{"id":"https://openalex.org/W2340325836","doi":null,"title":"Bedeutung der Expression von RAD51 f\u00fcr die Reparatur von DNA-Sch\u00e4den mittels Homologer Rekombination in humanen Tumorzelllinien","display_name":"Bedeutung der Expression von RAD51 f\u00fcr die Reparatur von DNA-Sch\u00e4den mittels Homologer Rekombination in humanen Tumorzelllinien","publication_year":2014,"publication_date":"2014-11-11","ids":{"openalex":"https://openalex.org/W2340325836","mag":"2340325836"},"language":"de","primary_location":{"id":"mag:2340325836","doi":null,"is_oa":false,"landing_page_url":"https://ediss.sub.uni-hamburg.de/volltexte/2014/7039/pdf/Dissertation.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5019859478","display_name":"Jasna Irena Seelbach","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jasna Irena Seelbach","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5019859478"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11914","display_name":"PARP inhibition in cancer therapy","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10769","display_name":"BRCA gene mutations in cancer","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rad51","display_name":"RAD51","score":0.9139999747276306},{"id":"https://openalex.org/keywords/double-strand-break-repair","display_name":"DNA double-strand break repair","score":0.9409999847412109},{"id":"https://openalex.org/keywords/breast-cancer-cell-lines","display_name":"breast cancer cell lines","score":0.9359999895095825},{"id":"https://openalex.org/keywords/s-phase-accumulation","display_name":"S phase accumulation","score":0.5019999742507935},{"id":"https://openalex.org/keywords/nuclear-transport","display_name":"nuclear transport","score":0.4189999997615814},{"id":"https://openalex.org/keywords/dna-repair-capacity","display_name":"DNA repair capacity","score":0.5249999761581421}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7266722917556763},{"id":"https://openalex.org/C2779012473","wikidata":"https://www.wikidata.org/wiki/Q3235015","display_name":"RAD51","level":4,"score":0.5295934081077576},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4523431360721588},{"id":"https://openalex.org/C29456083","wikidata":"https://www.wikidata.org/wiki/Q1221899","display_name":"Gynecology","level":1,"score":0.3304363787174225},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23067176342010498},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.17647817730903625},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.1673101782798767},{"id":"https://openalex.org/C143425029","wikidata":"https://www.wikidata.org/wiki/Q5205747","display_name":"DNA damage","level":3,"score":0.07647693157196045},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.05235940217971802}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2340325836","doi":null,"is_oa":false,"landing_page_url":"https://ediss.sub.uni-hamburg.de/volltexte/2014/7039/pdf/Dissertation.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9476000070571899,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":148,"referenced_works":["https://openalex.org/W1482231686","https://openalex.org/W1532833901","https://openalex.org/W1578840375","https://openalex.org/W1795529600","https://openalex.org/W1909122431","https://openalex.org/W1963993768","https://openalex.org/W1963997214","https://openalex.org/W1964417205","https://openalex.org/W1964742429","https://openalex.org/W1965301167","https://openalex.org/W1965531809","https://openalex.org/W1965607924","https://