{"id":"https://openalex.org/W2319568809","doi":"https://doi.org/10.1021/sb4001432","title":"Optimization of a Yeast RNA Interference System for Controlling Gene Expression and Enabling Rapid Metabolic Engineering","display_name":"Optimization of a Yeast RNA Interference System for Controlling Gene Expression and Enabling Rapid Metabolic Engineering","publication_year":2013,"publication_date":"2013-12-11","ids":{"openalex":"https://openalex.org/W2319568809","doi":"https://doi.org/10.1021/sb4001432","mag":"2319568809","pmid":"https://pubmed.ncbi.nlm.nih.gov/24328131"},"language":"en","primary_location":{"id":"doi:10.1021/sb4001432","doi":"https://doi.org/10.1021/sb4001432","is_oa":false,"landing_page_url":"https://doi.org/10.1021/sb4001432","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058374954","display_name":"Nathan Crook","orcid":"https://orcid.org/0000-0001-6165-1972","observed_orcids":["https://orcid.org/0000-0001-6165-1972"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nathan C. Crook","raw_affiliation_strings":["Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Alexander C. Schmitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander C. Schmitz","raw_affiliation_strings":["Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023917549","display_name":"Hal S. Alper","orcid":"https://orcid.org/0000-0002-8246-8605","observed_orcids":["https://orcid.org/0000-0002-8246-8605"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hal S. Alper","raw_affiliation_strings":["Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States","Institute for\rCellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway\rAvenue, Austin, Texas 78712, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department\rof Chemical Engineering, The University of Texas at Austin, 200\rEast Dean Keeton Street Stop C0400, Austin, Texas 78712, United States","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Institute for\rCellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway\rAvenue, Austin, Texas 78712, United States","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5023917549"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":3.0351,"has_fulltext":false,"cited_by_count":79,"citation_normalized_percentile":{"value":0.93244422,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"3","issue":"5","first_page":"307","last_page":"313"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9771000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.019500000402331352,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.9549999833106995},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.953000009059906},{"id":"https://openalex.org/keywords/gene-knockdown","display_name":"gene knockdown","score":0.9430000185966492},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.859000027179718},{"id":"https://openalex.org/keywords/itaconic-acid-production","display_name":"itaconic acid production","score":0.9359999895095825},{"id":"https://openalex.org/keywords/design-principles","display_name":"design principles","score":0.5720000267028809},{"id":"https://openalex.org/keywords/design-build-test-cycle","display_name":"design-build-test cycle","score":0.6539999842643738}],"concepts":[{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.8419362306594849},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.8020718097686768},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.6899600028991699},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.577365517616272},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.5618941187858582},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5376698970794678},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.5189759135246277},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.5082942843437195},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.47869327664375305},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.4707164168357849},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.40266162157058716},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.38472476601600647},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.36899417638778687},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2841230630874634}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013386","descriptor_name":"Succinates","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013386","descriptor_name":"Succinates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D055785","descriptor_name":"Gene Knockdown Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/sb4001432","doi":"https://doi.org/10.1021/sb4001432","is_oa":false,"landing_page_url":"https://doi.org/10.1021/sb4001432","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},{"id":"pmid:24328131","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24328131","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS synthetic biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332195","display_name":"Samsung","ror":"https://ror.org/04w3jy968"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W160114294","https://openalex.org/W1499220571","https://openalex.org/W1645305843","https://openalex.org/W1966543080","https://openalex.org/W1967210731","https://openalex.org/W1972549326","https://openalex.org/W1990606648","https://openalex.org/W2006745281","https://openalex.org/W2031708834","https://openalex.org/W2038492783","https://openalex.org/W2056375209","https://openalex.org/W2057594190","https://openalex.org/W2061315970","https://openalex.org/W2072369409","https://openalex.org/W2077489356","https://openalex.org/W2088631531","https://openalex.org/W2110071847","https://openalex.org/W2110647335","https://openalex.org/W2116424844","https://openalex.org/W2121987905","https://openalex.org/W2127412690","https://openalex.org/W2128795927","https://openalex.org/W2137003403","https://openalex.org/W2150972545","https://openalex.org/W2154797402","https://openalex.org/W2165612309","https://openalex.org/W2804007336","https://openalex.org/W7097514665"],"related_works":["https://openalex.org/W3039839287","https://openalex.org/W2931898804","https://openalex.org/W2801954748","https://openalex.org/W1185154679","https://openalex.org/W2576235672","https://openalex.org/W3015229134","https://openalex.org/W2006266631","https://openalex.org/W7073942989","https://openalex.org/W2012245873","https://openalex.org/W2911769745"],"abstract_inverted_index":{"Reduction":[0],"of":[1,9,71,105,111,128,142,152],"endogenous":[2],"gene":[3,16,36],"expression":[4,114],"is":[5,65],"a":[6,39,69,83,94,103],"fundamental":[7],"operation":[8],"metabolic":[10,45,95],"engineering,":[11],"yet":[12,90],"current":[13],"methods":[14],"for":[15,108,126],"knockdown":[17,37,50,143],"(i.e.,":[18],"genome":[19,61],"editing)":[20],"remain":[21],"laborious":[22],"and":[23,34,86,118,145,148],"slow,":[24],"especially":[25],"in":[26,52,68,79,116,132,156],"yeast.":[27,157],"In":[28,98],"contrast,":[29],"RNA":[30,113,120],"interference":[31,121],"allows":[32],"facile":[33],"tunable":[35],"via":[38],"simple":[40],"plasmid":[41],"transformation":[42],"step,":[43],"enabling":[44],"engineers":[46],"to":[47,59,122],"rapidly":[48],"prototype":[49],"strategies":[51,144],"multiple":[53],"strains":[54],"before":[55],"expending":[56],"significant":[57],"cost":[58,151],"undertake":[60],"editing.":[62],"Although":[63],"RNAi":[64],"naturally":[66],"present":[67],"myriad":[70],"eukaryotes,":[72],"it":[73],"has":[74,88],"only":[75],"been":[76,91],"recently":[77],"implemented":[78],"Saccharomyces":[80],"cerevisiae":[81],"as":[82,93],"heterologous":[84],"pathway":[85],"so":[87],"not":[89],"optimized":[92],"engineering":[96],"tool.":[97],"this":[99,133],"study,":[100],"we":[101],"elucidate":[102],"set":[104],"design":[106],"principles":[107],"the":[109,150,153],"construction":[110],"hairpin":[112],"cassettes":[115],"yeast":[117],"implement":[119],"quickly":[123],"identify":[124],"routes":[125],"improvement":[127],"itaconic":[129],"acid":[130],"production":[131],"organism.":[134],"The":[135],"approach":[136],"developed":[137],"here":[138],"enables":[139],"rapid":[140],"prototyping":[141],"thus":[146],"accelerates":[147],"reduces":[149],"design-build-test":[154],"cycle":[155]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":9},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":11},{"year":2014,"cited_by_count":7}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}