{"id":"https://openalex.org/W2300540835","doi":null,"title":"Caract\u00e9risation de l\u2019interaction entre la prot\u00e9ine virale VPg et la prot\u00e9ine de l\u2019h\u00f4te eIF4E conduisant \u00e0 l\u2019infection du virus de la mosa\u00efque du navet.","display_name":"Caract\u00e9risation de l\u2019interaction entre la prot\u00e9ine virale VPg et la prot\u00e9ine de l\u2019h\u00f4te eIF4E conduisant \u00e0 l\u2019infection du virus de la mosa\u00efque du navet.","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W2300540835","mag":"2300540835"},"language":"fr","primary_location":{"id":"pmh:oai:espace.inrs.ca:2164","doi":null,"is_oa":false,"landing_page_url":"https://espace.inrs.ca/id/eprint/2164/1/L%C3%A9onard%2C_Simon--combin%C3%A9.pdf","pdf_url":"https://espace.inrs.ca/id/eprint/2164/1/L%C3%A9onard%2C_Simon--combin%C3%A9.pdf","source":{"id":"https://openalex.org/S4306400579","display_name":"EspaceINRS (National Institute for Scientific Research (Canada))","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I39481719","host_organization_name":"Institut National de la Recherche Scientifique","host_organization_lineage":["https://openalex.org/I39481719"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Th\u00e8se"},"volume":null,"type":"dissertation","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Simon L\u00e9onard","orcid":"https://orcid.org/0000-0001-6429-4755","observed_orcids":["https://orcid.org/0000-0001-6429-4755"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Simon L\u00e9onard","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-6429-4755","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12856","display_name":"Transgenic Plants and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tomato-mosaic-virus","display_name":"tomato mosaic virus","score":0.7639999985694885},{"id":"https://openalex.org/keywords/vpg","display_name":"VPg","score":0.7509999871253967},{"id":"https://openalex.org/keywords/eif4e","display_name":"eIF4E","score":0.996999979019165},{"id":"https://openalex.org/keywords/virus-host-interactions","display_name":"host-virus interaction","score":0.7649999856948853},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.7200000286102295},{"id":"https://openalex.org/keywords/interaction-domains","display_name":"interaction domains","score":0.5370000004768372},{"id":"https://openalex.org/keywords/aspartate-residues","display_name":"aspartic acid residues","score":0.6710000038146973},{"id":"https://openalex.org/keywords/plant-virus-infection","display_name":"plant virus infection","score":0.38600000739097595},{"id":"https://openalex.org/keywords/potyvirus","display_name":"potyvirus","score":0.75},{"id":"https://openalex.org/keywords/viral-rna-translation","display_name":"viral RNA translation","score":0.5220000147819519},{"id":"https://openalex.org/keywords/viral-replication","display_name":"viral replication","score":0.4000000059604645},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8799999952316284},{"id":"https://openalex.org/keywords/tobacco-mosaic-virus","display_name":"tobacco mosaic virus","score":0.3310000002384186}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38384315371513367},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36837488412857056},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.32273608446121216},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2799665331840515}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:espace.inrs.ca:2164","doi":null,"is_oa":false,"landing_page_url":"https://espace.inrs.ca/id/eprint/2164/1/L%C3%A9onard%2C_Simon--combin%C3%A9.pdf","pdf_url":"https://espace.inrs.ca/id/eprint/2164/1/L%C3%A9onard%2C_Simon--combin%C3%A9.pdf","source":{"id":"https://openalex.org/S4306400579","display_name":"EspaceINRS (National Institute for Scientific Research (Canada))","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I39481719","host_organization_name":"Institut National de la Recherche