openalex.org/W1966479600","https://openalex.org/W1968099812","https://openalex.org/W1968376359","https://openalex.org/W1970578083","https://openalex.org/W1971290197","https://openalex.org/W1971577441","https://openalex.org/W1972098018","https://openalex.org/W1976385056","https://openalex.org/W1978054036","https://openalex.org/W1978872365","https://openalex.org/W1979385111","https://openalex.org/W1980034972","https://openalex.org/W1982635841","https://openalex.org/W1986172624","https://openalex.org/W1986328156","https://openalex.org/W1987345634","https://openalex.org/W1988749598","https://openalex.org/W1990026115","https://openalex.org/W1990734482","https://openalex.org/W1991591616","https://openalex.org/W1994224478","https://openalex.org/W1998452180","https://openalex.org/W1999554125","https://openalex.org/W2003685320","https://openalex.org/W2004125597","https://openalex.org/W2006299554","https://openalex.org/W2006364890","https://openalex.org/W2007138966","https://openalex.org/W2008073451","https://openalex.org/W2012126331","https://openalex.org/W2016156352","https://openalex.org/W2016208064","https://openalex.org/W2018884212","https://openalex.org/W2023353541","https://openalex.org/W2023356259","https://openalex.org/W2025047585","https://openalex.org/W2025404182","https://openalex.org/W2025647725","https://openalex.org/W2029304297","https://openalex.org/W2030093162","https://openalex.org/W2030912934","https://openalex.org/W2032234735","https://openalex.org/W2034100797","https://openalex.org/W2034409892","https://openalex.org/W2034629898","https://openalex.org/W2034702779","https://openalex.org/W2037695645","https://openalex.org/W2038875604","https://openalex.org/W2040323503","https://openalex.org/W2044292152","https://openalex.org/W2044910913","https://openalex.org/W2045506022","https://openalex.org/W2046082194","https://openalex.org/W2046388483","https://openalex.org/W2048839078","https://openalex.org/W2049844811","https://openalex.org/W2052090116","https://openalex.org/W2055727475","https://openalex.org/W2057132693","https://openalex.org/W2062226976","https://openalex.org/W2062284662","https://openalex.org/W2062739292","https://openalex.org/W2064965689","https://openalex.org/W2065022250","https://openalex.org/W2068087390","https://openalex.org/W2068727486","https://openalex.org/W2068769666","https://openalex.org/W2071137315","https://openalex.org/W2074699503","https://openalex.org/W2074956041","https://openalex.org/W2075930643","https://openalex.org/W2076716415","https://openalex.org/W2077436902","https://openalex.org/W2077892030","https://openalex.org/W2079659589","https://openalex.org/W2082771722","https://openalex.org/W2083843455","https://openalex.org/W2085544302","https://openalex.org/W2086449675","https://openalex.org/W2089159132","https://openalex.org/W2089191852","https://openalex.org/W2090550749","https://openalex.org/W2090564839","https://openalex.org/W2096380982","https://openalex.org/W2099607826","https://openalex.org/W2101657043","https://openalex.org/W2106130068","https://openalex.org/W2106515413","https://openalex.org/W2106671241","https://openalex.org/W2106952961","https://openalex.org/W2109465247","https://openalex.org/W2109879947","https://openalex.org/W2111420919","https://openalex.org/W2118446587","https://openalex.org/W2121469024","https://openalex.org/W2123825855","https://openalex.org/W2126404651","https://openalex.org/W2126668502","https://openalex.org/W2129499391","https://openalex.org/W2129588276","https://openalex.org/W2130730147","https