{"id":"https://openalex.org/W2328824636","doi":"https://doi.org/10.1021/bi500458t","title":"Enzyme Architecture: The Effect of Replacement and Deletion Mutations of Loop 6 on Catalysis by Triosephosphate Isomerase","display_name":"Enzyme Architecture: The Effect of Replacement and Deletion Mutations of Loop 6 on Catalysis by Triosephosphate Isomerase","publication_year":2014,"publication_date":"2014-05-13","ids":{"openalex":"https://openalex.org/W2328824636","doi":"https://doi.org/10.1021/bi500458t","mag":"2328824636","pmid":"https://pubmed.ncbi.nlm.nih.gov/24825099","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4051426"},"language":"en","primary_location":{"id":"doi:10.1021/bi500458t","doi":"https://doi.org/10.1021/bi500458t","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi500458t","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi500458t","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://pubs.acs.org/doi/pdf/10.1021/bi500458t","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5006496354","display_name":"Xiang Zhai","orcid":"https://orcid.org/0000-0003-0639-8710","observed_orcids":["https://orcid.org/0000-0003-0639-8710"]},"institutions":[{"id":"https://openalex.org/I63190737","display_name":"University at Buffalo, State University of New York","ror":"https://ror.org/01y64my43","country_code":"US","type":"education","lineage":["https://openalex.org/I63190737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiang Zhai","raw_affiliation_strings":["University at Buffalo , , , ,","Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University at Buffalo , , , ,","institution_ids":["https://openalex.org/I63190737"]},{"raw_affiliation_string":"Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States","institution_ids":["https://openalex.org/I63190737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075727812","display_name":"Maybelle Kho Go","orcid":"https://orcid.org/0000-0003-3894-2661","observed_orcids":["https://orcid.org/0000-0003-3894-2661"]},"institutions":[{"id":"https://openalex.org/I63190737","display_name":"University at Buffalo, State University of New York","ror":"https://ror.org/01y64my43","country_code":"US","type":"education","lineage":["https://openalex.org/I63190737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maybelle K. Go","raw_affiliation_strings":["University at Buffalo , , , ,","Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University at Buffalo , , , ,","institution_ids":["https://openalex.org/I63190737"]},{"raw_affiliation_string":"Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States","institution_ids":["https://openalex.org/I63190737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033972236","display_name":"AnnMarie C. O\u2019Donoghue","orcid":"https://orcid.org/0000-0001-8652-8225","observed_orcids":["https://orcid.org/0000-0001-8652-8225"]},"institutions":[{"id":"https://openalex.org/I190082696","display_name":"Durham University","ror":"https://ror.org/01v29qb04","country_code":"GB","type":"education","lineage":["https://openalex.org/I190082696"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"AnnMarie C. O\u2019Donoghue","raw_affiliation_strings":["Durham University , , ,","Department\rof Chemistry, Durham University, Durham DH1 3EL, U.K"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Durham University , , ,","institution_ids":["https://openalex.org/I190082696"]},{"raw_affiliation_string":"Department\rof Chemistry, Durham University, Durham DH1 3EL, U.K","institution_ids":["https://openalex.org/I190082696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062838517","display_name":"Tina L. Amyes","orcid":"https://orcid.org/0000-0001-5393-549X","observed_orcids":["https://orcid.org/0000-0001-5393-549X"]},"institutions":[{"id":"https://openalex.org/I63190737","display_name":"University at Buffalo, State University of New York","ror":"https://ror.org/01y64my43","country_code":"US","type":"education","lineage":["https://openalex.org/I63190737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tina L. Amyes","raw_affiliation_strings":["University at Buffalo , , , ,","Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University at Buffalo , , , ,","institution_ids":["https://openalex.org/I63190737"]},{"raw_affiliation_string":"Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States","institution_ids":["https://openalex.org/I63190737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047964465","display_name":"Scott