openalex.org/keywords/tr-fret","display_name":"time-resolved fluorescence resonance energy transfer","score":0.5529999732971191},{"id":"https://openalex.org/keywords/ic50","display_name":"IC50","score":0.47699999809265137}],"concepts":[{"id":"https://openalex.org/C2781464872","wikidata":"https://www.wikidata.org/wiki/Q422044","display_name":"Ellagic acid","level":4,"score":0.829073429107666},{"id":"https://openalex.org/C187345961","wikidata":"https://www.wikidata.org/wiki/Q419629","display_name":"Peroxisome proliferator-activated receptor","level":3,"score":0.5631083846092224},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5378180742263794},{"id":"https://openalex.org/C2781263971","wikidata":"https://www.wikidata.org/wiki/Q409478","display_name":"Quercetin","level":3,"score":0.5185118913650513},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4815295338630676},{"id":"https://openalex.org/C188413054","wikidata":"https://www.wikidata.org/wiki/Q18030677","display_name":"Peroxisome proliferator-activated receptor alpha","level":5,"score":0.47634533047676086},{"id":"https://openalex.org/C63932345","wikidata":"https://www.wikidata.org/wiki/Q422500","display_name":"Nuclear receptor","level":4,"score":0.452114462852478},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.4388962984085083},{"id":"https://openalex.org/C2776933373","wikidata":"https://www.wikidata.org/wiki/Q424744","display_name":"Fibrate","level":3,"score":0.42359378933906555},{"id":"https://openalex.org/C2778163477","wikidata":"https://www.wikidata.org/wiki/Q43656","display_name":"Cholesterol","level":2,"score":0.16206997632980347},{"id":"https://openalex.org/C2778004101","wikidata":"https://www.wikidata.org/wiki/Q133948","display_name":"Antioxidant","level":2,"score":0.1543494462966919},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.1297435760498047},{"id":"https://openalex.org/C70899900","wikidata":"https://www.wikidata.org/wiki/Q290439","display_name":"Polyphenol","level":3,"score":0.10835450887680054},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.30.1_supplement.691.7","doi":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.30.1_supplement.691.7","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.979200005531311}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4408649763","https://openalex.org/W4386704248","https://openalex.org/W4405840394","https://openalex.org/W2558996269","https://openalex.org/W3158110094","https://openalex.org/W2589776185","https://openalex.org/W3010741178","https://openalex.org/W4398185796","https://openalex.org/W4404054221","https://openalex.org/W2097293959"],"abstract_inverted_index":{"Introduction":[0],"Non\u2010Alcoholic":[1,28],"Fatty":[2],"Liver":[3],"Disease":[4],"(NAFLD)":[5],"is":[6,36,48,94,410,440],"a":[7,19,69,84,95,160,203,347,360,375],"condition":[8],"characterized":[9],"by":[10,173,469],"fat":[11],"accumulation":[12],"in":[13,103,307,317,356,416],"the":[14,66,87,125,144,167,175,196,231,238,259,312,340,363,381,392,407,413,417,426,433,441,451,462,472],"liver,":[15],"and":[16,31,45,107,140,143,153,181,186,224,235,248,272,284,296,316,389,391,420,455,486],"can":[17],"be":[18,372,397,403],"precursor":[20],"to":[21,50,53,64,74,166,258,297,309,335,374,386,396,402,443,450,464],"more":[22],"severe":[23],"liver":[24,32,106,427],"diseases,":[25],"such":[26,431],"as":[27,76,124,262,286,359,432],"Steatohepatitis,":[29],"cirrhosis":[30],"cancer.":[33],"Currently,":[34],"there":[35],"no":[37],"accepted":[38,218],"therapy":[39],"for":[40,43,68,78,193,292,324,394],"NAFLD,":[41],"except":[42],"diet":[44],"exercise,":[46],"which":[47],"difficult":[49],"administer":[51],"successfully":[52],"often":[54],"obese":[55],"individuals":[56],"diagnosed":[57],"with":[58,122,216,241,288,362,425],"NAFLD.":[59],"Objective":[60],"The":[61,130,199,252,279],"objective":[62],"was":[63,120,171,294],"measure":[65],"potential":[67,393,463],"set":[70],"of":[71,86,134,162,195,206,245,281,311,329,344,399