://openalex.org/W2131646714","https://openalex.org/W2131978108","https://openalex.org/W2134071049","https://openalex.org/W2136367213","https://openalex.org/W2138494428","https://openalex.org/W2139034746","https://openalex.org/W2139380885","https://openalex.org/W2143875501","https://openalex.org/W2144550200","https://openalex.org/W2145092167","https://openalex.org/W2146204567","https://openalex.org/W2146533600","https://openalex.org/W2147774152","https://openalex.org/W2149795092","https://openalex.org/W2154417289","https://openalex.org/W2154421320","https://openalex.org/W2155177412","https://openalex.org/W2155480439","https://openalex.org/W2157663766","https://openalex.org/W2158546639","https://openalex.org/W2158699530","https://openalex.org/W2159729748","https://openalex.org/W2162412306","https://openalex.org/W2163062435","https://openalex.org/W2165385395","https://openalex.org/W2166826534","https://openalex.org/W2168104423","https://openalex.org/W2168844822","https://openalex.org/W2168964269","https://openalex.org/W2169385687","https://openalex.org/W2172177944","https://openalex.org/W2172247857","https://openalex.org/W2334990949","https://openalex.org/W2426396336","https://openalex.org/W2463553839"],"related_works":["https://openalex.org/W6889879514","https://openalex.org/W1743206134","https://openalex.org/W2362634502","https://openalex.org/W3030603267","https://openalex.org/W2049073338","https://openalex.org/W2034409892","https://openalex.org/W1795529600","https://openalex.org/W278036528","https://openalex.org/W211438262","https://openalex.org/W3169718874"],"abstract_inverted_index":{"Das":[0],"Ziel":[1],"dieser":[2,330],"Arbeit":[3,331],"war":[4,133,273],"es":[5],"zu":[6,23,263,281,340],"klaren,":[7],"warum":[8],"eine":[9,174,177,196,211,233,251,259,278,308,314,324,336,387,410,438],"erhohte":[10,315,337,392],"RAD51-Expression,":[11],"welches":[12,210],"das":[13,28],"Schlusselprotein":[14],"der":[15,30,52,59,79,103,208,243,261,285,299,356,363,369,384,395,413],"Homologen":[16],"Rekombination":[17],"(HR)":[18],"ist,":[19,216],"in":[20,51,78,94,182,207,228,329,383,412,424,476,500,530,545,619,647,650,684,758,812,849,865],"vielen":[21],"Tumorentitaten":[22,70],"einer":[24,83,130,141,150,248,282,321,341],"negativen":[25],"Prognose":[26,375,420,439],"fur":[27,68,213,376,417,437],"Uberleben":[29],"Tumorpatienten":[31,377],"fuhrt.":[32],"Fur":[33,156],"Brust":[34],"(BrCa)-":[35],"und":[36,57,148,164,390,600],"Kopf-Hals":[37],"(HNSCC)-Tumore":[38],"sollte":[39,423],"daher":[40],"anhand":[41],"von":[42,117,162,168,199,205,230,275,344,352],"jeweils":[43,73,136],"zehn":[44],"Zelllinien":[45,72,183,232,244],"untersucht":[46],"werden,":[47,66,295,334],"wie":[48],"sich":[49,188],"Unterschiede":[50],"RAD51-Expression":[53,80,132,143,152,176,186,223,310,338,364,380,430],"auf":[54,100,190,195,386],"die":[55,71,101,114,134,157,214,297,307,367,415,418,429,433],"Kapazitat":[56,102],"Funktionalitat":[58],"DSB-Reparatur":[60],"mittels":[61,125,347,358],"HR":[62,126,215,348,359,519,803],"auswirken.