{"id":"https://openalex.org/W2341815592","doi":"https://doi.org/10.1158/1538-7445.am2015-1603","title":"Abstract 1603: High purity isolation of circulating tumor cells for next-generation sequencing","display_name":"Abstract 1603: High purity isolation of circulating tumor cells for next-generation sequencing","publication_year":2015,"publication_date":"2015-08-01","ids":{"openalex":"https://openalex.org/W2341815592","doi":"https://doi.org/10.1158/1538-7445.am2015-1603","mag":"2341815592"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2015-1603","doi":"https://doi.org/10.1158/1538-7445.am2015-1603","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2015-1603","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083095655","display_name":"Nur Lina Mohd Salleh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165932596","display_name":"National University of Singapore","ror":"https://ror.org/01tgyzw49","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Nur Lina Mohd Salleh","raw_affiliation_strings":["1National University of Singapore, Singapore, Singapore;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1National University of Singapore, Singapore, Singapore;","institution_ids":["https://openalex.org/I165932596"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054056110","display_name":"Yi Fang Lee","orcid":"https://orcid.org/0009-0005-4766-0123","observed_orcids":["https://orcid.org/0009-0005-4766-0123"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yi Fang Lee","raw_affiliation_strings":["2Clearbridge BioMedics, Singapore, Singapore;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Clearbridge BioMedics, Singapore, Singapore;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003521810","display_name":"Mei Hui Tan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mei Hui Tan","raw_affiliation_strings":["2Clearbridge BioMedics, Singapore, Singapore;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Clearbridge BioMedics, Singapore, Singapore;","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059469021","display_name":"Michelle A. Rosario","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103786","display_name":"National University Cancer Institute, Singapore","ror":"https://ror.org/025yypj46","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596","https://openalex.org/I4210103786","https://openalex.org/I4210150261"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Michelle A. Rosario","raw_affiliation_strings":["3National University Cancer Institute of Singapore, Singapore, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3National University Cancer Institute of Singapore, Singapore, Singapore","institution_ids":["https://openalex.org/I4210103786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032293215","display_name":"Ross Andrew Soo","orcid":"https://orcid.org/0000-0002-3053-3997","observed_orcids":["https://orcid.org/0000-0002-3053-3997"]},"institutions":[{"id":"https://openalex.org/I4210103786","display_name":"National University Cancer Institute, Singapore","ror":"https://ror.org/025yypj46","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596","https://openalex.org/I4210103786","https://openalex.org/I4210150261"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Ross A. Soo","raw_affiliation_strings":["3National University Cancer Institute of Singapore, Singapore, Singapore"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3National University Cancer Institute of Singapore, Singapore, Singapore","institution_ids":["https://openalex.org/I4210103786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086617305","display_name":"Ali Asgar S. Bhagat","orcid":"https://orcid.org/0000-0002-4695-8762","observed_orcids":["https://orcid.org/0000-0002-4695-8762"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ali Asgar Bhagat","raw_affiliation_strings":["2Clearbridge BioMedics, Singapore, Singapore;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Clearbridge