Scientifique","host_organization_lineage":["https://openalex.org/I39481719"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Th\u00e8se"},{"id":"mag:2300540835","doi":null,"is_oa":false,"landing_page_url":"http://espace.inrs.ca/2164/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2345511685","https://openalex.org/W2342592157","https://openalex.org/W2395694231","https://openalex.org/W2563415388","https://openalex.org/W2146915367","https://openalex.org/W2064854185","https://openalex.org/W2976838371","https://openalex.org/W2058023101","https://openalex.org/W7161966262","https://openalex.org/W7017831960"],"abstract_inverted_index":{"La":[0,25,433],"transcription":[1],"des":[2,5,200,302,441,492,498,504,515,716,756],"symboles":[3],"et":[4,121,273,298,361,391,405,474,510,528,547,596,644,677,711],"caract\u00e8res":[6],"sp\u00e9ciaux":[7],"utilis\u00e9s":[8],"dans":[9,173,309,373],"la":[10,38,102,109,138,153,164,185,198,218,232,243,266,274,300,318,337,362,377,380,389,406,414,425,449,476,508,511,522,529,545,548,563,573,577,588,594,597,606,610,614,618,621,624,673,692,719,730,747,754,759],"version":[11,26],"originale":[12],"de":[13,22,28,37,52,79,123,127,132,137,144,150,163,170,184,208,224,245,256,261,265,296,299,317,331,376,413,435,455,478,483,535,570,572,587,603,608,633,642,699,741],"ce":[14,29,74,174,310,346,489,652,743],"r\u00e9sum\u00e9":[15,30],"n\u2019a":[16],"pas":[17,229,591,650],"\u00e9t\u00e9":[18,87,704,713,727],"possible":[19],"en":[20,34,90,146,181,314,502,664],"raison":[21],"limitations":[23],"techniques.":[24],"correcte":[27],"peut":[31,751],"\u00eatre":[32],"lue":[33],"PDF.Le":[35],"virus":[36,53,85,303,461,648,682,780],"mosa\u00efque":[39],"du":[40,84,100,288,339,364,370,382,397,416,579,635,675,679,684,694,721,749],"navet":[41],"(TuMV)":[42],"appartient":[43],"au":[44,334],"potyvirus":[45,371,643],"qui":[46,490,744],"est":[47,491,558,668,737,770],"le":[48,77,94,106,134,206,374,555,670,776],"plus":[49],"important":[50],"groupe":[51,653],"infectant":[54],"les":[55,58,71,114,119,239,446,518,645,779],"plantes.":[56,115],"Cependant,":[57,599],"m\u00e9canismes":[59],"mol\u00e9culaires":[60],"conduisant":[61,81],"\u00e0":[62,82,215,221,235,252,304,329,438,521,600,623,651,729,739,774],"une":[63,155,356,465,541],"infection":[64,353],"virale":[65,354,575],"sont":[66,393,408,485,654],"tr\u00e8s":[67,655],"peu":[68,482],"connus":[69],"chez":[70,113,152],"potyvirus.":[72,493],"Dans":[73],"pr\u00e9sent":[75],"ouvrage,":[76],"processus":[78,207],"r\u00e9plication/traduction":[80,634],"l'infection":[83,330],"a":[86,726],"particuli\u00e8rement":[88],"approfondi":[89],"utilisant":[91,503],"comme":[92,187,507,525],"mod\u00e8le":[93,632],"TuMV.":[95],"\\nNous":[96],"savons":[97],"qu'une":[98],"prot\u00e9ine":[99],"TuMV,":[101,289],"VPg,":[103,219],"interagit":[104,444],"avec":[105,166,192,231,323,445,517,562,617,697,706,718,753,761],"facteur":[107],"initiant":[108,448],"traduction":[110,209,426,450,523],"cellulaire":[111,427,443,451,524],"4E":[112],"Nous":[116,211,247,306,325,385,494,565],"ignorons":[117],"cependant":[118],"cons\u00e9quences":[120],"l'implication":[122],"cette":[124,735],"interaction.":[125],"Un":[126],"nos":[128],"objectifs":[129],"fut":[130],"donc":[131,496],"caract\u00e9riser":[133],"domaine":[135,175,556,724],"d'interaction":[136,725],"VPg":[139,154,186,319],"pour":[140,190,203,242,293,321,470,488,560,620,758,778],"l'eiF4E.":[141,167,324,762],"Des":[142,168],"travaux":[143,688],"d\u00e9l\u00e9tions":[145],"double-hybride":[147],"ont":[148,176,552,689,703,712],"permis":[149],"cibler":[151],"r\u00e9gion":[156],"centrale":[157],"d'une":[158,352],"trentaine":[159],"d'acides":[160],"amin\u00e9s":[161],"responsables":[162],"liaison":[165,244,622,760],"\u00e9tudes":[169,422],"mutag\u00e9n\u00e8ses":[171],"dirig\u00e9es":[172],"\u00e9galement":[177,567,766],"identifi\u00e9":[178],"l'acide":[179,312],"aspartique":[180,313],"position":[182,315],"77":[183,316],"\u00e9tant":[188],"n\u00e9cessaire":[189,320],"l'interaction":[191,270,322,359,561,592,768],"l'e1F4E.":[193],"\\n":[194,195,276,277,278,366,367,418,419,638,639],"\\nL'eiF4E":[196],"reconna\u00eet":[197],"coiffe":[199,233,755],"ARNm":[201,240,442,757],"cellulaires":[202,241],"ensuite":[204,328],"enclencher":[205],"cellulaire.":[210