\r\nEs":[63],"konnte":[64,217,293,407],"gezeigt":[65,294,333],"dass":[67,296,335],"beide":[69],"ein":[74,109,160,166],"sehr":[75,84,87,138,154,234],"breites":[76],"Spektrum":[77],"aufwiesen,":[81,311],"mit":[82,120,184,265,320,378,403],"niedrigen":[85],"bis":[86],"hohen":[88,151],"Expression.":[89],"Die":[90,350],"unterschiedliche":[91],"Menge":[92],"hatte":[93],"beiden":[95],"Entitaten":[96],"einen":[97,191],"erheblichen":[98],"Einfluss":[99],"HR.":[104,552,791],"Zum":[105],"Nachweis":[106],"wurde":[107,159],"dabei":[108,257],"Reparaturplasmid":[110],"verwendet,":[111],"bei":[112,140,149,221],"dem":[113],"intakte":[115],"Reparatur":[116,343,351],"Doppelstrangbruchen":[118],"(DSBs)":[119],"zwei":[121],"offenen":[122,268],"Enden":[123],"(zwei-endig)":[124],"bestimmt":[127],"wurde.":[128],"Bei":[129,305,399],"geringen":[131],"HR-Kapazitat":[135,181,226,250,389],"nur":[137,173,266],"gering,":[139],"moderaten":[142],"dagegen":[144],"immer":[145,339],"am":[146],"hochsten":[147],"wiederum":[153],"gering.":[155],"BrCa-Zelllinien":[158],"Verhaltnis":[161,167],"0,4:1,0:0,3":[163,599],"HNSCC-Zelllinien":[165],"0,3:1,0:0,2":[169],"gefunden.":[170],"Somit":[171],"ermoglicht":[172],"moderate":[175,579,611],"maximale":[178],"HR-Kapazitat.\r\nDie":[179],"geringe":[180,225],"hoher":[185,222],"lies":[187],"weder":[189],"verminderten":[192,249,342],"Kerntransport":[193],"noch":[194],"reduzierte":[197],"Ausbildung":[198],"RAD51-Reparaturaggregaten":[200],"zuruckfuhren.":[201],"Eine":[202],"verminderte":[203,388],"Akkumulation":[204],"Zellen":[206,286,396],"S-Phase,":[209],"Voraussetzung":[212],"ebenfalls":[218],"ausgeschlossen":[219],"werden.\r\nDie":[220],"beobachtete":[224],"bewirkte":[227,312],"funf":[229],"sieben":[231],"hohe":[235,309],"zellulare":[236],"Empfindlichkeit":[237],"gegenuber":[238,253,287,326],"Mitomycin":[239,681],"C":[240,682],"(MMC).":[241],"Zwei":[242],"zeigten":[245],"jedoch":[246],"trotz":[247],"Resistenz":[252,272,325],"MMC.":[254],"MMC":[255,288,704],"fuhrt":[256],"uber":[258],"Storung":[260],"Replikation":[262],"DSBs":[264,346,354],"einem":[267],"Ende":[269],"(ein-endig).":[270],"Diese":[271],"abhangig":[274],"RAD51,":[276,456,722],"da":[277],"RAD51":[279,726],"Inhibition":[280],"starken":[283],"Sensitivierung":[284],"fuhrte.":[289,349],"In":[290,748,792],"weiteren":[291],"Versuchen":[292],"Aktivierung":[298,323],"Intra-S-Phase":[300],"Signalkaskade":[301,372,406],"hierfur":[302],"entscheidend":[303],"war.":[304],"Zelllinien,":[306],"damit":[313,391],"CHK1":[316],"(Checkpoint":[317],"Kinase":[318],"1)-Expression":[319],"intakten":[322],"MMC.\r\nZusammenfassend":[327],"kann":[328,381],"erstmalig":[332],"zwei-endigen":[345],"ein-endigen":[353],"an":[355,490,755,838],"Replikationsgabel":[357],"wird":[360],"aber":[361,408],"neben":[362],"auch":[365,409,432],"durch":[366],"Starke":[368],"ubergeordneten":[370],"CHK1-vermittelten":[371],"bestimmt.\r\nDie":[373],"negative":[374,467,820],"erhohter":[379,401],"somit":[382],"Regel":[385],"genomische":[393],"Instabilitat":[394],"zuruckgefuhrt":[397],"werden.":[398,441],"gleichzeitig":[400],"CHK1-Expression":[402,434],"entsprechend":[404],"ausgepragter":[405],"Zunahme":[411],"Therapieresistenz":[414],"Ursache":[416],"schlechte":[419],"sein.":[421],"Deshalb":[422],"zukunftigen":[425],"klinischen":[426],"Studien":[427],"sowohl":[428],"als":[431,435],"Biomarker":[436],"uberpruft":[440],"\nThe":[442],"aim":[443],"of":[444,455,459,473,479,504,507,513,528,551,555,598,606,640,645,687,725,737,787,796,806,842],"this":[445,771],"study":[446,523,772],"was":[447,463,520,569,626,663,719,741,810],"to":[448,539,629,633,678,721,730,760,766,814,833,851,861],"elucidate":[449],"why":[450],"a":[451,466,525,536,540,558,562,575,578,585,588,596,604,610,614,623,630,637,654,659,670,674,696,752,761,779,784,815,826,834,852,858],"highly":[452,547],"elevated":[453,756,839,847],"expression":[454],"key":[457],"player":[458],"homologous":[460],"recombination":[461],"(HR),":[462],"identified":[464],"as":[465,871,873,879],"prognostic":[468],"marker":[469],"for":[470,661,743,774,794,822,881],"the":[471,502,549,556,581,634,648,738,744,767,775,804,819,869,874],"survival":[472],"tumor":[474,532,823,843,862],"patients":[475,824],"several":[477],"kinds":[478],"tumor.":[480],"For":[481,553],"investigations":[482,554],"ten":[483,488],"breast":[484],"cancer":[485],"(BrCa)":[486],"and":[487,511,584,837],"head":[489],"neck":[491],"squamous":[492],"cell":[493,496,593,602,620,651,689,693,749],"carcinoma":[494],"(HNSCC)":[495],"lines":[497,594,603,621,652,694,750],"were":[498],"chosen,":[499],"which":[501,657],"effect":[503],"different":[505],"levels":[506],"RAD51-expression":[508,529,546,573,580,587,612,625,668,754,781,828,870],"on":[509],"capacity":[510,550],"functionality":[512],"double-strand":[514],"break":[515],"(dsb)":[516],"repair":[517,559,786,795],"via":[518,790,802],"analyzed.":[521],"\r\nThis":[522],"revealed":[524,574,673],"broad":[526],"spectra":[527],"each":[531],"entity,":[533],"ranging":[534],"from":[535],"very":[537,541],"low":[538,572,576,589,617,671],"high":[542,586,624,655,667,675,753,780,816,827],"RAD51-expression.":[543],"Differences":[544],"influenced":[548],"HR-capacity":[557,583,590,618,672,836],"plasmid":[560],"containing":[561],"dsb":[563],"with":[564,622,653,669,680,707,715,751,825],"two":[565,692],"open":[566,710],"ends":[567],"(two-ended)":[568],"used.":[570],"A":[571,642,666,845],"HR-capacity,":[577],"highest":[582],"again.":[591],"BrCa":[592],"displayed":[595],"ratio":[597,605],"HNSCC":[601],"0,3:1,0:0,2.":[607],"Therefore,":[608,864],"only":[609,708],"allowed":[613],"maximal":[615],"HR-capacity.\r\nThe":[616],"neither":[627],"due":[628,720],"diminished":[631,785,835],"transport":[632],"nucleus":[635],"nor":[636],"reduced":[638,643],"formation":[639],"RAD51-foci.":[641],"accumulation":[644],"cells":[646,729],"S-phase":[649],"RAD51-expression,":[656],"is":[658],"requirement":[660],"HR,":[662],"also":[664,856],"excluded.":[665],"cellular":[676,746],"sensitivity":[677],"treatment":[679],"(MMC)":[683],"five":[685],"out":[686],"seven":[688],"lines.":[690],"Although":[691],"expressed":[695],"great":[697],"resistance":[698,718,860],"against":[699],"MMC,":[700],"despite":[701],"their":[702],"low\r\nHR-capacity.":[703],"causes":[705],"dsbs":[706,789,798],"one":[709],"end":[711],"(one-ended)":[712],"by":[713],"interfering":[714],"replication.":[716],"This":[717],"because":[723],"inhibition":[724],"sensitized":[727],"those":[728],"MMC-treatment.":[731],"Further":[732],"experiments":[733],"showed":[734],"that":[735,778],"activation":[736,764],"intra-s-phase":[739],"cascade":[740],"crucial":[742,811],"mentioned":[745],"sensitivity.":[747],"CHK1-expression":[757,848,875],"addition":[759,813,850],"its":[762],"proper":[763],"contributed":[765],"observed":[768],"resistance.\r\nTaken":[769],"together,":[770],"shows":[773],"first":[776],"time":[777],"always":[782],"caused":[783],"two-ended":[788],"contrast,":[793],"one-ended":[797],"at":[799],"replication":[800],"forks":[801],"level":[805],"upstream":[807],"CHK1-faciliated":[808],"signaling":[809,854],"RAD51-expression.\r\nIn":[817],"conclusion,":[818],"prognosis":[821],"ordinarily":[829],"can":[830,855],"be":[831,877],"attributed":[832],"genomic":[840],"instability":[841],"cells.":[844],"simultaneously":[846],"functional":[853],"cause":[857],"higher":[859],"therapie.":[863],"future":[866],"clinical":[867],"trials":[868],"well":[872],"should":[876],"included":[878],"biomarkers":[880],"prognosis.":[882]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}