D. Pegan","orcid":"https://orcid.org/0000-0002-2958-5319","observed_orcids":["https://orcid.org/0000-0002-2958-5319"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott D. Pegan","raw_affiliation_strings":["University of Georgia , , , ,","Department\rof Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, Georgia 30602, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Georgia , , , ,","institution_ids":["https://openalex.org/I165733156"]},{"raw_affiliation_string":"Department\rof Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, Georgia 30602, United States","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100741838","display_name":"Yan Wang","orcid":"https://orcid.org/0000-0003-3262-9930","observed_orcids":["https://orcid.org/0000-0003-3262-9930"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yan Wang","raw_affiliation_strings":["Yale University , , , ,","Department\rof Chemistry, Yale University, New Haven, Connecticut 06520, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yale University , , , ,","institution_ids":["https://openalex.org/I32971472"]},{"raw_affiliation_string":"Department\rof Chemistry, Yale University, New Haven, Connecticut 06520, United States","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076898136","display_name":"J. Patrick Loria","orcid":"https://orcid.org/0000-0002-4824-9089","observed_orcids":["https://orcid.org/0000-0002-4824-9089"]},"institutions":[{"id":"https://openalex.org/I32971472","display_name":"Yale University","ror":"https://ror.org/03v76x132","country_code":"US","type":"education","lineage":["https://openalex.org/I32971472"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Patrick Loria","raw_affiliation_strings":["Yale University , , , ,","Department\rof Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Yale University , , , ,","institution_ids":["https://openalex.org/I32971472"]},{"raw_affiliation_string":"Department\rof Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, United States","institution_ids":["https://openalex.org/I32971472"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010165126","display_name":"Andrew D. Mesecar","orcid":"https://orcid.org/0000-0002-1241-2577","observed_orcids":["https://orcid.org/0000-0002-1241-2577"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew D. Mesecar","raw_affiliation_strings":["Purdue University , , , ,","Departments\rof Biological Sciences and Chemistry, Purdue University, West Lafayette, Indiana 47907, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Purdue University , , , ,","institution_ids":["https://openalex.org/I219193219"]},{"raw_affiliation_string":"Departments\rof Biological Sciences and Chemistry, Purdue University, West Lafayette, Indiana 47907, United States","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5066402611","display_name":"John P. Richard","orcid":"https://orcid.org/0000-0002-0440-2387","observed_orcids":["https://orcid.org/0000-0002-0440-2387"]},"institutions":[{"id":"https://openalex.org/I63190737","display_name":"University at Buffalo, State University of New York","ror":"https://ror.org/01y64my43","country_code":"US","type":"education","lineage":["https://openalex.org/I63190737"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John P. Richard","raw_affiliation_strings":["University at Buffalo , , , ,","Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University at Buffalo , , , ,","institution_ids":["https://openalex.org/I63190737"]},{"raw_affiliation_string":"Department\rof Chemistry, University at Buffalo, Buffalo, New York 14221, United States","institution_ids":["https://openalex.org/I63190737"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5066402611"],"corresponding_institution_ids":["https://openalex.org/I63190737"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":{"value":4500,"currency":"USD","value_usd":4500},"fwci":1.5112,"has_fulltext":true,"cited_by_count":27,"citation_normalized_percentile":{"value":0.82304062,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"53","issue":"21","first_page":"3486","last_page":"3501"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.45680001378059387,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.45680001378059387,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.34689998626708984,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.11150000244379044,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/triosephosphate-isomerase","display_name":"triosephosphate