{"id":"https://openalex.org/W2328029902","doi":null,"title":"Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.","display_name":"Maintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.","publication_year":1993,"publication_date":"1993-09-01","ids":{"openalex":"https://openalex.org/W2328029902","mag":"2328029902","pmid":"https://pubmed.ncbi.nlm.nih.gov/7689481"},"language":"en","primary_location":{"id":"pmid:7689481","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7689481","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056104532","display_name":"Peter M. Lansdorp","orcid":"https://orcid.org/0000-0001-7435-1071","observed_orcids":["https://orcid.org/0000-0001-7435-1071"]},"institutions":[{"id":"https://openalex.org/I1289530486","display_name":"BC Cancer Agency","ror":"https://ror.org/03sfybe47","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I1289530486"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P M Lansdorp","raw_affiliation_strings":["Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada","institution_ids":["https://openalex.org/I1289530486"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023509010","display_name":"Wieslawa H. Dragowska","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W Dragowska","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":5.0207,"has_fulltext":false,"cited_by_count":61,"citation_normalized_percentile":{"value":0.95776974,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"21","issue":"10","first_page":"1321","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoiesis","display_name":"hematopoiesis","score":0.8629999756813049},{"id":"https://openalex.org/keywords/bone-marrow-derived-progenitor-cells","display_name":"bone marrow progenitor cells","score":0.7519999742507935},{"id":"https://openalex.org/keywords/serum-free-suspension-culture","display_name":"serum-free suspension culture","score":0.675000011920929},{"id":"https://openalex.org/keywords/interleukin-6","display_name":"interleukin-6","score":0.8669999837875366},{"id":"https://openalex.org/keywords/interleukin-3","display_name":"interleukin-3","score":0.9869999885559082},{"id":"https://openalex.org/keywords/stem-cell-factor","display_name":"stem cell factor","score":0.8389999866485596},{"id":"https://openalex.org/keywords/erythropoietin","display_name":"erythropoietin","score":0.9850000143051147},{"id":"https://openalex.org/keywords/self-renewal","display_name":"self-renewal","score":0.7459999918937683},{"id":"https://openalex.org/keywords/cell-turnover","display_name":"cell turnover","score":0.46700000762939453},{"id":"https://openalex.org/keywords/clonal-heterogeneity","display_name":"clonal heterogeneity","score":0.8080000281333923},{"id":"https://openalex.org/keywords/cell-cycle-kinetics","display_name":"cell cycle kinetics","score":0.7170000076293945},{"id":"https://openalex.org/keywords/pkh26-labeling","display_name":"PKH26 labeling","score":0.7770000100135803}],"concepts":[{"id":"https://openalex.org/C10205521","wikidata":"https://www.wikidata.org/wiki/Q4035578","display_name":"CD34","level":3,"score":0.8199965357780457},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.7336794137954712},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6318511962890625},{"id":"https://openalex.org/C2022786","wikidata":"https://www.wikidata.org/wiki/Q909331","display_name":"Stem cell factor","level":4,"score":0.621345043182373},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.608027994632721},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5544586777687073},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.5440589189529419},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4952501356601715},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4514646530151367},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4285932779312134},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42596128582954407},{"id":"https://openalex.org/C131665280","wikidata":"https://www.wikidata.org/wiki/Q14866169","display_name":"Interleukin 3","level":5,"score":0.4134080111980438},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4130861163139343},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4105381965637207},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.3840220272541046},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.11794629693031311},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11711132526397705},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10883057117462158},{"id":"https://openalex.org/C129374314","wikidata":"https://www.wikidata.org/wiki/Q909381","display_name":"Interleukin 21","level":4,"score":0.0},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000944","descriptor_name":"Antigens, Differentiation, B-Lymphocyte","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001769","descriptor_name":"Blood","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001854","descriptor_name":"Bone Marrow Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002999","descriptor_name":"Clone Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D004921","descriptor_name":"Erythropoietin","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006410","descriptor_name":"Hematopoiesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007377","descriptor_name":"Interleukin-3","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011990","descriptor_name":"Receptors, Transferrin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015850","descriptor_name":"Interleukin-6","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D016298","descriptor_name":"Hematopoietic Cell Growth Factors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D017493","descriptor_name":"Leukocyte Common Antigens","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D018952","descriptor_name":"Antigens, CD34","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019089","descriptor_name":"Stem Cell Factor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:7689481","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7689481","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Experimental hematology","raw_type":"Journal Article"},{"id":"mag:2328029902","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7689481","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2056604536","https://openalex.org/W1989163340","https://openalex.org/W7096554763","https://openalex.org/W1995440539","https://openalex.org/W2468247134","https://openalex.org/W2266949108","https://openalex.org/W2162833666","https://openalex.org/W2911438932","https://openalex.org/W2401355733","https://openalex.org/W1524481042"],"abstract_inverted_index":{"We":[0],"previously":[1],"described":[2],"that":[3,191,268],"cells":[4,70,112,131,149,158,180,190,214,227,239,275,313],"with":[5,24,115,240,246],"a":[6,170,233,241,247,279,284],"CD34+CD71lo":[7,69,128,238],"phenotype":[8],"from":[9,166,182,204],"adult":[10],"human":[11],"bone":[12,272],"marrow":[13],"are":[14],"maintained":[15],"at":[16,136,200,306],"constant":[17],"numbers":[18,46],"in":[19,44,47,71,133,173,216,228,257,299],"long-term":[20],"suspension":[21],"cultures":[22,49,73,135,230],"supplemented":[23],"interleukin-6":[25],"(IL-6),":[26],"IL-3,":[27,288],"mast":[28],"growth":[29],"factor":[30],"(MGF)":[31],"(a":[32],"c-kit":[33],"ligand),":[34],"and":[35,91,129,156,208,263,266,290],"erythropoietin":[36],"(Epo).":[37],"In":[38,107,143],"view":[39],"of":[40,68,78,86,94,124,127,177,189,212,224,235,237,251,271,286,302,310],"the":[41,65,95,108,116,134,144,175,187,195,210,221,269,300],"large":[42],"increase":[43],"cell":[45,197,258],"such":[48],"(for":[50],"example,":[51],">":[52],"10(6)-fold":[53],"per":[54],"cell),":[55],"this":[56],"was":[57],"an":[58],"unexpected":[59],"finding.":[60],"The":[61],"following":[62],"models":[63],"for":[64],"observed":[66,222],"maintenance":[67,223],"our":[72,229],"were":[74,100,113,150,159],"considered:":[75],"(1)":[76,174],"survival":[77,236],"non-dividing":[79],"cells;":[80,87],"(2)":[81,186],"self-renewal":[82],"balanced":[83],"by":[84,122,162],"loss":[85],"(3)":[88,209],"asymmetrical":[89],"divisions;":[90],"(4)":[92],"combinations":[93],"above.":[96],"Two":[97],"experimental":[98,303],"strategies":[99,304],"explored":[101],"to":[102,140,184,194,206,283,294],"discriminate":[103],"between":[104,261],"these":[105,167],"models.":[106],"first,":[109],"sorted":[110,152],"CD34+CD45RAloCD71lo":[111,148,274],"labeled":[114],"flourescent":[117],"tracking":[118],"dye":[119],"PKH26,":[120],"followed":[121],"analysis":[123],"PKH26":[125],"fluorescence":[126],"other":[130],"present":[132,215],"various":[137],"times":[138],"(up":[139],"11":[141],"weeks).":[142],"second":[145],"approach,":[146],"single":[147],"directly":[151],"into":[153,297],"individual":[154,217],"wells,":[155],"growing":[157],"then":[160],"analyzed":[161],"flow":[163],"cytometry.":[164],"Results":[165],"experiments":[168],"indicated":[169],"considerable":[171],"variability":[172],"number":[176,211],"surviving":[178],"input":[179],"(ranging":[181,203],"30":[183],"80%);":[185],"proportion":[188],"contributed":[192],"significantly":[193],"total":[196],"production":[198,250],"measured":[199],"day":[201],"20":[202],"1":[205],"5%);":[207],"CD34+":[213,226,252,262],"clones.":[218],"Taken":[219],"together,":[220],"primitive":[225,311],"apparently":[231],"involves":[232],"combination":[234],"vary":[242],"low":[243],"turnover":[244],"together":[245],"very":[248],"limited":[249],"cells.":[253],"Clonal":[254],"heterogeneity,":[255],"differences":[256],"cycle":[259],"kinetics":[260],"CD34-":[264],"cells,":[265],"observations":[267],"majority":[270],"marrow-derived":[273],"do":[276],"not":[277],"show":[278],"rapid":[280],"proliferative":[281],"response":[282],"mixture":[285],"IL-6,":[287],"MGF,":[289],"Epo":[291],"will":[292],"have":[293],"be":[295],"taken":[296],"account":[298],"development":[301],"aimed":[305],"clinically":[307],"useful":[308],"expansion":[309],"hematopoietic":[312],"ex":[314],"vivo.":[315]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}