],"nous":[212,281,538,628],"sommes":[213],"interrog\u00e9s":[214],"savoir":[216],"si":[217],"situ\u00e9e":[220,234],"l'extr\u00e9mit\u00e9":[222,236,439],"5'":[223],"l'ARN":[225],"viral,":[226],"ne":[227,348],"pourrait":[228],"comp\u00e9titionner":[230],"5'de":[237],"tous":[238],"l'eIF4E.":[246],"avons":[248,282,307,326,386,495,539,566],"montr\u00e9":[249,387,540],"in":[250,500],"vitro":[251,501],"l'aide":[253,532,707],"d'un":[254,285,533,708],"test":[255,534,709],"type":[257,536],"\\nELISA":[258],"que":[259,388,403,424,554,585,613,691,746,767],"l'ajout":[260,571,740],"m7GTP":[262],"(un":[263],"analogue":[264],"coiffe)":[267],"diminue":[268],"grandement":[269],"entre":[271,358,544,593,672],"l'eiF4E":[272,392],"VPgPro.":[275,598,625],"\\nDe":[279,420],"plus,":[280],"fait":[283],"acquisition":[284],"plasmide":[286],"infectieux":[287],"un":[290,430,631,771],"outil":[291],"indispensable":[292],"\u00e9tudier":[294],"l'\u00e9tude":[295],"l'expression":[297],"r\u00e9plication":[301,369,415],"ARN(+).":[305],"mut\u00e9,":[308],"plasmide,":[311],"proc\u00e9d\u00e9":[327],"plantes":[332,343],"sensibles":[333],"TuMV":[335,636,676],"par":[336,345,453,715],"technique":[338,720],"bombardement":[340],"particulaire.":[341],"Les":[342],"infect\u00e9es":[344],"clone":[347],"pr\u00e9sentaient":[349],"aucun":[350],"sympt\u00f4me":[351],"sugg\u00e9rant":[355,612],"corr\u00e9lation":[357],"VPgMeIF4E":[360],"virulence":[363],"virus.":[365,417],"\\nLa":[368],"s'assemble":[372],"cytosol":[375],"cellule":[378],"sur":[379,576],"membrane":[381],"r\u00e9ticulum":[383,398],"endoplasmique.":[384,399],"6KVPgPro":[390],"coMlocalis\u00e9es":[394],"aux":[395],"membranes":[396],"Ce":[400],"r\u00e9sultat":[401],"sugg\u00e8re":[402,745],"l'eIF4E":[404],"VPgPro":[407,509,546,641,646,674,693,748],"toutes":[409],"deux":[410],"impliqu\u00e9s":[411],"lors":[412],"nombreuses":[421],"d\u00e9montrent":[423,584],"s'effectue":[428],"selon":[429],"m\u00e9canisme":[431,772],"circulaire.":[432],"queue":[434],"poly(A)":[436],"pr\u00e9sente":[437],"3'":[440],"prot\u00e9ines":[447,479,505,519],"(eiF)":[452],"l'interm\u00e9diaire":[454],"lapoly(A)":[456],"binding":[457],"protein":[458],"(PABP).":[459],"Plusieurs":[460],"d'animaux":[462],"utilisent":[463],"aussi":[464],"strat\u00e9gie":[466],"similaire":[467],"(la":[468],"circularisation)":[469],"traduire":[471],"leur":[472,659,662],"ARN":[473],"maximiser":[475],"production":[477],"virales.":[480],"Bien":[481],"donn\u00e9es":[484],"toutefois":[486],"connues":[487],"effectu\u00e9":[497],"exp\u00e9riences":[499],"virales,":[506],"Polym\u00e9rase,":[512],"afin":[513],"d'\u00e9tablir":[514,630],"liens":[516],"n\u00e9cessaires":[520],"l'eiF4E,":[526],"eiF4G":[527],"PABP.":[530,549,564],"Avec":[531],"ELISA,":[537,734],"nouvelle":[542],"interaction":[543,736],"Nos":[550,582,687],"r\u00e9sultats":[551,583,627,702,764],"indiqu\u00e9":[553,690],"Pro":[557],"requis":[559],"v\u00e9rifi\u00e9":[568],"l'effet":[569],"polym\u00e9rase":[574,589,607,619],"formation":[578],"complexe":[580],"VPgPro-PABP.":[581],"l'addition":[586,602],"n'affecte":[590],"PABP":[595,604,615],"l'inverse,":[601],"emp\u00eache":[605],"lier":[609],"VPgPro,":[611],"comp\u00e9titionne":[616],"Ces":[626,701,763],"permettent":[629],".":[637],"\\nLes":[640],"d'autres":[647],"n'appartenant":[649],"variables":[656],"lorsqu'on":[657],"compare":[658],"taille":[660],"ou":[661],"s\u00e9quence":[663],"acides":[665],"amin\u00e9s.":[666],"Ceci":[667],"notamment":[669],"cas":[671],"celle":[678],"tomato":[680],"ringspot":[681],"(ToRSV)":[683],"genre":[685],"n\u00e9povirus.":[686],"n\u00e9povirus":[695,750],"interagissait":[696],"l'eiF(iso)4E":[698],"bl\u00e9.":[700],"montr\u00e9s":[705],"ELISA":[710],"confirm\u00e9s":[714],"collaborateurs":[717],"Far-Western.":[722],"Le":[723],"cibl\u00e9":[728],"partie":[731],"Pro.":[732],"En":[733],"sensible":[738],"\\nM7GTP,":[742],"comp\u00e9tionner":[752],"sugg\u00e8rent":[765],"VPgPro-eiF4E":[769],"universel,":[773],"tout":[775],"moins":[777],"phytopathog\u00e8nes.":[781]},"counts_by_year":[],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2016-06-24T00:00:00"}