isomerase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/chicken-muscle","display_name":"chicken muscle","score":0.5889999866485596},{"id":"https://openalex.org/keywords/substrate-binding","display_name":"substrate binding","score":0.4009999930858612},{"id":"https://openalex.org/keywords/electrostatic-interactions","display_name":"electrostatic interactions","score":0.6650000214576721},{"id":"https://openalex.org/keywords/stereoselectivity","display_name":"stereoselectivity","score":0.593999981880188},{"id":"https://openalex.org/keywords/deuterium-isotope-effect","display_name":"deuterium isotope effect","score":0.6119999885559082},{"id":"https://openalex.org/keywords/enzyme-catalysis","display_name":"enzyme catalysis","score":0.527999997138977}],"concepts":[{"id":"https://openalex.org/C2778727959","wikidata":"https://www.wikidata.org/wiki/Q24777683","display_name":"Triosephosphate isomerase","level":3,"score":0.9161190986633301},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.5996602773666382},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5817707180976868},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5740252137184143},{"id":"https://openalex.org/C2781264208","wikidata":"https://www.wikidata.org/wiki/Q118026","display_name":"Isomerase","level":3,"score":0.5263917446136475},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5209973454475403},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.5093942284584045},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.48104360699653625},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.4766063094139099},{"id":"https://openalex.org/C126661725","wikidata":"https://www.wikidata.org/wiki/Q903420","display_name":"Isomerization","level":3,"score":0.4664943218231201},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4265987277030945},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.2799822688102722},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2546754777431488},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004099","descriptor_name":"Dihydroxyacetone Phosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005986","descriptor_name":"Glyceraldehyde 3-Phosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005987","descriptor_name":"Glyceraldehyde-3-Phosphate Dehydrogenases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D005987","descriptor_name":"Glyceraldehyde-3-Phosphate Dehydrogenases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005987","descriptor_name":"Glyceraldehyde-3-Phosphate Dehydrogenases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005994","descriptor_name":"Glycerophosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007536","descriptor_name":"Isomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011817","descriptor_name":"Rabbits","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017384","descriptor_name":"Sequence Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019843","descriptor_name":"Archaeal Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019843","descriptor_name":"Archaeal Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1021/bi500458t","doi":"https://doi.org/10.1021/bi500458t","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi500458t","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi500458t","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3388574","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4051426","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4051426","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4051426","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Text"},{"id":"pmid:24825099","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24825099","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:dro.dur.ac.uk.OAI2:17565","doi":null,"is_oa":false,"landing_page_url":"http://dro.dur.ac.uk/17565/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400188","display_name":"Durham Research Online (Durham University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I190082696","host_organization_name":"Durham