BioMedics, Singapore, Singapore;","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113572531","display_name":"Richie Chuan Teck Soong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165932596","display_name":"National University of Singapore","ror":"https://ror.org/01tgyzw49","country_code":"SG","type":"education","lineage":["https://openalex.org/I165932596"]}],"countries":["SG"],"is_corresponding":false,"raw_author_name":"Richie Chuan Teck Soong","raw_affiliation_strings":["1National University of Singapore, Singapore, Singapore;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1National University of Singapore, Singapore, Singapore;","institution_ids":["https://openalex.org/I165932596"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"75","issue":"15_Supplement","first_page":"1603","last_page":"1603"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/circulating-tumor-cells","display_name":"circulating tumor cells","score":0.9990000128746033},{"id":"https://openalex.org/keywords/next-generation-sequencing","display_name":"next-generation sequencing","score":0.6060000061988831},{"id":"https://openalex.org/keywords/lung-adenocarcinoma","display_name":"lung adenocarcinoma","score":0.9110000133514404},{"id":"https://openalex.org/keywords/colorectal-cancer","display_name":"colorectal cancer","score":0.8980000019073486},{"id":"https://openalex.org/keywords/egfr-mutation","display_name":"EGFR mutation","score":0.7639999985694885},{"id":"https://openalex.org/keywords/kras-mutation","display_name":"KRAS mutation","score":0.9620000123977661},{"id":"https://openalex.org/keywords/metastasis","display_name":"metastasis","score":0.5400000214576721},{"id":"https://openalex.org/keywords/drug-resistance","display_name":"drug resistance","score":0.718999981880188},{"id":"https://openalex.org/keywords/liquid-biopsy","display_name":"liquid biopsy","score":0.6880000233650208},{"id":"https://openalex.org/keywords/nci-h1975","display_name":"NCI-H1975","score":0.734000027179718},{"id":"https://openalex.org/keywords/hct116-cells","display_name":"HCT116 cells","score":0.9990000128746033}],"concepts":[{"id":"https://openalex.org/C61238886","wikidata":"https://www.wikidata.org/wiki/Q5121689","display_name":"Circulating tumor cell","level":4,"score":0.7717052102088928},{"id":"https://openalex.org/C2781187634","wikidata":"https://www.wikidata.org/wiki/Q20969980","display_name":"KRAS","level":4,"score":0.7458858489990234},{"id":"https://openalex.org/C164928082","wikidata":"https://www.wikidata.org/wiki/Q6063435","display_name":"Ion semiconductor sequencing","level":4,"score":0.5623893737792969},{"id":"https://openalex.org/C2779529041","wikidata":"https://www.wikidata.org/wiki/Q22349364","display_name":"Liquid biopsy","level":3,"score":0.5399110317230225},{"id":"https://openalex.org/C526805850","wikidata":"https://www.wikidata.org/wiki/Q188874","display_name":"Colorectal cancer","level":3,"score":0.4846397042274475},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.43211278319358826},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4302121102809906},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42592889070510864},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4143785238265991},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4132165312767029},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.40228569507598877},{"id":"https://openalex.org/C2779013556","wikidata":"https://www.wikidata.org/wiki/Q181876","display_name":"Metastasis","level":3,"score":0.3897012174129486},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.31317809224128723},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.20823630690574646}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2015-1603","doi":"https://doi.org/10.1158/1538-7445.am2015-1603","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2015-1603","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9763000011444092,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2503401748","https://openalex.org/W4399319779","https://openalex.org/W