{"id":"https://openalex.org/W231599236","doi":null,"title":"Development of Secretion System for Heterologous Recombinant Protein Expression in E.coli Bacteria and Pichia pastoris Yeast","display_name":"Development of Secretion System for Heterologous Recombinant Protein Expression in E.coli Bacteria and Pichia pastoris Yeast","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W231599236","mag":"231599236"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt299047nt","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/299047nt","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100528501","display_name":"He Qu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Qu He","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5100528501"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.8970000147819519,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.8970000147819519,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.06040000170469284,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.008200000040233135,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sumoylation","display_name":"SUMOylation","score":0.7300000190734863},{"id":"https://openalex.org/keywords/pias1","display_name":"PIAS1","score":0.777999997138977},{"id":"https://openalex.org/keywords/e3-ubiquitin-ligase","display_name":"E3 ubiquitin ligase","score":0.8519999980926514},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.7300000190734863},{"id":"https://openalex.org/keywords/secretion-systems","display_name":"secretion systems","score":0.6620000004768372},{"id":"https://openalex.org/keywords/type-ii-secretion-system","display_name":"type II secretion system","score":0.9739999771118164},{"id":"https://openalex.org/keywords/sec-pathway","display_name":"Sec pathway","score":0.8460000157356262},{"id":"https://openalex.org/keywords/tat-pathway","display_name":"Tat pathway","score":0.9810000061988831},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.8999999761581421},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8690000176429749},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.7760000228881836}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.6230373978614807},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6054232120513916},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5388348698616028},{"id":"https://openalex.org/C171350616","wikidata":"https://www.wikidata.org/wiki/Q61506","display_name":"SUMO protein","level":4,"score":0.5306636095046997},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5294197201728821},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.5221067667007446},{"id":"https://openalex.org/C2780115692","wikidata":"https://www.wikidata.org/wiki/Q5747302","display_name":"Heterologous","level":3,"score":0.46336764097213745},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.29187217354774475},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.266650527715683},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08958446979522705}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt299047nt","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/299047nt","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:231599236","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt299047nt/qt299047nt.pdf?t=n4l006","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":94,"referenced_works":["https://openalex.org/W1520109974","https://openalex.org/W1602847006","https://openalex.org/W1885574814","https://openalex.org/W1888931068","https://openalex.org/W1966164969","https://openalex.org/W1968522752","https://openalex.org/W1970061483","https://openalex.org/W1970228759","https://openalex.org/W1970756887","https://openalex.org/W1973476447","https://openalex.org/W1976215395","https://openalex.org/W1978783141","https://openalex.org/W1980889490","https://openalex.org/W1981964231","https://openalex.org/W1983974405","https://openalex.org/W1985723042","https://openalex.org/W1991245779","https://openalex.org/W1991890196","https://openalex.org/W1992707998","https://openalex.org/W1993520606","https://openalex.org/W1994545731","https://openalex.org/W1995456120","https://openalex.org/W1995871176","https://openalex.org/W1997854324","https://openalex.org/W2000266693","https://openalex.org/W2001274114","https://openalex.org/W2004214053","https://openalex.org/W2004696942","https://openalex.org/W2004722555","https://openalex.org/W2007743853","https://openalex.org/W2013108773","https://openalex.org/W2013667867","https://openalex.org/W2016510254","https://openalex.org/W2023853843","https://openalex.org/W2024013245","https://openalex.org/W2024935012","https://openalex.org/W2028819914","https://openalex.org/W2031453541","https://openalex.org/W2038720022","https://openalex.org/W2040825545","https://openalex.org/W2041810719","https://openalex.org/W2051403769","https://openalex.org/W2052777309","https://openalex.org/W2056024151","https://openalex.org/W2061385538","https://openalex.org/W2061630866","https://openalex.org/W2062502129","https://openalex.org/W2063070929","https://openalex.org/W2063554961","https://openalex.org/W2066804008","https://openalex.org/W2067627598","https://openalex.org/W2071768512","https://openalex.org/W2076983776","https://openalex.org/W2079056345","https://openalex.org/W2082581989","https://openalex.org/W2083073535","https://openalex.org/W2083288707","https://openalex.org/W2083622405","https://openalex.org/W2085338944","https://openalex.org/W2087028483","https://openalex.org/W2089677512","https://openalex.org/W2089932939","https://openalex.org/W2090506411","https://openalex.org/W2093975193","https://openalex.org/W2094209629","https://openalex.org/W2094349654","https://openalex.org/W2096022379","https://openalex.org/W2096338485","https://openalex.org/W2102501687","https://openalex.org/W2108575112","https://openalex.org/W2108742330","https://openalex.org/W2109951903","https://openalex.org/W2112441454","https://openalex.org/W2114591766","https://openalex.org/W2120357935","https://openalex.org/W2121428233","https://openalex.org/W2122351989","https://openalex.org/W2128366869","https://openalex.org/W2129609211","https://openalex.org/W2131814796","https://openalex.org/W2132456915","https://openalex.org/W2132584326","https://openalex.org/W2137490166","https://openalex.org/W2143948627","https://openalex.org/W2151567065","https://openalex.org/W2151777569","https://openalex.org/W2160224841","https://openalex.org/W2166987747","https://openalex.org/W2167536088","https://openalex.org/W2297924974","https://openalex.org/W2332271182","https://openalex.org/W2791163698","https://openalex.org/W4231163667","https://openalex.org/W7005119639"],"related_works":["https://openalex.org/W2044109531","https://openalex.org/W1973961462","https://openalex.org/W7052261671","https://openalex.org/W2609633876","https://openalex.org/W2057771459","https://openalex.org/W2470053203","https://openalex.org/W57602035","https://openalex.org/W2145145345","https://openalex.org/W2597736866","https://openalex.org/W4200589919"],"abstract_inverted_index":{"small":[0],"ubiquitin-like":[1],"modifier":[2],"(SUMO),":[3],"plays":[4],"a":[5,62,66,104,187,191],"crucial":[6],"role":[7],"in":[8,30,38,46,169],"many":[9,47],"cellular":[10],"activities,":[11],"such":[12],"as":[13],"regulation":[14],"of":[15,41,72,92,107,113],"cell":[16,182],"cycle,":[17],"transcription,":[18],"apoptosis":[19],"and":[20,34,57,81,147,161],"DNA":[21],"damage":[22],"response.":[23],"SUMOylation":[24,42,59],"pathway":[25,43,60],"is":[26,44,61,100,103,186,190],"an":[27],"important":[28],"mechanism":[29],"modifying":[31],"protein":[32,36,105,167,198],"function":[33],"regulates":[35],"activities":[37],"eukaryotes.":[39],"Deregulation":[40],"found":[45],"diseases,":[48],"for":[49,194],"instance,":[50],"neurodegenerative":[51],"disease,":[52],"human":[53],"cancers,":[54],"heart":[55],"disease":[56],"diabetes.":[58],"three-step":[63],"process":[64],"involves":[65],"multi-enzyme":[67],"catalyzed":[68],"cascade":[69],"which":[70,157],"consists":[71],"the":[73,93,116,125,131],"following":[74],"enzymatic":[75],"steps:":[76],"activation":[77],"(E1),":[78],"conjugation":[79],"(E2)":[80],"ligation":[82,87],"(E3).":[83],"Only":[84],"some":[85],"E3":[86,94],"proteins":[88,142,174],"are":[89],"understood.":[90],"One":[91],"ligase":[95],"types":[96],"have":[97],"extensively":[98],"studied":[99],"PIAS.":[101],"It":[102],"inhibitor":[106],"activated":[108],"STAT":[109],"(PIAS1).":[110],"Intracellular":[111],"expression":[112],"PIAS1":[114,127],"with":[115],"bacterial":[117],"cells":[118],"does":[119],"not":[120],"work":[121],"well":[122],"due":[123],"to":[124,137,164],"toxicity":[126],"can":[128],"create":[129],"within":[130],"cells.We":[132],"established":[133],"two":[134],"secretion":[135,145,149,155],"systems":[136],"test":[138],"SUMO-":[139],"related":[140],"recombinant":[141,166],"expression:":[143],"Bacteria":[144],"system":[146,156],"yeast":[148,181,185],"system.":[150],"We":[151],"used":[152],"type":[153],"II":[154],"includes":[158],"Sec,":[159],"Tat":[160],"SRP-":[162],"pathways":[163],"secreted":[165],"production":[168],"E.coli":[170],"K-12":[171],"strain.":[172],"Recombinant":[173],"also":[175],"been":[176],"tested":[177],"on":[178],"Pichia":[179],"pastoris":[180],"line.":[183],"Since":[184],"eukaryote,":[188],"it":[189],"suitable":[192],"host":[193],"produce":[195],"eukaryotic":[196],"heterologous":[197],"like":[199],"SUMO-related":[200],"proteins.":[201]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