University","host_organization_lineage":["https://openalex.org/I190082696"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Biochemistry, 2014, Vol.53(21), pp.3486-3501 [Peer Reviewed Journal]","raw_type":"Article"},{"id":"pmh:oai:https://durham-repository.worktribe.com/:1397044","doi":null,"is_oa":true,"landing_page_url":"https://durham-repository.worktribe.com/output/1397044","pdf_url":"https://durham-repository.worktribe.com/file/1397044/1/Accepted%20Journal%20Article","source":{"id":"https://openalex.org/S4306400188","display_name":"Durham Research Online (Durham University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I190082696","host_organization_name":"Durham University","host_organization_lineage":["https://openalex.org/I190082696"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1021/bi500458t","doi":"https://doi.org/10.1021/bi500458t","is_oa":true,"landing_page_url":"https://doi.org/10.1021/bi500458t","pdf_url":"https://pubs.acs.org/doi/pdf/10.1021/bi500458t","source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3221894908","display_name":null,"funder_award_id":"GM39754","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G593937027","display_name":null,"funder_award_id":"MCB1121372","funder_id":"https://openalex.org/F4320306080","funder_display_name":"Foundation for the National Institutes of Health"},{"id":"https://openalex.org/G9000001099","display_name":null,"funder_award_id":"GM 39754","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306080","display_name":"Foundation for the National Institutes of Health","ror":"https://ror.org/00k86s890"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320338284","display_name":"Argonne National Laboratory","ror":"https://ror.org/05gvnxz63"}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2328824636.pdf"},"referenced_works_count":81,"referenced_works":["https://openalex.org/W49665239","https://openalex.org/W177751858","https://openalex.org/W1536886974","https://openalex.org/W1539796472","https://openalex.org/W1699752915","https://openalex.org/W1964033631","https://openalex.org/W1971026909","https://openalex.org/W1971942119","https://openalex.org/W1975460133","https://openalex.org/W1980568487","https://openalex.org/W1981428746","https://openalex.org/W1982260438","https://openalex.org/W1983753605","https://openalex.org/W1984113719","https://openalex.org/W1985066480","https://openalex.org/W1985856261","https://openalex.org/W1989052605","https://openalex.org/W1991984236","https://openalex.org/W1992270716","https://openalex.org/W1994376003","https://openalex.org/W1998507745","https://openalex.org/W2001641653","https://openalex.org/W2002570640","https://openalex.org/W2003960201","https://openalex.org/W2011061531","https://openalex.org/W2012297445","https://openalex.org/W2013198516","https://openalex.org/W2014715093","https://openalex.org/W2019956959","https://openalex.org/W2021678208","https://openalex.org/W2022496873","https://openalex.org/W2022935785","https://openalex.org/W2023688586","https://openalex.org/W2023882547","https://openalex.org/W2029929841","https://openalex.org/W2030284680","https://openalex.org/W2031965983","https://openalex.org/W2034889157","https://openalex.org/W2037612112","https://openalex.org/W2041372545","https://openalex.org/W2041768035","https://openalex.org/W2041849641","https://openalex.org/W2042056317","https://openalex.org/W2046696671","https://openalex.org/W2047546483","https://openalex.org/W2049207661","https://openalex.org/W2051095934","https://openalex.org/W2055594181","https://openalex.org/W2059917692","https://openalex.org/W2062107561","https://openalex.org/W2063444222","https://openalex.org/W2063746676","https://openalex.org/W2068885696","https://openalex.org/W2068971591","https://openalex.org/W2070427206","https://openalex.org/W2070615094","https://openalex.org/W2072302548","https://openalex.org/W2079049996","https://openalex.org/W2079594724","https://openalex.org/W2084462176","https://openalex.org/W2085162051","https://openalex.org/W2086494622","https://openalex.org/W2091639850","https://openalex.org/W2093308340","https://openalex.org/W2095098723","https://openalex.org/W2118129083","https://openalex.org/W2121704271","https://openalex.org/W2123751740","https://openalex.org/W2134024192","https://openalex.org/W2138090603","https://openalex.org/W2143991598","https://openalex.org/W2144050876","https://openalex.org/W2146298489","https://openalex.org/W2161262895","https://openalex.org/W2164689526","https://openalex.org/W2167922245","https://openalex.org/W2169821755","https://openalex.org/W2313854513","https://openalex.org/W2329265014","https://openalex.org/W2337672766","https://openalex.org/W4244736178"],"related_works":["https://openalex.org/W1964704650","https://openalex.org/W2003960201","https://openalex.org/W1971026909","https://openalex.org/W2278176906","https://openalex.org/W2143991598","https://openalex.org/W1981266618","https://openalex.org/W1982260438","https://openalex.org/W7007836264","https://openalex.org/W7096001732","https://openalex.org/W2063444222"],"abstract_inverted_index":{"Two":[0],"mutations":[1,304],"of":[2,24,50,72,79,83,117,132,140,147,177,180,203,206,227,243,259,271,280,295,308,332,343,351],"the":[3,16,38,46,63,73,80,99,115,129,133,148,151,155,160,181,185,201,214,221,228,240,253,286,292,306,316,326,337,341,352],"phosphodianion":[4],"gripper":[5],"loop":[6,17,39,108,145],"in":[7,44,89,102,107,122,167,173,252,266,273,330,340],"chicken":[8],"muscle":[9],"triosephosphate":[10],"isomerase":[11],"(cTIM)":[12],"were":[13],"examined:":[14],"(1)":[15],"deletion":[18],"mutant":[19,42,287],"(LDM)":[20],"formed":[21,265],"by":[22,62,159,187,194],"removal":[23],"residues":[25],"170-173":[26],"[Pompliano,":[27],"D.":[28],"L.,":[29],"et":[30],"al.":[31],"(1990)":[32],"Biochemistry":[33],"29,":[34],"3186-3194]":[35],"and":[36,110,113,164,169,189,213,245,262,268,282,334],"(2)":[37],"6":[40,109,146],"replacement":[41],"(L6RM),":[43],"which":[45],"N-terminal":[47],"hinge":[48],"sequence":[49,64],"TIM":[51,212],"from":[52,65,85,311],"eukaryotes,":[53],"166-PXW-168":[54],"(X":[55],"=":[56],"L":[57],"or":[58],"V),":[59],"is":[60,192,232],"replaced":[61],"archaea,":[66],"166-PPE-168.":[67],"The":[68,162,275],"X-ray":[69,207],"crystal":[70,208],"structure":[71],"L6RM":[74,100,134,149,165,222],"shows":[75],"a":[76,204,224,309,348],"large":[77,225],"displacement":[78],"side":[81,241],"chain":[82],"E168":[84,244],"that":[86,98,114,135,216,247,302],"for":[87,126,175,210,285,298,325],"W168":[88],"wild-type":[90,141,211,299],"cTIM.":[91,142],"Solution":[92],"nuclear":[93],"magnetic":[94],"resonance":[95],"data":[96],"show":[97],"results":[101,121],"significant":[103],"chemical":[104,123],"shift":[105,124],"changes":[106,125],"surrounding":[111],"regions,":[112],"binding":[116,230],"glycerol":[118],"3-phosphate":[119],"(G3P)":[120],"nuclei":[127],"at":[128,315],"active":[130],"site":[131],"are":[136,248,264,323],"smaller":[137,276],"than":[138],"those":[139],"Interactions":[143],"with":[144,291],"stabilize":[150],"enediolate":[152,318],"intermediate":[153],"toward":[154],"elimination":[156],"reaction":[157,339],"catalyzed":[158],"LDM.":[161],"LDM":[163],"result":[166],"800000-":[168],"23000-fold":[170],"decreases,":[171],"respectively,":[172],"kcat/Km":[174],"isomerization":[176,328,353],"GAP.":[178],"Saturation":[179],"LDM,":[182],"but":[183,336],"not":[184],"L6RM,":[186,215],"substrate":[188],"inhibitor":[190],"phosphoglycolate":[191],"detected":[193,324],"steady-state":[195],"kinetic":[196],"analyses.":[197],"We":[198],"propose,":[199],"on":[200],"basis":[202],"comparison":[205],"structures":[209],"ligands":[217],"bind":[218],"weakly":[219],"to":[220,234,250,313],"because":[223],"fraction":[226],"ligand":[229],"energy":[231],"utilized":[233],"overcome":[235],"destabilizing":[236],"electrostatic":[237],"interactions":[238],"between":[239],"chains":[242],"E129":[246],"predicted":[249],"develop":[251],"loop-closed":[254],"enzyme.":[255],"Similar":[256],"normalized":[257,277],"yields":[258],"DHAP,":[260],"d-DHAP,":[261],"d-GAP":[263],"LDM-":[267],"L6RM-catalyzed":[269,338],"reactions":[270,289,329],"GAP":[272],"D2O.":[274],"12-13%":[278],"yield":[279,294,350],"DHAP":[281],"d-DHAP":[283],"observed":[284],"cTIM-catalyzed":[288],"compared":[290],"79%":[293],"these":[296,303],"products":[297,322],"cTIM":[300],"suggests":[301],"impair":[305],"transfer":[307],"proton":[310],"O-2":[312],"O-1":[314],"initial":[317],"phosphate":[319],"intermediate.":[320],"No":[321],"LDM-catalyzed":[327],"D2O":[331],"[1-(13)C]GA":[333],"HPi,":[335],"presence":[342],"0.020":[344],"M":[345],"dianion":[346],"gives":[347],"2%":[349],"product":[354],"[2-(13)C,2-(2)H]GA.":[355]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}