4393084683","https://openalex.org/W2419638895","https://openalex.org/W4249234527","https://openalex.org/W2223376105","https://openalex.org/W2913522375","https://openalex.org/W1638268653","https://openalex.org/W2471107684","https://openalex.org/W2955611117"],"abstract_inverted_index":{"Abstract":[0],"Background:":[1],"Being":[2],"able":[3],"to":[4,198],"capture":[5],"circulating":[6,268],"tumor":[7,26,201,269],"cells":[8,108,202,270],"(CTCs)":[9],"and":[10,28,47,69,82,85,98,106,139,148,183,233,236],"characterize":[11],"them":[12],"at":[13],"a":[14,62,121,172],"molecular":[15],"level":[16],"promises":[17],"novel":[18],"insights":[19],"into":[20],"the":[21,59,125,142,231,278,283],"mechanisms":[22],"that":[23,157],"drive":[24],"metastasis,":[25],"recurrence":[27],"drug":[29],"resistance.":[30],"However,":[31],"next":[32],"generation":[33],"sequencing":[34],"(NGS)":[35],"of":[36,61,102,124,180,207,267,277,282],"CTCs":[37,117,158,208],"has":[38],"been":[39],"challenged":[40],"by":[41,135],"antibody-enrichment":[42],"bias,":[43],"low":[44,166,173],"CTC":[45,67,204,222],"purity":[46,223,265],"nucleic":[48],"acid":[49],"yields":[50],"commonly":[51],"derived":[52],"from":[53,209],"existing":[54],"technologies.":[55],"Here,":[56],"we":[57],"describe":[58],"results":[60],"new":[63,217],"label-free":[64,126,221],"protocol":[65,123,218],"for":[66,224,271,286],"enrichment":[68],"NGS":[70,189,225],"analysis.":[71,190],"Methods:":[72],"The":[73,216],"lung":[74],"adenocarcinoma":[75],"cell":[76,88],"line,":[77],"NCI-H1975,":[78],"with":[79,91,164],"EGFR":[80],"T790M":[81],"L858R":[83],"mutations,":[84],"colorectal":[86],"cancer":[87],"line":[89],"HCT-116,":[90],"KRAS":[92],"G13D":[93],"mutation,":[94],"were":[95,118,159,193],"fluorescently":[96],"labelled":[97],"spiked":[99,200],"in":[100,115,188,195,230],"concentrations":[101],"250,":[103],"50,":[104],"10":[105,199],"0":[107],"per":[109],"7.5":[110],"mL":[111],"healthy":[112],"volunteer":[113],"blood":[114],"duplicate.":[116],"enriched":[119,161],"using":[120,141],"modified":[122],"spiral":[127],"microfluidics-based":[128],"ClearCell\u00ae":[129],"FX":[130],"system.":[131,152],"DNA":[132,174],"was":[133,186],"extracted":[134],"QIAamp":[136],"Micro":[137],"kit":[138],"sequenced":[140],"Ion":[143,149],"AmpliSeq":[144],"Cancer":[145,287,297],"Hotspot":[146],"Panel":[147],"Torrent":[150],"PGM":[151],"Results:":[153],"Imaging":[154],"analysis":[155],"indicated":[156],"efficiently":[160],"(40-70%":[162],"recovery)":[163],"extremely":[165],"leukocyte":[167],"background":[168],"(650-1300":[169],"cells).":[170],"Despite":[171],"quantity":[175],"(<5ng),":[176],"an":[177],"average":[178],"depth":[179],"6897":[181],"reads":[182],"97%":[184],"coverage":[185],"achieved":[187],"Expected":[191],"mutations":[192],"detectable":[194],"samples":[196,211],"down":[197],"(0.5%":[203],"purity).":[205],"Analysis":[206],"clinical":[210,237],"is":[212],"currently":[213],"on-going.":[214],"Conclusion:":[215],"generated":[219],"sufficient":[220],"analysis,":[226],"overcoming":[227],"current":[228],"limitations":[229],"field,":[232],"facilitating":[234],"discovery":[235],"application.":[238],"Citation":[239],"Format:":[240],"Nur":[241],"Lina":[242],"Mohd":[243],"Salleh,":[244],"Yi":[245],"Fang":[246],"Lee,":[247],"Mei":[248],"Hui":[249],"Tan,":[250],"Michelle":[251],"A.":[252,255],"Rosario,":[253],"Ross":[254],"Soo,":[256],"Ali":[257],"Asgar":[258],"Bhagat,":[259],"Richie":[260],"Chuan":[261],"Teck":[262],"Soong.":[263],"High":[264],"isolation":[266],"next-generation":[272],"sequencing.":[273],"[abstract].":[274],"In:":[275],"Proceedings":[276],"106th":[279],"Annual":[280],"Meeting":[281],"American":[284],"Association":[285],"Research;":[288],"2015":[289],"Apr":[290],"18-22;":[291],"Philadelphia,":[292],"PA.":[293],"Philadelphia":[294],"(PA):":[295],"AACR;":[296],"Res":[298],"2015;75(15":[299],"Suppl):Abstract":[300],"nr":[301],"1603.":[302],"doi:10.1158/1538-7445.AM2015-1603":[303]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2016-06-24T00:00:00"}