,448,471],"select":[72],"phytochemicals":[73,145,254,331,449],"serve":[75],"ligands":[77],"human":[79,168,452],"peroxisome":[80],"proliferator\u2010activated":[81],"receptor\u2010alpha":[82],"(hPPAR\u03b1),":[83],"member":[85],"nuclear":[88],"hormone":[89],"receptor":[90,170,261],"(NHR)":[91],"superfamily.":[92],"PPAR\u03b1":[93,169,260,293,325,336,400,453,473],"key":[96],"regulator":[97],"factor":[98],"controlling":[99],"intracellular":[100],"lipid":[101],"oxidation":[102,468],"tissues":[104,430],"including":[105],"muscle.":[108,434],"Methods":[109],"A":[110],"time\u2010resolved":[111],"fluorescence":[112],"resonance":[113],"energy":[114],"transfer":[115],"(TR\u2010FRET)":[116],"competitive":[117],"binding":[118,127,207,304,447],"assay":[119],"used,":[121],"hPPAR\u03b1":[123],"ligand":[126,446],"domain":[128],"(ThermoFisher).":[129],"compounds":[131,201,287,459],"tested":[132,146,158,200],"consisted":[133],"two":[135],"positive":[136],"controls:":[137],"GW":[138],"9662":[139],"fenofibric":[141,338],"acid":[142,226,234,283,320,369,388,467],"were":[147,157,191,306],"daidzein,":[148],"ellagic":[149,233,282,319,368,387],"acid,":[150,339],"genistein,":[151],"quercetin,":[152,390],"raspberry":[154,273],"ketone.":[155],"Compounds":[156],"across":[159],"range":[161,205],"concentrations.":[163],"Binding":[164],"affinity":[165,291,323,334],"measured":[172],"detecting":[174],"fluorescent":[176],"emission":[177],"at":[178],"520":[179],"nm":[180],"495":[182],"nm;":[183],"competition":[184],"curves":[185],"an":[187],"IC":[188,212,242],"50":[189,213,243],"values":[190,214,244],"determined":[192],"each":[194],"compounds.":[197],"Results":[198],"demonstrated":[202],"wide":[204],"affinities.":[208],"Positive":[209],"controls":[210],"had":[211,321,332],"consistent":[215],"their":[217],"values:":[219],"GW9662":[220],"=":[221,265,269,275],"332":[222],"nM":[223,247],"Fenofibric":[225],"=@":[227],"48":[228],"\u03bcM.":[229],"For":[230],"phytochemicals,":[232],"quercetin":[236,285],"showed":[237],"greatest":[239],"affinities":[240,305],"269":[246],"1.3":[249],"\u03bcM,":[250,267],"respectively.":[251],"other":[253,408,429],"did":[255],"not":[256,300,371],"bind":[257],"well:":[263],"genistein":[264],"241":[266],"daidzein":[268],"4.3":[270],"M,":[271],"ketone":[274],"19":[276],"mM.":[277],"Conculsions":[278],"identification":[280],"relatively":[289],"high":[290],"unexpected,":[295],"our":[298,436],"knowledge,":[299,437],"previously":[301],"reported.":[302],"Their":[303],"close":[308],"that":[310],"high\u2010affinity":[313],"compound,":[314],"GW9662,":[315],"fact,":[318],"greater":[322],"than":[326,337],"GW9662.":[327],"Both":[328],"these":[330],"higher":[333],"pharmacologically":[341],"active":[342],"metabolite":[343],"fenofibrate.":[345],"Despite":[346],"lower":[348],"affinity,":[349],"this":[350,438],"compound":[351],"produces":[352],"significant":[353,376],"metabolic":[354],"effects":[355],"humans,":[357],"administered":[358],"drug":[361],"trade":[364],"name":[365],"Tricor.":[366],"Although":[367],"may":[370,460],"absorbed":[373,411],"degree,":[377],"its":[378],"downstream":[379],"metabolites,":[380],"urolithins,":[382],"have":[383,461],"similar":[384],"structure":[385],"urolithins":[395],"agonists":[398],"remains":[401],"tested.":[404],"Quercetin,":[405],"on":[406],"hand,":[409],"into":[412],"blood":[414],"stream":[415],"small":[418],"intestine":[419],"thus":[421],"could":[422],"interact":[423],"subsequently":[424],"and/or":[428],"To":[435],"work":[439],"first":[442],"demonstrate":[444],"direct":[445],"NHR":[454],"suggests":[456],"some":[457],"natural":[458],"enhance":[465],"fatty":[466],"transactivation":[470],"pathway.":[474],"Support":[475],"or":[476],"Funding":[477],"Information":[478],"Oregon":[479],"State":[480],"University":[481],"Undergraduate":[482],"Research,":[483],"Innovation,":[484],"Scholarship":[485],"Creativity":[487],"Award":[488],"Program":[489]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}